Electricity conversion cup

Abstract

The utility model relates to the technical field of biomedical equipment, in particular to an electric conversion cup. Comprising an electric conversion cup body and a stabilizing assembly, and the stabilizing assembly is connected with the electric conversion cup body and located below the electric conversion cup body; the stabilizing assembly comprises a trapezoidal seat, two limiting sleeves, two limiting blocks, a first connecting rod, two second connecting rods and a driving part, the trapezoidal seat is provided with a cavity and a through groove, the driving part is fixedly connected with the trapezoidal seat and located in the cavity, the middle of the first connecting rod is fixedly connected with the driving part, the two limiting blocks are movably connected with the trapezoidal seat, and the two limiting sleeves are movably connected with the trapezoidal seat. The second connecting rods are symmetrically arranged in the through groove, the two ends of each second connecting rod are rotationally connected with the first connecting rod and the corresponding limiting block respectively, and each limiting sleeve is fixedly connected with the corresponding limiting block and located above the trapezoidal base. The condition that the electric conversion cup body shakes is avoided, and normal operation of work is guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical equipment, in particular to an electroporation cup. Background Art

[0002] Electrotransformation is a transfection method that can easily and efficiently introduce foreign substances into cells. It is a highly versatile and simple solution that can be used to introduce nucleic acids, pharmaceutical ingredients, etc. into cells. Current research believes that electrotransformation is a transient stimulation of cells through an external electric field, which gives the cell membrane enough free energy to rearrange the cell membrane phospholipid molecules, resulting in the formation of hydrophobic holes on the cell membrane. Subsequently, molecules such as exogenous DNA, RNA, and drugs mixed in the electrotransformation buffer can enter the cell through the cell membrane holes (Neumann E, 1982). The instantaneous electric field applied during electrotransformation can cause a significant change in the pH value at the two electrodes, increasing cell death (Nordenstrom BE, 1994), and the electric heat generated at the same time will also increase cell death (Pliquett UF, 2002).

[0003] In the prior art patent CN210974699U discloses an electroporation cup, including a conductive sheet, an electrode, a bracket and an electroporation tank, wherein the conductive sheet is arranged on both sides of the bracket, the electroporation tank is arranged in the center of the bracket, and the electrode is arranged on the conductive sheet and symmetrically arranged at both ends of the electroporation tank. The electrode includes a cathode and an anode, one end of the cathode and the anode is symmetrically arranged on the top of the conductive sheet, and the other end is vertically arranged in the electroporation tank; an electroporation tank is arranged in the center of the electroporation tank, and the electroporation tank cooperates with one end of the electrode; and a concave structure for nesting with the electroporation tank is arranged on the bracket. By increasing the distance between the two electrodes and reducing the contact width between each electrode surface and the cell solution to be electroporated, cell death can be reduced and the electroporation efficiency of the cells can be increased.

[0004] However, in the above-mentioned prior art patents, the contact area of ​​the bottom of the electric conversion cup is small, and it is usually placed on a workbench. When the electric conversion cup is hit, it is easy to shake, which will affect the normal operation. Utility Model Content

[0005] The utility model aims to provide an electric conversion cup, which solves the problem that the contact area of ​​the bottom of the existing electric conversion cup is small and the electric conversion cup is usually placed on a workbench. When the electric conversion cup is hit, it is easy to shake, which will affect the normal operation.

[0006] To achieve the above-mentioned purpose, the utility model adopts an electroconversion cup, comprising an electroconversion cup body and a stabilizing component, wherein the stabilizing component is connected to the electroconversion cup body and is located below the electroconversion cup body;

[0007] The stabilizing assembly includes a trapezoidal seat, two limit sleeves, two limit blocks, a first connecting rod, two second connecting rods and a driving member. The trapezoidal seat has a cavity and a through groove. The driving member is fixedly connected to the trapezoidal seat and is located in the cavity. The middle part of the first connecting rod is fixedly connected to the driving member. The two limit blocks are movably connected to the trapezoidal seat and are symmetrically arranged in the through groove. The two ends of each of the second connecting rods are respectively rotatably connected to the first connecting rod and the corresponding limit block. Each of the limit sleeves is respectively fixedly connected to the corresponding limit block and is located above the trapezoidal seat.

[0008] Wherein, the stabilizing component also includes two extruded sponges, and the two extruded sponges are respectively fixedly connected to the corresponding limiting sleeves and are respectively located on the inner side walls of the corresponding limiting sleeves.

[0009] Wherein, the driving component includes a motor seat and a driving motor, the motor seat is fixedly connected to the trapezoidal seat and is located in the trapezoidal seat, the driving motor is fixedly connected to the motor seat and is located in the motor seat, and the middle part of the first connecting rod is fixedly connected to the output end of the driving motor.

[0010] Wherein, the electroporation cup further includes a plurality of groups of movable components, and the plurality of groups of movable components are all arranged below the trapezoidal seat.

[0011] Among them, the moving assembly includes a support, a threaded rod, a handwheel, a mounting seat and a moving wheel. The support is fixedly connected to the trapezoidal seat and is located on the outer side wall of the trapezoidal seat. The threaded rod is threadedly connected to the support and passes through the support. The handwheel is connected to one end of the threaded rod, and the mounting seat is connected to the other end of the threaded rod. The moving wheel is arranged below the mounting seat.

[0012] Wherein, the moving assembly further comprises an anti-slip sleeve, which is fixedly connected to the hand wheel and is located on the outer side wall of the hand wheel.

[0013] The utility model provides an electric conversion cup. When the electric conversion cup body needs to be used for operation, the electric conversion cup body is placed above the trapezoidal seat and located between the two limit sleeves. Then the driving member in the cavity is started. The driving member drives the first connecting rod to rotate. Then the two ends of the first connecting rod respectively pull the two second connecting rods. Each of the second connecting rods drives the limit block to slide in the through groove. Each of the limit blocks respectively drives the limit sleeve to move above the trapezoidal seat. At this time, the two limit sleeves move relatively above the trapezoidal seat until the two limit sleeves clamp the electric conversion cup body. The electric conversion cup body is fixed above the trapezoidal seat, and the trapezoidal seat supports the electric conversion cup body. Through the above method, the electric conversion cup body can be fixed firmly, the electric conversion cup body can be prevented from shaking, and the normal operation can be ensured. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 It is a schematic diagram of the overall structure of the first embodiment of the utility model.

[0016] Figure 2 It is a partial structural side view of the first embodiment of the utility model.

[0017] Figure 3 The utility model Figure 2 AA line structural section view.

[0018] Figure 4 It is a partial structural schematic diagram of the first embodiment of the utility model.

[0019] Figure 5 It is a schematic diagram of the overall structure of the second embodiment of the utility model.

[0020] 101-electric conversion cup body, 102-trapezoidal seat, 103-limiting sleeve, 104-limiting block, 105-first connecting rod, 106-second connecting rod, 107-squeezing sponge, 108-motor seat, 109-driving motor, 110-cavity, 111-through groove, 201-support, 202-threaded rod, 203-handwheel, 204-mounting seat, 205-moving wheel, 206-anti-slip sleeve. DETAILED DESCRIPTION

[0021] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0022] First embodiment

[0023] See also Figure 1 to Figure 4 ,in Figure 1 is a schematic diagram of the overall structure of the first embodiment, Figure 2 is a structural side view of part of the first embodiment, Figure 3 yes Figure 2 AA line structural section view, Figure 4 It is a partial structural diagram of the first embodiment.

[0024] The utility model provides an electric conversion cup: comprising an electric conversion cup body 101 and a stabilizing component, the stabilizing component comprising a trapezoidal seat 102, two limiting sleeves 103, two limiting blocks 104, a first connecting rod 105, two second connecting rods 106, a driving member and two extrusion sponges 107, the driving member comprising a motor seat 108 and a driving motor 109. The above-mentioned solution solves the problem that the contact area at the bottom of the existing electric conversion cup is small, and the electric conversion cup is usually placed on a workbench, and is prone to shaking when it is hit, which affects the normal operation.

[0025] For this specific embodiment, the stabilizing component is connected to the electrotransformation cup body 101 and is located below the electrotransformation cup body 101. The electrotransformation cup body 101 includes a conductive sheet, an electrode, a bracket and an electric shock tank. The conductive sheet is arranged on both sides of the bracket, the electric shock tank is arranged in the center of the bracket, and the electrode is arranged on the conductive sheet and symmetrically arranged at both ends of the electric shock tank. The electrode includes a cathode and an anode, one end of the cathode and the anode is symmetrically arranged on the top of the conductive sheet, and the other end is vertically arranged in the electric shock tank; an electric shock pool is arranged in the center of the electric shock tank, and the electric shock pool cooperates with one end of the electrode; a concave structure for nesting with the electric shock tank is arranged on the bracket. By increasing the distance between the two electrodes and reducing the contact width of each electrode surface with the cell solution to be electroshocked, the cell death can be reduced and the cell electrotransformation efficiency can be increased. The above method is already a prior art and will not be described in detail here.

[0026] Among them, the trapezoidal seat 102 has a cavity 110 and a through groove 111, the driving member is fixedly connected to the trapezoidal seat 102 and is located in the cavity 110, the middle part of the first connecting rod 105 is fixedly connected to the driving member, the two limit blocks 104 are movably connected to the trapezoidal seat 102, and are symmetrically arranged in the through groove 111, the two ends of each of the second connecting rods 106 are respectively rotatably connected to the first connecting rod 105 and the corresponding limit blocks 104, each of the limit sleeves 103 is respectively fixedly connected to the corresponding limit blocks 104, and are all located above the trapezoidal seat 102. When the electroconversion cup body 101 needs to be used for operation, the electroconversion cup body 101 is placed above the trapezoidal seat 102 and located between the two limit sleeves 103, and then the driving member in the cavity 110 is started. The driving member will drive the first connecting rod 105 to rotate, and then the two ends of the first connecting rod 105 will respectively pull the two second connecting rods 106, and each of the second connecting rods 106 will drive the limit block 104 to slide in the through groove 111, and each of the limit blocks 104 will respectively drive the limit sleeve 103 to move above the trapezoidal seat 102. At this time, the two limit sleeves 103 move relatively above the trapezoidal seat 102 until the two limit sleeves 103 clamp the electroconversion cup body 101, and the electroconversion cup body 101 is fixed above the trapezoidal seat 102, and the trapezoidal seat 102 supports the electroconversion cup body 101. Through the above method, it is achieved that the electroconversion cup body 101 can be fixed firmly, the shaking of the electroconversion cup body 101 is avoided, and the normal operation is ensured.

[0027] Secondly, the two extruded sponges 107 are fixedly connected to the corresponding limiting sleeves 103 and are respectively located on the inner walls of the corresponding limiting sleeves 103. By arranging the extruded sponges 107 on the inner walls of the limiting sleeves 103, the extruded sponges 107 can increase the friction between the limiting sleeves 103 and the electroconversion cup body 101, and can fix the electroconversion cup body 101 more stably.

[0028] At the same time, the motor seat 108 is fixedly connected to the trapezoidal seat 102 and is located in the trapezoidal seat 102, the drive motor 109 is fixedly connected to the motor seat 108 and is located in the motor seat 108, the middle part of the first connecting rod 105 is fixedly connected to the output end of the drive motor 109, the motor seat 108 fixedly supports the drive motor 109 in the cavity 110, and the drive motor 109 controls the rotation of the first connecting rod 105.

[0029] When the electroconversion cup body 101 is needed to be operated, the electroconversion cup body 101 is placed above the trapezoidal seat 102 and located between the two limit sleeves 103, and then the drive motor 109 in the cavity 110 is started, and the drive motor 109 drives the first connecting rod 105 to rotate, and then the two ends of the first connecting rod 105 respectively pull the two second connecting rods 106, and each of the second connecting rods 106 drives the limit block 104 to slide in the through groove 111, and each of the limit blocks 104 respectively drives the limit sleeve 103 to move above the trapezoidal seat 102, and at this time, the two limit sleeves 103 are in the trapezoidal seat. The electric conversion cup body 101 is relatively moved above the trapezoidal seat 102 until the two limiting sleeves 103 clamp the electric conversion cup body 101, and the electric conversion cup body 101 is fixed above the trapezoidal seat 102, and the trapezoidal seat 102 supports the electric conversion cup body 101; in addition, by arranging the squeezing sponge 107 on the inner wall of the limiting sleeve 103, the squeezing sponge 107 can increase the contact friction between the limiting sleeve 103 and the electric conversion cup body 101, and can fix the electric conversion cup body 101 more stably. Through the above method, it is achieved that the electric conversion cup body 101 can be firmly fixed, the shaking of the electric conversion cup body 101 is avoided, and the normal operation is guaranteed.

[0030] Second embodiment

[0031] See also Figure 5 ,in Figure 5 It is a schematic diagram of the overall structure of the second embodiment.

[0032] On the basis of the first embodiment, an electroconversion cup of the utility model further includes a plurality of moving components, wherein the moving components include a support 201 , a threaded rod 202 , a hand wheel 203 , a mounting seat 204 , a moving wheel 205 and an anti-slip sleeve 206 .

[0033] A plurality of groups of the moving components are arranged below the trapezoidal seat 102. By arranging the moving components below the trapezoidal seat 102, the trapezoidal seat 102 and the electroporation cup body 101 can be easily moved through the moving components, and the operation is simple.

[0034] The support 201 is fixedly connected to the trapezoidal seat 102 and is located on the outer wall of the trapezoidal seat 102. The threaded rod 202 is threadedly connected to the support 201 and passes through the support 201. The hand wheel 203 is connected to one end of the threaded rod 202. The mounting seat 204 is connected to the other end of the threaded rod 202. The moving wheel 205 is arranged below the mounting seat 204. When the trapezoidal seat 102 and the electroconversion cup body 101 are moved, , shake the multiple hand wheels 203 respectively, each of the hand wheels 203 drives the threaded rod 202 to move in the support 201 and move downward on the support 201, and the mounting seat 204 drives the moving wheel 205 to move until the multiple legs under the trapezoidal seat 102 leave the ground, and then move the trapezoidal seat 102 by pulling. In this way, the trapezoidal seat 102 and the electric conversion cup body 101 can be easily moved, and the operation is simple.

[0035] The anti-skid cover 206 is fixedly connected to the hand wheel 203 and is located on the outer side wall of the hand wheel 203. By arranging the anti-skid cover 206 on the hand wheel 203, the anti-skid cover 206 can increase the comfort and anti-skid effect of the hand wheel 203 in use.

[0036] When moving the trapezoidal seat 102 and the electroconversion cup body 101, the multiple hand wheels 203 are shaken respectively, and each of the hand wheels 203 drives the threaded rod 202 to move in the support 201 and move downward on the support 201. The mounting seat 204 drives the moving wheel 205 to move until the multiple legs under the trapezoidal seat 102 leave the ground, and then the trapezoidal seat 102 is moved by pulling; in addition, by arranging the anti-slip cover 206 on the hand wheel 203, the anti-slip cover 206 can increase the comfort and anti-slip effect of the hand wheel 203 during use. In this way, the trapezoidal seat 102 and the electroconversion cup body 101 can be easily moved, and the operation is simple.

[0037] The above disclosures are only two preferred embodiments of the present invention, which certainly cannot be used to limit the scope of the present invention. A person skilled in the art can understand that all or part of the processes of the above embodiments and equivalent changes made according to the claims of the present invention still fall within the scope of the present invention.

Claims

1. An electroconversion cup, characterized in that: It includes an electroconversion cup body and a stabilizing component, wherein the stabilizing component is connected to the electroconversion cup body and is located below the electroconversion cup body; The stabilizing assembly includes a trapezoidal seat, two limit sleeves, two limit blocks, a first connecting rod, two second connecting rods and a driving member. The trapezoidal seat has a cavity and a through groove. The driving member is fixedly connected to the trapezoidal seat and is located in the cavity. The middle part of the first connecting rod is fixedly connected to the driving member. The two limit blocks are movably connected to the trapezoidal seat and are symmetrically arranged in the through groove. The two ends of each of the second connecting rods are respectively rotatably connected to the first connecting rod and the corresponding limit block. Each of the limit sleeves is respectively fixedly connected to the corresponding limit block and is located above the trapezoidal seat.

2. The electroconversion cup according to claim 1, characterized in that: The stabilizing component also includes two extruded sponges, which are respectively fixedly connected to the corresponding limiting sleeves and are respectively located on the inner side walls of the corresponding limiting sleeves.

3. The electroconversion cup according to claim 2, characterized in that: The driving member includes a motor seat and a driving motor. The motor seat is fixedly connected to the trapezoidal seat and is located in the trapezoidal seat. The driving motor is fixedly connected to the motor seat and is located in the motor seat. The middle part of the first connecting rod is fixedly connected to the output end of the driving motor.

4. The electroporation cup according to claim 1, characterized in that: The electroconversion cup further comprises a plurality of groups of moving components, and the plurality of groups of moving components are all arranged below the trapezoidal seat.

5. The electroconversion cup according to claim 4, characterized in that: The moving assembly includes a support, a threaded rod, a handwheel, a mounting seat and a moving wheel. The support is fixedly connected to the trapezoidal seat and is located on the outer side wall of the trapezoidal seat. The threaded rod is threadedly connected to the support and passes through the support. The handwheel is connected to one end of the threaded rod, and the mounting seat is connected to the other end of the threaded rod. The moving wheel is arranged below the mounting seat.

6. The electroconversion cup according to claim 5, characterized in that: The moving assembly also includes an anti-slip sleeve, which is fixedly connected to the hand wheel and is located on the outer side wall of the hand wheel.

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