Automatic control device for efficient cracking of excrement DNA (deoxyribonucleic acid) extracting solution

Abstract

The utility model discloses an automatic control device for efficient cracking of a feces DNA (deoxyribonucleic acid) extracting solution. The automatic control device for efficient cracking of the excrement DNA extracting solution comprises a support, and a cracking mechanism and a control end are installed on the support; the cracking mechanism comprises two cracking tanks, shells of the cracking tanks are connected, a temperature sensor and a heating coil are mounted in each cracking tank, a gear ring is mounted at the top end of each cracking tank, a plurality of stirring shafts are mounted at the bottom of each gear ring in an annular array manner, a gear is mounted on one side of each gear ring in a meshed manner, and a driving motor is mounted above each gear. According to the automatic control device for efficient cracking of the excrement DNA extracting solution, the bracket, the cracking mechanism and the control end are arranged, the control end is used for controlling the temperature in the cracking tanks, and the stirring shaft can be controlled to stir the cracking solution, smash a sample and accelerate the cracking speed of the sample; the time required for cracking can be effectively shortened, and the detection efficiency can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cracking equipment, in particular to an automatic control device for efficient cracking of feces DNA extracting liquid. Background Art

[0002] When conducting rapid nucleic acid detection of Echinococcus eggs in dog feces, direct microscopic examination, saturated salt water flotation, or saturated sucrose solution flotation is generally used to screen fresh dog feces from the selected site. First, a dog feces sample needs to be taken, a lysis solution is added, and the sample is placed in a constant temperature heater for a certain period of time, and then it can be added to the reaction system as a DNA template.

[0003] However, after adding the lysis solution, the existing dog feces samples need to be lysed in a constant temperature heater for about 5 minutes, which is not efficient enough. For the detection of large sample quantities, such as more than 200 samples, it takes a lot of time.

[0004] Therefore, it is necessary to provide an automatic control device for efficient lysis of fecal DNA extract to solve the above technical problems. Utility Model Content

[0005] In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a feces DNA extraction liquid efficient lysis automatic control device which can accelerate the sample lysis speed and improve the detection efficiency.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0007] The invention discloses an automatic control device for efficient lysis of fecal DNA extract, comprising: a bracket, on which a lysis mechanism and a control end are installed, the lysis mechanism comprises a lysis tank with two shells connected, a temperature sensor and a heating coil are installed in the lysis tank, the temperature sensor and the heating coil are connected to the control end, a gear ring is rotatably installed on the top of the lysis tank, a plurality of stirring shafts are installed in a circular array at the bottom of the gear ring, a gear is meshingly installed on one side of the gear ring, a driving motor is installed above the gear, the shell of the driving motor is fixedly connected to the shell of the lysis tank, and the driving motor is connected to the control end.

[0008] Preferably, a slag discharge pipe is installed at the bottom of the cracking tank, a liquid discharge pipe is installed at the side of the cracking tank, and valves are installed on the liquid discharge pipe and the slag discharge pipe.

[0009] Preferably, a feed pipe is installed on the top of the lysis tank, and the feed pipe is used as an inlet for the sample and the lysis solution at the same time. The gear ring is rotatably connected to the feed pipe through a bearing, and a cover is installed on the feed pipe.

[0010] Preferably, a seal is installed in the cracking tank, and the seal is arranged below the gear ring.

[0011] Preferably, the sealing member comprises a fixing ring and a sealing ring mounted on the top of the fixing ring, the outer side of the fixing ring is fixedly connected to the inner wall of the cracking tank, and the top of the sealing ring contacts the bottom of the gear ring.

[0012] Compared with the prior art, the utility model has the following beneficial effects:

[0013] (1) The utility model provides a support, a lysis mechanism and a control end, uses the control end to control the temperature in the lysis tank, and can control the stirring shaft to stir the lysis solution, break up the sample, and accelerate the lysis speed of the sample. The two lysis tanks are provided at the same time, which can effectively reduce the time required for lysis, thereby facilitating the improvement of detection efficiency;

[0014] (2) The utility model installs a liquid discharge pipe and a slag discharge pipe on the cracking tank to discharge the liquid used for detection separately, which is more convenient for detection;

[0015] (3) The utility model provides a feeding pipe installed on the top of the lysis tank, which can facilitate the feeding of samples and lysis solutions into the lysis tank and support the gear ring;

[0016] (4) The utility model can prevent the lubricating grease on the gear ring from falling into the pyrolysis tank and contaminating the sample by installing a seal under the gear ring;

[0017] (5) The utility model can effectively seal the lower part of the gear ring by providing a sealing member including a fixing ring and a sealing ring. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a front view structural schematic diagram of the automatic control device for efficient lysis of fecal DNA extraction solution provided by the utility model;

[0019] Figure 2 for Figure 1 The schematic diagram of the structure of the lysis mechanism in the automatic control device for efficient lysis of fecal DNA extraction solution is shown;

[0020] Figure 3 for Figure 1 The schematic diagram of the structure of the seal in the automatic control device for efficient lysis of fecal DNA extraction liquid is shown.

[0021] Among them, the names corresponding to the figure marks are: 1-bracket, 2-cracking mechanism, 3-control end, 4-cracking tank, 5-temperature sensor, 6-heating coil, 7-stirring shaft, 8-drain pipe, 9-slag discharge pipe, 10-feed pipe, 11-gear ring, 12-gear, 13-drive motor, 14-seal, 15-fixing ring, 16-seal ring. DETAILED DESCRIPTION

[0022] The present invention is further described below in conjunction with the accompanying drawings and embodiments. The present invention includes but is not limited to the following embodiments.

[0023] Embodiment 1:

[0024] like Figure 1-3 As shown, the feces DNA extracting liquid efficient lysis automatic control device provided by the utility model comprises: a bracket 1, a lysis mechanism 2 and a control end 3 are installed on the bracket 1, the lysis mechanism 2 comprises a lysis tank 4 with two shells connected, a temperature sensor 5 and a heating coil 6 are installed in the lysis tank 4, the temperature sensor 5 and the heating coil 6 are connected to the control end 3, a gear ring 11 is rotatably installed on the top of the lysis tank 4, a plurality of stirring shafts 7 are installed in a circular array at the bottom of the gear ring 11, a gear 12 is meshingly installed on one side of the gear ring 11, a driving motor 13 is installed above the gear 12, the shell of the driving motor 13 is fixedly connected to the shell of the lysis tank 4, the driving motor 13 is connected to the control end 3, when in use, first the control end 3 controls the heating coil 6 to energize and heat the lysis tank 4, so that the lysis tank 4 is heated, the temperature sensor 5 detects the temperature of the lysis tank 4, and transmits the temperature data to the temperature sensor 5. The value is transmitted to the control end 3. After the temperature of the lysis tank 4 reaches the preset lysis temperature (the temperature is generally set at about 95°C), the control end 3 automatically controls the heating coil 6 to cut off the power, and after the temperature is lower than the set temperature, the heating coil 6 will be energized again, and a certain amount of lysis solution is injected into the lysis tank 4, and then a certain amount of canine feces sample is put into the lysis tank 4, and then the drive motor 13 can be started through the control end 3, and the gear ring 11 is driven to rotate through the gear 12, thereby driving the stirring shaft 7 to rotate, breaking up the sample, making the tissue cells in the sample more dispersed, and accelerating the lysis speed of the sample. Compared with the conventional method, it can effectively reduce the time required for lysis, thereby improving the detection efficiency, and is suitable for large-scale sample detection. In addition, the device is provided with two lysis tanks 4, which can be used for two samples at the same time, and can further improve the detection efficiency.

[0025] By setting up a bracket 1, a lysis mechanism 2 and a control end 3, the control end 3 is used to control the temperature in the lysis tank 4, and the stirring shaft 7 can be controlled to stir the lysis liquid, break up the sample, and accelerate the lysis speed of the sample. At the same time, two lysis tanks 4 are set up, which can effectively reduce the time required for lysis, thereby helping to improve the detection efficiency.

[0026] Embodiment 2:

[0027] like Figure 2 As shown, a slag discharge pipe 9 is installed at the bottom of the cracking tank 4, a liquid discharge pipe 8 is installed on the side of the cracking tank 4, and valves are installed on the liquid discharge pipe 8 and the slag discharge pipe 9. After the sample is cracked in the cracking tank 4, the liquid used for detection is discharged through the liquid discharge pipe 8, and the large particles and the remaining liquid are discharged through the slag discharge pipe 9.

[0028] By installing a liquid discharge pipe 8 and a slag discharge pipe 9 on the cracking tank 4, the liquid used for detection can be discharged separately, which makes detection more convenient.

[0029] Embodiment 3:

[0030] like Figure 2 As shown, a feed pipe 10 is installed on the top of the lysis tank 4. The feed pipe 10 is used as an inlet for samples and lysis solutions. The gear ring 11 is rotatably connected to the feed pipe 10 through a bearing. A cover is installed on the feed pipe 10. When in use, samples and lysis solutions are added into the lysis tank 4 through the feed pipe 10, and then the cover is closed to close the mouth of the feed pipe 10. Similarly, after the lysis tank 4 is used, clean water is injected into the lysis tank 4 through the feed pipe 10 to clean the inside of the lysis tank 4.

[0031] By installing the feeding tube 10 on the top of the lysis tank 4 , it is convenient to put the sample and the lysis solution into the lysis tank 4 , and it is also possible to support the gear ring 11 .

[0032] Embodiment 4:

[0033] like Figure 2 As shown, a seal 14 is installed in the cracking tank 4, and the seal 14 is arranged below the gear ring 11. When in use, the seal 14 is used to seal the gear ring 11 to prevent the lubricating grease on the gear ring 11 from falling into the cracking tank 4, contaminating the sample and affecting the accuracy of the test result.

[0034] By installing a seal 14 under the gear ring 11 , it is possible to prevent the lubricating grease on the gear ring 11 from falling into the lysis tank 4 and causing contamination to the sample.

[0035] Embodiment 5:

[0036] like Figure 3 As shown, the seal 14 includes a fixed ring 15 and a sealing ring 16 installed on the top of the fixed ring 15. The outer side of the fixed ring 15 is fixedly connected to the inner wall of the cracking tank 4, and the top of the sealing ring 16 contacts the bottom of the gear ring 11. When in use, the lubricating grease on the gear ring 11 and the debris generated during the meshing rotation fall on the top of the fixed ring 15, and the inner side is blocked by the sealing ring 15, so that the lubricating grease and the debris cannot fall into the cracking tank 4.

[0037] By providing the sealing member 14 including the fixing ring 15 and the sealing ring 16 , the lower part of the ring gear 11 can be effectively sealed.

[0038] Working principle: when in use, first the control end 3 controls the heating coil 6 to energize and heat the lysis tank 4, so that the lysis tank 4 heats up, the temperature sensor 5 detects the temperature of the lysis tank 4, and transmits the temperature value to the control end 3, after the temperature of the lysis tank 4 reaches the preset lysis temperature, the control end 3 automatically controls the heating coil 6 to cut off the power, and after the temperature is lower than the set temperature, it will energize the heating coil 6 again, and repeat the temperature control of the lysis tank 4 to make it reach a constant temperature, and then inject a certain amount of lysis liquid into the lysis tank 4, and then put a certain amount of canine feces sample into the lysis tank 4, and then the drive motor 13 can be started through the control end 3, and the gear 12 drives the gear ring 11 to rotate, thereby driving the stirring shaft 7 to rotate, breaking up the sample, making the tissue cells in the sample more dispersed, and accelerating the lysis speed of the sample. Compared with the conventional method, it can effectively reduce the time required for lysis, thereby improving the detection efficiency, and is suitable for large-scale sample detection. In addition, the device is provided with two lysis tanks 4, which can be used for two samples at the same time, and can further improve the detection efficiency.

Claims

1. An automatic control device for efficient lysis of fecal DNA extract, characterized in that: include: A bracket (1), on which a cracking mechanism (2) and a control end (3) are mounted; The cracking mechanism (2) comprises a cracking tank (4) with two shells connected to each other, a temperature sensor (5) and a heating coil (6) being installed in the cracking tank (4), a gear ring (11) being installed at the top of the cracking tank (4), a plurality of stirring shafts (7) being installed in a ring array at the bottom of the gear ring (11), a gear (12) being meshed and installed on one side of the gear ring (11), a driving motor (13) being installed above the gear (12), and the driving motor (13), the temperature sensor (5) and the heating coil (6) are all connected to the control end (3).

2. The automatic control device for efficient lysis of fecal DNA extract according to claim 1, characterized in that: A slag discharge pipe (9) is installed at the bottom of the cracking tank (4).

3. The automatic control device for efficient lysis of fecal DNA extract according to claim 1, characterized in that: A liquid discharge pipe (8) is installed on the side of the cracking tank (4).

4. The automatic control device for efficient lysis of fecal DNA extract according to claim 1, characterized in that: A feed pipe (10) is installed on the top of the cracking tank (4), and the gear ring (11) is connected to the feed pipe (10).

5. The automatic control device for efficient lysis of fecal DNA extract according to claim 1, characterized in that: A sealing member (14) is installed in the cracking tank (4), and the sealing member (14) is arranged below the gear ring (11).

6. The automatic control device for efficient lysis of fecal DNA extract according to claim 5, characterized in that: The sealing member (14) comprises a fixing ring (15) and a sealing ring (16).

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