Stem cell culture medium filtering device

Abstract

The utility model discloses a stem cell culture medium filtering device, and relates to the technical field of culture medium filtering, the stem cell culture medium filtering device comprises a barrel body, the top of the barrel body is provided with two first limiting grooves, each first limiting groove is provided with a protruding block, the top of each protruding block is fixedly connected with a cup body, the barrel body is provided with a second limiting groove, and each second limiting groove is fixedly connected with a cup body. A second limiting groove is formed in the barrel body, a push rod is arranged in the second limiting groove, a rotating ring is fixedly connected to the bottom of the push rod, a plurality of third limiting grooves are formed in the rotating ring, a screen ring is arranged in each third limiting groove, a cell screen is fixedly connected to the side wall of the upper end of the end, away from the barrel body, of each screen ring, and a heating mechanism is fixedly connected to the outer side wall of the barrel body. By arranging the screen ring, the third limiting groove, the rotating ring and the push rod, a film formed by surface tension of a culture medium among screen holes is broken under the driving of back-and-forth rotation, so that the blockage of the cell screen is relieved, and the filtering effect of the culture medium is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of culture medium filtration, in particular to a stem cell culture medium filtration device. Background Art

[0002] During the cell culture process, cells secrete active substances such as cytokines into the culture medium. The culture medium containing cell secretions is called conditioned culture medium. Stem cells have multidirectional differentiation and self-renewal capabilities. They are widely present in tissues such as bone marrow, umbilical cord, peripheral blood and fat. They can be used to repair or replace damaged and diseased tissues and organs. However, in addition to beneficial components such as cytokines, stem cell culture medium also contains harmful substances such as waste products of cell growth and metabolism, dead cells and cell fragments, which need to be filtered before use.

[0003] Cell sieves are generally used for filtration. Since cells are very small, the pores of the cell sieves are also very small. At the same time, due to the surface tension of the culture medium itself, a film is easily formed on the filter. The film and harmful substances can easily cause the filter to be blocked, affecting the filtration effect. Utility Model Content

[0004] The utility model aims to solve the problems in the background technology and proposes a stem cell culture medium filtering device.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A stem cell culture medium filtering device comprises a barrel body, wherein two first limiting grooves are provided on the top of the barrel body, the first limiting grooves are provided with a protrusion, the top of the protrusion is fixedly connected to a cup body, the barrel body is provided with a second limiting groove, the second limiting groove is provided with a push rod, the bottom of the push rod is fixedly connected to a rotating ring, a plurality of third limiting grooves are provided on the rotating ring, the third limiting grooves are provided with a screen ring, a cell screen is fixedly connected to the side wall of the upper end of the screen ring away from the barrel body, and a heating mechanism is fixedly connected to the outer wall of the barrel body.

[0007] Preferably, the heating mechanism comprises a heating shell fixedly connected to the outer side wall of the barrel body, the heating shell is provided with a cavity, and the cavity is provided with a heating pipe.

[0008] Preferably, a liquid discharge port is provided at the bottom of the barrel body, and a sealing cover is threadedly connected to the outer wall of the liquid discharge port.

[0009] Preferably, the push rod is divided into two parts, and the upper half of the push rod is threadedly connected to the lower half.

[0010] Preferably, the rotating ring is slidably connected to the barrel body, and the top of the second limiting groove is provided with an opening on the side wall of the cup body.

[0011] Preferably, a liquid inlet is provided on the top of the cup body.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. The utility model provides a screen ring, a third limiting groove, a rotating ring and a push rod, which can cause the thin film formed between the screen holes by the surface tension of the culture medium itself to rupture under the drive of back and forth rotation, thereby slowing down the blockage of the cell screen and improving the filtering effect of the culture medium.

[0014] 2. The utility model provides the first limiting groove and the protrusion, so that when the surface of the cell screen is blocked by harmful substances, the cell screen can be replaced to facilitate cleaning.

[0015] 3. The utility model adjusts the temperature of different types of stem cell culture media after filtration by providing a heating shell and a heating tube, so as to maintain the performance of the culture media and the activity of the stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of a stem cell culture medium filtration device proposed by the utility model;

[0017] Figure 2 This is a schematic diagram of a front cross-sectional structure of a stem cell culture medium filtration device proposed by the utility model;

[0018] Figure 3 This is a schematic diagram of a left-side sectional structure of a stem cell culture medium filtration device proposed by the utility model;

[0019] Figure 4 This is a schematic diagram of a top view and cross-sectional structure of a cell screen of a stem cell culture medium filtration device proposed by the utility model;

[0020] Figure 5 for Figure 2 A schematic diagram of the enlarged structure at point A;

[0021] Figure 6 for Figure 4 A schematic diagram of the enlarged structure at B;

[0022] Figure 7 for Figure 2 A schematic diagram of the enlarged structure at C;

[0023] Figure 8 for Figure 3 Enlarged structural diagram at D.

[0024] In the figure: 1 barrel body, 2 cup body, 3 rotating ring, 4 cell screen, 5 heating shell, 6 heating tube, 7 cavity, 8 liquid inlet, 9 liquid discharge port, 10 sealing cover, 11 second limiting groove, 12 screen ring, 13 push rod, 14 third limiting groove, 15 protrusion, 16 first limiting groove. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0026] Reference Figure 1-8 , a stem cell culture medium filtering device, comprising a barrel body 1, the barrel body 1 is composed of a large cylinder, a small cylinder and a cone, and the cone, the small cylinder and the large cylinder are connected, a circular opening is opened on the top of the barrel body 1, and two first limiting grooves 16 are opened on the top of the side wall of the barrel body 1, the first limiting grooves 16 are arc-shaped, and the width of the first limiting grooves 16 does not exceed the thickness of the side wall of the barrel body 1, the first limiting grooves 16 are provided with protrusions 15, the protrusions 15 are arc-shaped, and the protrusions 15 and the first limiting grooves 16 are abutted, and the top of the protrusions 15 is fixedly connected with a cup body 2, the cup body 2 is composed of two cones and a cylinder, and the two cones and the cylinder are connected, a circular opening is opened at the bottom of the cup body 2, and the opening of the barrel body 1 is consistent in size with the opening of the cup body 2;

[0027] A second limiting groove 11 is provided on the barrel body 1. The second limiting groove 11 is arc-shaped, and the bottom of the second limiting groove 11 passes through the inner wall of the barrel body 1. A push rod 13 is provided on the second limiting groove 11. A rotating ring 3 is fixedly connected to the bottom of the push rod 13. A part of the rotating ring 3 is in the second limiting groove 11, and a part exceeds the inner wall of the barrel body 1. A plurality of third limiting grooves 14 are provided on the top of the end of the rotating ring 3 away from the bottom of the push rod 13. A screen ring 12 is provided on the third limiting groove 14. The screen ring 12 is composed of a ring and a plurality of protrusions fixedly connected to the bottom of the ring. The protrusions at the bottom of the ring are against the third limiting groove 14. A cell screen 4 is fixedly connected to the side wall of the upper end of the screen ring 12 away from the barrel body 1, and a heating mechanism is fixedly connected to the outer side wall of the lower end of the barrel body 1.

[0028] The heating mechanism includes a heating shell 5 fixedly connected to the outer wall of the lower end of the barrel body 1, the heating shell 5 is composed of a circle and a cone-shaped ring, the heating shell 5 is provided with a cavity 7, the cavity 7 is provided with a heating tube 6, the heating tube 6 is in a threaded shape, a discharge port 9 is provided at the bottom of the barrel body 1, a sealing cover 10 is threadedly connected to the outer wall of the discharge port 9, the sealing cover 10 is similar to the shape of a bottle cap, the push rod 13 is divided into two, and the upper half of the push rod 13 is threadedly connected to the lower half, the rotating ring 3 is slidably connected to the barrel body 1, because the push rod 13 is limited by the second limiting groove 11, the amplitude of the sliding connection between the rotating ring 3 and the barrel body 1 is the same as the amplitude of pushing the push rod 13, the top of the second limiting groove 11 is provided with an opening on the side wall of the cup body 2, and the top of the cup body 2 is provided with a liquid inlet 8, and the liquid inlet 8 is circular.

[0029] The detailed working process of the utility model is as follows:

[0030] First, the culture medium is poured into the liquid inlet 8, and then the culture medium will flow along the cup body 2 and the barrel body 1 to the cell screen 4, and then the push rod 13 is grasped and moved left and right to drive the rotating ring 3, the screen ring 12 and the cell screen 4 to rotate left and right, and then the heating tube 6 is started to adjust the temperature to the temperature required by the stem cells. After the culture medium is filtered, the sealing cover 10 is rotated to open and the culture medium flows out from the discharge port 9.

[0031] When the cell screen 4 needs to be replaced, the upper part of the push rod 13 is rotated to separate the push rod 13, and then the cup body 2 is moved upward, and then the screen ring 12 is taken out from the third limiting groove 14, and the cell screen 4 is cleaned and the screen ring 12 is placed in the third limiting groove 14.

[0032] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A stem cell culture medium filtration device, comprising a barrel (1), characterized in that: The barrel body (1) is provided with two first limiting grooves (16) on the top, the first limiting grooves (16) are provided with a protrusion (15), the top of the protrusion (15) is fixedly connected to the cup body (2), the barrel body (1) is provided with a second limiting groove (11), the second limiting groove (11) is provided with a push rod (13), the bottom of the push rod (13) is fixedly connected to a rotating ring (3), the rotating ring (3) is provided with a plurality of third limiting grooves (14), the third limiting grooves (14) are provided with a screen ring (12), the upper side wall of the screen ring (12) away from the barrel body (1) is fixedly connected to a cell screen (4), and the outer side wall of the barrel body (1) is fixedly connected to a heating mechanism.

2. A stem cell culture medium filtration device according to claim 1, characterized in that: The heating mechanism comprises a heating shell (5) fixedly connected to the outer wall of the barrel (1), the heating shell (5) being provided with a cavity (7), and the cavity (7) being provided with a heating tube (6).

3. A stem cell culture medium filtration device according to claim 1, characterized in that: The bottom of the barrel body (1) is provided with a liquid discharge port (9), and a sealing cover (10) is threadedly connected to the outer wall of the liquid discharge port (9).

4. A stem cell culture medium filtration device according to claim 1, characterized in that: The push rod (13) is divided into two parts, and the upper part of the push rod (13) is threadedly connected to the lower part.

5. The stem cell culture medium filtration device according to claim 1, characterized in that: The rotating ring (3) is slidably connected to the barrel body (1), and the top of the second limiting groove (11) is provided with an opening on the side wall of the cup body (2).

6. The stem cell culture medium filtration device according to claim 1, characterized in that: The top of the cup body (2) is provided with a liquid inlet (8).

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