Fixing device for biological material analysis

Abstract

The utility model provides a fixing device for biological material analysis, comprising: a base for assembling on a cold table of an electron microscope chamber; the two pressing blocks are oppositely arranged to form a clamping space, and the clamping space is configured to clamp a biological sample ring; the clamping elastic sheet is connected with the pressing block and the base; and when the biological sample ring is fed into the clamping space, the clamping elastic piece automatically adjusts the clamping force of the clamping space by utilizing the elastic force of the clamping elastic piece so as to horizontally clamp the biological sample ring. According to the fixing device provided by the invention, the problems of falling, fuzzy imaging, sample damage and the like when a biological sample ring is fixed on a traditional sample table are solved.

Description

Technical Field

[0001] The present application relates to the field of biological sample analysis equipment, and in particular to a fixing device for biological material analysis. Background Art

[0002] As an important instrument for analyzing biological materials, cryo-electron microscopy is often used to observe and study the microstructure of biological samples. In cryo-electron microscopy, in order to maintain the natural state of biological samples and prevent their inactivation and denaturation while performing high-precision sample observation, it is usually necessary to place the biological samples on a biological sample ring, use the biological sample ring to transfer the biological samples, and fix the biological sample ring on a stable sample stage for observation. The commonly used method is to stick the biological sample ring to the sample stage with conductive glue, but this method has the problem of easy sample detachment and blurred imaging, and causes potential damage to the surface of the sample, which greatly affects the quality and effect of the observed image. Utility Model Content

[0003] In view of the above problems, the utility model provides a fixing device for biological material analysis to solve the problems of falling off, blurred imaging and sample damage in the traditional sample stage fixing biological sample ring.

[0004] The technical solution of the utility model is:

[0005] A fixing device for biological material analysis, comprising:

[0006] A base, used to be assembled to the cold stage of the electron microscope chamber;

[0007] Two pressing blocks arranged opposite to each other form a clamping space, wherein the clamping space is configured to clamp a biological sample circle;

[0008] A clamping spring piece connects the pressing block and the base;

[0009] When the biological sample circle is sent into the clamping space, the clamping spring automatically adjusts the clamping force of the clamping space by using its own elastic force to horizontally clamp the biological sample circle.

[0010] As one of the preferred solutions, two clamping springs are provided, and the two clamping springs are connected to the two pressing blocks in a one-to-one correspondence.

[0011] As one of the preferred solutions, the clamping spring is divided into a first part and a second part by a rectangular groove, the first part is fixed on the pressing block, and the second part is fixed on the base;

[0012] Wherein, the first part is located above the second part.

[0013] As one of the preferred solutions, the rectangular groove extends from one end of the clamping spring sheet to the other opposite end and has a distance from the other end.

[0014] As one of the preferred solutions, the height of the rectangular groove is smaller than the height of the first part and the second part, and the height of the first part and the height of the second part are the same or different.

[0015] As one of the preferred solutions, the pressing block is in sliding contact with the base.

[0016] As one of the preferred solutions, the base is made of a composite of a heat-conducting metal material and a heat-insulating plastic, and the clamping spring is made of a metal or an alloy.

[0017] As one of the preferred solutions, the clamping spring is detachably connected to the pressing block and the base respectively.

[0018] As one of the preferred solutions, the fixing device further comprises:

[0019] At least one fastening bolt, at least one first fastening hole is provided on the clamping spring piece, at least one second fastening hole is provided on the base and the pressing block respectively, each of the fastening bolts passes through the first fastening hole and the second fastening hole in sequence to connect the clamping spring piece with the base and the pressing block.

[0020] As one of the preferred solutions, a groove is provided on one side of the clamping space formed by the two pressing blocks, the length of the groove is the same as the length of the pressing block, and the inner edge shape of the two grooves matches the outer edge shape of the biological sample circle.

[0021] Compared with the prior art, this application has the following advantages:

[0022] The utility model provides a fixing device for biological material analysis, comprising:

[0023] A base, used to be assembled to the cold stage of the electron microscope chamber;

[0024] Two pressing blocks arranged opposite to each other form a clamping space, and the clamping space is configured to clamp the biological sample circle; a clamping spring connects the pressing block and the base; wherein, when the biological sample circle is sent into the clamping space, the clamping spring automatically adjusts the clamping force of the clamping space by its own elastic force to horizontally clamp the biological sample circle.

[0025] By adopting the technical solution of the present application, through the combination of the base, the clamping spring and the pressure block, there is no need to use an additional driving mechanism for clamping. The clamping spring has a positioning and adjustment function, which can automatically adjust the sample that is not fed horizontally to make it horizontal and stable, thereby achieving stable fixation of the biological sample circle, thereby improving the imaging effect. Its overall structure is simple and easy to process, low cost, and can be applied to various scanning electron microscopes. It can directly fix the biological sample circle without the use of conductive glue. The installation is convenient and simple, and the biological sample circle that is easy to fall off is firmly fixed. The imaging effect is good, and the sample does not need to be strictly prepared, which reduces the difficulty of preparation and the cost of sample preparation. In this way, by providing a more stable and reliable sample fixing device, the quality of the observed image is improved, while the difficulty of operation and the complexity of sample preparation are reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solution of the present application, the drawings required for use in the description of the present application will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0027] Figure 1 It is an assembly diagram of a fixing device and a biological sample circle for biological material analysis according to an embodiment of the present application;

[0028] Figure 2 is a schematic diagram of the overall structure of a base according to another embodiment of the present application;

[0029] Figure 3 is a schematic diagram of the overall structure of the clamping spring sheet according to another embodiment of the present application;

[0030] Figure 4 It is a schematic diagram of the overall structure of a pressing block described in another embodiment of the present application.

[0031] Description of reference numerals:

[0032] 1. Clamping spring; 11. First part; 12. Second part; 13. Rectangular groove; 2. Press block; 21. Groove; 3. Biological sample circle; 4. Base; 41. Fixing hole; 42. Fixing column; 5. Second fastening hole; 6. First fastening hole. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0034] Reference Figure 1 As shown, Figure 1 This is an assembly diagram of a fixing device and a biological sample ring for biological material analysis shown in the utility model. Figure 1 As shown, the utility model provides a fixing device for biological material analysis, comprising: a base 4 for being assembled to a cold stage of an electron microscope chamber; two pressing blocks 2 arranged opposite to each other, forming a clamping space, wherein the clamping space is configured to clamp a biological sample circle 3 and a clamping spring 1, connecting the pressing block 2 and the base 4; wherein, when the biological sample circle 3 is sent into the clamping space, the clamping spring 1 automatically adjusts the clamping force of the clamping space by using its own elastic force, so as to horizontally clamp the biological sample circle 3.

[0035] Specifically, in this embodiment, the frozen biological sample is usually placed in the biological sample circle 3. The bottom of the base 4 can be fixedly connected to the cold stage in the electron microscope chamber by bolts, which plays a role in supporting and stabilizing the entire fixing device. Specifically, one end of the base 4 has two fixing holes 41 fixed to the electron microscope sample stage, and the bottom of the base 4 is fixedly connected to the cold stage in the electron microscope chamber by bolts passing through the fixing holes 41, thereby indirectly carrying the biological sample circle 3 in the electron microscope chamber and cooperating with the electron microscope to perform biological material analysis.

[0036] Specifically, two pressing blocks 2 are designed on the base 4, which are arranged opposite to each other on the base 4 to form a clamping space, which is an area formed by the opposite sides of the two pressing blocks 2 and the outside world. Among them, the clamping spring 1 is connected to the base 4 and the pressing block 2 respectively, and the clamping spring 1 is designed with a fastening hole for connection, and the hole diameter and position match the fastening holes on the pressing block 2 and the base 4.

[0037] Among them, since the clamping spring piece 1 is connected to the pressing block 2, and the pressing block 2 has no mechanical connection with the base 4, when the biological sample circle 3 is sent in, the pressing block 2 is subjected to the extrusion force, the clamping spring piece 1 and the pressing block 2 are propped up, and the clamping space is expanded, so that the biological sample circle 3 can be smoothly inserted; after the biological sample circle 3 is smoothly inserted into the clamping space, the clamping spring piece 1 has a tendency to shrink the clamping space through its own elasticity, and the biological sample circle 3 is then subjected to the extrusion force of the pressing block 2, so that the clamping force in the clamping space can clamp the biological sample circle 3 sent in without being too tight, thereby avoiding excessive extrusion damage to the biological sample circle 3 and protecting the integrity of the sample.

[0038] For biological sample circles 3 that are not fed in horizontally, the elastic force of the clamping spring 1 can realize the self-positioning of the biological sample circle 3, so that the sample is aligned during the fixing process, ensuring that the sample can be fixed horizontally and stably even if it is not fed in horizontally, reducing the shaking of the sample during the observation process, and improving the stability and clarity of the imaging. In addition, the pressing block 2 can be expanded and retracted within the elastic range of the clamping spring 1, so that the clamping space can adapt to biological sample circles 3 of different shapes and sizes, and has a wider range of applicability.

[0039] In summary, compared to the use of conductive glue, the fixing device provided by the embodiment of the utility model is easy to install and can firmly fix the biological sample circle 3 that is easy to fall off and has a good imaging effect. It allows the sample to be prepared without strict preparation, reducing the difficulty of preparation and the cost of sample preparation. The base 4, the clamping spring 1 and the pressing block 2 are all simple in structure, easy to process and low in cost. They are suitable for various scanning electron microscopes, especially for loading and fixing the biological sample circle 3 in the cavity of a cryo-electron microscope, so as to observe biological samples and keep the sample motionless relative to the sample stage during the observation process. It can effectively solve the problem of sample shedding or sample shaking caused by the inability of the sample stage to stably place the sample, which is not conducive to high-precision and high-magnification shooting of the sample, improve the observation quality and observation effect of the scanning electron microscope, and provide a stable, simple and cost-effective solution for biological material analysis.

[0040] In some embodiments, a clamping spring 1 can connect a pressing block 2 and a base 4, and the clamping force can be adjusted by adjusting the distance that a pressing block 2 is stretched and retracted.

[0041] In a preferred embodiment, two clamping springs 1 are provided, and the two clamping springs 1 are connected to the two pressing blocks 2 in a one-to-one correspondence. Figure 2 As shown, Figure 2 It is a schematic diagram of the overall structure of the base 4, in which the cold stage fixing end of the base 4 is fixed to the cold stage via two fixing holes 41 arranged at intervals in the transverse direction, and the spring piece fixing end of the base 4 is two fixing columns 42 arranged at intervals in the transverse direction, and two pressing blocks 2 are correspondingly disposed above the two fixing columns 42, and two clamping spring pieces 1 are respectively fitted and fixed on the two pressing blocks 2, and the biological sample circle 3 is sent from the outside to the inside into the clamping space formed by the pressing blocks 2.

[0042] Among them, the clamping spring 1 has a symmetrical structure and is made of metal or alloy. Preferably, it is made of 0.15mm thick beryllium bronze, and has sufficient elasticity to allow the pressure block 2 fixed thereon to be stretched open and to clamp the biological sample circle 3 sent in without being too tight. The relatively symmetrical arrangement of the clamping spring 1 and the pressure block 2 allows the clamping spring 1 to apply the same elastic force on both sides when the biological sample circle 3 is sent into the clamping space, so that the biological sample circle 3 can be clamped evenly to avoid sample damage caused by local over-tightening, and at the same time help maintain the balance between the two pressure blocks 2 and reduce the instability caused by the asymmetric design.

[0043] It can be understood that beryllium bronze is a material with good elasticity and strength. A thickness of 0.15 mm can provide appropriate elasticity, which is enough to be stretched open with the pressing block 2 and clamp the biological sample ring 3, but not too tight to damage the sample.

[0044] It should be noted that the base 4 is connected to the cold stage in the electron microscope chamber, and the sample in the cryo-electron microscope needs to be observed at low temperature. In the present embodiment, the base 4 is made of a composite of a heat-conducting metal material and a heat-insulating plastic. The heat-conducting metal material includes materials with good thermal conductivity such as copper, aluminum, silver, gold, etc., so that when the low-temperature sample stage is working, the low temperature of the cold stage can be effectively transferred to the sample stage, thereby maintaining the low temperature state of the biological sample circle 3 during the observation process. At the same time, metals such as copper, aluminum, silver, and gold have high mechanical strength and stability, which can be easily fixedly connected to the cold stage in the electron microscope chamber by bolts to provide sufficient mechanical stability to bear the weight of the pressing block 2, the biological sample circle 3, and the clamping shrapnel 1.

[0045] In addition to thermal conductivity, the base 4 also has thermal insulation properties to reduce heat loss and ensure that the device can maintain the required low-temperature environment during long-term operation. Among them, engineering plastics usually have good thermal insulation properties, which can reduce heat exchange with the external environment and help ensure that the biological sample circle 3 maintains a stable low-temperature state in the cryo-electron microscope.

[0046] It should be explained that a base 4 integrating cooling and heat preservation can be obtained by adding a filler with good thermal conductivity, such as metal powder, to the engineering plastic matrix. In this process, the degree of cooling and heat preservation can be balanced by controlling the content and distribution of the filler, so as to achieve good cooling and heat preservation performance at the same time.

[0047] In some embodiments, the composite material can be designed as a multi-layer structure, wherein one layer has high thermal conductivity for rapid transfer of cold, which is in contact with the cold stage, and another layer has thermal insulation for preventing heat from being dissipated to the external environment.

[0048] In some embodiments, the base 4 made of metal material may be surface treated except for the side in contact with the cold stage, that is, sprayed with engineering plastic, so that it absorbs the coldness of the cold stage while preventing heat loss in other directions.

[0049] In some embodiments, only the base 4 made of thermally conductive metal material may be used.

[0050] Preferably, when making the base 4, the pressing block 2 and the clamping spring 1 of the utility model, easy-to-process and low-cost materials can be used. On this basis, materials with good electrical conductivity and thermal conductivity can be selected to effectively control the temperature of the sample during the observation process to avoid a large amount of heat leakage.

[0051] See also Figure 3 As shown, Figure 3 The overall structural diagram of the clamping spring piece 1 is shown. In a further technical solution, the clamping spring piece 1 is divided into a first part 11 and a second part 12 by a rectangular groove 13, the first part 11 is fixed on the pressing block 2, and the second part 12 is fixed on the base 4;

[0052] The first part 11 is located above the second part 12. In this embodiment, the clamping spring piece 1 is a rectangular sheet, which is divided into the first part 11 and the second part 12 by the rectangular groove 13. The second part 12 is connected to the spring piece fixed end of the base 4 as a fixed end, and the first part 11 is connected to a side wall of the pressing block 2 away from the clamping space, so that when the biological sample circle 3 is sent into the clamping space, the second part 12 is used as an elastic support part to connect the pressing block 2 and the base 4 as a whole, and the first part 11 is used as an elastic adjustment part. As the pressing block 2 is stretched and retracted, a part of the clamping spring piece 1 is allowed to move with the pressing block 2, while the other part remains fixed.

[0053] For example, the clamping spring 1 is divided into two parts, upper and lower, by a rectangular groove 13 with a length of 7 mm and a width of 0.2 mm. The pressing block 2 is connected to the upper part (i.e., the first part), so that the center of the biological sample circle 3 is suspended, thereby maximally protecting the biological sample circle 3 from damage or reducing the risk of contamination caused by physical contact.

[0054] In summary, by fixing the bottom of the base 4 and connecting it to the cold stage in the electron microscope chamber, the pressing block 2 can be suspended above the sample stage, so that the center of the sample sent in is suspended in the air, thereby protecting the biological sample circle 3 from damage to the greatest extent, and having important application value in the analysis and observation of the biological sample circle 3.

[0055] In some embodiments, the rectangular groove 13 is located in the middle of the clamping spring sheet 1, that is, the height and / or area of ​​the first part 11 and the second part 12 are the same; in some embodiments, the rectangular groove 13 is located at a position where the clamping spring sheet 1 is closer to the second part 12, that is, the area and / or height of the first part 11 is greater than the area and / or height of the second part 12; in some embodiments, the rectangular groove 13 is located at a position where the clamping spring sheet 1 is closer to the first part 11, that is, the area and / or height of the second part 12 is greater than the area and / or height of the first part 11. In some embodiments, the height of the rectangular groove 13 is less than the height of the first part 11 and the second part 12.

[0056] In some embodiments, the length of the rectangular groove 13 is less than the length of the clamping spring 1, and the thickness of the rectangular groove 13 is the same as the thickness of the clamping spring 1, that is, the rectangular groove 13 partially penetrates the clamping spring 1. In other words, the rectangular groove 13 extends from one end of the clamping spring 1 to the other end opposite thereto, and has a distance from the other end. In this embodiment, one side of the first part 11 and the second part 12 of the clamping spring 1 are connected as a whole, and the other side is separated by the rectangular groove 13, forming an integrated separation structure as a whole, while the clamping spring 1 fixes the pressing block 2 on the base 4, to ensure that the upper and lower parts have sufficient contact area and elastic deformation space when clamped.

[0057] As a specific explanation of this embodiment, the pressing block 2 is in sliding contact with the base 4. In combination with the above embodiments, two fixing columns 42 are provided at the spring-piece fixing end of the base 4. The two fixing columns 42 are rectangular columns, one end of which extends laterally outward, and the other end is fixed to the side wall of the cold stage fixing end of the base 4. Among them, the pressing block 2 is a rectangular block, and the bottom wall surface of each pressing block 2 is in contact with the top wall surface of each fixing column 42, and one side wall surface is in contact with the side wall of the cold stage fixing end, so that the pressing block 2 slides along the base 4 during the process of being stretched and retracted, and after clamping the biological sample circle 3, it is supported by the clamping spring 1 and the base 4 at the same time.

[0058] More specifically, the clamping spring piece 1 is detachably connected to the pressing block 2 and the base 4. The fixing device further includes: at least one fastening bolt, at least one first fastening hole 6 is provided on the clamping spring piece 1, at least one second fastening hole 5 is provided on the base 4 and the pressing block 2, each of the fastening bolts passes through the first fastening hole 6 and the second fastening hole 5 in sequence to connect the clamping spring piece 1 to the base 4 and the pressing block 2.

[0059] In this embodiment, the first fastening hole 6 passes through the clamping spring piece 1, and the second fastening hole 5 may or may not pass through the corresponding base 4 and the pressing block 2. In combination with the above embodiments, a plurality of first fastening holes 6 are respectively arranged on the first part 11 and the second part 12 of the clamping spring piece 1. The number and size of the first fastening holes 6 located on the first part 11 may be the same as or different from the number and size of the first fastening holes 6 located on the second part 12.

[0060] In combination with the above embodiments, two first fastening holes 6 arranged laterally spaced apart are provided on the first part 11 and the second part 12 of the clamping spring piece 1, two second fastening holes 5 are provided on the pressing block 2, the two first fastening holes 6 of the first part 11 are opposite to the two second fastening holes 5 on the pressing block 2, two second fastening holes 5 are provided on the two fixing columns 42 of the base 4, and the two first fastening holes 6 of the second part 12 are opposite to the two second fastening holes 5 on the base 4.

[0061] The base 4 and one pressing block 2 are respectively connected to one clamping spring sheet 1 by four fastening bolts passing through the four first fastening holes 6 on each clamping spring sheet 1, the two second fastening holes 5 on each pressing block 2, and the two second fastening holes 5 on each fixing column 42 in sequence. The other clamping spring sheet 1 is set up similarly. Figure 2 As shown, the second fastening hole 5 is provided on the base 4.

[0062] For example, the second part 12 is provided with two through holes with a diameter of 2.2 mm, and the first part 11 is provided with two through holes with a diameter of 1.8 mm, which are respectively used to connect the base 4 and the pressure block 2. By fastening the bolts to install the clamping spring 1, the clamping spring 1 or the pressure block 2 can be easily installed, disassembled and replaced as needed, providing greater flexibility, so that components can be quickly adjusted or replaced as needed in different types of experiments.

[0063] In a preferred implementation extended from this embodiment, a circular biological sample circle 3 is generally used in the field of cryo-electron microscopy to store a frozen biological sample circle 3. Among them, a groove 21 is opened on one side of the two pressing blocks 2 forming the clamping space, and the length of the groove 21 is the same as the length of the pressing block 2, and the inner edge shape of the two grooves 21 matches the outer edge shape of the biological sample circle 3.

[0064] Specifically, Figure 3 As shown, Figure 3The schematic diagram of the structure of the pressing block 2 of the embodiment of the utility model. The side wall of the pressing block 2 forming the clamping space is provided with a groove 21. It is used in conjunction with the clamping spring 1. When the biological sample circle 3 is slowly fed in, the pressing block 2 enters the opening of the groove 21 under the extrusion force, allowing the biological sample circle 3 to slowly slide in, and finally ensures that the biological sample circle 3 is smoothly inserted and safely and reliably fixed, and there is no need to use an additional driving mechanism for clamping. It has a self-positioning function, and can automatically adjust the sample that is not fed in horizontally to make it horizontal and stable, thereby realizing the compression and fixation of the biological sample circle 3.

[0065] Specifically, the cross section of the groove 21 between the two relatively arranged pressing blocks 2 is tapered, that is, the width of the groove 21 gradually narrows in the direction away from the clamping space. The design of the tapered groove 21 can serve as a guide to help the operator more accurately align and insert the biological sample circle 3 into the correct position, thereby reducing operational errors. And because the groove 21 gradually becomes smaller and forms a cone, when the sample is inserted into the pressing block 2 and the pressing block 2 is stretched open, the groove gradually narrows, which can effectively guide the sample into the center position of the groove 21, that is, the end point of the cone, so that the annular outer edge of the biological sample circle 3 can abut against the groove 21, ensuring that the sample is correctly clamped and fixed.

[0066] In addition, the conical groove 21 can also adapt to biological sample circles 3 of different sizes and shapes. The wider mouth allows larger samples to be smoothly inserted, while the smaller mouth can effectively clamp and fix smaller samples. The length of the groove 21 is the same as the length of the pressing block 2, so that the biological sample circle 3 can be fixed within the distance formed by the opening on one side of the pressing block 2 to the opening on the opposite side.

[0067] In summary, the utility model is used to fix the biological sample circle 3, and does not need to use an additional driving mechanism for clamping. It has a self-positioning function, and can automatically adjust the sample that is not fed horizontally to make it horizontal and stable. For the biological sample circle 3 that is easy to fall off, it is firmly fixed and has a good imaging effect. It allows the sample to be prepared without strict preparation, which reduces the difficulty of preparation and the cost of sample preparation. The elastic clamping body is used for fitting fixation, which reduces the possibility of the clamping body scratching the sample surface. In addition, the center of the sample is suspended, which can protect the biological sample circle 3 from damage to the greatest extent. The fixing device provided by the utility model can significantly improve the efficiency and imaging quality of the cryo-electron microscope in the above-mentioned application scenarios, and promote scientific research and technological development in related fields such as structural biology, cell biology, virology, materials science, and clinical research.

[0068] It should be noted that the various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0069] It should also be noted that, in this article, the orientation or position relationship indicated by the terms "upper", "lower", "left", "right", "inside", "outside", etc. is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the utility model. In addition, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations, nor can they be understood as indicating or implying relative importance. Moreover, the term "include" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or terminal device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or terminal device.

[0070] The above is a detailed introduction to a fixing device for biological material analysis provided by the present application. The principles and implementation methods of the present application are described in detail using specific examples. The description of the above embodiments is only used to help understand the present application, and the content of this specification should not be understood as limiting the present application. At the same time, for those skilled in the art, according to the present application, there will be different forms of changes in the specific implementation methods and application scopes. It is not necessary and impossible to list all the implementation methods here, and the obvious changes or modifications derived therefrom are still within the protection scope of the present application.

Claims

1. A fixing device for biological material analysis, characterized in that: include: A base, used to be assembled to the cold stage of the electron microscope chamber; Two pressing blocks arranged opposite to each other form a clamping space, wherein the clamping space is configured to clamp a biological sample circle; A clamping spring piece connects the pressing block and the base; When the biological sample circle is sent into the clamping space, the clamping spring automatically adjusts the clamping force of the clamping space by using its own elastic force to horizontally clamp the biological sample circle.

2. A fixing device for biological material analysis according to claim 1, characterized in that: The two clamping springs are provided with two, and the two clamping springs are connected with the two pressing blocks in a one-to-one correspondence.

3. A fixing device for biological material analysis according to claim 1, characterized in that: The clamping spring is divided into a first part and a second part by a rectangular groove, the first part is fixed on the pressing block, and the second part is fixed on the base; Wherein, the first part is located above the second part.

4. A fixing device for biological material analysis according to claim 3, characterized in that: The rectangular groove extends from one end of the clamping spring sheet to the other opposite end and has a distance from the other end.

5. A fixing device for biological material analysis according to claim 3, characterized in that: The height of the rectangular groove is smaller than the heights of the first portion and the second portion, and the height of the first portion is the same as or different from the height of the second portion.

6. A fixing device for biological material analysis according to claim 1, characterized in that: The pressing block is in sliding contact with the base.

7. A fixing device for biological material analysis according to claim 1, characterized in that: The clamping spring pieces are detachably connected to the pressing block and the base respectively.

8. A fixing device for biological material analysis according to claim 7, characterized in that: The fixing device also includes: At least one fastening bolt, at least one first fastening hole is provided on the clamping spring piece, at least one second fastening hole is provided on the base and the pressing block respectively, each of the fastening bolts passes through the first fastening hole and the second fastening hole in sequence to connect the clamping spring piece with the base and the pressing block.

9. A fixing device for biological material analysis according to claim 1, characterized in that: A groove is provided on one side of the two pressing blocks forming the clamping space. The length of the groove is the same as the length of the pressing block, and the inner edge shape of the two grooves matches the outer edge shape of the biological sample circle.

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