Sleeve filtering device for separating and purifying cell exosome at normal temperature

Abstract

The utility model relates to the technical field of cell exosomes, in particular to a casing pipe filtering device for separating and purifying cell exosomes at normal temperature, which comprises a pipe body and a pipe cap, a filtering structure is arranged on the inner wall of the pipe body, the filtering structure comprises a circular ring, the circular ring is in sliding connection with the inner wall of the pipe body, one side of the circular ring is communicated with a filtering pipe, and the other side of the circular ring is communicated with the pipe cap. One end of the filter pipe is fixedly connected with a plurality of pieces of filter paper, the arc surface of the circular ring is fixedly connected with a plurality of sealing rings, the sealing rings are rubber rings, the sealing rings are of hollow structures, the inner wall of the filter pipe is fixedly connected with convex blocks, the convex blocks are arc-shaped blocks, and the side, away from the filter pipe, of the circular ring is fixedly connected with two handles. A plurality of anti-skid grooves are formed in the surface of the handle, one end of the filter tube is conical, and the filter paper is fixedly connected to the conical surface. According to the utility model, the problem that the loss of more samples is possibly caused by the use of whole piece type filter paper in the existing sleeve is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of extracellular vesicles, and in particular to a sleeve filtering device for separating and purifying extracellular vesicles at room temperature. Background Art

[0002] Exosomes are small vesicles secreted by cells, which contain a variety of biological molecules, such as proteins, nucleic acids, lipids, etc. When processing exosomes, they are separated from the cell culture supernatant through centrifugation and filtration techniques, and then a series of purification steps are performed to obtain pure exosome samples.

[0003] Existing related technologies often have the following defects: In order to prevent contamination and protect samples when using cannulas for separation and purification of exosomes at room temperature, the existing cannulas use a whole piece of filter paper, which may cause a lot of sample loss, especially for samples with tiny particles, such as exosomes, the whole piece of filter paper may prevent part of the sample from passing through, resulting in sample loss.

[0004] Therefore, the utility model provides a sleeve filter device for separating and purifying cell exosomes at room temperature. Utility Model Content

[0005] The utility model aims to solve the shortcoming in the prior art that the existing cannula uses a whole piece of filter paper which may cause more sample loss, and proposes a cannula filtering device for separating and purifying cell exosomes at room temperature.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a sleeve filter device for separating and purifying cell exosomes at room temperature, comprising a tube body and a tube cap, the inner wall of the tube body is provided with a filtering structure, the filtering structure comprises a circular ring, the circular ring is slidably connected to the inner wall of the filter, one side of the circular ring is connected to a filter tube, and one end of the filter tube is fixedly connected to a plurality of filter papers.

[0007] The effect achieved by the above components is: by setting up a filtering structure, using multiple pieces of filter paper for filtering to improve the filtration of exosomes, and avoiding the situation where the filter paper is blocked after filtering too much and affecting the filtration effect when the exosomes are filtered using a whole piece of filter paper.

[0008] Preferably, a plurality of sealing rings are fixedly connected to the arc surface of the circular ring, and the sealing rings are rubber rings.

[0009] The effect achieved by the above components is: the circular ring will drive the sealing ring to slide closely along the inner wall of the tube body, and the sealing ring will deform with the help of the squeezing force, so that the sealing ring can improve the airtightness of the fit between the circular ring and the inner wall of the tube body, thereby preventing unfiltered exosomes from flowing into the tube body through the gap between the circular ring and the inner wall of the tube body, thereby improving the filtering effect on exosomes.

[0010] Preferably, the sealing ring is a hollow structure.

[0011] The effect achieved by the above components is that the sealing ring can deform more easily and adapt to the unevenness of the sealing surface when deforming.

[0012] Preferably, a protrusion is fixedly connected to the inner wall of the filter tube, and the protrusion is an arc-shaped block.

[0013] The effect achieved by the above components is: the arc-shaped block prevents the exosomes from accumulating in the filter tube, thereby improving the filtering effect on the exosomes.

[0014] Preferably, two handles are fixedly connected to one side of the circular ring away from the filter tube, and a plurality of anti-slip grooves are formed on the surface of the handles.

[0015] The effect achieved by the above components is that the movement of the handle will drive the ring to move, and the handle can facilitate the movement of the ring.

[0016] Preferably, one end of the filter tube is conical, and the filter paper is fixedly connected to the conical surface.

[0017] The effect achieved by the above components is: it is easy to filter out the exosomes from the filter tube, thereby further improving the filtering effect on the exosomes.

[0018] In summary:

[0019] 1. In the utility model, by setting a filtering structure, the filter tube is placed in the tube body, the ring will fit in the tube body, and then the exosomes are poured into the filter tube. During filtration, the exosomes will pass through the filter paper, thereby filtering the exosomes, avoiding the situation where the filter paper is blocked after filtering too much and affecting the filtration effect when the exosomes are filtered using a whole piece of filter paper. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0021] Figure 2 This is a schematic diagram of the disassembled structure of the utility model;

[0022] Figure 3 This is a schematic diagram of the structure of the filter tube of the utility model;

[0023] Figure 4 It is a schematic diagram of the cross-sectional structure of the sealing ring of the utility model;

[0024] Figure 5 It is a structural schematic diagram of the arc block of the utility model.

[0025] Legend: 1. Tube body; 2. Tube cap; 3. Filter structure; 31. Ring; 32. Filter tube; 33. Filter paper; 34. Sealing ring; 35. Bump; 36. Handle; 37. Anti-slip groove. DETAILED DESCRIPTION

[0026] Reference Figure 1 As shown, the utility model provides a technical solution: a sleeve filter device for separating and purifying cell exosomes at room temperature, comprising a tube body 1 and a tube cap 2, the inner wall of the tube body 1 is provided with a filtering structure 3, by setting the filtering structure 3, using a multi-piece filter paper 33 for filtering to improve the filtration of exosomes, and avoiding the situation where the filter paper 33 is blocked after filtering too much and affecting the filtration effect when the exosomes are filtered using a whole piece of filter paper 33.

[0027] Reference Figure 2 - Figure 5 As shown, in this embodiment: the filtering structure 3 includes a ring 31, the ring 31 is slidably connected to the inner wall of the filter, one side of the ring 31 is connected to a filter tube 32, and one end of the filter tube 32 is fixedly connected to a plurality of filter papers 33. The arc surface of the ring 31 is fixedly connected to a plurality of sealing rings 34, which are rubber rings. The ring 31 will drive the sealing ring 34 to slide closely along the inner wall of the tube body 1. The sealing ring 34 will deform with the help of the squeezing force. The sealing ring 34 can improve the tightness of the fit between the ring 31 and the inner wall of the tube body 1, thereby preventing the unfiltered exosomes from flowing into the tube body 1 through the gap between the ring 31 and the inner wall of the tube body 1, thereby improving the filtering effect on the exosomes. The sealing ring 34 is a hollow structure, and the sealing ring 34 can be more easily deformed and adapted to the unevenness of the sealing surface when deformed. The inner wall of the filter tube 32 is fixedly connected with a protrusion 35, which is an arc-shaped block. The arc-shaped block can prevent exosomes from accumulating in the filter tube 32, thereby improving the filtering effect on the exosomes. Two handles 36 are fixedly connected to the side of the ring 31 away from the filter tube 32. The surface of the handle 36 is provided with a plurality of anti-slip grooves 37. The movement of the handle 36 will drive the movement of the ring 31, and the handle 36 can facilitate the movement of the ring 31. One end of the filter tube 32 is conical, and the filter paper 33 is fixedly connected to the conical surface, so as to facilitate the filtering of the exosomes from the filter tube 32, thereby further improving the filtering effect on the exosomes.

[0028] Working principle: when it is necessary to filter exosomes, first pinch the two handles 36. The movement of the handles 36 will drive the ring 31 to move, thereby facilitating the movement of the ring 31. The movement of the ring 31 will drive the filter tube 32 to move, and the filter tube 32 will be placed in the tube body 1. The filter tube 32 will drive the ring 31 to slide along the inner wall of the tube body 1, and the ring 31 will drive the sealing ring 34 to slide tightly along the inner wall of the tube body 1. The sealing ring 34 will deform with the help of the squeezing force, and the sealing ring 34 can improve the airtightness of the fit between the ring 31 and the inner wall of the tube body 1, thereby preventing unfiltered exosomes from flowing into the tube body 1 through the gap between the ring 31 and the inner wall of the tube body 1, thereby improving the filtering effect of the exosomes. Because the sealing ring 34 is a hollow structure, the sealing ring 34 can be more easily deformed and adapt to the unevenness of the sealing surface when deformed. Then the exosomes are poured into the filter tube 32. During filtration, the exosomes will pass through the filter paper 33, thereby filtering the exosomes.

[0029] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

Claims

1. A sleeve filter device for separating and purifying cell exosomes at room temperature, comprising a tube body (1) and a tube cap (2), wherein the inner wall of the tube body (1) is provided with a filtering structure (3), characterized in that: The filtering structure (3) comprises a circular ring (31), the circular ring (31) being slidably connected to the inner wall of the filter, one side of the circular ring (31) being connected to a filter tube (32), and one end of the filter tube (32) being fixedly connected to a plurality of filter papers (33).

2. A cannula filtration device for separating and purifying exosomes at room temperature according to claim 1, characterized in that: A plurality of sealing rings (34) are fixedly connected to the arc surface of the circular ring (31), and the sealing rings (34) are rubber rings.

3. A cannula filtration device for separating and purifying exosomes at room temperature according to claim 2, characterized in that: The sealing ring (34) is a hollow structure.

4. The cannula filtration device for separating and purifying exosomes at room temperature according to claim 1, characterized in that: A protrusion (35) is fixedly connected to the inner wall of the filter tube (32), and the protrusion (35) is an arc-shaped block.

5. The cannula filtration device for separating and purifying exosomes at room temperature according to claim 1, characterized in that: Two handles (36) are fixedly connected to one side of the circular ring (31) away from the filter tube, and a plurality of anti-slip grooves (37) are provided on the surface of the handles (36).

6. The cannula filtration device for separating and purifying exosomes at room temperature according to claim 1, characterized in that: One end of the filter tube (32) is conical, and the filter paper (33) is fixedly connected to the conical surface.

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