Reagent cooling protection device
By designing a reagent cooling protection device, using the combination of cooling module and condensate barrier module, the problems of condensate formation and PCR plate lifting during reagent cooling during genetic testing are solved, and the accuracy and safety of reagents are achieved.
Patent Information
- Application Number
- CN202421335915.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-12
- Publication Date
- 2025-03-21
- Estimated Expiration
- 2034-06-12
AI Technical Summary
During the genetic testing process, temperature changes in the reagent during heating and cooling lead to the formation of condensate water, affecting the accuracy of the test. At the same time, the prior art may cause the PCR plate to be lifted by directly placing the cover plate, affecting the safety of the reagent.
A reagent cooling protection device is designed, including a cooling module and a condensate barrier module. The cooling module achieves rapid cooling through semiconductor refrigeration sheets and cooling water drains. The condensate barrier module isolates the PCR plate from the air through a support frame, rotating shaft, elastic parts and flexible cover plate to prevent condensation water from forming, and fixes the PCR plate through jaws to prevent lifting.
It effectively prevents the formation of condensate and ensures the accuracy of the reagent. At the same time, by fixing the PCR plate, the safety of the reagent is ensured and the risk of lifting the PCR plate is avoided.
Smart Images
Figure CN222651771U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of reagent detection, in particular to a reagent temperature reduction and cooling protection device. Background Art
[0002] With the continuous improvement of medical technology, there are abundant equipment to meet the actual needs of gene testing (medical, cancer, etc.). And the steps of gene testing are relatively rigorous and cumbersome. For example, the tip of the gun needs to be positioned to drip the fluorescent agent, and the subsequent heating and cooling steps need to be strictly followed.
[0003] Among them, after the reagent is heated to a certain temperature within a specified time, it also needs to be cooled within a specified time to achieve low-temperature storage. This process may cause the temperature to fluctuate by 60-70°C, which will cause condensed water to condense on the reagent-carrying PCR plate, thereby affecting the accuracy of the test. The existing technology often uses a cover plate directly placed on the PCR plate, but this behavior may cause the PCR plate to be lifted from the cooling device, thereby affecting the safety of the reagent. Utility Model Content
[0004] The utility model provides a reagent cooling protection device, which aims to effectively prevent a PCR plate from being lifted while cooling the reagent for low-temperature storage, thereby ensuring safe use of the reagent.
[0005] In a first aspect, an embodiment of the utility model provides a reagent cooling protection device, comprising:
[0006] The cooling module includes a cooling base and a cooling water row detachably placed on the cooling base, the cooling water row is used to cool the reagent; the cooling base is provided with a fixing unit;
[0007] The fixing unit comprises a clamping claw for fixing the PCR plate, the end of the clamping claw is connected to a connecting rod through a connecting shaft, the end of the connecting rod is rotatably connected to a rotating wheel, and the rotating wheel is used to rotate under the drive of an external motor and drive the clamping claw to rotate.
[0008] The condensation water barrier module includes a support frame arranged parallel to the cooling base, and a rotating shaft connected to the first cover plate is provided on the top of the support frame; the first cover plate is fixedly connected to the flexible cover plate through an elastic member, so that the flexible cover plate can seal and cover the PCR plate, and the support frame is fixedly connected to a driving unit for driving the rotating shaft to rotate.
[0009] Optionally, the cooling module further includes a water tank and a heat dissipation water drain connected to a liquid outlet of the water tank, the liquid outlet of the heat dissipation water drain is connected to a cooling water drain, and the liquid outlet of the cooling water drain is connected to the water tank.
[0010] Optionally, the heat dissipation water row is provided with a heat dissipation pipeline.
[0011] Optionally, the cooling water drain is provided with a condensation water drain hole penetrating in a vertical direction.
[0012] Optionally, the cooling module further includes a semiconductor refrigeration sheet, which is arranged on the top of the cooling water row.
[0013] Optionally, temperature sensors are provided on both sides of the semiconductor refrigeration plate.
[0014] Optionally, the driving unit is a motor;
[0015] The output shaft of the motor extends to one side of the outside of the support frame and is connected to a first synchronous wheel, and the first synchronous wheel is connected to a second synchronous wheel at one end of the rotating shaft through a synchronous belt.
[0016] Optionally, the elastic member is a telescopic pin.
[0017] The embodiment of the utility model provides a reagent cooling protection device, which arranges the cooling module and the condensation water barrier module in parallel. Therefore, when the PCR plate carrying the reagent is placed on the cooling water drain, the cooling process is accompanied by the condensation water barrier module to isolate the PCR plate and the reagent from the air, thereby preventing the formation of condensation water. At the same time, due to the addition of a clamping claw for the PCR plate, the PCR plate is fixed in the process of covering and lifting the PCR plate by the condensation water barrier module, thereby ensuring the safety of the transportation of the reagent. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 A side view of a reagent cooling protection device provided by an embodiment of the utility model;
[0019] Figure 2 A schematic diagram of the structure of a cooling module in a reagent cooling protection device provided in an embodiment of the utility model;
[0020] Figure 3 A schematic diagram of the structure of a fixing unit in a reagent cooling protection device provided in an embodiment of the utility model;
[0021] Figure 4 It is a schematic structural diagram of a cooling water row in a reagent cooling protection device provided by an embodiment of the utility model;
[0022] Figure 5 It is a structural schematic diagram of a condensation water barrier module in a reagent cooling protection device provided in an embodiment of the utility model.
[0023] In the figure: 51, cooling module; 52, cooling base; 53, cooling water drain; 54, fixing unit; 55, PCR plate; 56, claw; 57, connecting rod; 58, rotating wheel; 59, condensation water barrier module; 510, supporting frame; 511, first cover plate; 512, rotating shaft; 513, elastic member; 514, flexible cover plate; 515, driving unit; 516, water tank; 517, cooling water drain; 518, condensation water drain hole; 519, semiconductor refrigeration plate; 520, first synchronous wheel; 521, second synchronous wheel; 522, synchronous belt. DETAILED DESCRIPTION
[0024] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It should also be noted that, for ease of description, only the parts related to the present invention, rather than all structures, are shown in the accompanying drawings.
[0025] Currently, genetic testing reagents can be stored at low temperatures by cooling them down, with the temperature range being 60-70°C. This will cause condensation to form above the cooling device and on the PCR plate that carries the reagents. If the condensation flows into the reagents, it will affect the accuracy of the test. Therefore, a cover is often placed directly on the PCR plate. However, this may cause the PCR plate to be lifted from the cooling device, thereby affecting the safety of the reagents.
[0026] Embodiment 1
[0027] In view of the above shortcomings, the utility model proposes a reagent cooling protection device, such as Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown, including:
[0028] The cooling module 51 includes a cooling base 52 (insulation plate) and a cooling water row 53 detachably placed on the cooling base 52, and the cooling water row 53 is used to cool the reagent; the cooling module 51 also includes a semiconductor cooling sheet 519, which is arranged on the top of the cooling water row 53; the cooling water row 53 cools the semiconductor cooling sheet 519; the cooling base 52 is fixed to the ground by a bracket, and the bracket is usually made of metal material to avoid affecting the service life during high temperature and cooling process;
[0029] The temperature span of the reagent during the cooling process is between 60-70°C, so the cooling water row 53 preferably adopts liquid cooling. The cooling module 51 also includes a water tank 516 and a heat dissipation water row 517 connected to the liquid outlet of the water tank 516. A water pump can be used in the water tank 516 as a power supply for water circulation. The liquid outlet of the heat dissipation water row 517 is connected to the cooling water row 53, and the liquid outlet of the cooling water row 53 is connected to the water tank 516. In addition, the heat dissipation water row 517 is provided with a heat dissipation pipeline; when the water pump transports the liquid to the heat dissipation water row 517, the heat in the liquid (coolant) will be released through the aforementioned heat dissipation pipeline; the liquid after heat dissipation will be re-input into the cooling water row 53. The cooling water row 53 is made of aluminum material, which has good metal thermal conductivity.
[0030] The main function of the semiconductor cooling sheet 519 is to increase the cooling speed of the reagent. At the same time, during the condensation and cooling process of the reagent, condensed water is easily generated on the surface of the heat insulation board. Therefore, it is necessary to provide a condensed water drainage hole 518 that runs vertically through the cooling water row 53. When condensed water is generated, it will be discharged downward through the condensed water drainage hole 518. And when the temperature of the reagent is cooled to the target temperature by the liquid, the liquid will be drawn out from the cooling water row 53 and transported back to the water pump.
[0031] In order to monitor the temperature drop of the reagent, temperature sensors need to be provided on the upper and lower surfaces of the semiconductor refrigeration plate 519 respectively. The temperature sensor on the upper surface is used to detect whether the reagent has been cooled to 4°C, and the temperature sensor on the lower surface is used to detect whether the temperature is too high.
[0032] Meanwhile, the cooling base 52 is provided with a fixing unit 54;
[0033] The fixing unit 54 includes a clamping claw 56 for fixing the PCR plate 55, the end of the clamping claw 56 is connected to the connecting rod 57 through a connecting shaft, and the end of the connecting rod 57 is rotatably connected to a rotating wheel 58, which is used to rotate under the drive of an external motor and drive the clamping claw 56 to rotate, so as to clamp or release the PCR plate 55. The above-mentioned connecting rod 57 adopts a structure arranged on both sides of the PCR plate 55. Specifically, the connecting rod 57 is arranged in parallel on both sides of the top of the cooling base 52 and can rotate around itself as an axis. The two ends of each connecting rod 57 are respectively provided with a clamping claw 56. When the connecting rod 57 rotates, the clamping claw 56 can be driven to buckle the cooling water row 53 to prevent the cooling water row 53 from being lifted up in the process of clamping the PCR plate 55.
[0034] The condensation water barrier module 59 includes a support frame 510 arranged parallel to the cooling base 52, and a rotating shaft 512 connected to the first cover plate 511 is provided on the top of the support frame 510; the first cover plate 511 is fixedly connected to the flexible cover plate 514 through an elastic member 513, so as to make the flexible cover plate 514 seal and cover the PCR plate 55. In a preferred embodiment, the above-mentioned flexible cover plate 514 adopts a shape matching the PCR plate 55. At the same time, the fixed connection method between the rotating shaft 512 and the first cover plate 511 includes but is not limited to the use of screw punching. The elastic member 513 used between the first cover plate 511 and the flexible cover plate 514 is to absorb the additional force in the process of the flexible cover plate 514 covering the PCR plate 55, thereby achieving the purpose of isolating the PCR plate 55 from the air.
[0035] The driving unit 515 preferably adopts a motor, such as a servo motor or a stepper motor, so that the rotation distance of the flexible cover plate 514 is more accurate. The motor is fixed on the support frame 510, and the output shaft of the motor extends to one side of the outside of the support frame 510 and is connected to the first synchronous wheel 520, and the first synchronous wheel 520 is connected to the second synchronous wheel 521 at one end of the rotating shaft 512 through a synchronous belt 522. The bottom of the support frame 510 is provided with a fixed rectangular hole, which is fixed to the bottom of the reagent detection device by bolts, and the support frame 510 can adjust its position within the range of the rectangular hole to meet the requirement of being parallel to the cooling base 52. In the process of motor driving, the first synchronous wheel 20 is the driving wheel, and the second synchronous wheel 21 is the driven wheel, which drives the first cover plate 511 and the flexible cover plate 514 to rotate around the rotating shaft 512, and the rotation angle is at least greater than 90°, preferably 90°-135°, so as to reduce the obstruction caused by the clamping claws of the reagent cooling device when clamping the PCR plate 55, and the side of the support frame 510 is also provided with a rectangular hole for moving the motor.
[0036] The embodiment of the utility model provides a reagent cooling protection device, which arranges the cooling module and the condensation water barrier module in parallel. Therefore, when the PCR plate carrying the reagent is placed on the cooling water drain, the cooling process is accompanied by the condensation water barrier module to isolate the PCR plate and the reagent from the air, thereby preventing the formation of condensation water. At the same time, due to the addition of a clamping claw for the PCR plate, the PCR plate is fixed in the process of covering and lifting the PCR plate by the condensation water barrier module, thereby ensuring the safety of the transportation of the reagent.
[0037] Embodiment 2
[0038] Based on the first embodiment, the working process of the above reagent cooling protection device is as follows:
[0039] First, the PCR plate 55 is placed on the cooling water row 53 of the cooling module 51 under the support of the clamp of the reagent cooling device. Before that, a semiconductor cooling sheet 519 is placed on the cooling water row 53; and the water tank 516, the heat dissipation water row 517 and the cooling water row 53 in the cooling module 51 are connected to each other to realize the circulation of liquid (coolant);
[0040] After that, the water pump in the water tank 516 is started to circulate the liquid. At this time, the temperature of the reagents on the PCR plate 55 drops by 60-70°C under the action of the coolant. At the same time, the temperature sensors on both sides of the surface of the semiconductor cooling sheet 519 are used to detect whether the temperature difference meets the drop requirement.
[0041] At the same time, during the temperature drop process, the driving unit 515 (motor) drives the first cover plate 511 to rotate, so that the flexible cover plate 514 is sealed and covers the PCR plate 55, and in order to prevent the flexible cover plate 514 from exerting too much force on the PCR plate 55, a telescopic pin is used between the first cover plate 511 and the flexible cover plate 514 as a force release device;
[0042] When condensed water appears during cooling and condensation, the condensed water will flow out of the cooling water drain 53 through the condensed water drain hole 18. At the same time, due to the sealed connection between the PCR plate 55 and the flexible cover plate 514, the condensed water in the reagent is prevented from condensing, and the condensed water in the air is prevented from condensing and flowing into the reagent.
[0043] Finally, the driving unit 515 drives the first cover plate 11 to drive the flexible cover plate 514 to open, and the clamping claws of the reagent cooling device clamp the PCR plate 55 and move it to other positions.
[0044] The embodiment of the utility model discloses the working process of the reagent cooling protection device on the basis of the first embodiment, and has the same technical effect, which will not be repeated here.
[0045] Some directional words are defined in the present utility model. Unless otherwise specified, the directional words used, such as "up", "down", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute limitations on the protection scope of the present utility model.
[0046] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.
[0047] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
Claims
1. A reagent cooling protection device, characterized in that: include: A cooling module (51) comprises a cooling base (52) and a cooling water row (53) detachably placed above the cooling base (52), wherein the cooling water row (53) is used to cool the reagent; the cooling base (52) is provided with a fixing unit (54); The fixing unit (54) comprises a clamping claw (56) for fixing the PCR plate (55), the end of the clamping claw (56) is connected to a connecting rod (57) via a connecting shaft, the end of the connecting rod (57) is rotatably connected to a rotating wheel (58), and the rotating wheel (58) is used to rotate under the drive of an external motor and drive the clamping claw (56) to rotate; The condensation water barrier module (59) comprises a support frame (510) arranged parallel to the cooling base (52), and a rotating shaft (512) connected to a first cover plate (511) is provided on the top of the support frame (510); the first cover plate (511) is fixedly connected to a flexible cover plate (514) through an elastic member (513), so that the flexible cover plate (514) can seal and cover the PCR plate (55), and the support frame (510) is fixedly connected to a driving unit (515) for driving the rotating shaft (512) to rotate.
2. The reagent cooling protection device according to claim 1, characterized in that: The cooling module (51) further comprises a water tank (516) and a heat dissipation water drain (517) connected to a liquid outlet of the water tank (516); the liquid outlet of the heat dissipation water drain (517) is connected to the cooling water drain (53); and the liquid outlet of the cooling water drain (53) is connected to the water tank (516).
3. The reagent cooling protection device according to claim 2, characterized in that: The heat dissipation water row (517) is provided with a heat dissipation pipeline.
4. The reagent cooling protection device according to claim 1 or 2, characterized in that: The cooling water drain (53) is provided with a condensed water drain hole (518) penetrating in a vertical direction.
5. The reagent cooling protection device according to claim 1 or 2, characterized in that: The cooling module (51) further comprises a semiconductor cooling plate (519) which is arranged on the top of the cooling water row (53).
6. The reagent cooling protection device according to claim 5, characterized in that: Temperature sensors are respectively provided on both sides of the semiconductor cooling plate (519).
7. The reagent cooling protection device according to claim 1, characterized in that: The driving unit (515) is a motor; The output shaft of the motor extends to one side of the outside of the support frame (510) and is connected to a first synchronous wheel (520); the first synchronous wheel (520) is connected to a second synchronous wheel (521) at one end of the rotating shaft (512) via a synchronous belt (522).
8. The reagent cooling protection device according to claim 1, characterized in that: The elastic member (513) is a telescopic pin.