Solid adding device for protein purification salting-out

Abstract

The utility model discloses a solid adding device for protein purification salting-out, which relates to the technical field of protein purification and comprises a support frame, a feeding tank is arranged at the top of the support frame, a top cover is arranged at the top of the feeding tank, a crushing component is arranged at the bottom of the top cover, and a connecting component is arranged on one side of the top of the top cover. A feeding assembly is arranged in the feeding tank, the connecting assembly comprises a base, a rotating seat is arranged at the top of the base, a rotating rod is arranged at the top of the rotating seat, a connecting plate is arranged at the top of the rotating rod, and a positioning screw rod is arranged at one end of the connecting plate. Solid salt is crushed through the arranged crushing assembly, and the solid salt is crushed and ground through the arranged crushing cutter set and the arc-shaped grinding plate, so that subsequent weight detection is facilitated, feeding is facilitated, chemical agent splashing caused by the weight of blocky solid salt in the feeding process is prevented, and the feeding quality of the blocky solid salt is improved. And dangerous factors are reduced.

Description

Technical Field

[0001] The utility model relates to the field of protein purification technology, in particular to a solid adding device for protein purification and salting out. Background Art

[0002] Protein separation and purification is widely used in biochemical research applications and is an important operating technology. There are many methods for protein purification, among which salting-out protein purification is the most widely used. The principle of protein separation by salting-out is actually to reduce the solubility of protein, so that the protein coagulates together and slowly precipitates out of the solution. Protein precipitation is actually a phenomenon of separation, that is, aggregating protein molecules and causing them to precipitate out of the solution.

[0003] In the existing salting-out devices for protein purification, when adding salt for salting-out, most of them are done by manual crushing of solid salt, and most of them are added based on feeling or after weighing successively. The manual adding method based on feeling is prone to large errors, resulting in poor salting-out effect. The operation of adding by weighing successively is time-consuming, labor-intensive and inefficient. Therefore, it is necessary to provide a solid adding device for protein purification and salting-out to solve the problem. Utility Model Content

[0004] The main purpose of the utility model is to provide a solid adding device for protein purification and salting out, which can effectively solve the problems in the background technology.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] A solid addition device for protein purification and salting out comprises a support frame, a feeding tank is arranged on the top of the support frame, a top cover is arranged on the top of the feeding tank, a crushing component is arranged at the bottom of the top cover, a connecting component is arranged on one side of the top of the top cover, and a feeding component is arranged inside the feeding tank;

[0007] The connecting assembly comprises a base, a rotating seat is arranged on the top of the base, a rotating rod is arranged on the top of the rotating seat, a connecting plate is arranged on the top of the rotating rod, and a positioning screw is arranged at one end of the connecting plate;

[0008] The crushing assembly comprises a crushing tank, a rotating rod is arranged in the middle of the crushing tank, an arc-shaped grinding plate is arranged at the bottom of the rotating rod, a crushing knife group is arranged above the arc-shaped grinding plate, and a discharge pipe is arranged at the bottom of the crushing tank;

[0009] The feeding assembly includes a support seat, a feeding pipe is arranged above the support seat, a rotating shaft is arranged in the middle of the feeding pipe, a transmission propeller is arranged on the outer wall of the rotating shaft, a discharge pipe is arranged at the bottom of one end of the feeding pipe, a control valve is arranged inside one end of the discharge pipe, and a receiving plate is arranged inside the other end of the discharge pipe.

[0010] Preferably, the side of the base is fixedly connected to the outer wall of the feeding tank, and there are three bases, which are evenly distributed on the outer wall of the base. The top of the base is connected to the rotating seat by bolts, and the rotating seat is rotatably connected to the rotating rod.

[0011] Preferably, one end of the rotating rod away from the rotating seat is connected to the connecting plate by a bolt, and the end of the connecting plate away from the rotating rod is located above the top cover. The connecting plate is connected to the positioning screw by a thread, and the bottom end of the positioning screw is inserted into the top of the top cover. A connecting groove is provided on the top of the top cover, and the positioning screw is inserted into the connecting groove.

[0012] Preferably, the crushing tank is connected to the inside of the feeding tank by bolts, and the crushing tank is located at the top of the feeding tank, the top of the crushing tank is clamped together with the top cover, a driving motor is installed in the middle of the top of the top cover by bolts, and the output end of the driving motor is transmission-connected to the rotating rod, and the end of the rotating rod close to the driving motor is connected to the middle of the top cover through a bearing.

[0013] Preferably, the rotating rod is located in the middle of the crushing tank, and the bottom end of the rotating rod is connected to the arc-shaped grinding plate by bolts, the outer wall of the arc-shaped grinding plate fits the inner wall of the crushing tank, the rotating rod is connected to the crushing knife group by bolts, and the crushing knife group is located above the arc-shaped grinding plate, the crushing tank is connected to the discharge pipe, and an electromagnetic valve is installed on the side of the discharge pipe.

[0014] Preferably, the support seat is connected to the bottom inner wall of the feeding tank by bolts, and the top of the support seat is connected to the feeding pipe by bolts, the end of the feeding pipe away from the support seat passes through the feeding tank, and the feeding pipe is arranged at an inclined angle, the top of one end of the feeding pipe close to the support seat is connected to the discharge pipe, and the feeding pipe and the discharge pipe are connected to each other.

[0015] Preferably, a motor is installed on one side of the feeding tube close to the support seat by bolts, the output end of the motor is transmission-connected to the rotating shaft, and the two ends of the rotating shaft are respectively installed on the two ends of the feeding tube by bearings, the rotating shaft is fixedly connected to the transmission propeller, and the outer wall of the transmission propeller is in contact with the inner wall of the feeding tube.

[0016] Preferably, the feeding pipe is fixedly connected to the discharge pipe, and the discharge pipe is located at the bottom of one end of the feeding pipe away from the support seat, the discharge pipe is slidably connected to the receiving plate, and the outer wall of the receiving plate is in contact with the inside of the discharge pipe, a rotating shaft is provided in the middle of the receiving plate, a weight scale is installed on the bottom of the receiving plate by bolts, a rotating motor is installed on the outer wall of the discharge pipe by bolts, and the output end of the rotating motor is transmission-connected to the rotating shaft in the middle of the receiving plate.

[0017] Compared with the prior art, the utility model has the following beneficial effects:

[0018] 1. The solid salt is crushed by the crushing component, and the solid salt is crushed and ground by the crushing knife group and the arc-shaped grinding plate, which is convenient for subsequent weight detection and feeding, preventing the chemical splash caused by the weight of the block solid salt during the feeding process, and reducing the occurrence of dangerous factors;

[0019] 2. The feeding pipe, rotating shaft and transmission propeller are used to transfer and feed the crushed solid salt, without the need for manual feeding by the staff, which makes the device more convenient to use. The weight of the added material is detected by the set material receiving plate and weight weighing device, thereby improving the accuracy of the experimental data and the experimental efficiency.

[0020] 3. The top cover and the feeding tank are tightly connected by the provided connection assembly to prevent the solid salt inside the crushing tank from overflowing during the crushing process, causing pollution and waste of materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the utility model device;

[0022] Figure 2 It is a schematic diagram of the structure inside the charging tank of the utility model;

[0023] Figure 3 It is a structural schematic diagram of the crushing component of the utility model;

[0024] Figure 4 This utility model Figure 2 The enlarged structural diagram at A in the middle;

[0025] Figure 5 It is a structural schematic diagram of the feeding component of the utility model.

[0026] In the figure: 1. support frame; 2. feeding tank; 3. top cover; 4. connecting assembly; 5. base; 6. rotating seat; 7. rotating rod; 8. connecting plate; 9. positioning screw; 10. crushing assembly; 11. crushing tank; 12. driving motor; 13. rotating rod; 14. crushing knife group; 15. arc grinding plate; 16. discharge pipe; 17. feeding assembly; 18. supporting seat; 19. feeding pipe; 20. motor; 21. rotating shaft; 22. transmission propeller; 23. discharge pipe; 24. control valve; 25. receiving plate; 26. weight scale; 27. rotating motor. DETAILED DESCRIPTION

[0027] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0028] See also Figure 1-Figure 5 As shown, a solid addition device for protein purification and salting out comprises a support frame 1, a feeding tank 2 is arranged on the top of the support frame 1, a top cover 3 is arranged on the top of the feeding tank 2, a crushing component 10 is arranged at the bottom of the top cover 3, a connecting component 4 is arranged on one side of the top of the top cover 3, and a feeding component 17 is arranged inside the feeding tank 2;

[0029] See also Figure 1 , Figure 2 , Figure 4 As shown, the connecting component 4 includes a base 5, a rotating seat 6 is provided on the top of the base 5, and a rotating rod 7 is provided on the top of the rotating seat 6, a connecting plate 8 is provided on the top of the rotating rod 7, and a positioning screw 9 is provided at one end of the connecting plate 8, the side of the base 5 is fixedly connected to the outer wall of the feeding tank 2, and there are three bases 5, which are evenly distributed on the outer wall of the base 5, the top of the base 5 is connected to the rotating seat 6 by bolts, and the rotating seat 6 is rotatably connected to the rotating rod 7, the end of the rotating rod 7 away from the rotating seat 6 is connected to the connecting plate 8 by bolts, and the end of the connecting plate 8 away from the rotating rod 7 is located above the top cover 3, the connecting plate 8 is connected to the positioning screw 9 by threads, and the bottom end of the positioning screw 9 is inserted into the top of the top cover 3, the top of the top cover 3 is provided with a connecting groove, and the positioning screw 9 is inserted into the inside of the connecting groove, and the top cover 3 and the feeding tank 2 are tightly connected by the provided connecting component 4, so as to prevent the solid salt inside the crushing tank 11 from overflowing during the crushing process, causing pollution and waste of materials;

[0030] See also Figure 1 , Figure 2 , Figure 3As shown, the crushing assembly 10 includes a crushing tank 11, a rotating rod 13 is arranged in the middle of the crushing tank 11, an arc-shaped grinding plate 15 is arranged at the bottom of the rotating rod 13, and a crushing knife group 14 is arranged above the arc-shaped grinding plate 15, and a discharge pipe 16 is arranged at the bottom of the crushing tank 11. The crushing tank 11 is connected to the inside of the feeding tank 2 by bolts, and the crushing tank 11 is located at the top of the feeding tank 2. The top of the crushing tank 11 is clamped together with the top cover 3, and a driving motor 12 is installed in the middle of the top of the top cover 3 by bolts, and the output end of the driving motor 12 is transmission-connected to the rotating rod 13, and one end of the rotating rod 13 close to the driving motor 12 is connected to the middle of the top cover 3 through a bearing, and the rotating rod 13 is located in the crushing tank 11. The middle of the rotating rod 13, and the bottom end of the rotating rod 13 is connected to the arc-shaped grinding plate 15 by bolts, the outer wall of the arc-shaped grinding plate 15 is fitted with the inner wall of the crushing tank 11, the rotating rod 13 is connected to the crushing knife group 14 by bolts, and the crushing knife group 14 is located above the arc-shaped grinding plate 15, the crushing tank 11 is connected to the discharge pipe 16, and the side of the discharge pipe 16 is equipped with a solenoid valve, the solid salt is crushed by the provided crushing assembly 10, and the solid salt is crushed and ground by the provided crushing knife group 14 and the arc-shaped grinding plate 15, so as to facilitate the subsequent weight detection and the feeding, and prevent the block solid salt from splashing chemicals due to its own weight during the feeding process, thereby reducing the occurrence of dangerous factors;

[0031] See also Figure 1 , Figure 2 , Figure 5As shown, the feeding assembly 17 includes a support seat 18, a feeding pipe 19 is arranged above the support seat 18, and a rotating shaft 21 is arranged in the middle of the feeding pipe 19, a transmission propeller 22 is arranged on the outer wall of the rotating shaft 21, a discharge pipe 23 is arranged at the bottom of one end of the feeding pipe 19, a control valve 24 is arranged inside one end of the discharge pipe 23, and a receiving plate 25 is arranged inside the other end of the discharge pipe 23, the support seat 18 is connected to the bottom inner wall of the feeding tank 2 by bolts, and the top of the support seat 18 is The feeding pipe 19 is connected to the feeding tank 2 by bolts, and the end of the feeding pipe 19 away from the support seat 18 passes through the feeding tank 2, and the feeding pipe 19 is set at an inclined angle, and the top of the end of the feeding pipe 19 close to the support seat 18 is connected to the discharge pipe 16, and the feeding pipe 19 and the discharge pipe 16 are connected to each other. The side of the feeding pipe 19 close to the support seat 18 is equipped with a motor 20 by bolts, and the output end of the motor 20 is connected to the rotating shaft 21, and the two ends of the rotating shaft 21 are respectively installed on the feeding pipe 19 through bearings. At both ends of the support base 18, the rotating shaft 21 is fixedly connected to the transmission propeller 22, and the outer wall of the transmission propeller 22 is in contact with the inner wall of the feeding pipe 19, the feeding pipe 19 is fixedly connected to the discharge pipe 23, and the discharge pipe 23 is located at the bottom of the end of the feeding pipe 19 away from the support base 18, the discharge pipe 23 is slidably connected to the receiving plate 25, and the outer wall of the receiving plate 25 is in contact with the inside of the discharge pipe 23, a rotating shaft is provided in the middle of the receiving plate 25, and a weight scale 26 is installed at the bottom of the receiving plate 25 by bolts. A rotating motor 27 is installed on the outer wall of the discharge pipe 23 by bolts, and the output end of the rotating motor 27 is connected to the rotating shaft in the middle of the receiving plate 25. The feeding pipe 19, the rotating shaft 21 and the transmission propeller 22 are provided to realize the transmission and feeding of the crushed solid salt. There is no need for manual feeding by the staff, which provides convenience for the use of the device. The receiving plate 25 and the weight scale 26 are provided to detect the weight of the added material, thereby improving the accuracy of the experimental data and improving the experimental efficiency.

[0032] It should be noted that the utility model is a solid adding device for protein purification and salting out. When in use, solid salt is placed inside the crushing tank 11, and then the top cover 3, rotating rod 13, crushing knife group 14 and arc-shaped grinding plate 15 are inserted into the crushing tank 11, and the connecting plate 8 is turned to make the connecting plate 8 drive the rotating rod 7 to rotate along the rotating seat 6, so that the connecting plate 8 rotates to the top of the top cover 3, and the positioning screw 9 is rotated to make the positioning screw 9 inserted into the connecting groove at the top of the top cover 3, so as to achieve a fixed connection between the top cover 3 and the feeding tank 2, and then the rotating rod 13 is driven to rotate by the set driving motor 12, so that the rotating rod 13 drives the crushing knife group 14 and the arc-shaped grinding plate 15 to crush and grind the solid salt, so that the solid salt is broken from block into powder, so as to facilitate subsequent feeding, and the crushing tank 11 is connected to the feeding pipe 19 through the set discharge pipe 16, and the discharge is carried out. An electromagnetic valve is installed inside the tube 16 to control the discharge. After the crushing is completed, the solid salt enters the inside of the feeding tube 19 through the discharge tube 16, and the rotating shaft 21 is driven to rotate by the motor 20. The rotating shaft 21 is fixedly connected to the transmission propeller 22, so that the transmission propeller 22 can realize the transmission of the solid salt entering the inside of the feeding tube 19. The control valve 24 controls the discharge of the discharge tube 23, so that the solid salt falls on the top of the receiving plate 25, and the weight is detected by the weight scale 26 arranged at the bottom of the receiving plate 25. The rotating shaft in the middle of the receiving plate 25 is driven to rotate by the rotating motor 27, so as to realize the flipping of the receiving plate 25 and complete the feeding of the solid salt. A controller is installed on the top of the support frame 1, and the receiving plate 25, the control valve 24, the electromagnetic valve, the rotating motor 27, the motor 20 and the driving motor 12 are all electrically connected to the controller.

[0033] The above shows and describes the basic principle and main features of the utility model and the advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A solid addition device for protein purification and salting out, comprising a support frame (1), characterized in that: A feeding tank (2) is arranged on the top of the support frame (1), and a top cover (3) is arranged on the top of the feeding tank (2), a crushing assembly (10) is arranged on the bottom of the top cover (3), and a connecting assembly (4) is arranged on one side of the top of the top cover (3), and a feeding assembly (17) is arranged inside the feeding tank (2); The connecting assembly (4) comprises a base (5), a rotating seat (6) is arranged on the top of the base (5), a rotating rod (7) is arranged on the top of the rotating seat (6), a connecting plate (8) is arranged on the top of the rotating rod (7), and a positioning screw (9) is arranged at one end of the connecting plate (8); The crushing assembly (10) comprises a crushing tank (11), a rotating rod (13) is arranged in the middle of the crushing tank (11), an arc-shaped grinding plate (15) is arranged at the bottom of the rotating rod (13), a crushing knife group (14) is arranged above the arc-shaped grinding plate (15), and a discharge pipe (16) is arranged at the bottom of the crushing tank (11); The feeding assembly (17) comprises a support seat (18), a feeding pipe (19) is arranged above the support seat (18), a rotating shaft (21) is arranged in the middle of the feeding pipe (19), a transmission propeller (22) is arranged on the outer wall of the rotating shaft (21), a discharge pipe (23) is arranged at the bottom of one end of the feeding pipe (19), a control valve (24) is arranged inside one end of the discharge pipe (23), and a receiving plate (25) is arranged inside the other end of the discharge pipe (23).

2. The solid addition device for protein purification and salting out according to claim 1, characterized in that: The side of the base (5) is fixedly connected to the outer wall of the feeding tank (2), and there are three bases (5) which are evenly distributed on the outer wall of the base (5). The top of the base (5) is connected to the rotating base (6) by bolts, and the rotating base (6) is rotatably connected to the rotating rod (7).

3. The solid addition device for protein purification and salting out according to claim 2, characterized in that: The end of the rotating rod (7) away from the rotating seat (6) is connected to the connecting plate (8) by a bolt, and the end of the connecting plate (8) away from the rotating rod (7) is located above the top cover (3). The connecting plate (8) is connected to the positioning screw (9) by a thread, and the bottom end of the positioning screw (9) is inserted into the top of the top cover (3). The top of the top cover (3) is provided with a connecting groove, and the positioning screw (9) is inserted into the inside of the connecting groove.

4. The solid addition device for protein purification and salting out according to claim 1, characterized in that: The crushing tank (11) is connected to the inside of the feeding tank (2) by bolts, and the crushing tank (11) is located at the top of the feeding tank (2). The top of the crushing tank (11) is clamped together with the top cover (3). A driving motor (12) is installed in the middle of the top of the top cover (3) by bolts, and the output end of the driving motor (12) is transmission-connected to the rotating rod (13). One end of the rotating rod (13) close to the driving motor (12) is connected to the middle of the top cover (3) by a bearing.

5. The solid addition device for protein purification and salting out according to claim 4, characterized in that: The rotating rod (13) is located in the middle of the crushing tank (11), and the bottom end of the rotating rod (13) is connected to the arc-shaped grinding plate (15) by bolts, the outer wall of the arc-shaped grinding plate (15) is in contact with the inner wall of the crushing tank (11), the rotating rod (13) is connected to the crushing knife group (14) by bolts, and the crushing knife group (14) is located above the arc-shaped grinding plate (15), the crushing tank (11) is connected to the discharge pipe (16), and a solenoid valve is installed on the side of the discharge pipe (16).

6. The solid addition device for protein purification and salting out according to claim 1, characterized in that: The support seat (18) is connected to the bottom inner wall of the feeding tank (2) by bolts, and the top of the support seat (18) is connected to the feeding pipe (19) by bolts, the end of the feeding pipe (19) away from the support seat (18) passes through the feeding tank (2), and the feeding pipe (19) is arranged at an inclined angle, the top of one end of the feeding pipe (19) close to the support seat (18) is connected to the discharge pipe (16), and the feeding pipe (19) and the discharge pipe (16) are connected to each other.

7. The solid addition device for protein purification and salting out according to claim 6, characterized in that: A motor (20) is installed on one side of the feeding pipe (19) close to the support seat (18) by means of bolts, the output end of the motor (20) is transmission-connected to a rotating shaft (21), and the two ends of the rotating shaft (21) are respectively installed on the two ends of the feeding pipe (19) by means of bearings, the rotating shaft (21) is fixedly connected to a transmission propeller (22), and the outer wall of the transmission propeller (22) is in contact with the inner wall of the feeding pipe (19).

8. The solid addition device for protein purification and salting out according to claim 7, characterized in that: The feeding pipe (19) is fixedly connected to the discharge pipe (23), and the discharge pipe (23) is located at the bottom of one end of the feeding pipe (19) away from the support seat (18). The discharge pipe (23) is slidably connected to the receiving plate (25), and the outer wall of the receiving plate (25) is in contact with the inside of the discharge pipe (23). A rotating shaft is arranged in the middle of the receiving plate (25), and a weight scale (26) is installed at the bottom of the receiving plate (25) by bolts. A rotating motor (27) is installed on the outer wall of the discharge pipe (23) by bolts, and the output end of the rotating motor (27) is drivingly connected to the rotating shaft in the middle of the receiving plate (25).

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