Piece preparation device for culturing and observing single thalli or single spores

Abstract

The utility model belongs to the fungus slide preparation technology, and discloses a slide preparation device for single thallus or single spore culture observation, the slide preparation device comprises a culture dish and a glass slide, a removable bracket is arranged in the culture dish, the bracket comprises a bottom plate and a supporting plate, the bottom plate is in contact with the bottom of the culture dish to support the bracket, and the supporting plate is in contact with the bottom of the culture dish to support the bracket. The supporting plate is fixed on the bottom plate and is used for lifting the glass slide, so that the glass slide is lifted by a specified distance relative to the bottom of the culture dish; according to the utility model, the removable bracket is arranged in the culture dish, so that the glass slide is lifted, and the lifting gap of 5-8mm is reserved on the glass slide relative to the bottom of the culture dish, so that the end part of the clamp can enter any position of the lifting gap to quickly clamp the glass slide during clamping, the clamping efficiency and stability of the glass slide are improved, and the working efficiency is improved. The glass slide does not need to be moved in the clamping process, clamping points do not need to be found from multiple angles to ensure the clamping stability, and the defects in the prior art are overcome.

Description

Technical Field

[0001] The utility model belongs to the technology of making slices of fungi, in particular to a slice making device used for the observation of single bacterial body or single spore culture. Background Art

[0002] In order to save the repeated separation, purification and cultivation process and save time in the process of cultivation, separation and purification of microorganisms, it is usually necessary to separate a single bacterium or a single spore, make a slide, and then culture and observe it, so as to achieve rapid separation of a single bacterium or a single spore.

[0003] The preparation of microorganisms usually includes the use of culture dishes and slides. At present, slides are generally thin glass sheets with a thickness of about 5 mm, and are placed directly in culture dishes. When tweezers are used to pick up the slide for observation, one clamping end of the clamp needs to be inserted into the bottom of the slide and ensure that the clamping end is located in the non-sample area of ​​the slide before the slide can be taken out. One of the problems with this operation process is that the slide needs to be tilted at a certain angle, which may affect the original morphology of the microorganism and affect the observation and analysis results; the second problem is that it is necessary to pay attention to the selection of the clamping point. First, it does not affect the sample, and second, the clamping is stable, which makes the clamping of the slide take a long time. That is, when clamping the slide in the culture dish, there is a problem that the slide needs to be moved and the efficiency of the slide clamping is low.

[0004] Based on the above analysis, the present utility model application is to design a slide making device for observing single bacterial cell or single spore culture. Utility Model Content

[0005] The utility model aims to provide a slide making device for single bacterial cell or single spore culture observation, so as to solve the problems that the slide needs to be moved when clamping the slide in the culture dish and the slide clamping efficiency is low.

[0006] In order to solve the above problems, the utility model provides a slide-making device for single bacterial or single spore culture observation, including a culture dish and a glass slide, the culture dish is provided with a removable bracket, the bracket includes a base plate and a support plate, the base plate contacts the bottom of the culture dish to support the bracket, the support plate is fixed to the base plate to lift the glass slide, so as to raise the glass slide a specified distance relative to the bottom of the culture dish.

[0007] As a preferred solution of the utility model, a limiting U-shaped groove is provided at the position where the top of the support plate contacts the glass slide, the width of the limiting U-shaped groove is slightly larger than the width of the glass slide, and the limiting U-shaped groove is used to fix and limit the glass slide.

[0008] As a preferred solution of the utility model, an extension plate is provided on the top of the support plate, and the extension plate is hinged to the outer side of the top of the support plate through a hinge, and can be rotated and folded with the hinge as an axis.

[0009] As a preferred solution of the utility model, the support plate and the base plate are integrally formed to form a U-shaped or I-shaped structure.

[0010] As a preferred solution of the utility model, a plurality of continuously arranged square grooves with a depth of 3 mm and a length and width of 5 mm are provided in the center of the slide.

[0011] As a preferred solution of the utility model, position marks are provided on the square slots.

[0012] As a preferred solution of the utility model, the specified distance between the slide and the bottom of the culture dish is 5-8 mm.

[0013] Compared with the prior art, the advantages of this film making device are:

[0014] The utility model realizes the lifting of the slide glass by arranging a bracket in the culture dish, so that the slide glass is lifted with a lifting gap of 5-8mm relative to the bottom of the culture dish, so that the end of the clamp can enter any position of the lifting gap to quickly clamp the slide glass during clamping, thereby improving the efficiency and stability of slide glass clamping. There is no need to move the slide glass during clamping, and there is no need to find clamping points at multiple angles to ensure the stability of clamping, which solves the shortcomings of the prior art. In addition, the bracket of the utility model is removably arranged in the culture dish, and it can be selected whether to add the bracket according to actual test needs, thereby improving the flexibility of use. It can be seen that the use of the slide making device can realize the precise, accurate and stable clamping of the slide glass, effectively improving the clamping efficiency / slide making efficiency of the slide glass, thereby improving the test efficiency, and has certain market value and practical value. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the installation of the bracket of the utility model in a culture dish.

[0016] Figure 2 It is a schematic diagram of the overall structure of the bracket of the utility model.

[0017] Figure 3 For the utility model Figure 2 A local enlarged schematic diagram of point A in the middle.

[0018] Figure 4 For the utility model Figure 2 A local enlarged schematic diagram of point B in the middle.

[0019] Figure 5 This is a schematic diagram of the overall structure of another bracket of the utility model.

[0020] Figure 6 It is a schematic diagram of the main structure of the slide of the utility model.

[0021] Attached photos

[0022] 1 is a culture dish; 2 is a bracket; 21 is a bottom plate; 22 is a support plate; 23 is an extension plate; 3 is a hinge; 4 is a limiting U-shaped groove; 5 is a slide glass; and 6 is a square grid. DETAILED DESCRIPTION

[0023] The present invention will be further described in detail below in conjunction with specific implementations and with reference to the accompanying drawings. It should be emphasized that the following description is merely exemplary and is not intended to limit the scope of the present invention and its application.

[0024] This embodiment provides a slide making device for single bacterial cell or single spore culture observation, the device comprises a culture dish 1 and a slide glass 5, a removable bracket 2 is provided in the culture dish 1, such as Figure 1 As shown, it can be understood that the height of the bracket 2 should be less than the depth of the culture dish 1 to ensure that the normal packaging of the culture dish 1 is not affected. The bracket 2 can be made of glass, plastic or stainless steel, which can be selected according to actual needs.

[0025] The bracket 2 includes a bottom plate 21 and a support plate 22. Figure 2 Or as shown in 5, the bottom plate 21 contacts the bottom of the culture dish 1 to support the bracket 2. In order to improve the stability of the support, the length of the bottom plate 21 is preferably 3 / 5 of the inner diameter of the culture dish 1, and the width is 1 / 4 of the inner diameter of the culture dish 1; the support plate 22 is fixed above the bottom plate 21 and has a specified spacing relative to the bottom of the culture dish 1. The support plate 22 is used to lift the slide glass 5, that is, the support plate 22 is fixed above the bottom plate 21 to lift the slide glass 5, so as to raise the slide glass 5 relative to the bottom of the culture dish 1 by a specified distance to form a lifting gap. It can be understood that the size of the lifting gap should be at least larger than the thickness of the end of the clamp used to clamp the slide glass 5. In this embodiment, the size of the lifting gap is preferably 5-8mm.

[0026] It can be seen that the present embodiment realizes the lifting of the glass slide 5 by arranging the bracket 2 in the culture dish 1, so that the glass slide 5 has a lifting gap of 5-8mm relative to the bottom of the culture dish 1, so that when clamping, the end of the clamp enters any position of the lifting gap to quickly clamp the glass slide 5, thereby improving the efficiency and stability of clamping the glass slide 5. There is no need to move the glass slide 5 during clamping, and there is no need to find the clamping point at multiple angles to ensure the stability of clamping, which solves the shortcomings of the prior art. In addition, the bracket 2 of the present embodiment is removably arranged in the culture dish 1, and whether to add the bracket 2 can be selected according to the actual test needs, thereby improving the flexibility of use. It can be seen that the film making device of the present embodiment can accurately, precisely and stably realize the clamping of the glass slide 5, effectively improving the clamping efficiency of the glass slide 5, thereby improving the test efficiency, and has certain market value and practical value.

[0027] In this embodiment, the support plate 22 and the base plate 21 are fixed by plugging, which makes it easy to store the bracket 2; or, for the sake of ease of use, the support plate 22 and the base plate 21 are designed to be integrally formed. Since the overall size of the bracket 2 is small and easy to store, the base plate 21 and the support plate 22 are preferably integrally formed in this embodiment.

[0028] The bracket 2 formed by the support plate 22 and the base plate 21 is I-shaped or U-shaped. The present embodiment preferably has a U-shaped structure. The bracket 2 with this structure can reduce the contact area between the glass slide 5 and the support plate 22, especially the presence of the middle recessed part is more conducive to clamping the glass slide 5. On the other hand, compared with the I-shaped structure, it is lighter and can bear weight evenly.

[0029] A limiting U-shaped groove 4 is provided at the position where the top of the support plate 22 contacts the slide glass 5. Figure 5 As shown, the width of the limiting U-shaped groove 4 is slightly larger than the width of the glass slide 5. The glass slide 5 can be fixed and limited in the limiting U-shaped groove 4 through the limiting U-shaped groove 4 to prevent the glass slide 5 from shifting or slipping. It is understandable that the depth of the U-shaped groove should be slightly larger than the thickness of the glass slide 5, and in this embodiment it is preferably 8-10 mm. In addition, in this embodiment, it is preferred that the bracket 2 is suitable for conventional standard-sized glass slides 5, that is, suitable for glass slides 5 with a size of 25.4 mm × 76.2 mm. It is understandable that in order to meet the needs of glass slides 5 of different specifications, brackets 2 of various specifications that meet the needs of glass slides 5 of different sizes can be provided.

[0030] As a preferred embodiment of the utility model, in order to be able to adapt to culture dishes 1 or slides 5 of different specifications, an extension plate 23 is provided on the top of the support plate 22. The extension plate 23 is hinged to the outer side of the top of the support plate 22 through a hinge 3, and can be rotated, folded or stretched around the hinge 3 as an axis. Figure 3 and 42, which are schematic diagrams of the support plate 22 provided in this embodiment being folded and unfolded relative to the hinge 3. The extension plate 23 can adjust the size of the support 2 as required, thereby increasing the stability of the support to meet the use of culture dishes 1 and slides 5 of different specifications. In this embodiment, the hinge 3 is preferably a micro spring hinge or a folding hinge of an existing conventional structure.

[0031] As a preferred embodiment of the utility model, in order to further improve the gripping efficiency of the slide 5, a plurality of continuously arranged square grooves 6 with a depth of 3 mm and a length and width of 5 mm are provided in the center of the slide 5, such as Figure 6 As shown, by setting up the multiple square slots 6, on the one hand, the capacity of the slide glass 5 can be increased, so that multiple bacterial samples can be processed simultaneously on a single slide glass 5, thereby improving work efficiency. On the other hand, the samples can be separated to separate different bacterial samples or multiple samples of the same bacteria to avoid mutual interference and cross contamination between them. On the other hand, a clear boundary between the sample area and the non-sample area can be formed, which is convenient for the operator to quickly determine the non-sample area for clamping.

[0032] In this embodiment, in order to facilitate the positioning of each square slot 6 to improve the efficiency of microscopic observation, it is preferred that a position mark is provided on the square slot 6. The position mark is a number axis mark or a color mark. In this embodiment, the number axis mark is preferred.

[0033] The use principle of this embodiment includes: placing the bracket 2 in the culture dish 1 in advance, and adding 20% ​​glycerol to the bottom of the culture dish 1 to maintain the humidity of the culture medium on the slide 5 to prevent the culture medium on the slide 5 from drying up; according to the culture needs, first place one or more microbial cells or saturated bodies in the square slots 6 of the slide 5, and record the corresponding axis marks, then place the slide 5 on the bracket 2, and finally cover the culture dish 1 with a lid, seal it with a sealing film, and place it in an incubator for a certain period of time;

[0034] When observation is required, place the clamping end of one side of the slide glass 5 clamp at any position in the raised gap between the slide glass 5 and the culture dish 1, and ensure that the clamping point is in the non-sample area of ​​the slide glass 5. Then clamp the slide glass 5 and take it out and place it under the microscope, and find the corresponding square slot 6 according to the recorded numerical axis mark for observation.

[0035] The above is only an embodiment of the utility model, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several improvements can be made without departing from the premise of the utility model, which should also be regarded as the protection scope of the utility model, and these will not affect the effect of the implementation of the utility model and the practicality of the patent. The protection scope required by the utility model shall be based on the content of the claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A slide making device for observing single bacterial cell or single spore culture, comprising a culture dish and a slide glass, characterized in that: The culture dish is provided with a removable bracket, which includes a base plate and a support plate. The base plate contacts the bottom of the culture dish to support the bracket. The support plate is fixed above the base plate and has a specified distance relative to the bottom of the culture dish. The support plate is used to lift the slide.

2. The slide making device for single bacterial cell or single spore culture observation according to claim 1, characterized in that: A limiting U-shaped groove is provided at the position where the top of the support plate contacts the glass slide. The width of the limiting U-shaped groove is slightly larger than the width of the glass slide. The limiting U-shaped groove is used to fix and limit the glass slide.

3. The slide making device for single bacterial cell or single spore culture observation according to claim 1, characterized in that: An extension plate is provided on the top of the support plate. The extension plate is hinged to the outer side of the top of the support plate through a hinge and can be rotated with the hinge as an axis to achieve folding and extension.

4. The slide making device for single bacterial cell or single spore culture observation according to claim 1, characterized in that: The support plate and the bottom plate are integrally formed to form a U-shaped or I-shaped structure.

5. The slide making device for single bacterial cell or single spore culture observation according to claim 1, characterized in that: A plurality of continuously arranged square grooves with a depth of 3 mm and a length and width of 5 mm are arranged in the center of the glass slide.

6. The slide making device for single bacterial cell or single spore culture observation according to claim 5, characterized in that: Position marks are arranged on the square slots.

7. The slide making device for single bacterial cell or single spore culture observation according to claim 1, characterized in that: The support plate can lift the glass slide to 5-8 mm relative to the bottom of the culture dish.

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