Cell suspension refrigerating and shaking device
By designing a cell suspension refrigeration and shake device containing a semiconductor refrigeration structure and transmission mechanism, the problem of poor cell sinking and temperature control during cell suspension mixing is solved, and more uniform mixing and more stable temperature control are achieved, reducing the generation of condensate.
Patent Information
- Application Number
- CN202421264972.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-04
- Publication Date
- 2025-03-21
- Estimated Expiration
- 2034-06-04
AI Technical Summary
In the prior art, during the process of mixing and shaking of cell suspensions, the center of gravity remains unchanged, resulting in poor mixing effect of cell sinking and bottoming, inaccurate temperature control, and easy to produce condensate, affecting cell activity.
A cell suspension refrigeration shaker device including a base, a box, a semiconductor refrigeration structure, a power source, a transmission mechanism, a temperature sensor, a thermostat and a controller are designed. The temperature is detected by the temperature sensor and controlled by the semiconductor refrigeration structure. The power source and a transmission mechanism drive the box to rotate, causing the cell suspension bag to oscillate horizontally and large inclination angle.
Effectively avoid cells or precipitates in the liquid depositing at the bottom, mixing the liquid more evenly, improving mixing efficiency, and ensuring that the temperature is stable within the set range, while reducing the generation of condensate.
Smart Images
Figure CN222647941U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a cell suspension refrigeration and shaking device. Background Art
[0002] In biological application fields such as cell culture, the cell suspension needs to be mixed and shaken after packaging. Currently, most of the mixing is done by vertically hanging liquid bags and squeezing them horizontally. However, the center of gravity of this method does not change, resulting in poor mixing effect for cells that sink to the bottom. In addition, most of the existing devices use open-space open-loop refrigeration control, which results in inaccurate temperature control and easy generation of condensed water, and cannot maintain the activity of cells in the cell suspension.
[0003] Therefore, it is necessary to design a new device to effectively prevent cells or sediments in the liquid from settling at the bottom, mix the liquid more evenly, improve the mixing efficiency, ensure that the temperature is stable within the set range, and reduce the generation of condensed water. Utility Model Content
[0004] The utility model aims to overcome the defects of the prior art and provide a cell suspension refrigeration and shaking device.
[0005] To solve the above technical problems, the purpose of the utility model is achieved through the following technical solutions: to provide a cell suspension refrigeration and shaking device, including: a base, a box body, a semiconductor refrigeration structure, a power source, a transmission mechanism, a temperature sensor, a temperature controller and a controller, the temperature sensor is built into the box body, the temperature sensor is connected to the temperature controller; the temperature controller is connected to the semiconductor refrigeration structure; the transmission mechanism is connected to the semiconductor refrigeration structure; and the transmission mechanism is connected to the base; the box body is connected to the semiconductor refrigeration structure, the controller is connected to the power source; the power source is connected to the transmission mechanism, and a cell suspension bag placed parallel to the bottom of the box body is placed in the box body.
[0006] A further technical solution is as follows: a cavity for placing a cell suspension bag is provided in the box body; and the lower end surface of the box body is connected to the semiconductor refrigeration structure.
[0007] A further technical solution is that the side walls and the upper end surface of the cavity are respectively connected with heat insulating foam.
[0008] Its further technical solution is: the semiconductor refrigeration structure includes a shell with an opening at the upper end, a semiconductor refrigeration plate, a heat pipe, a heat sink and a fan; the semiconductor refrigeration plate, the heat pipe, the heat sink and the fan are respectively installed in the shell, and the opening of the shell is connected to the lower end surface of the box body.
[0009] Its further technical solution is: the transmission mechanism includes a first synchronous wheel, a synchronous belt, a rotating shaft and a second synchronous wheel, the power source includes an output shaft, the output shaft is connected to the first synchronous wheel; the second synchronous wheel is connected to the rotating shaft; the other end of the rotating shaft passes through the housing and is connected to the housing; the second synchronous wheel is connected to the first synchronous wheel through the synchronous belt.
[0010] A further technical solution is as follows: a left limit sensor and a right limit sensor are installed on the power source, and the left limit sensor and the right limit sensor are connected to the controller respectively.
[0011] A further technical solution is: the controller is connected to the power source via a driver.
[0012] A further technical solution is: a power input port is provided on the base.
[0013] A further technical solution is that the power input port is connected to the semiconductor refrigeration structure and the power source respectively.
[0014] Compared with the prior art, the utility model has the following beneficial effects: the utility model arranges a base, a box body, a semiconductor refrigeration structure, a power source, a transmission mechanism, a temperature sensor, a temperature controller and a controller, uses the temperature sensor to detect the temperature in the box body, and uses the semiconductor refrigeration structure to control the temperature. In addition, the power source and the transmission mechanism are used to drive the box body to rotate, so that the cell suspension bag can oscillate back and forth at a large horizontal angle; the cells or sediments in the liquid are effectively prevented from being deposited at the bottom, the liquid is mixed more evenly, the mixing efficiency is improved, and the temperature can be ensured to be stable within a set range, while reducing the generation of condensed water.
[0015] The utility model is further described below in conjunction with the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0017] Figure 1 A schematic diagram of the three-dimensional structure of a cell suspension refrigeration and shaking device provided in an embodiment of the utility model;
[0018] Figure 2 A schematic diagram of the side structure of a cell suspension cooling and shaking device provided in an embodiment of the utility model;
[0019] Figure 3 A schematic diagram of the explosion structure of a cell suspension refrigeration and shaking device provided in an embodiment of the utility model;
[0020] Figure 4 A schematic diagram of the exploded structure of the transmission mechanism provided in the embodiment of the utility model;
[0021] Description of the symbols in the figure:
[0022] 10. Base; 20. Box body; 30. Semiconductor refrigeration structure; 40. Power source; 50. Transmission mechanism; 51. First synchronous wheel; 52. Synchronous belt; 53. Rotating shaft; 54. Second synchronous wheel; 60. Temperature controller; 70. Controller; 80. Power input port. DETAILED DESCRIPTION
[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0024] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.
[0025] It should also be understood that the terms used in this utility model specification are only for the purpose of describing specific embodiments and are not intended to limit the utility model. As used in this utility model specification and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.
[0026] It should be further understood that the term “and / or” used in the present specification and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.
[0027] See also Figure 1 , Figure 1 A three-dimensional structural schematic diagram of a cell suspension refrigeration and shaking device provided in an embodiment of the utility model can be used in the scenario of cell suspension subpackaging and shaking, so as to effectively prevent cells or sediments in the liquid from settling at the bottom, mix the liquid more evenly, improve the mixing efficiency, and ensure that the temperature is stable within the set range, while reducing the generation of condensed water.
[0028] See also Figures 1 to 3 A cell suspension refrigeration and shaking device is characterized in that it includes: a base 10, a box body 20, a semiconductor refrigeration structure 30, a power source 40, a transmission mechanism 50, a temperature sensor, a temperature controller 60 and a controller 70, the temperature sensor is built in the box body 20, and the temperature sensor is connected to the temperature controller 60; the temperature controller 60 is connected to the semiconductor refrigeration structure 30; the transmission mechanism 50 is connected to the semiconductor refrigeration structure 30; and the transmission mechanism 50 is connected to the base 10; the box body 20 is connected to the semiconductor refrigeration structure 30, and the controller 70 is connected to the power source 40; the power source 40 is connected to the transmission mechanism 50, and a cell suspension bag is placed in the box body 20 parallel to the bottom of the box body 20.
[0029] In this embodiment, when the device is powered on, the temperature sensor will monitor the temperature inside the box body 20. If the temperature is higher than the preset temperature, the temperature sensor will trigger the relay to output a signal to the thermostat 60, and the thermostat 60 will drive the power supply to transmit power to the semiconductor refrigeration structure 30. After the semiconductor refrigeration structure 30 is powered on, it uses the movement of electrons to transfer heat from one side to the other side; then, the heat is dissipated through the heat pipe and the cooling fan to achieve a cooling effect. If the temperature is equal to the preset temperature, the temperature sensor will disconnect the relay output signal, and under the drive of the thermostat 60, the power supply will disconnect the power supply to the semiconductor refrigeration structure 30, causing the semiconductor refrigeration structure 30 to stop working to maintain the device at the target temperature level.
[0030] See also Figure 2 , by default, the box body 20 will turn to the right limit position. The preset mixing program can be run by pressing the button on the controller 70. The controller 70 will continue to send a left turn command to the driver, and the driver will continue to send a pulse signal to the power source 40 to make the power source 40 turn left until the left limit sensor detects a signal. Then, the controller 70 will continue to send a right turn command to the driver, and the driver will continue to send a pulse signal to the power source 40 to make the power source 40 turn right until the right limit sensor detects a signal. Such a reciprocating cycle allows the mixing movement of the cell suspension in the cell suspension bag in the box body 20 to continue.
[0031] The liquid bag is horizontally reciprocated at a large inclination angle (±30°) and the center of gravity is reciprocated back and forth, which can effectively avoid sinking to the bottom and ensure the density consistency of the packaging; in addition, the cell suspension bag is placed in a closed box body 20 for closed-loop control, and the temperature control is accurate, which can well maintain cell activity during large-volume and long-term packaging.
[0032] In one embodiment, see Figure 3The box body 20 is provided with a cavity for placing a cell suspension bag; the lower end surface of the box body 20 is connected to the semiconductor refrigeration structure 30.
[0033] In one embodiment, the side walls and the upper end surface of the cavity are respectively connected with thermal insulation foam.
[0034] In this embodiment, the top surface of the box body 20 is a door panel, which is provided with a handle for opening the door panel. Insulating foam is attached between the other surfaces and the top surface to reduce the heat exchange between the box body 20 and the outside world, thereby preventing the generation of condensed water and affecting cell activity.
[0035] In one embodiment, the semiconductor refrigeration structure 30 includes a shell with an opening at the upper end, a semiconductor refrigeration plate, a heat pipe, a heat sink and a fan; the semiconductor refrigeration plate, the heat pipe, the heat sink and the fan are respectively installed in the shell, and the opening of the shell is connected to the lower end surface of the box body 20.
[0036] The housing is fixed on the base 10 to ensure the stability and sealing of the housing. The housing is usually the outer shell of the device, which is used to protect the internal components and provide external support.
[0037] The semiconductor cooling chip is an important component for providing cooling effect. Generally, the semiconductor cooling chip should be installed at a suitable position inside the shell to ensure that it can effectively reduce the temperature. The specific installation position is close to the opening, close to the lower end surface of the box body 20.
[0038] The function of the heat pipe is to transfer heat from the heat source to the heat sink and fan for heat dissipation. The heat pipe is installed between the semiconductor cooling fin and the heat sink to ensure that the heat can be effectively transferred to the cooling system.
[0039] The heat sink is installed at the end of the heat pipe to diffuse and dissipate the heat. The heat sink is usually designed with a large area and good heat dissipation performance to ensure that the heat is effectively transferred to the surrounding environment.
[0040] The fan is installed near the heat sink to accelerate air flow and enhance heat dissipation. The fan can be fixed directly to the heat sink or installed on a bracket to provide better air circulation.
[0041] In one embodiment, see Figure 4 The transmission mechanism 50 includes a first synchronous wheel 51, a synchronous belt 52, a rotating shaft 53 and a second synchronous wheel 54. The power source 40 includes an output shaft, which is connected to the first synchronous wheel 51; the second synchronous wheel 54 is connected to the rotating shaft 53; the other end of the rotating shaft 53 passes through the shell and is connected to the shell; the second synchronous wheel 54 is connected to the first synchronous wheel 51 through the synchronous belt 52.
[0042] The transmission mechanism 50 further includes a housing, in which the first synchronous wheel 51 , the synchronous belt 52 and the second synchronous wheel 54 are built.
[0043] When the power source 40 (output shaft) provides power, the first synchronous wheel 51 is driven to rotate by the rotation of the output shaft. The synchronous belt 52 transmits the movement of the first synchronous wheel 51 to the second synchronous wheel 54, thereby driving the rotating shaft 53 to rotate. The other end of the rotating shaft 53 passes through the shell and is connected to the shell, so when the rotating shaft 53 rotates, the shell will also rotate, and when the shell rotates, the box body 20 will also rotate, so that the cell suspension in the box body 20 is shaken evenly.
[0044] Specifically, the other end of the rotating shaft 53 is connected to the housing through a bearing, ensuring that the rotating shaft 53 can rotate freely inside the housing while maintaining the structural stability of the housing. The bearing can reduce the friction between the rotating shaft 53 and the housing and support the rotation of the rotating shaft 53, thereby achieving smooth movement. Overall, such a design enables the output of the power source 40 to drive the housing to rotate through the transmission mechanism 50 to complete the corresponding functional operation.
[0045] In one embodiment, a left limit sensor and a right limit sensor are installed on the power source 40 , and the left limit sensor and the right limit sensor are connected to the controller 70 , respectively.
[0046] In this embodiment, the left limit sensor and the right limit sensor are mainly limit switches.
[0047] The left limit sensor and the right limit sensor are installed on the power source 40, and their function is to detect the rotation position of the power source 40. Specifically, when the power source 40 (which may be the output shaft) rotates to a specific position, the left limit sensor and the right limit sensor will detect this position and send a signal to the controller 70. For example, when the power source 40 rotates to the left limit position, the left limit sensor will detect this position and send a signal to the controller 70 to instruct the controller 70 to stop or change the movement direction of the power source 40. Similarly, when the power source 40 rotates to the right limit position, the right limit sensor will send a signal to the controller 70 to instruct the controller 70 to stop or change the movement direction of the power source 40.
[0048] In this way, through the position detection of the left limit sensor and the right limit sensor, the controller 70 can ensure that the power source 40 moves within a specific range and avoids exceeding the set limit position.
[0049] In one embodiment, the controller 70 is connected to the power source 40 via a driver.
[0050] In one embodiment, see Figure 1 The base 10 is provided with a power input port 80 .
[0051] In one embodiment, see Figure 1 The power input port 80 is connected to the semiconductor refrigeration structure 30 and the power source 40 respectively.
[0052] In this embodiment, the power source 40 includes a stepping motor.
[0053] The above-mentioned cell suspension refrigeration and shaking device is provided with a base 10, a box body 20, a semiconductor refrigeration structure 30, a power source 40, a transmission mechanism 50, a temperature sensor, a temperature controller 60 and a controller 70. The temperature in the box body 20 is detected by the temperature sensor, and the temperature is controlled by the semiconductor refrigeration structure 30. In addition, the power source 40 and the transmission mechanism 50 are used to drive the box body 20 to rotate, so that the cell suspension bag is horizontally reciprocated at a large inclination angle. This effectively prevents cells or sediments in the liquid from being deposited at the bottom, mixes the liquid more evenly, improves the mixing efficiency, ensures that the temperature is stable within a set range, and reduces the generation of condensed water.
[0054] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the utility model, and these modifications or replacements should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.
Claims
1. A cell suspension refrigeration and shaking device, characterized in that: include: A base, a box body, a semiconductor refrigeration structure, a power source, a transmission mechanism, a temperature sensor, a temperature controller and a controller, wherein the temperature sensor is built into the box body, and the temperature sensor is connected to the temperature controller; the temperature controller is connected to the semiconductor refrigeration structure; the transmission mechanism is connected to the semiconductor refrigeration structure; and the transmission mechanism is connected to the base; the box body is connected to the semiconductor refrigeration structure, and the controller is connected to the power source; the power source is connected to the transmission mechanism, and a cell suspension bag is placed in the box body parallel to the bottom of the box body.
2. A cell suspension refrigeration and shaking device according to claim 1, characterized in that: The box body is provided with a cavity for placing a cell suspension bag; the lower end surface of the box body is connected to the semiconductor refrigeration structure.
3. A cell suspension refrigeration and shaking device according to claim 2, characterized in that: The side walls and the upper end surface of the cavity are respectively connected with heat-insulating foam.
4. A cell suspension refrigeration and shaking device according to claim 3, characterized in that: The semiconductor refrigeration structure includes a shell with an opening at the upper end, a semiconductor refrigeration plate, a heat pipe, a heat sink and a fan; the semiconductor refrigeration plate, the heat pipe, the heat sink and the fan are respectively installed in the shell, and the opening of the shell is connected to the lower end surface of the box body.
5. A cell suspension refrigeration and shaking device according to claim 4, characterized in that: The transmission mechanism includes a first synchronous wheel, a synchronous belt, a rotating shaft and a second synchronous wheel. The power source includes an output shaft, which is connected to the first synchronous wheel; the second synchronous wheel is connected to the rotating shaft; the other end of the rotating shaft passes through the housing and is connected to the housing; the second synchronous wheel is connected to the first synchronous wheel through the synchronous belt.
6. A cell suspension refrigeration and shaking device according to claim 5, characterized in that: A left limit sensor and a right limit sensor are installed on the power source, and the left limit sensor and the right limit sensor are connected to the controller respectively.
7. The cell suspension refrigeration and shaking device according to claim 1, characterized in that: The controller is connected to the power source through a driver.
8. The cell suspension refrigeration and shaking device according to claim 1, characterized in that: The base is provided with a power input port.
9. The cell suspension refrigeration and shaking device according to claim 8, characterized in that: The power input port is connected to the semiconductor refrigeration structure and the power source respectively.