Multifunctional microscopic imaging analyzer

Abstract

The utility model relates to a multifunctional microscopic imaging analyzer which comprises a base, an object carrying assembly, a light source assembly, an imaging assembly and a light path adjusting assembly, the light source assembly comprises a fluorescent assembly, a white light assembly and a coherent light assembly; the imaging assembly comprises a fluorescence camera used for capturing an image obtained through fluorescence multichannel imaging; the bright field camera is used for capturing images obtained by white light bright field imaging and quantitative phase imaging; along a light path from a sample to the imaging assembly, the light path adjusting assembly sequentially comprises an objective lens, a first right-angle mirror, a tube mirror, a second right-angle mirror and a first beam splitter prism cube. According to the multifunctional microscopic imaging analyzer disclosed by the utility model, by optimizing the light source configuration and the light path design, a highly integrated imaging system is realized, so that a user can complete various imaging requirements on one device, the cost is saved, and the use convenience and the experiment flexibility are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of microscopic optical imaging, in particular to a multifunctional microscopic imaging analyzer. Background Art

[0002] Microscopy is one of the most important tools in biomedical research and clinical diagnosis. To observe the structure, function, and dynamic changes of cells and tissues, researchers and clinicians rely on a variety of microscopy imaging methods, including white-field imaging, fluorescence multi-channel imaging, and quantitative phase imaging (QPI).

[0003] White light bright field imaging is the traditional and most commonly used imaging technique, but its imaging effect is limited for transparent or low-contrast samples. Fluorescence imaging can provide detailed information about specific biomarkers, but requires additional fluorescent probes and excitation light sources. Quantitative phase imaging technology provides quantitative information of the sample and is suitable for label-free real-time cell imaging, but lacks the specificity of fluorescence imaging technology.

[0004] Current devices on the market tend to focus on a single imaging mode, which requires researchers to use and switch between multiple microscopes when conducting experiments with multiple imaging techniques, which is not only inefficient but also costly. Utility Model Content

[0005] The purpose of the utility model is to provide a multifunctional microscopic imaging analyzer to solve the problem that existing equipment often focuses on a single imaging mode, and researchers need to use and switch multiple microscopes when conducting experiments with multiple imaging techniques, resulting in inefficiency and high costs.

[0006] To achieve this purpose, the utility model adopts the following technical solutions:

[0007] A multifunctional microscopic imaging analyzer, comprising:

[0008] A base, used to carry various components;

[0009] A loading assembly, used to support and position the sample to be observed;

[0010] The light source assembly includes: a fluorescent assembly for providing light within a specific wavelength range to excite fluorescent markers in the sample; a white light assembly for providing light with a wide spectrum for white light bright field imaging; and a coherent light assembly for providing a coherent light source required for phase imaging;

[0011] The imaging component includes: a fluorescence camera for capturing images obtained by fluorescence multi-channel imaging; a bright field camera for capturing images obtained by white light bright field imaging and quantitative phase imaging;

[0012] And an optical path adjustment component, along the optical path from the sample to the imaging component, the optical path adjustment component sequentially includes: an objective lens, a first right-angle mirror, a tube lens, a second right-angle mirror and a first beam splitter cube.

[0013] Optionally, polarizing lenses are provided between the second right-angle mirror and the first beam splitter cube, and between the first beam splitter cube and the bright field camera, respectively.

[0014] Optionally, the fluorescent component includes:

[0015] A fluorescent light source generator for generating light within a plurality of specific wavelength ranges;

[0016] A plurality of fluorescent cubes, each of which corresponds to light within a specific wavelength range;

[0017] and a moving component, on which the fluorescent light source generator and the fluorescent cube are both arranged, and the moving component is used to move the corresponding fluorescent cube to a position between the objective lens and the first right-angle mirror according to imaging requirements.

[0018] Optionally, the white light assembly includes: a column fixed to the base, a beam fixed to the column, and a second beam splitter prism cube arranged on the beam;

[0019] A first white light source is disposed on the second beam splitter cube, and a second white light source is disposed under the crossbeam;

[0020] The first white light source, the second beam splitter cube, the second white light source, the objective lens and the first right-angle mirror are arranged along the same axis;

[0021] The first white light source, the second beam splitter cube and the second white light source are arranged on one side of the sample, and the objective lens and the first right-angle mirror are arranged on the other side of the sample.

[0022] Optionally, the first white light source is a cylindrical light source, and the second white light source is a ring-shaped light source.

[0023] Optionally, along the optical path of the coherent light, the coherent light component includes in sequence: a laser, a coupling lens, a first fiber coupler, a collimating lens, and a third right-angle mirror;

[0024] The first fiber coupler is used to guide a laser light source to the collimating lens, and the third right-angle mirror is used to reflect the laser light source to the first beam splitter cube;

[0025] The coherent optical assembly further includes a second optical fiber coupler, which is fixed to the second beam splitter cube and is used to guide another laser light source to the second beam splitter cube.

[0026] Optionally, the multifunctional microscopic imaging analyzer further comprises an automatic lifting component for adjusting the height of the objective lens, and the automatic lifting component comprises:

[0027] A support, fixed to the base;

[0028] A slide mounting plate connected to the support via fine-tuning screws;

[0029] An automatic linear slide, fixed to the slide mounting plate;

[0030] and a supporting member connecting the slider of the automatic linear slide and the objective lens.

[0031] Optionally, the multifunctional microscopic imaging analyzer further includes an electric turntable, and the objective lens is arranged on the support member via the electric turntable.

[0032] Compared with the prior art, the utility model has the following beneficial effects:

[0033] The fluorescence component, white light component and coherent light component provide three light sources to meet different imaging needs. The multifunctional microscopic imaging analyzer provided in the present application can realize seamless switching between white light bright field imaging, fluorescence multi-channel imaging and quantitative phase imaging, and these three imaging modes share a set of optical path adjustment components. The bright field camera can be used to capture images obtained by white light bright field imaging and quantitative phase imaging. By optimizing the light source configuration and optical path design, a highly integrated imaging system is realized. This integrated design not only reduces the cost and dependence on multiple dedicated equipment, but also greatly improves the research efficiency; especially when facing the same observation sample, the user can easily switch between different imaging modes to obtain more comprehensive information without changing the camera or making complex configuration adjustments. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0035] The structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with this technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantive technical significance. Any structural modification, change in proportion or adjustment of size, without affecting the effects and purposes that can be achieved by the present invention, should still fall within the scope of the technical contents disclosed by the present invention.

[0036] Figure 1 Schematic diagram of the optical path structure of the multifunctional microscopic imaging analyzer in this embodiment;

[0037] Figure 2 Schematic diagram of the structure of the multifunctional microscopic imaging analyzer in this embodiment;

[0038] Figure 3 is a schematic diagram of the structure of the fluorescent component in this embodiment;

[0039] Figure 4 is a schematic diagram of the structure of the white light component in this embodiment;

[0040] Figure 5 is a schematic diagram of the structure of the coherent optical component in this embodiment;

[0041] Figure 6 Schematic diagram of the structure of the automatic lifting component in this embodiment.

[0042] Description of reference numerals:

[0043] 10. Base;

[0044] 20. Carrying components;

[0045] 31. Fluorescent component; 311. Fluorescent light source generator; 312. Fluorescent cube; 313. Moving component;

[0046] 32. white light assembly; 321. column; 322. beam; 323. second beam splitter prism cube; 324. first white light source; 325. second white light source;

[0047] 33. Coherent optical component; 331. Laser; 332. Coupling lens; 333. First fiber coupler; 334. Collimating lens; 335. Third right-angle mirror; 336. Second fiber coupler;

[0048] 41. Fluorescence camera; 42. Bright field camera;

[0049] 51. Objective lens; 52. First right-angle mirror; 53. Tube lens; 54. Second right-angle mirror; 55. First beam splitter prism cube; 56. Polarizing lens;

[0050] 60. Automatic lifting assembly; 61. Support; 62. Slide mounting plate; 63. Automatic linear slide; 64. Support member;

[0051] 70. Electric turntable. DETAILED DESCRIPTION

[0052] In order to make the utility model's purpose, features, and advantages more obvious and easy to understand, the following will be combined with the drawings in the utility model embodiments to clearly and completely describe the technical solutions in the utility model embodiments. Obviously, the embodiments described below are only part of the utility model embodiments, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0053] In the description of the present invention, it should be understood that the terms "upper", "lower", "top", "bottom", "inside", "outside", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. It should be noted that when a component is considered to be "connected" to another component, it may be directly connected to the other component or there may be a centrally arranged component at the same time.

[0054] The technical solution of the utility model is further explained below with reference to the accompanying drawings and through specific implementation methods.

[0055] Reference Figures 1 to 6 The multifunctional microscopic imaging analyzer disclosed in this embodiment includes: a base 10 for carrying various components, a loading component 20 (an automatic loading platform) for supporting and positioning the sample to be observed, a light source component, an imaging component, and an optical path adjustment component;

[0056] The light source assembly includes: a fluorescent assembly 31, which is used to provide light within a specific wavelength range to excite fluorescent markers in the sample; a white light assembly 32, which is used to provide a wide spectrum of light for white light bright field imaging; a coherent light assembly 33, which is used to provide a coherent light source required for phase imaging;

[0057] The imaging assembly includes: a fluorescence camera 41 for capturing images obtained by fluorescence multi-channel imaging; a bright field camera 42 for capturing images obtained by white light bright field imaging and quantitative phase imaging;

[0058] Along the optical path from the sample to the imaging component, the optical path adjustment component includes in sequence: an objective lens 51 (for collecting light emitted or reflected by the sample), a first right-angle mirror 52 (for adjusting the direction of the optical path), a tube lens 53 (for further adjusting and guiding the light beam), a second right-angle mirror 54 (for further adjusting the direction of the optical path to match the requirements of different imaging modes) and a first beam splitter cube 55 (for selectively separating or recombining light of different wavelengths according to imaging requirements and transmitting them to the fluorescence camera 41 or the bright field camera 42).

[0059] The fluorescent component 31, the white light component 32 and the coherent light component 33 provide three light sources to meet different imaging needs. The multifunctional microscopic imaging analyzer provided in the present application can achieve seamless switching between white light bright field imaging, fluorescent multi-channel imaging and quantitative phase imaging, and these three imaging modes share a set of optical path adjustment components. The bright field camera 42 can be used to capture images obtained by white light bright field imaging and quantitative phase imaging. By optimizing the light source configuration and optical path design, a highly integrated imaging system is achieved. This integrated design not only reduces the cost and dependence on multiple dedicated equipment, but also greatly improves research efficiency. Especially when facing the same observation sample, users can easily switch between different imaging modes to obtain more comprehensive information without changing the camera or making complex configuration adjustments.

[0060] Reference Figure 1 Furthermore, polarizing lenses 56 are provided between the second right-angle mirror 54 and the first beam splitter cube 55, and between the first beam splitter cube 55 and the bright field camera 42. This helps to improve the imaging contrast, especially for the observation of transparent or low-contrast samples.

[0061] The beam splitter cube is mainly composed of two right-angled triangular prisms bonded together. The bonding interface is usually coated with a special optical coating. Through the specific coating, the beam splitter cube can select a specific wavelength of light for transmission and reflect other wavelengths of light.

[0062] Fluorescence Cube 312 is a key optical component in microscope imaging systems, especially in applications involving fluorescence imaging. It is a cubic structure composed of excitation filters, emission filters and dichroic mirrors, which is used to accurately control the light path through the microscope to ensure that only light of specific wavelengths is allowed to pass, thereby achieving efficient fluorescence excitation and fluorescence signal collection.

[0063] It should be noted that the above-mentioned objective lens 51, first right-angle mirror 52, tube lens 53, second right-angle mirror 54, first beam splitter cube 55, polarizing lens 56 and the fluorescent light source generator 311, fluorescent cube 312 and other optical devices mentioned below are all prior arts and are well known to those skilled in the art. Therefore, the structure and principle of each optical device will not be described in detail here. In addition, those skilled in the art can infer the angle setting of each device based on the name, position relationship, connection relationship and other information of each device disclosed in this application. Therefore, the angle setting of each device will not be described in detail here.

[0064] Reference Figure 3 , wherein the fluorescent component 31 includes: a fluorescent light source generator 311 (a wide-spectrum LED light source or a laser light source), which is used to generate light within a plurality of specific wavelength ranges; a plurality of fluorescent cubes 312, each fluorescent cube 312 corresponds to light within a specific wavelength range (ultraviolet light, blue light, green light, red light, etc.); and a moving component 313, on which the fluorescent light source generator 311 and the fluorescent cube 312 are both arranged, and the moving component 313 is used to move the corresponding fluorescent cube 312 to a position between the objective lens 51 and the first right-angle mirror 52 according to imaging requirements (when using white light bright field imaging and quantitative phase imaging functions, the moving component 313 moves the fluorescent cube 312 to an empty state).

[0065] Each fluorescence cube 312 corresponds to different fluorescence excitation and emission filtering requirements. The fluorescence light source generator 311 is combined with multiple fluorescence cubes 312 to generate and select light within a specific wavelength range as needed to meet the needs of multi-channel fluorescence imaging. The fluorescence cube 312 can be easily switched through the mobile component 313, making the switching and positioning of the light source and the fluorescence cube 312 simple and fast, greatly improving the experimental efficiency and conversion speed.

[0066] Reference Figure 4 Further, the white light assembly 32 includes: a column 321 fixed to the base 10, a beam 322 fixed to the column 321, and a second beam splitter prism cube 323 arranged on the beam 322; a first white light source 324 is arranged on the second beam splitter prism cube 323, and a second white light source 325 is arranged under the beam 322; the first white light source 324, the second beam splitter prism cube 323, the second white light source 325, the objective lens 51 and the first right-angle mirror 52 are arranged along the same axis; wherein the first white light source 324, the second beam splitter prism cube 323 and the second white light source 325 are arranged on one side of the sample, and the objective lens 51 and the first right-angle mirror 52 are arranged on the other side of the sample.

[0067] The first white light source 324 (through the cage plate) is disposed on the second beam splitter cube 323 to provide the main illumination source. The light of the first white light source 324 is guided toward the sample through the second beam splitter cube 323. The second white light source 325 is disposed under the crossbeam 322 to serve as an auxiliary light source to supplement the light of the first white light source 324 or provide illumination at different angles to improve the imaging quality. The combination of the first white light source 324 and the second white light source 325 ensures highly flexible and wide-ranging illumination requirements, thereby enabling the microscopic imaging analyzer to adapt to a wide range of observation and imaging applications.

[0068] The first white light source 324 is a cylindrical light source, providing direct illumination; the second white light source 325 is an annular light source, located below the crossbeam 322, providing uniform annular illumination to improve the imaging quality of the sample.

[0069] Reference Figure 1 and Figure 5 Furthermore, along the optical path of the coherent light, the coherent optical component 33 includes in sequence: a laser 331, a coupling lens 332, a first fiber coupler 333, a collimating lens 334 and a third right-angle mirror 335; the first fiber coupler 333 is used to guide a laser light source to the collimating lens 334, and the third right-angle mirror 335 is used to reflect the laser light source to the first beam splitter prism cube 55; the coherent optical component 33 also includes a second fiber coupler 336, and the second fiber coupler 336 is fixed to the second beam splitter prism cube 323 (through a cage translation adjustment frame) and is used to guide another laser light source to the second beam splitter prism cube 323.

[0070] The laser 331 and its subsequent optical path accessories combination (coupling lens 332, first fiber coupler 333, etc.) provide the required coherent light source for quantitative phase imaging (QPI), allowing label-free real-time cell imaging with higher image quality.

[0071] Reference Figure 6 Furthermore, the multifunctional microscopic imaging analyzer also includes an automatic lifting component 60 for adjusting the height of the objective lens 51, and the automatic lifting component 60 includes: a support 61, which is fixed to the base 10; a slide mounting plate 62, which is connected to the support 61 through fine-tuning screws; an automatic linear slide 63, which is fixed to the slide mounting plate 62; and a support member 64, which connects the slider of the automatic linear slide 63 and the objective lens 51.

[0072] The automatic lifting assembly 60 realizes accurate automatic adjustment of the height of the objective lens 51, so that the user can conveniently adjust the focal length and quickly obtain clear imaging, which improves the convenience and efficiency of operation during the experiment.

[0073] Furthermore, the multifunctional microscopic imaging analyzer also includes an electric turntable 70 , and the objective lens 51 is disposed on the support 64 via the electric turntable 70 .

[0074] The objective lens 51 is combined with the support 64 through the electric turntable 70, making the switching of the objective lens 51 more convenient, accurate and rapid, allowing the user to quickly select objective lenses 51 of different magnifications through electric control, thereby adapting to different observation and imaging requirements, and significantly improving the use flexibility and operational convenience of the microscope imaging analyzer.

[0075] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multifunctional microscopic imaging analyzer, characterized in that: include: A base (10) for carrying various components; A loading assembly (20), used for supporting and positioning a sample to be observed; The light source assembly comprises: a fluorescent assembly (31) for providing light within a specific wavelength range to excite fluorescent markers in a sample; a white light assembly (32) for providing light with a wide spectrum for white light bright field imaging; and a coherent light assembly (33) for providing a coherent light source required for phase imaging; The imaging component comprises: a fluorescence camera (41) for capturing images obtained by fluorescence multi-channel imaging; a bright field camera (42) for capturing images obtained by white light bright field imaging and quantitative phase imaging; and an optical path adjustment component, which, along the optical path from the sample to the imaging component, comprises in sequence: an objective lens (51), a first right-angle mirror (52), a tube lens (53), a second right-angle mirror (54), and a first beam splitter prism cube (55).

2. The multifunctional microscopic imaging analyzer according to claim 1, characterized in that: Polarizing lenses (56) are respectively provided between the second right-angle mirror (54) and the first beam splitting prism cube (55), and between the first beam splitting prism cube (55) and the bright field camera (42).

3. The multifunctional microscopic imaging analyzer according to claim 1, characterized in that: The fluorescent component (31) comprises: A fluorescent light source generator (311) for generating light within a plurality of specific wavelength ranges; A plurality of fluorescent cubes (312), each fluorescent cube (312) corresponding to light within a specific wavelength range; and a moving component (313), the fluorescent light source generator (311) and the fluorescent cube (312) are both arranged on the moving component (313), and the moving component (313) is used to move the corresponding fluorescent cube (312) to a position between the objective lens (51) and the first right-angle mirror (52) according to imaging requirements.

4. The multifunctional microscopic imaging analyzer according to claim 1, characterized in that: The white light assembly (32) comprises: a column (321) fixed to the base (10), a crossbeam (322) fixed to the column (321), and a second beam splitter prism cube (323) arranged on the crossbeam (322); A first white light source (324) is provided on the second beam splitter cube (323), and a second white light source (325) is provided under the crossbeam (322); The first white light source (324), the second beam splitting prism cube (323), the second white light source (325), the objective lens (51), and the first right-angle mirror (52) are arranged along the same axis; The first white light source (324), the second beam splitter cube (323) and the second white light source (325) are arranged on one side of the sample, and the objective lens (51) and the first right-angle mirror (52) are arranged on the other side of the sample.

5. The multifunctional microscopic imaging analyzer according to claim 4, characterized in that: The first white light source (324) is a cylindrical light source, and the second white light source (325) is an annular light source.

6. The multifunctional microscopic imaging analyzer according to claim 4, characterized in that: Along the optical path of the coherent light, the coherent light component (33) comprises in sequence: a laser (331), a coupling lens (332), a first optical fiber coupler (333), a collimating lens (334), and a third right-angle mirror (335); The first optical fiber coupler (333) is used to guide a beam of laser light source to the collimating lens (334), and the third right-angle mirror (335) is used to reflect the laser light source to the first beam splitting prism cube (55); The coherent optical component (33) further comprises a second optical fiber coupler (336), wherein the second optical fiber coupler (336) is fixed to the second beam splitter prism cube (323) and is used to guide another beam of laser light source to the second beam splitter prism cube (323).

7. The multifunctional microscopic imaging analyzer according to claim 1, characterized in that: It also includes an automatic lifting component (60) for adjusting the height of the objective lens (51), the automatic lifting component (60) comprising: A support (61) fixed to the base (10); A slide mounting plate (62) connected to the support (61) via fine-tuning screws; An automatic linear slide (63) fixed to the slide mounting plate (62); and a support member (64) connecting a slider of the automatic linear slide table (63) and the objective lens (51).

8. The multifunctional microscopic imaging analyzer according to claim 7, characterized in that: It also includes an electric turntable (70), and the objective lens (51) is arranged on the support member (64) via the electric turntable (70).

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