3D (three-dimensional) culture device based on umbilical cord stem cell culture

Abstract

The utility model relates to the technical field of umbilical cord stem cell culture, and discloses an umbilical cord stem cell culture-based 3D (three-dimensional) culture device which comprises a shell, universal wheels arranged at the bottom of the shell, a protective door arranged on the front side of the shell, a mounting column rotatably mounted in the shell, and a mounting disc fixedly mounted outside the mounting column, an auxiliary culture mechanism is arranged at the top of the mounting disc, a culture dish is arranged at the top of the auxiliary culture mechanism, and a sterilization mechanism is arranged in the shell. By arranging the auxiliary culture mechanism, in the using process, a placed culture dish can be stably clamped, and when the culture condition of umbilical cord stem cells in the culture dish needs to be checked for observation, the culture dish can be adjusted and displayed at a proper angle under the condition that the culture dish is not taken out; before the culture dish is placed, the interior of the device can be disinfected and sterilized, and the problem that infectious microbes interfere culture of the culture dish is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of umbilical cord stem cell culture, in particular to a 3D stereoscopic culture device for umbilical cord stem cell culture. Background Art

[0002] Umbilical cord stem cells are often used to treat blood system diseases, innate immune diseases, tumors, etc. They can resist cancer cells and play a role in tissue cell repair. Special culture devices are often used in the medical culture process.

[0003] According to the description of a stem cell culture device disclosed on the patent website (authorization announcement number: CN209443016U), "The utility model discloses a stem cell culture device, which relates to the field of cell biology. The stem cell culture device includes a box body and a plurality of culture bottles stacked on each other inside the box body. The box body is provided with a plurality of side surfaces and a primary and secondary observation windows. The culture bottles can be conveniently taken out through the side surfaces, and the internal environment and cell growth conditions can be conveniently observed through the primary and secondary observation windows. The utility model adopts a new type of horizontal culture bottle. The sealed bottle cap of the culture bottle can isolate external bacteria, so that the injection process of stem cells is in a sterile environment. At the same time, the breathable bottle cap of the culture bottle can effectively balance the air pressure inside the culture bottle and exchange the oxygen inside and outside the bottle. The stem cell culture device includes a plurality of culture bottles stacked on each other. This stacking arrangement can improve space utilization and facilitate observation."

[0004] In view of the above description, the applicant believes that there are the following problems:

[0005] During use, the utility model can conveniently take out the culture bottles through the sides through the multiple side surfaces and the main and auxiliary observation windows, and conveniently observe the internal environment and cell growth conditions through the main and auxiliary observation windows. However, in actual applications, when the device contains too many culture bottles, it is difficult to observe the culture conditions inside a single culture bottle through the main and auxiliary observation windows, and the device does not disinfect and sterilize the inside of the device before placing the culture bottles. After the culture bottles are placed, miscellaneous bacteria can easily contaminate the culture bottles, resulting in unsatisfactory culture effects. Therefore, we urgently need a 3D stereoscopic culture device for umbilical cord stem cell culture to solve the above problems. Utility Model Content

[0006] The purpose of the utility model is to provide a 3D culture device for culturing umbilical cord stem cells to solve the problems raised in the above-mentioned background technology.

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a 3D stereoscopic culture device for umbilical cord stem cell culture, comprising a casing, a universal wheel is arranged at the bottom of the casing, a protective door is arranged at the front of the casing, a mounting column is rotatably installed inside the casing, a mounting plate is fixedly installed outside the mounting column, an auxiliary culture mechanism is arranged on the top of the mounting plate, a culture dish is arranged on the top of the auxiliary culture mechanism, and a sterilization mechanism is arranged inside the casing.

[0008] The auxiliary culture mechanism comprises an auxiliary component and a placing component, and the auxiliary component is arranged at the bottom of the placing component.

[0009] Preferably, the auxiliary component includes a connecting column, which is fixedly installed inside the mounting plate, a first mounting block is rotatably installed outside the connecting column, a pressing plate is fixedly installed on the top of the first mounting block, a fixing plate is fixedly installed on the bottom of the mounting plate, a supporting column is fixedly installed on the bottom of the first mounting block, and a first return spring is fixedly installed on the bottom of the first mounting block, so that when observing the culture condition of umbilical cord stem cells inside the culture dish, the culture dish can be adjusted to an appropriate angle for display without taking out the culture dish.

[0010] Preferably, mounting grooves are provided at corresponding positions of the mounting plate and the auxiliary component, the support column and the top of the first return spring are fixedly mounted to the first mounting block, and the support column and the bottom of the first return spring are fixedly mounted to the top of the fixed plate, so that the culture dish can be rotated at an appropriate angle when it is necessary to check the internal situation of the culture dish.

[0011] Preferably, the placement assembly includes a mounting plate, which is fixedly mounted on the top of the first mounting block, a second mounting block is fixedly mounted on the top of the mounting plate, a sliding rod is slidably mounted inside the second mounting block, a limiting baffle is fixedly mounted on the side of the sliding rod away from the second mounting block, a second return spring is sleeved on the outside of the sliding rod, a positioning plate is fixedly mounted on the outside of the sliding rod, and a soft pad is fixedly mounted on the side of the positioning plate away from the sliding rod, so as to stably clamp the placed culture dish and prevent the culture dish from falling off the device and being damaged during movement.

[0012] Preferably, two groups of the second mounting blocks are provided, and the two groups of the second mounting blocks are symmetrically arranged on the top of the mounting plate. The second return spring is fixedly installed with the limit baffle near one side of the limit baffle, and the second return spring is fixedly installed with the second mounting block near one side of the second mounting block, so as to form a stable clamping for culture dishes of different sizes.

[0013] Preferably, the sterilization mechanism includes a motor seat, which is fixedly mounted on the top of the casing, a first motor is fixedly mounted inside the motor seat, a screw is fixedly mounted on the output shaft of the first motor, a guide column is fixedly mounted inside the casing, a movable frame is threadedly mounted on the outer side of the screw, and a sterilization lamp is fixedly mounted inside the movable frame, so as to facilitate disinfection and sterilization of the inside of the device before placing the culture dish, thereby preventing the occurrence of miscellaneous bacteria from interfering with the culture of the culture dish.

[0014] Preferably, a slide groove is opened at the corresponding position of the movable frame and the guide column passes through the movable frame, a through hole is opened at the corresponding position of the top of the casing and the first motor output shaft, and the first motor output shaft passes through the through hole, so as to facilitate the up and down reciprocating disinfection and sterilization of the inside of the casing.

[0015] Compared with the prior art, the utility model provides a 3D culture device for umbilical cord stem cell culture, which has the following beneficial effects:

[0016] 1. The 3D culture device for umbilical cord stem cell culture can stably clamp the placed culture dish during use by setting an auxiliary culture mechanism to prevent the culture dish from falling from the device and being damaged during movement. When it is necessary to observe the umbilical cord stem cell culture conditions inside the culture dish, the culture dish can be adjusted to an appropriate angle for display without taking out the culture dish.

[0017] 2. The 3D culture device for umbilical cord stem cell culture is provided with a sterilization mechanism. During use, before placing the culture dish, the sterilization lamp can be reciprocated up and down inside the casing to disinfect and sterilize the inside of the device, thereby preventing miscellaneous bacteria from interfering with the culture of the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative labor:

[0019] Figure 1 This is a schematic diagram of the overall appearance structure of the utility model;

[0020] Figure 2 This is a schematic diagram of the front cross-sectional structure of the utility model;

[0021] Figure 3 This is a schematic diagram of the overall structure of the auxiliary training mechanism of the utility model;

[0022] Figure 4 This is a schematic diagram of the disassembled structure of the auxiliary components of the utility model;

[0023] Figure 5 This is a schematic diagram of the disassembled structure of the placement components of the utility model;

[0024] Figure 6 This is a schematic diagram of the disassembled structure of the sterilization mechanism of the utility model.

[0025] In the figure: 1. casing; 2. universal wheel; 3. protective door; 4. mounting column; 5. mounting plate; 6. auxiliary culture mechanism; 61. auxiliary component; 611. connecting column; 612. first mounting block; 613. pressing plate; 614. fixing plate; 615. supporting column; 616. first return spring; 62. placing component; 621. mounting plate; 622. second mounting block; 623. sliding rod; 624. limiting baffle; 625. second return spring; 626. positioning plate; 627. cushion; 7. culture dish; 8. sterilization mechanism; 81. motor seat; 82. first motor; 83. screw; 84. guide column; 85. movable frame; 86. sterilization lamp. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] In the present invention, unless otherwise clearly specified and limited, the terms "install", "connect", "connect", "fix" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0028] Embodiment 1:

[0029] See also Figure 1-5The utility model provides a technical solution: a 3D stereoscopic culture device for umbilical cord stem cell culture, comprising a casing 1, a universal wheel 2 is arranged at the bottom of the casing 1, a protective door 3 is arranged at the front of the casing 1, a mounting column 4 is rotatably installed inside the casing 1, a mounting plate 5 is fixedly installed outside the mounting column 4, an auxiliary culture mechanism 6 is arranged on the top of the mounting plate 5, a culture dish 7 is arranged on the top of the auxiliary culture mechanism 6, and a sterilization mechanism 8 is arranged inside the casing 1.

[0030] The auxiliary culture mechanism 6 includes an auxiliary component 61 and a placement component 62 , and the auxiliary component 61 is arranged at the bottom of the placement component 62 .

[0031] Furthermore, the auxiliary component 61 includes a connecting column 611, which is fixedly installed inside the mounting plate 5, and a first mounting block 612 is rotatably installed outside the connecting column 611, a pressing plate 613 is fixedly installed on the top of the first mounting block 612, a fixing plate 614 is fixedly installed on the bottom of the mounting plate 5, a supporting column 615 is fixedly installed on the bottom of the first mounting block 612, and a first return spring 616 is fixedly installed on the bottom of the first mounting block 612, so that when observing the culture condition of umbilical cord stem cells inside the culture dish 7, the culture dish 7 can be adjusted to an appropriate angle for display without taking out the culture dish 7.

[0032] Furthermore, mounting grooves are provided at corresponding positions of the mounting plate 5 and the auxiliary component 61, the support column 615 and the top of the first return spring 616 are fixedly mounted to the first mounting block 612, and the support column 615 and the bottom of the first return spring 616 are fixedly mounted to the top of the fixed plate 614, so that the culture dish 7 can be rotated at an appropriate angle when it is necessary to check the internal condition of the culture dish 7.

[0033] Furthermore, the placement component 62 includes a mounting plate 621, which is fixedly mounted on the top of the first mounting block 612, a second mounting block 622 is fixedly mounted on the top of the mounting plate 621, a slide rod 623 is slidably mounted inside the second mounting block 622, a limit baffle 624 is fixedly mounted on the side of the slide rod 623 away from the second mounting block 622, a second return spring 625 is sleeved on the outside of the slide rod 623, a positioning plate 626 is fixedly mounted on the outside of the slide rod 623, and a soft pad 627 is fixedly mounted on the side of the positioning plate 626 away from the slide rod 623, so as to stably clamp the placed culture dish 7 and prevent the culture dish 7 from falling off the device and being damaged during movement.

[0034] Furthermore, two groups of second mounting blocks 622 are provided, and the two groups of second mounting blocks 622 are symmetrically arranged on the top of the mounting plate 621. The second return spring 625 is fixedly installed with the limit baffle 624 near one side of the limit baffle 624, and the second return spring 625 is fixedly installed with the second mounting block 622 near one side of the second mounting block 622, so as to form a stable clamping for culture dishes 7 of different sizes.

[0035] Embodiment 2:

[0036] See also Figure 6 , and combined with Example 1, it is further obtained that the sterilization mechanism 8 includes a motor base 81, the motor base 81 is fixedly installed on the top of the casing 1, a first motor 82 is fixedly installed inside the motor base 81, a screw 83 is fixedly installed on the output shaft of the first motor 82, a guide column 84 is fixedly installed inside the casing 1, a movable frame 85 is threadedly installed on the outer surface of the screw 83, and a sterilization lamp 86 is fixedly installed inside the movable frame 85, which is convenient for disinfecting and sterilizing the inside of the device before placing the culture dish 7 to prevent the interference of miscellaneous bacteria on the culture of the culture dish 7.

[0037] Furthermore, a slide groove is opened at the corresponding position of the movable frame 85 and the guide column 84, and the guide column 84 passes through the movable frame 85. A through hole is opened at the top of the casing 1 and at the corresponding position of the output shaft of the first motor 82, and the output shaft of the first motor 82 passes through the through hole, so as to facilitate the up and down reciprocating disinfection and sterilization of the inside of the casing 1.

[0038] In actual operation, when the device is used, the first motor 82 is turned on to drive the screw 83 to rotate, and then the movable frame 85 is driven to move outside the screw 83 under the action of the guide column 84, thereby driving the sterilization lamp 86 inside the movable frame 85 to move up and down, so as to evenly disinfect and sterilize the inside of the casing 1 to prevent the influence on the umbilical cord stem cell culture in the later stage. After the disinfection is completed, the culture dish 7 containing the umbilical cord stem cells is placed on the top of the mounting plate 621, and the culture dish 7 will touch the soft pads 627 on both sides, which will push the slide bar 623 to move outward on both sides. When the placement is completed, under the action of the second return spring 625, the positioning plates 626 and the soft pads 627 on both sides will be moved outward. The pad 627 forms a stable clamping position for the culture dish 7, and then the mounting plate 5 is rotated to place multiple groups of culture dishes 7, thereby expanding the utilization of the internal space of the casing 1. When it is necessary to check the internal situation of the culture dish 7 during the culture process, the pressing plate 613 can be pressed down, and the other side can be driven to rotate under the action of the connecting column 611 and the first reset spring 616, so that the culture dish 7 is lifted up, which is convenient for observing the internal situation of the culture dish 7. After the inspection is completed, under the action of the first reset spring 616, the culture dish 7 will slowly return to the initial position, and under the action of the support column 615, the culture dish 7 is kept stable, and the entire device can be moved by the universal wheel 2 set at the bottom of the device.

[0039] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the statement "comprise a ..." do not exclude the presence of other identical elements in the process, method, article or device including the elements.

Claims

1. A 3D culture device for culturing umbilical cord stem cells, comprising a housing (1), characterized in that: The bottom of the housing (1) is provided with a universal wheel (2), the front of the housing (1) is provided with a protective door (3), a mounting column (4) is rotatably mounted inside the housing (1), a mounting plate (5) is fixedly mounted outside the mounting column (4), an auxiliary culture mechanism (6) is arranged on the top of the mounting plate (5), a culture dish (7) is arranged on the top of the auxiliary culture mechanism (6), and a sterilization mechanism (8) is arranged inside the housing (1); The auxiliary culture mechanism (6) comprises an auxiliary component (61) and a placement component (62), wherein the auxiliary component (61) is arranged at the bottom of the placement component (62).

2. A 3D culture device for umbilical cord stem cell culture according to claim 1, characterized in that: The auxiliary component (61) comprises a connecting column (611), wherein the connecting column (611) is fixedly mounted inside the mounting plate (5), a first mounting block (612) is rotatably mounted outside the connecting column (611), a pressing plate (613) is fixedly mounted on the top of the first mounting block (612), a fixing plate (614) is fixedly mounted on the bottom of the mounting plate (5), a supporting column (615) is fixedly mounted on the bottom of the first mounting block (612), and a first return spring (616) is fixedly mounted on the bottom of the first mounting block (612).

3. A 3D culture device for umbilical cord stem cell culture according to claim 2, characterized in that: The mounting plate (5) and the auxiliary component (61) are provided with mounting grooves at positions corresponding to each other; the top of the support column (615) and the first return spring (616) are fixedly mounted to the first mounting block (612); and the bottom of the support column (615) and the first return spring (616) are fixedly mounted to the top of the fixing plate (614).

4. A 3D culture device for umbilical cord stem cell culture according to claim 1, characterized in that: The placement component (62) includes a mounting plate (621), the mounting plate (621) is fixedly mounted on the top of the first mounting block (612), a second mounting block (622) is fixedly mounted on the top of the mounting plate (621), a sliding rod (623) is slidably mounted inside the second mounting block (622), a limit stop plate (624) is fixedly mounted on the side of the sliding rod (623) away from the second mounting block (622), a second return spring (625) is sleeved and mounted on the outside of the sliding rod (623), a positioning plate (626) is fixedly mounted on the outside of the sliding rod (623), and a soft pad (627) is fixedly mounted on the side of the positioning plate (626) away from the sliding rod (623).

5. A 3D culture device for umbilical cord stem cell culture according to claim 4, characterized in that: The second mounting blocks (622) are provided in two groups, and the two groups of the second mounting blocks (622) are symmetrically arranged on the top of the mounting plate (621); the second return spring (625) is fixedly installed with the limit baffle (624) on one side close to the limit baffle (624); and the second return spring (625) is fixedly installed with the second mounting block (622) on one side close to the second mounting block (622).

6. A 3D culture device for umbilical cord stem cell culture according to claim 1, characterized in that: The sterilization mechanism (8) comprises a motor seat (81), the motor seat (81) is fixedly mounted on the top of the casing (1), a first motor (82) is fixedly mounted inside the motor seat (81), a screw (83) is fixedly mounted on the output shaft of the first motor (82), a guide column (84) is fixedly mounted inside the casing (1), a movable frame (85) is threadedly mounted on the outside of the screw (83), and a sterilization lamp (86) is fixedly mounted inside the movable frame (85).

7. A 3D culture device for umbilical cord stem cell culture according to claim 6, characterized in that: A sliding groove is provided at a position corresponding to the movable frame (85) and the guide column (84); the guide column (84) passes through the movable frame (85); a through hole is provided at a position corresponding to the output shaft of the first motor (82) at the top of the housing (1); and the output shaft of the first motor (82) passes through the through hole.

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