Detection box for rapidly detecting prostate massage liquid and semen elastase

Abstract

The utility model discloses a detection box for rapidly detecting prostate massage liquid and semen elastase, which comprises a box body and a test strip, and the box body comprises a lower box body and an upper box body connected with the lower box body, the test strip comprises a substrate fixed on the lower box body, and a sample pad, a combination pad and a nitrocellulose membrane which are arranged on the substrate and are sequentially connected end to end, a treatment liquid layer is sprayed on the sample pad, an elastinase antibody coating marked by colloidal gold particles is sprayed on the combination pad, and the nitrocellulose membrane is coated on the treatment liquid layer. The prostate massage liquid and semen elastinase detection card has the characteristics of simplicity, rapidness and accuracy, can be used for qualitative or semi-quantitative detection of prostate massage liquid and semen elastinase in a family environment or a clinical field, is convenient to detect and simple to operate, can rapidly screen and monitor the infection condition of a male genital tract, and can be used for detecting the infection condition of the prostate massage liquid and the semen elastinase. And the privacy protection is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro test paper detection, in particular to a detection box for rapidly detecting prostate massage fluid and semen elastase. Background Art

[0002] Chronic genital tract inflammation is one of the common diseases of the male urogenital system, including chronic prostatitis, chronic epididymitis, chronic seminal vesiculitis, etc. Chronic genital tract inflammation causes more than 15% of male infertility. The disease has a long course and is prone to recurrence. However, due to the lack of obvious clinical manifestations of chronic genital tract inflammation and the lack of relatively specific prostatic fluid / semen and serum markers, chronic genital tract inflammation is often ignored. At present, the commonly used indicator for clinical diagnosis of male chronic genital tract inflammation is leukocyte peroxidase staining with o-toluidine. However, since it can only display neutrophils, but activated neutrophils that release their granules and other leukocyte types that do not contain peroxidase, such as lymphocytes, macrophages and dendritic cells, are not colored, its application value is still controversial.

[0003] Elastase is a specific protein, an enzyme that can hydrolyze elastin in the body, and is distributed in various tissues and cells such as neutrophils and macrophages. When there is a male reproductive system infection, neutrophils secrete a large amount of elastase to participate in the anti-inflammatory response of phagocytosing pathogens, and work together with other oxidizing substances to exert a local anti-inflammatory effect. Since elastase is a soluble protease, it is evenly distributed in prostatic fluid and semen, and is more accurate than white blood cells in diagnosing male reproductive system infections. Prostatic fluid and semen elastase can be used as sensitive and quantitative male reproductive tract infections, especially one of the important diagnostic indicators for latent infections. WHO recommends elastase as an indicator for the diagnosis and prognosis monitoring of dormant reproductive system infections. Utility Model Content

[0004] The purpose of this section is to summarize some aspects of the embodiments of the utility model and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the abstract of the specification and the name of the utility model of this application to avoid blurring the purpose of this section, the abstract of the specification and the name of the utility model, and such simplifications or omissions cannot be used to limit the scope of the utility model.

[0005] Therefore, the purpose of the utility model is to provide a detection kit for rapid detection of prostate massage fluid and semen elastase, which is simple to operate and accurate in detection, and can quickly screen and monitor the infection status of male reproductive tract.

[0006] In order to solve the above technical problems, according to one aspect of the present invention, the present invention provides the following technical solutions:

[0007] A detection kit for rapid detection of prostate massage fluid and semen elastase, comprising:

[0008] A box body, comprising a lower box body and an upper box body connected to the lower box body;

[0009] The test strip comprises a substrate fixed on the lower box body, and a sample pad, a binding pad, and a nitrocellulose membrane arranged on the substrate and connected end to end in sequence;

[0010] The sample pad is sprayed with a treatment liquid layer, the binding pad is sprayed with an elastase antibody coating labeled with colloidal gold particles, and the nitrocellulose membrane has a detection line and a control line.

[0011] As a preferred solution of the detection kit for rapid detection of prostate massage fluid and semen elastase described in the utility model, the test strip also includes an absorption pad arranged on the substrate and the head end of which is connected to the tail end of the nitrocellulose membrane.

[0012] As a preferred solution of the detection kit for rapid detection of prostate massage fluid and semen elastase described in the utility model, the upper box body is provided with an observation window located just above the nitrocellulose membrane.

[0013] As a preferred solution of the detection kit for rapid detection of prostate massage fluid and semen elastase described in the utility model, the observation window is made of a transparent acrylic plate.

[0014] As a preferred solution of the detection kit for rapid detection of prostate massage fluid and semen elastase described in the utility model, it also includes a leakage plugging device;

[0015] The upper box body is located directly above the sample pad and is provided with a cylinder extending into the box body;

[0016] The leakage plugging device comprises a ring connected to the top of the cylinder, a curved portion connected to the inner wall of the ring, and a plurality of elastic components connected to the bottom of the curved portion and surrounding in a ring shape, with a gap being provided between two adjacent elastic components;

[0017] Wherein, when in a free state, a plurality of the elastic components are enclosed and closed to form a cone shape, and the gap seam is hidden; when the elastic components are separated, the gap seam is expanded.

[0018] As a preferred solution of the detection kit for rapid detection of prostate massage fluid and semen elastase described in the utility model, the elastic component is made of plastic.

[0019] Compared with the prior art, the utility model has the following beneficial effects:

[0020] The utility model has the characteristics of simplicity, rapidity and accuracy, and can be used for qualitative or semi-quantitative detection of seminal plasma elastase in a home environment or a clinical field. The detection is convenient and the operation is simple. It can quickly screen and monitor the infection status of the male reproductive tract and improves the protection of privacy.

[0021] The utility model adopts an innovative design of the leakage plugger, so that after the test box is used, the test liquid or bacteria will not leak out or be released from the test box, and the test box can be directly discarded, thereby avoiding the test liquid from polluting the environment or the bacteria from infecting other people, and having high safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solution of the implementation of the utility model, the utility model will be described in detail below in combination with the drawings and detailed implementation. Obviously, the drawings described below are only some implementations of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0023] Figure 1 This is a schematic diagram of the external structure of a first embodiment of a detection kit for rapid detection of prostate massage fluid and semen elastase of the utility model;

[0024] Figure 2 This is a schematic structural diagram of the first embodiment of a detection kit for rapid detection of prostate massage fluid and semen elastase of the utility model, with the upper box body removed;

[0025] Figure 3 This is a schematic structural diagram of a second embodiment of a detection kit for rapid detection of prostate massage fluid and semen elastase according to the utility model;

[0026] Figure 4 This is a structural cross-sectional view of a second embodiment of a detection kit for rapidly detecting prostate massage fluid and semen elastase according to the utility model. DETAILED DESCRIPTION

[0027] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below with reference to the accompanying drawings.

[0028] Secondly, the present invention is described in detail with reference to the schematic diagram. When describing the implementation of the present invention, for the sake of convenience, the cross-sectional diagram showing the device structure will not be partially enlarged according to the general proportion, and the schematic diagram is only an example, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.

[0029] In order to make the purpose, technical solution and advantages of the present invention more clear, the implementation mode of the present invention will be further described in detail below with reference to the accompanying drawings.

[0030] The utility model provides a detection kit for rapidly detecting prostate massage fluid and semen elastase, which has the advantages of simple operation and accurate detection and can rapidly screen and monitor the infection status of male reproductive tract.

[0031] Figure 1-Figure 2 The structure diagram of the first embodiment of the utility model is a rapid detection kit for prostate massage fluid and semen elastase. Figure 1-Figure 2 The present embodiment is a detection kit for rapidly detecting prostate massage fluid and semen elastase, and its main body includes a box body 100 and a test strip 200.

[0032] The box body 100 is used to protect the test strip 200. In the present embodiment, the box body 100 includes a lower box body 110 and an upper box body 120 connected to the lower box body 110. Preferably, in the present embodiment, the upper box body 120 is provided with an observation window 120a located directly above the nitrocellulose membrane 240 for observing the detection line 240b and the control line 240a, wherein the observation window 120a is made of a transparent acrylic plate.

[0033] The test strip 200 includes a substrate 210 fixed on the lower box body 110, and a sample pad 220, a conjugation pad 230, and a nitrocellulose membrane 240 arranged on the substrate 210 and connected end to end. The sample pad 220 is sprayed with a treatment liquid layer to reduce the viscosity of the semen sample. The conjugation pad 230 is sprayed with an elastase antibody coating labeled with colloidal gold particles. When the semen sample flows through the conjugation pad 230, the colloidal gold conjugate will form an immune complex with the semen elastase to be tested and flow to the nitrocellulose membrane together with the test liquid. Membrane 240, the nitrocellulose membrane 240 has a detection line 240b and a control line 240a, the detection line 240b is used to determine the test result, and the control line 240a is used to determine whether the test is valid. Preferably, in this embodiment, the test strip 200 also includes an absorption pad 250 arranged on the substrate 210 and the head end is connected to the tail end of the nitrocellulose membrane 240. The absorption pad 250 allows as much test liquid as possible to flow through the nitrocellulose membrane 240, while washing away excess colloidal gold-labeled antibody conjugates to eliminate background interference.

[0034] The present embodiment is a detection kit for rapid detection of prostate massage fluid and semen elastase. When performing the detection, firstly, semen prostate massage fluid / semen is dripped onto the sample pad 220. The sample enters the binding pad 230 through chromatography and combines with the colloidal gold receptor on the binding pad 230 to form an immune complex. The complex continues to flow through the nitrocellulose membrane 240 and combines with the antibody on the nitrocellulose membrane 240 to form a red strip. The excess gold-labeled antibody conjugate flows to the absorption pad 250. Example

[0035] In the above-mentioned embodiment 1, after the semen prostate massage fluid / semen is dripped into the sample pad 220, if too much semen prostate massage fluid / semen is dripped, the sample pad 220 is not completely absorbed, and residual liquid will be stored on the sample pad 220. When the sample pad 220 is discarded after use, the test liquid may leak out of the test box and pollute the environment, or the bacteria in the test liquid in the test box may cause infection to other people. Therefore, in order to further improve the sealing and safety of the test box, in this embodiment, as shown in FIG. Figure 3 and Figure 4 As shown, it also includes a leakage plug 300. The upper box body 120 is located directly above the sample pad 220 and is provided with a cylinder 120b extending into the box body 100. The leakage plug 300 includes a ring 310 connected to the top of the cylinder 120b, a curved portion 320 connected to the inner wall of the ring 310, and a plurality of elastic components 330 connected to the bottom of the curved portion 320 and surrounded in a ring shape. A gap 330a is provided between two adjacent elastic components 330, wherein, when in a free state, the plurality of elastic components 330 are enclosed and closed to form a cone shape, and the gap 330a is hidden. When the elastic components 330 are separated, the gap 330a is expanded. In specific use, the gap 330a is The dropper of the liquid to be tested is inserted into the leakage stopper 300. At this time, due to the pressure of the periphery of the dropper on the elastic component 330, the elastic component 330 is stretched open and separated, and the gap 330a is enlarged. Then the dropper is squeezed to drop the test liquid onto the sample pad 220. When the dropper is pulled out from the elastic component 330, the elastic component 330 will return to its original closed state due to its own rebound force. At this time, multiple elastic components 330 are closed together to form a cone shape, the gap 330a is hidden, and then the cylinder 120b is closed, so that after the test box is used, the test liquid or bacteria will not leak or release from the test box and can be directly discarded.

[0036] Although the present invention has been described above with reference to the embodiments, various modifications may be made thereto and parts thereof may be replaced with equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed in the present invention may be used in combination with each other in any manner, and the fact that these combinations are not exhaustively described in this specification is only for the sake of omitting space and saving resources. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A rapid detection kit for prostate massage fluid and semen elastase, characterized in that: include: A box body (100) comprises a lower box body (110) and an upper box body (120) connected to the lower box body (110); A test strip (200) comprising a substrate (210) fixed on the lower box body (110), and a sample pad (220), a binding pad (230), and a nitrocellulose membrane (240) arranged on the substrate (210) and connected end to end in sequence; The sample pad (220) is sprayed with a treatment liquid layer, the binding pad (230) is sprayed with an elastase antibody coating labeled with colloidal gold particles, and the nitrocellulose membrane (240) has a detection line (240b) and a control line (240a).

2. A test kit for rapid detection of prostate massage fluid and semen elastase according to claim 1, characterized in that: The test strip (200) further comprises an absorption pad (250) which is arranged on the substrate (210) and has a head end connected to a tail end of the nitrocellulose membrane (240).

3. A test kit for rapid detection of prostate massage fluid and semen elastase according to claim 1, characterized in that: The upper box body (120) is provided with an observation window (120a) located directly above the nitrocellulose membrane (240).

4. A test kit for rapid detection of prostate massage fluid and semen elastase according to claim 3, characterized in that: The observation window (120a) is made of a transparent acrylic plate.

5. A test kit for rapid detection of prostate massage fluid and semen elastase according to claim 1, characterized in that: Also included is a leakage plugger (300); The upper box body (120) is located directly above the sample pad (220) and is provided with a cylinder (120b) extending into the box body (100); The liquid leakage plugging device (300) comprises a circular ring (310) connected to the top of the cylinder (120b), a curved portion (320) connected to the inner wall of the circular ring (310), and a plurality of elastic components (330) connected to the bottom of the curved portion (320) and surrounding in a ring shape, wherein a gap (330a) is provided between two adjacent elastic components (330); When in a free state, the plurality of elastic components (330) are closed together to form a cone shape, and the gap (330a) is hidden; when the elastic components (330) are separated, the gap (330a) is expanded.

6. A test kit for rapid detection of prostate massage fluid and semen elastase according to claim 5, characterized in that: The elastic component (330) is made of plastic.

Images