Distributed cooling assembly and cryopreservation tube thereof

Abstract

The utility model relates to the technical field of cryopreservation tubes, and discloses a distributed cooling assembly and a cryopreservation tube thereof, the distributed cooling assembly comprises a fixing part used for controlling the position of an immune cell container and a sealing plate adjusting plate used for achieving the sealing purpose, the fixing part comprises a cryopreservation box, the cavity of the cryopreservation box is in a hollow state, and the sealing plate adjusting plate is arranged in the cryopreservation box. The sealing plate adjusting plate comprises a connecting plate, a cavity of the connecting plate is hollow, a sealing plate is fixedly installed on the wall face of the bottom of the connecting plate, and a clamping device used for sealing the cryopreservation box is arranged in the cavity of the connecting plate. According to the scheme, the volatilization pipe is arranged and penetrates through the connecting plate and the sealing plate, due to the fact that the cryopreservation box cavity is filled with nitrogen liquid, the nitrogen liquid has certain volatility, nitrogen is formed after the nitrogen liquid is volatilized, the nitrogen is discharged outwards through the volatilization pipe, then nitrogen liquid is injected into the cryopreservation box cavity through the nitrogen liquid tank, and it is guaranteed that the air pressure in the cryopreservation box cavity is stable.

Description

Technical Field

[0001] The utility model belongs to the technical field of cryopreservation tubes, and in particular relates to a distributed cooling component and a cryopreservation tube thereof. Background Art

[0002] The prior art discloses an immune cell freezing device (authorization announcement number CN 216853592 U), including a device body, a convenient pick-up and placement device is arranged on the top of the device body, four freezing grooves are opened on the top of the device body, freezing tubes are arranged on the inner wall of the freezing grooves, and auxiliary blocks are fixedly connected to the top of the freezing tubes. The immune cell freezing device is provided with a convenient pick-up and placement device, and under the action of the threaded rod and the second threaded barrel, the connecting plate can rotate with the second threaded barrel, and then the freezing tubes to be used can be selectively clamped. When clamping, the positioning block on the U-shaped frame is used to cooperate with the positioning hole on the auxiliary block, and then the second threaded barrel is limited in position with the second limiting rod, so that the second threaded barrel can move along the threaded rod, and then the freezing tube can be taken out of the freezing groove, without direct contact with the freezing tube and the device body, so as to avoid frostbite of the palm by low temperature.

[0003] 1. The above technology does not achieve sealing of the body, which may cause the temperature inside the body to be replaced with the atmospheric temperature during the cryopreservation of immune cells, thereby affecting the cryopreservation efficiency of immune cells;

[0004] 2. The above technology only has four freezing tanks, and the number of frozen immune cells is small, which is only suitable for small-scale experiments and cannot meet the large-scale batch freezing of immune cells. Its use is greatly limited;

[0005] 3. The above technology uses a large number of mechanical devices, including threaded rods, etc., and its mechanical parts must be driven by motors. During the freezing process, the immune cells will transmit a certain amount of cold air, and when the cold air comes into contact with the air, a certain amount of water vapor will be generated. The water vapor will have a certain impact on the motor, causing the motor to fail to work normally. If the motor fails to work, the purpose of switching the device cannot be achieved.

[0006] In view of this, the present utility model is proposed. Utility Model Content

[0007] In order to solve the above technical problems, the basic concept of the technical solution adopted by the utility model is:

[0008] A distributed cooling component includes a fixed part for controlling the position of an immune cell container and a sealing plate adjustment plate for achieving sealing purposes, the fixed part includes a freezing box, the freezing box cavity is hollowed out, the sealing plate adjustment plate includes a connecting plate, the connecting plate cavity is hollowed out, a sealing plate is fixedly installed on the bottom wall of the connecting plate, a clamping device for sealing the freezing box is arranged in the connecting plate cavity, the clamping device includes a limiting rod for connecting the connecting plate to the freezing box and a moving block for controlling the movement of the limiting rod; a sealing groove is provided on the top wall of the freezing box, the sealing groove is T-shaped, and connecting grooves are provided on the inner walls on both sides of the sealing groove; there are two limiting rods, both of which are movably installed in the connecting plate cavity, and the position of the limiting rod corresponds to the position of the connecting groove.

[0009] As a preferred embodiment of the utility model, there are two connecting grooves, the two connecting grooves are in parallel, the position of the sealing groove corresponds to the position of the connecting plate, the limit rod is movably installed in the corresponding connecting groove cavity, the limit rods all penetrate the connecting plate, and a movable rod is fixedly installed on the top wall of one end of the limit rod extending into the connecting plate cavity.

[0010] As a preferred embodiment of the utility model, a movable groove is opened on the top wall surface of the connecting plate, the position of the movable groove corresponds to the position of the movable rod, the movable rod is movably installed in the movable groove cavity, the movable rods all penetrate the corresponding movable grooves, and movable blocks are fixedly installed on the top wall surface of the movable rod, and the movable blocks are movably installed on the top wall surface of the connecting plate, and a spring is fixedly installed on the outer wall of one end of the limit rod extending into the connecting plate cavity, and the other end of the spring is fixedly connected to the inner wall of the connecting plate.

[0011] As a preferred embodiment of the utility model, a volatilization tube is fixedly installed on the top wall of the connecting plate for volatilizing and discharging the nitrogen liquid in the freezing box cavity. The volatilization tube is L-shaped, and passes through the corresponding connecting plate and the sealing plate and extends into the corresponding freezing box cavity.

[0012] As a preferred embodiment of the utility model, a nitrogen liquid pipe is fixedly installed on one side wall of the freezing box, the nitrogen liquid pipe runs through the freezing box, a valve is fixedly installed on one end of the nitrogen liquid pipe away from the freezing box, and a nitrogen liquid tank for replenishing nitrogen liquid in the freezing box cavity is provided at the other end of the valve. A fixed plate is fixedly installed in the freezing box cavity, and a limiting hole is provided on the top wall of the fixed plate. There are a plurality of limiting holes, and the limiting holes are distributed in a rectangular array on the surface of the fixed plate.

[0013] A freezing tube includes the above-mentioned distributed cooling component, a tube body is movably installed in the limiting hole cavity, there are several tube bodies, and a tube cap is movably installed on the top wall of the tube body, and the tube body and the tube cap are threadedly connected; scales are provided on the outer wall of the tube body, and the scales are evenly distributed on the outer wall of the corresponding tube body, and porcelain beads are provided in the tube body cavity; there are several porcelain beads, and adsorption holes are opened on the outer wall of the porcelain beads.

[0014] Compared with the prior art, the utility model has the following beneficial effects:

[0015] 1. This scheme arranges a snap-fit ​​device, which is arranged in the cavity of the connecting plate. The connecting plate and the freezing box are connected by the snap-fit ​​device. At the same time, the sealing plate arranged at the bottom of the connecting plate is inserted into the cavity of the freezing box to seal the top of the freezing box, thereby achieving the purpose of sealing the freezing box, preventing the freezing of immune cells from being affected, and thus reducing the influence of the outside world on the immune cells during the freezing process.

[0016] 2. This scheme sets a volatilization tube, which runs through the connecting plate and the sealing plate. Since the freezing box cavity is filled with nitrogen liquid, which has a certain volatility, the nitrogen liquid evaporates to form nitrogen gas, which is discharged to the outside through the volatilization tube, and then the nitrogen liquid is infused into the freezing box cavity through the nitrogen liquid tank to ensure the stability of the air pressure in the freezing box cavity.

[0017] 3. This solution reduces the use of electronic components and mechanical parts, preventing a certain amount of water vapor from appearing during the freezing of immune cells. Water vapor can damage and corrode electronic components and mechanical parts, causing them to be unable to work normally, thereby increasing the service life of the device and reducing the use and maintenance costs of the device.

[0018] 4. This scheme arranges a tube body with porcelain beads in the cavity of the tube body, places the immune cell solution in the cavity of the tube body, and allows the immune cells to be adsorbed on the porcelain beads through the adsorption holes opened on the porcelain beads, thereby improving the adsorption rate and survival rate of the immune cells.

[0019] The specific implementation of the utility model is further described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In the attached picture:

[0021] Figure 1 It is a schematic diagram of distributed cooling components;

[0022] Figure 2 It is a partial cross-sectional view of a distributed cooling component;

[0023] Figure 3 It is a partial schematic diagram of the clamping device;

[0024] Figure 4It is a partial cross-sectional view of the engaging device;

[0025] Figure 5 Schematic diagram of cryopreservation tubes.

[0026] In the figure: 10, freezing box; 11, nitrogen liquid pipe; 12, valve; 13, sealing groove; 14, connecting groove; 15, connecting plate; 16, sealing plate; 17, volatilization tube; 18, movable groove; 19, moving block; 20, movable rod; 21, limiting rod; 22, spring; 23, fixed plate; 24, limiting hole; 25, tube body; 26, tube cap; 27, scale; 28, porcelain bead. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. The following embodiments are used to illustrate the utility model.

[0028] A distributed cooling component, such as Figure 1 , Figure 2 As shown, it includes a fixed part for controlling the position of the immune cell container and a sealing plate adjustment plate for achieving the sealing purpose, the fixed part includes a freezing box 10, the cavity of the freezing box 10 is hollow, the sealing plate adjustment plate includes a connecting plate 15, the cavity of the connecting plate 15 is hollow, a sealing plate 16 is fixedly installed on the bottom wall of the connecting plate 15, and a clamping device for sealing the freezing box 10 is arranged in the cavity of the connecting plate 15, the clamping device includes a limiting rod 21 for connecting the connecting plate 15 with the freezing box 10 and a moving block 19 for controlling the movement of the limiting rod 21.

[0029] During the specific working process, the main material of the freezing box 10 is stainless steel. Stainless steel has strong toughness and can effectively protect the immune cells in the cavity. The cavity of the connecting plate 15 is hollow, so the corresponding connecting plate 15 is light in weight, which allows the staff to easily pick up the connecting plate 15. The locking device arranged in the cavity of the connecting plate 15 enables the connecting plate 15 to be locked with the freezing box 10, and the purpose of sealing the freezing box 10 is achieved through the bottom arrangement of the connecting plate 15.

[0030] like Figure 2 As shown, a sealing groove 13 is provided on the top wall of the freezing box 10, and the sealing groove 13 is T-shaped. Connecting grooves 14 are provided on the inner walls on both sides of the sealing groove 13. There are two connecting grooves 14 in total, and the two connecting grooves 14 are in parallel. The position of the sealing groove 13 corresponds to the position of the connecting plate 15.

[0031] During the specific working process, the sealing groove 13 opened on the top of the freezing box 10 corresponds to the position of the connecting plate 15, and the connecting groove 14 opened on the inner wall of the sealing groove 13 is used to connect with the locking device, thereby achieving the purpose of connecting the sealing groove 13 and the connecting plate 15.

[0032] like Figure 2-Figure 4 As shown, there are two limit rods 21, and the two limit rods 21 are movably installed in the cavity of the connecting plate 15. The position of the limit rod 21 corresponds to the position of the connecting groove 14. The limit rod 21 is movably installed in the corresponding connecting groove 14 cavity. The limit rods 21 all penetrate the connecting plate 15, and a movable rod 20 is fixedly installed on the top wall of one end of the limit rod 21 extending into the cavity of the connecting plate 15. A movable groove 18 is provided on the top wall of the connecting plate 15, and the position of the movable groove 18 corresponds to the position of the movable rod 20. The movable rod 20 is movably installed in the movable groove 18 cavity. The movable rod 20 all penetrates the corresponding movable groove 18, and a moving block 19 is fixedly installed on the top wall of the movable rod 20. The moving blocks 19 are movably installed on the top wall of the connecting plate 15. A spring 22 is fixedly installed on the outer wall of one end of the limit rod 21 extending into the cavity of the connecting plate 15, and the other end of the spring 22 is fixedly connected to the inner wall of the connecting plate 15.

[0033] During the specific working process, the movable block 19 is moved to drive the movable rod 20 to move, and the movable rod 20 moves to drive the limit rod 21 to move. The position of the limit rod 21 corresponds to the position of the connecting groove 14. After the limit rod 21 is completely received into the cavity of the connecting plate 15, the connecting plate 15 is placed in the cavity of the sealing groove 13. The elastic force of the spring 22 drives the limit rod 21 to move into the corresponding cavity of the connecting groove 14. When the limit rod 21 moves into the cavity of the connecting groove 14, the purpose of connecting the connecting plate 15 and the freezing box 10 is achieved.

[0034] like Figure 4 As shown, a volatilization tube 17 is fixedly installed on the top wall of the connecting plate 15 for volatilizing and discharging the nitrogen liquid in the cavity of the freezing box 10. The volatilization tube 17 is L-shaped, and the volatilization tube 17 passes through the corresponding connecting plate 15 and the sealing plate 16 and extends into the corresponding cavity of the freezing box 10.

[0035] During the specific working process, the volatilization tube 17 passes through the connecting plate 15 and the sealing plate 16. When the connecting plate 15 is connected to the cavity of the sealing groove 13, the volatilization tube 17 extends into the cavity of the freezing box 10. The nitrogen liquid in the cavity of the freezing box 10 evaporates outward through the volatilization tube 17, thereby stabilizing the air pressure in the cavity of the freezing box 10 to prevent the air pressure from being too high or too low, causing the freezing box 10 to deform and affecting the freezing of the immune cells in the cavity of the freezing box 10.

[0036] like Figure 1 and Figure 4As shown, a nitrogen liquid pipe 11 is fixedly installed on one side wall of the freezing box 10, and the nitrogen liquid pipe 11 runs through the freezing box 10. A valve 12 is fixedly installed on one end of the nitrogen liquid pipe 11 away from the freezing box 10, and a nitrogen liquid tank for replenishing nitrogen liquid in the cavity of the freezing box 10 is provided at the other end of the valve 12. A fixing plate 23 is fixedly installed in the cavity of the freezing box 10, and a limiting hole 24 is provided on the top wall of the fixing plate 23. There are a plurality of limiting holes 24, and the limiting holes 24 are distributed on the surface of the fixing plate 23 in a rectangular array.

[0037] In the specific working process, the nitrogen liquid pipe 11 runs through the freezing box 10. When the nitrogen liquid needs to be supplemented in the cavity of the freezing box 10, the nitrogen liquid tank is connected to the valve 12, and the valve 12 is opened to transport the nitrogen liquid to the cavity of the freezing box 10 through the nitrogen liquid pipe 11, thereby ensuring that the nitrogen liquid in the cavity of the freezing box 10 is sufficient. By setting the limiting holes 24, there are several limiting holes 24, and the limiting holes 24 limit the position of the corresponding tube body 25, so as to control the tube body 25 to be vertical in the cavity of the freezing box 10.

[0038] like Figure 5 As shown, a tube body 25 is movably installed in the cavity of the limiting hole 24, and there are several tube bodies 25. A tube cap 26 is movably installed on the top wall of the tube body 25. The tube body 25 and the tube cap 26 are threadedly connected. Scales 27 are arranged on the outer wall of the tube body 25. The scales 27 are evenly distributed on the outer wall of the corresponding tube body 25. Ceramic beads 28 are arranged in the cavity of the tube body 25. There are several ceramic beads 28, and adsorption holes are opened on the outer wall of the ceramic beads 28.

[0039] In the specific working process, the tube body 25 is sealed by the tube cap 26, and the position of the solution set in the cavity of the tube body 25 is observed through the scale 27, and the ceramic beads 28 are provided with adsorption holes to facilitate the adsorption of immune cells.

[0040] It is understood that the present invention is described by some embodiments, and those skilled in the art are aware that various changes or equivalent substitutions may be made to these features and embodiments without departing from the spirit and scope of the present invention. In addition, under the teachings of the present invention, these features and embodiments may be modified to adapt to specific circumstances and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the scope of protection of the present invention.

Claims

1. A distributed cooling component, comprising a fixed portion for position control of an immune cell container and a sealing plate adjustment plate for sealing, characterized in that: The fixed part comprises a freezing box (10), the cavity of the freezing box (10) is in a hollow state, the sealing plate adjustment plate comprises a connecting plate (15), the cavity of the connecting plate (15) is in a hollow state, a sealing plate (16) is fixedly installed on the bottom wall of the connecting plate (15), and a clamping device for sealing the freezing box (10) is arranged in the cavity of the connecting plate (15), and the clamping device comprises a clamping device for connecting the connecting plate (15) to the freezing box (10). A limiting rod (21) and a moving block (19) for controlling the movement of the limiting rod (21); a sealing groove (13) is provided on the top wall of the freezing box (10); the sealing groove (13) is T-shaped; connecting grooves (14) are provided on the inner walls on both sides of the sealing groove (13); there are two limiting rods (21), both limiting rods (21) are movably installed in the cavity of the connecting plate (15), and the position of the limiting rod (21) corresponds to the position of the connecting groove (14).

2. The distributed cooling component according to claim 1, characterized in that: There are two connecting grooves (14) in total. The two connecting grooves (14) are in a parallel state. The position of the sealing groove (13) corresponds to the position of the connecting plate (15). The limiting rod (21) is movably installed in the cavity of the corresponding connecting groove (14). The limiting rod (21) penetrates the connecting plate (15). A movable rod (20) is fixedly installed on the top wall surface of one end of the limiting rod (21) extending into the cavity of the connecting plate (15).

3. The distributed cooling component according to claim 2, characterized in that: A movable groove (18) is provided on the top wall surface of the connecting plate (15), the position of the movable groove (18) corresponds to the position of the movable rod (20), the movable rod (20) is movably installed in the cavity of the movable groove (18), the movable rod (20) passes through the corresponding movable groove (18), a movable block (19) is fixedly installed on the top wall surface of the movable rod (20), the movable block (19) is movably installed on the top wall surface of the connecting plate (15), a spring (22) is fixedly installed on the outer wall of one end of the limiting rod (21) extending into the cavity of the connecting plate (15), and the other end of the spring (22) is fixedly connected to the inner wall of the connecting plate (15).

4. The distributed cooling component according to claim 3, characterized in that: A volatilization tube (17) is fixedly mounted on the top wall of the connecting plate (15) for volatilizing and discharging the nitrogen liquid in the cavity of the freezing box (10); the volatilization tube (17) is L-shaped, and the volatilization tube (17) penetrates the corresponding connecting plate (15) and the sealing plate (16) and extends into the cavity of the corresponding freezing box (10).

5. The distributed cooling component according to claim 4, characterized in that: A nitrogen liquid pipe (11) is fixedly installed on one side wall of the freezing box (10), the nitrogen liquid pipe (11) runs through the freezing box (10), a valve (12) is fixedly installed on one end of the nitrogen liquid pipe (11) away from the freezing box (10), and a nitrogen liquid tank for replenishing nitrogen liquid in the cavity of the freezing box (10) is arranged at the other end of the valve (12), a fixing plate (23) is fixedly installed in the cavity of the freezing box (10), and a limiting hole (24) is provided on the top wall of the fixing plate (23), and there are a plurality of limiting holes (24), and the limiting holes (24) are distributed in a rectangular array on the surface of the fixing plate (23).

6. A cryopreservation tube, characterized in that: It includes the distributed cooling component described in claim 5, wherein a tube body (25) is movably installed in the cavity of the limiting hole (24), there are a plurality of tube bodies (25), and a tube cap (26) is movably installed on the top wall of the tube body (25), and the tube body (25) and the tube cap (26) are threadedly connected; scales (27) are arranged on the outer wall of the tube body (25), and the scales (27) are evenly distributed on the outer wall of the corresponding tube body (25), and porcelain beads (28) are arranged in the cavity of the tube body (25); there are a plurality of porcelain beads (28), and adsorption holes are opened on the outer wall of the porcelain beads (28).

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