Controllable phenotype equipment for collecting phenotypes of seeds in vertical germination period
By designing a controllable phenotypic device including a vertical seed germination plate and a movable camera robot arm, the problem of difficulty in capturing phenotypic information during the vertical germination of seeds is solved in the prior art, and efficient and accurate phenotypic information collection is achieved, which is suitable for modern mechanized agricultural production and plant phenotypic technology.
Patent Information
- Application Number
- CN202421363489.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-15
- Publication Date
- 2025-03-14
- Estimated Expiration
- 2034-06-15
AI Technical Summary
The prior art is difficult to accurately capture phenotypic information during the vertical germination period of seeds, especially the growth data of underground root systems. Traditional devices mainly adapt to the horizontal germination state and cannot meet the requirements of crop phenolics.
A controllable phenotype device including a vertical seed germination plate placement rack, a drip irrigation system, a vertical seed germination plate and a movable camera robot arm are designed. Vertical seed germination plates provide space for seeds to germinate in situ, drip irrigation system ensures water supply, and movable camera robotic arms are used to efficiently collect phenotypic information during the vertical seed germination period.
It has achieved efficient and accurate collection of phenotypic information during the vertical germination period of seeds, overcome the problems of inaccurate data acquisition and lack of dynamic tracking capabilities in traditional methods, and is suitable for dynamic, high-throughput, and lossless acquisition of seed germination phenotype images in plant phenolics technology.
Smart Images
Figure CN222602995U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of seed germination period phenotype collection, in particular to a controllable phenotype device for collecting seed vertical germination period phenotypes. Background Art
[0002] Seeds are the starting point of agricultural production, and seed germination is the most important link in the growth process of crops. Exploring the relationship between seed germination phenotype, crop genotype and environmental factors, and optimizing crop genes and external environment can not only significantly improve the quality of seedlings and better adapt to modern mechanized agricultural production, but also ensure national food security.
[0003] The phenotypic traits of seed germination mainly include seed length, seed width, germination rate, germination potential, seedling length, root growth rate, maximum root length and other parameters. The traditional method of collecting crop information based on drones and other devices equipped with various optical lenses is difficult to capture underground root growth data, and the data collection is often not accurate enough, with limited monitoring accuracy and lack of dynamic tracking capabilities; in addition, the current seed germination period phenotypic collection devices are mainly based on the germination state of seeds, which is quite different from a wide range of field conditions and cannot meet the requirements of crop phenotyping.
[0004] Therefore, how to provide a controllable phenotyping device for collecting the phenotypes of seeds during the vertical germination period has become a technical problem that technicians in this field urgently need to solve. Utility Model Content
[0005] The purpose of the utility model is to provide a controllable phenotyping device for collecting the phenotype of the vertical germination period of seeds in view of the deficiencies in the prior art. The specially made vertical seed germination plate of the device provides a space for the seeds to germinate in situ, while the drip irrigation system ensures the water supply required during the seed germination process. The seed germination plate display rack is flexible and stackable. A large number of vertical seed germination plates can be set up according to demand. Combined with a movable mechanical arm, it can efficiently collect phenotypic information of large-scale seeds during the vertical germination period.
[0006] To achieve the above-mentioned purpose, the utility model provides a controllable phenotyping device for collecting the phenotype of seeds in the vertical germination period, comprising: a vertical seed germination plate display rack, a drip irrigation pipe, a vertical seed germination plate and a movable camera mechanical arm; a plurality of bearing cross bars are installed in the middle and upper part of the vertical seed germination plate display rack, and a punching plate is laid between the bearing cross bars, the vertical seed germination plate is connected to the punching plate, and the liquid outlet end of the drip irrigation pipe extends into the vertical seed germination plate; the movable camera mechanical arm is located on one side of the vertical seed germination plate display rack, and is used to collect the phenotypic information of the seeds in the vertical germination period.
[0007] Furthermore, a plurality of the vertical seed germination plates may be hung on each punching plate.
[0008] Furthermore, the upper part of the vertical seed germination plate has an oblique structure for placing seeds, which can adapt to seeds of different sizes and keep the seeds growing in situ. The oblique angle and size include but are not limited to other forms of germination plates.
[0009] Furthermore, each of the vertical seed germination plates is provided with seed germination filter paper for absorbing water and maintaining a moist environment for the seeds. The seeds are placed at the oblique position of the seed germination plate to ensure observation of the germination process.
[0010] Furthermore, circular holes are provided on both sides of the top of each vertical seed germination plate, and hooks are connected to the circular holes, and the seed germination plate is connected to the punching plate through the hooks.
[0011] Furthermore, the styles of the germination board include not only a transparent hollow structured integrated germination board, but also a detachable non-integrated germination board.
[0012] Furthermore, a fixed aluminum profile is installed at the bottom end of the vertical seed germination plate display rack, and both sides of the top end of the fixed aluminum profile are also connected to the vertical seed germination plate display rack through supporting aluminum profiles.
[0013] Furthermore, the movable camera mechanical arm includes a movable base, a mechanical arm body, a replaceable camera module and a mechanical arm picking clamp; the mechanical arm body is installed on the top of the movable base, and a plurality of universal wheels are installed on the bottom of the movable base; the replaceable camera module is fixed to the top of the mechanical arm body by a buckle, and the mechanical arm picking clamp is fixed to the top of the mechanical arm body by a knob.
[0014] Furthermore, a loading platform is provided on one side of the movable base.
[0015] Furthermore, it also includes a drip irrigation system, which is connected to the drip irrigation pipe.
[0016] The beneficial effects of the utility model are:
[0017] The utility model provides a controllable phenotyping device for efficiently collecting the phenotypes of seeds during the vertical germination period. The seeds are placed in vertical seed germination plates respectively, and the plates are placed on a vertical seed germination plate display rack. The drip irrigation system ensures the supply of water during the seed germination period, and the movable camera mechanical arm is used to simultaneously collect the phenotypic information of the seeds during the vertical germination period. The oblique structure of the special vertical seed germination plate and the seed germination filter paper fix the germinating seeds, solving the problem of the lack of the carrier required in the dynamic detection of seed in situ germination. In addition, the utility model can further keep each vertical seed germination plate in a vertical position through the setting of a vertical seed germination plate display rack, so that the radicle and plumule can grow and develop under natural conditions during the seed germination process. The vertical seed germination plates do not interfere with each other, and also provide convenience for image analysis for the post-processing of seed phenotypic images during the germination period and the extraction of seed germination morphological parameters. The movable camera mechanical arm selected in the utility model can realize the automatic taking and placing of the vertical seed germination plate through the mechanical arm picking clamp and the connecting parts on the vertical seed germination plate. It has a high degree of mechanization and reduces the input of human resources. It is suitable for the dynamic, high-throughput and non-destructive collection of seed germination phenotype images under the background of plant phenotypic genomics technology. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a schematic diagram of the structure of the utility model;
[0019] Figure 2 This is a schematic diagram of the structure of the movable camera mechanical arm of the utility model;
[0020] Figure 3 This is a schematic diagram of the connection structure between the drip irrigation system and the germination plate of the utility model;
[0021] Figure 4 It is a side view of a vertical germination board in the utility model;
[0022] Figure 5 It is a three-dimensional structural schematic diagram of another vertical germination board in the utility model;
[0023] Figure 6 It is a rear view of another vertical germination board in the utility model;
[0024] Figure 7 This is a diagram showing the results of extracting dynamic root length information during the germination period of wheat seeds in the utility model.
[0025] Among them, in the figure:
[0026] 1-vertical seed germination board display rack; 2-vertical seed germination board; 3-punching plate; 4-bearing cross bar; 5-supporting aluminum profile; 6-fixed aluminum profile; 7-connecting base aluminum profile; 8-replaceable camera module; 9-mechanical arm body; 10-base aluminum profile; 11-movable base; 12-carrying platform; 13-universal wheel; 14-mechanical arm object picking clamp; 15-round hole; 16-hook; 17-drip irrigation pipe; 18-drip irrigation system; 19-connecting ring; 20-seeds to be germinated; 21-oblique mouth; 22-germination filter paper; 23-detachable back plate. DETAILED DESCRIPTION
[0027] The technical solutions in the embodiments of the utility model are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0028] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequential order. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of the present application described here. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device comprising a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.
[0029] In the present application, the terms "upper", "lower", "left", "right", "front", "back", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings. These terms are mainly used to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to have a specific orientation, or to be constructed and operated in a specific orientation.
[0030] In addition, some of the above terms may be used to express other meanings in addition to indicating orientation or positional relationship. For example, the term "on" may also be used to express a certain dependency or connection relationship in some cases. For those of ordinary skill in the art, the specific meanings of these terms in this application can be understood according to specific circumstances.
[0031] In addition, the terms "installed", "set", "provided with", "connected", "connected", and "socketed" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection, or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be an internal connection between two devices, elements, or components. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.
[0032] like Figure 1-4 As shown, the utility model provides a controllable phenotyping device for collecting the phenotype of seeds in the vertical germination period, comprising: a vertical seed germination plate display rack 1, a drip irrigation pipe 18, a vertical seed germination plate 2 and a movable camera mechanical arm; a plurality of bearing cross bars 4 are installed in the middle and upper part of the vertical seed germination plate display rack 1, the vertical seed germination plate 3 is connected to the punching plate 3 between the bearing cross bars 4, and the liquid outlet end of the drip irrigation pipe 17 extends into the vertical seed germination plate 2; the movable camera mechanical arm is located on one side of the vertical seed germination plate display rack 1, and is used to collect the phenotypic information of the seeds in the vertical germination period.
[0033] The vertical seed germination plate 2 is a transparent hard shell plate with a hollow window, and the internal oblique structure 21 and seed germination filter paper 22 provide in-situ root growth space for crops. In this embodiment, four bearing cross bars 4 are provided to carry the punching plate 3 carrying the vertical germination plate 2, so that a large number of seed germination period phenotypes can be collected at the same time.
[0034] For further optimization, a plurality of the vertical seed germination plates 2 can be hung on each perforated plate 3 according to experimental requirements, so as to achieve synchronous processing of different experimental batches or different seeds.
[0035] Further optimization, a seed germination filter paper 22 is placed inside each of the vertical seed germination plates 2, and the seeds 20 to be germinated are placed in the gap between the seed germination filter paper 21 and the oblique mouth 21 of the vertical seed germination plate. The number of seeds and the placement interval can be determined according to experimental needs, seed size and fibrous root system / tap root system.
[0036] Further optimization, circular holes 15 are opened on both sides of the top of each vertical seed germination plate 2, and hooks 16 are connected to the circular holes 15, which are connected to the punching plate 3 through the hooks 16 to ensure that the seeds can maintain a vertical germination state during germination.
[0037] Further optimization, the bevel angle and size of the vertical seed germination plate are flexibly set, including but not limited to an integrated vertical seed germination plate, and also includes a detachable non-integrated vertical seed germination plate, such as Figure 5 and Figure 6 As shown, a removable back panel 23 is used.
[0038] Further optimization, a fixed aluminum profile 6 is installed at the bottom end of the vertical seed germination plate display rack 1, and both sides of the top of the fixed aluminum profile 6 are connected to the vertical seed germination plate display rack 1 through supporting aluminum profiles 5, and the bottom of the vertical seed germination plate display rack 1 is supported by a connecting aluminum profile 7 and a base aluminum profile 10.
[0039] Further optimization, the movable camera arm includes a replaceable camera module 8, a robot body 9 and a robot arm object picking clamp 14; the robot body 9 is installed on the top of the movable base 11, and a plurality of universal wheels 13 are installed on the bottom of the movable base 11; the replaceable camera module 8 is fixed to the top of the robot body by a buckle, and the robot arm object picking clamp 14 is fixed to the top of the robot body by a knob. The movable camera arm is equipped with a replaceable camera module, and with the movement of the robot body, a large amount of seed germination period phenotypes can be collected in a short time. At the same time, with the robot arm object picking clamp, it can automatically place the vertical seed germination plate and collect phenotypic data. In this embodiment, six universal wheels 13 are provided to facilitate the movement of the movable camera arm.
[0040] For further optimization, a loading platform 12 is provided on one side of the movable base 11 .
[0041] The utility model further comprises a drip irrigation system 18 , which is connected to the drip irrigation pipe 17 , and the drip irrigation pipes are connected via a connecting ring 19 .
[0042] The utility model provides a growth and observation space for crop roots through a specially made vertical seed germination plate, solving the problem that roots in the soil are difficult to observe in traditional phenotypic collection. The drip irrigation system provides the required water for seed germination in a timely manner, and the overall equipment realizes real-time monitoring and high-quality image acquisition of in-situ growth phenotypes during the seed germination period. In addition, the vertical seed germination plate display rack of the utility model is flexible and stackable. A large number of vertical seed germination plates can be set up according to demand, and combined with a movable camera arm, a large amount of seed germination period image data can be collected simultaneously.
[0043] like Figure 7 As shown, Figure 7 This is a result diagram of the dynamic root length information extraction of wheat seeds during the germination period. By collecting dynamic RGB images of the seed germination period, the ImageJ and OpenCV libraries can be used to manually and automatically extract the phenotypic information of the ROI area during the seed germination period. The utility model provides a flexible image information acquisition method to meet the diverse needs of the laboratory for seed germination period phenotypic extraction.
[0044] The utility model provides a controllable phenotyping device for collecting the phenotype of seeds during the vertical germination period. The seeds are placed in vertical seed germination plates respectively, which are placed on a vertical seed germination plate display rack, and the phenotypic information of the seeds during the vertical germination period is collected simultaneously by using a movable camera mechanical arm. The utility model utilizes the oblique design of a special vertical seed germination plate and seed germination filter paper to fix the germinating seeds, thereby overcoming the problem of the lack of carriers required for dynamic phenotypic detection during the vertical germination of seeds. In addition, the utility model can further maintain the vertical placement of each vertical seed germination plate through the provision of a vertical seed germination plate display rack, so that the radicle and plumule can grow and develop under natural conditions during the seed germination process. The vertical seed germination plates do not interfere with each other, and also provide convenience for image analysis for the post-processing of seed phenotypic images during the germination period and the extraction of seed germination morphological parameters. The movable camera mechanical arm selected in the utility model can realize the automatic taking and placing of the vertical seed germination plate through the mechanical arm picking clamp and the connecting parts on the vertical seed germination plate. It has a high degree of mechanization and reduces the input of human resources. It is suitable for the dynamic, high-throughput and non-destructive collection of seed germination phenotype images under the background of plant phenotypic genomics technology.
[0045] Specifically, when collecting phenotypic information of seeds during germination using the above-mentioned controllable phenotyping device, the following six steps may be included:
[0046] Step 1: Preparation: Select the corresponding seed germination filter paper according to the type of experimental seeds, and place the selected filter paper into the sterilized vertical seed germination plate.
[0047] Step 2: Seed placement. Adjust the number and spacing of seeds placed on the germination filter paper according to the type and quantity of seeds. Place the sterilized seeds in the gap between the oblique opening of the vertical seed germination plate and the seed germination filter paper, and place them neatly in order.
[0048] Step 3: Adjust the water supply. Slowly pour an appropriate amount of pure water along the wall of the germination plate to ensure that the water is evenly distributed. Adjust the position and angle of the drip irrigation pipe to meet the water demand during the seed germination period.
[0049] Step 4: Install the germination board. Connect the vertical seed germination board containing the seeds to be tested to the hooks on the display rack through the two round holes on its upper part. Fix it on the punching plate of the vertical seed germination board display rack according to the shooting requirements, and adjust the connection angle between the germination board, hook and display rack to make it stable.
[0050] Step 5: Set up the camera arm. Select a suitable replaceable camera module and fix it on the top of the arm. Set the movement trajectory of the arm, the time and frequency of image acquisition, and the number of vertical seed germination plates for which data needs to be collected. Start the movable arm and make it run automatically according to the preset trajectory until the set shooting task is completed.
[0051] Step 6: Data extraction. Select appropriate image processing methods based on the quality of the captured images and the characteristics of the seed variety. Use ImageJ to manually extract phenotypic information, or call the OpenCV library to automatically extract phenotypic information of the ROI area during the seed germination period.
[0052] Tables 1-4 below show the root length data of different types of seeds during the germination period obtained by acquiring images using the patented device and extracting them using different methods.
[0053] Table 1 Root length data of corn seeds during germination period
[0054]
[0055]
[0056] Table 2 Root length data of rapeseed seeds during germination period
[0057]
[0058] Table 3 Root length data of cotton seeds during germination period
[0059]
[0060]
[0061] Table 4 Root length data of wheat seeds during germination period
[0062]
[0063] In Tables 1-4, except for the root length data of corn seeds in Table 1, which was manually extracted using ImageJ, the data in other tables were batch extracted by the OpenCV library. The four types of seeds include taproot seeds and fibrous root seeds, indicating that the utility model patent can meet the requirements of capturing and extracting dynamic in situ phenotypic information of different seed germination periods.
[0064] The above description is only a preferred embodiment of the present invention and does not limit the technical scope of the present invention. Therefore, any minor modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are still within the scope of the technical solution of the present invention.
Claims
1. A controllable phenotyping device for collecting vertical germination period phenotypes of seeds, characterized in that: include: A vertical seed germination plate display rack (1), wherein a plurality of bearing cross bars (4) are installed in the middle and upper part of the display rack, and punching plates (3) are laid between the bearing cross bars; A vertical seed germination plate (2), the vertical seed germination plate being connected to the punching plate; A drip irrigation system, the drip irrigation system being fixed to a vertical seed germination plate placement rack, and the liquid outlet end of the drip irrigation pipe (17) extending into the vertical seed germination plate; A movable camera mechanical arm is located on one side of the vertical seed germination plate display rack and is used to collect phenotypic information of seeds during the vertical germination period.
2. A controllable phenotyping device for collecting vertical germination period phenotypes of seeds as claimed in claim 1, characterized in that: A plurality of the vertical seed germination plates (2) are hung on each punching plate (3).
3. A controllable phenotyping device for collecting vertical germination period phenotypes of seeds as claimed in claim 1, characterized in that: Each of the vertical seed germination plates (2) is provided with seed germination filter paper (22) for absorbing water and maintaining a moist environment for the seeds. The seeds are placed at the oblique opening (21) of the seed germination plate to ensure observation of the germination process.
4. A controllable phenotyping device for collecting vertical germination period phenotypes of seeds as claimed in claim 3, characterized in that: Circular holes (15) are provided on both sides of the top of each vertical seed germination plate (2), and hooks (16) are connected to the circular holes (15). The vertical seed germination plate (2) is connected to the punching plate (3) via the hooks (16).
5. A controllable phenotyping device for collecting vertical germination period phenotypes of seeds as claimed in claim 1, characterized in that: A fixed aluminum profile (6) is installed at the bottom end of the vertical seed germination plate display rack (1), and both sides of the top end of the fixed aluminum profile (6) are also connected to the vertical seed germination plate display rack (1) via supporting aluminum profiles (5).
6. A controllable phenotyping device for collecting vertical germination period phenotypes of seeds as claimed in claim 1, characterized in that: The movable camera mechanical arm comprises a replaceable camera module (8), a mechanical arm body (9), a movable base (11) and a mechanical arm object-picking clamp (14); the mechanical arm body (9) is mounted on the top of the movable base (11), and a plurality of universal wheels (13) are mounted on the bottom of the movable base (11); the replaceable camera module (8) is fixed to the top of the mechanical arm body by a buckle, and the mechanical arm object-picking clamp (14) is fixed to the top of the mechanical arm body by a knob.
7. A controllable phenotyping device for collecting vertical germination period phenotypes of seeds as claimed in claim 6, characterized in that: A loading platform (12) is provided on one side of the movable base (11).
8. A controllable phenotyping device for collecting vertical germination period phenotypes of seeds as claimed in claim 1, characterized in that: It also includes a drip irrigation system (18), wherein the drip irrigation system (18) is connected to the drip irrigation pipe (17), and the drip irrigation pipes are connected via a connecting ring (19).