Polypeptide solid-phase synthesis device

Abstract

The utility model discloses a polypeptide solid-phase synthesis device which comprises a base plate, a cylinder, a filter plate, a clamping assembly, a cleaning assembly, a liquid outlet pipe and a gas source connecting pipe, the cylinder is arranged on one side of the base plate, and the axial direction of the cylinder is the same as the thickness direction of the base plate; the filter plate is arranged in the cylinder body; the clamping assembly is arranged in the cylinder body and is connected with the chassis and the filter plate; the cleaning assembly is connected to the side, away from the chassis, of the clamping assembly and responds to the driving force to clean the inner wall of the barrel. The liquid outlet pipe is arranged on the side, away from the barrel, of the base plate and communicates with the barrel. The air source connecting pipe is arranged on the side, away from the barrel, of the base plate and communicates with the barrel. The air source connecting pipe communicates with an external air source. When cleaning is needed, only the inner wall of the barrel needs to be cleaned through the cleaning assembly, cleaning can be completed without detaching the barrel, a stirring shaft, stirring blades and the like are not used in the stirring process, and cleaning operation is more convenient.

Description

Technical Field

[0001] The present application relates to the technical field of polypeptide synthesis, and in particular to a polypeptide solid phase synthesis device. Background Art

[0002] Polypeptide is a biologically active substance related to various cell functions in the body. It is a general term for biologically active substances related to various cell functions in the body. It is often used in functional analysis, antibody research, and drug development. A peptide synthesizer refers to an instrument used to synthesize polypeptides.

[0003] The utility model patent with the publication number CN214193105U discloses a synthesis device for solid phase synthesis of polypeptides, including a chassis, a fixed plate symmetrically arranged on the inner side wall of the chassis, a slide fixedly installed on one side of the fixed plate, two cylinders fixedly installed on the inner top wall of the chassis, a long plate fixedly installed on the output end of the cylinder, a motor fixedly installed on the top of the long plate, a lid fixedly installed on the bottom of the long plate, a synthesis tank arranged on the inner bottom wall of the chassis, a stirring shaft fixedly connected to the output end of the motor, one end of the stirring shaft passes through the synthesis tank and extends to the inside of the synthesis tank, stirring blades are symmetrically arranged on both sides of the stirring shaft, a feed pipe is symmetrically arranged inside the lid, one end of the feed pipe passes through and extends to the outside of the lid, and one end of the feed pipe passes through the synthesis tank and extends to the inside of the synthesis tank. When in use, the lid and the synthesis tank are separated by the cylinder, so that the synthesis tank can be removed for cleaning or replacement.

[0004] However, the above-mentioned solid phase peptide synthesis device is only convenient for cleaning or replacing the synthesis tank, but not for cleaning the stirring shaft and stirring blades. When lifting the lid to separate the synthesis tank, the stirring shaft needs to be extended from the synthesis tank, which is inconvenient to use. Utility Model Content

[0005] The main purpose of the present application is to provide a polypeptide solid phase synthesis device, aiming to solve the problem that the existing solid phase synthesis polypeptide synthesis device is inconvenient to clean the stirring shaft and stirring blades and is inconvenient to use.

[0006] To achieve the above-mentioned purpose, the present application provides a polypeptide solid-phase synthesis device, which comprises a chassis, a cylinder, a filter plate, a clamping assembly, a cleaning assembly, a liquid outlet pipe and an air source connecting pipe, wherein the cylinder is arranged on one side of the chassis and its axial direction is the same as the thickness direction of the chassis; the filter plate is arranged in the cylinder; the clamping assembly is arranged in the cylinder and connects the chassis and the filter plate; the cleaning assembly is connected to the side of the clamping assembly away from the chassis and cleans the inner wall of the cylinder in response to a driving force; the liquid outlet pipe is arranged on the side of the chassis away from the cylinder and is connected to the cylinder; the air source connecting pipe is arranged on the side of the chassis away from the cylinder and is connected to the cylinder, and the air source connecting pipe is connected to an external air source.

[0007] Optionally, the polypeptide solid phase synthesis device also includes a connecting platform, which is fixed to the side of the chassis facing the cylinder and has a thread on the outer periphery, and an internal thread is provided at one end of the cylinder close to the chassis, and the cylinder is threadedly connected to the connecting platform; wherein the clamping assembly is provided on the side of the connecting platform away from the chassis, and the connecting platform is provided with a through hole extending along the thickness direction of the chassis for the liquid outlet pipe or the gas source pipe to connect to the cylinder.

[0008] Optionally, the clamping assembly includes two support rods, two sliders, a movable ring and a fixed ring, the two support rods extend along the thickness direction of the chassis and are fixed to the connecting platform, and the two support rods are oppositely arranged and close to each other and are provided with a slide groove extending along the thickness direction of the chassis; the two sliders slide in a one-to-one correspondence with the two slide grooves; the movable ring is fixed between the two sliders and its axial direction is the same as the axial direction of the cylinder; the fixed ring is fixed to one end of the two support rods away from the connecting platform and its axial direction is the same as the axial direction of the cylinder; wherein, the filter plate is clamped between the movable ring and the fixed ring, and the cleaning assembly is arranged on the side of the fixed ring away from the movable ring.

[0009] Optionally, the outer circumference of the fixing ring fits with the inner circumference of the cylinder, and the filter plate covers the inner circumference of the fixing ring.

[0010] Optionally, the clamping assembly also includes a threaded rod, which is arranged in one of the slide grooves. The axial direction of the threaded rod is the same as the thickness direction of the chassis and one end extends out of the side of the fixed ring away from the movable ring. The threaded rod has the freedom to rotate around its own axial direction; wherein the threaded rod passes through the corresponding slider and is threadedly connected to the corresponding slider.

[0011] Optionally, the cleaning assembly includes a first spray rod, a limiting rod, a sealing cover, a rotating tube and a second spray rod, wherein the first spray rod is arranged on a side of the fixed ring away from the movable ring and extends along the axial direction of the cylinder, and the first spray rod is provided with a plurality of nozzles facing the inner wall of the cylinder; the limiting rod is fixed to a side of the fixed ring away from the movable ring and extends along the axial direction of the cylinder; the sealing cover is fixed to an end of the limiting rod away from the fixed ring and is located in the cylinder, and a feed port is provided on the sealing cover; the axial direction of the rotating tube is the same as the axial direction of the cylinder and passes through the sealing cover, and the end of the rotating tube away from the fixed ring is connected to an external water source; the second spray rod connects the rotating tube and the first spray rod and is located in the cylinder, and a plurality of nozzles facing the sealing cover are provided on the second spray rod.

[0012] Optionally, the rotating tube is rotatably connected to the sealing cover and has the freedom to rotate around its own axial direction; the cleaning assembly also includes a rotating ring and a driving part, the rotating ring is rotatably connected to the side of the fixed ring away from the movable ring and the axial direction is the same as the axial direction of the cylinder, and the rotating ring has the freedom to rotate around its own axial direction; the driving part is arranged at the end of the sealing cover away from the chassis and connected to the rotating tube, and the driving part provides rotational driving force for the rotating tube; wherein the limit rod is located on the periphery of the rotating ring.

[0013] Optionally, the minimum distance a between the limiting rod and the central axis of the cylinder, the maximum distance b between the first spraying rod and the central axis of the cylinder, and a>b.

[0014] Optionally, the driving unit includes a motor, a driving gear and a driven gear, the motor is fixed to the side of the cover away from the fixing ring and the axial direction of the output shaft is the same as the axial direction of the cylinder; the driving gear is sleeved on the outer periphery of the output shaft of the motor; the driven gear is sleeved on the outer periphery of the rotating tube and meshes with the driving gear.

[0015] Optionally, the polypeptide solid phase synthesis device further comprises a gripping ring, which is sleeved on the outer circumference of the cylinder.

[0016] A polypeptide solid phase synthesis device proposed in an embodiment of the present application clamps a filter plate through a clamping component, places a resin on the filter plate, introduces the polypeptide solid phase synthesis solution from one end of a cylinder away from a bottom plate, connects a nitrogen gas source to the outside of a gas source pipe, and introduces nitrogen into the cylinder to continuously stir the solution in the cylinder to accelerate polypeptide synthesis, and excess nitrogen is discharged from one end of the cylinder away from the bottom plate, and excess solution is discharged from a liquid outlet pipe; when cleaning is required, only the inner wall of the cylinder needs to be cleaned through the cleaning component, and the cleaning can be completed without disassembling the cylinder, and no stirring shaft and stirring blades are used during the stirring process, so the cleaning operation is more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A schematic diagram of the overall structure of a polypeptide solid phase synthesis device provided in an embodiment of the present application;

[0018] Figure 2 for Figure 1 A structural schematic diagram of another perspective of the embodiment;

[0019] Figure 3 for Figure 1 A magnified view of the structure at center;

[0020] Figure 4 for Figure 1 A schematic diagram of the structure of the embodiment after removing the cylinder and the grip ring;

[0021] Figure 5This is a schematic diagram of the disassembled structure of the sliding part of an embodiment of the present application.

[0022] In the figure: 1, chassis; 11, support leg; 2, cylinder; 21, grip ring; 3, filter plate; 4, liquid outlet pipe; 5, air source pipe; 6, connecting platform; 71, support rod; 711, slide groove; 72, slider; 73, moving ring; 74, fixed ring; 75, threaded rod; 81, first spray rod; 82, limit rod; 83, sealing cover; 831, feed port; 84, rotating tube; 85, second spray rod; 86, rotating ring; 91, motor; 92, driving gear; 93, driven gear.

[0023] The realization of the purpose, functional features and advantages of this application will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0026] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0027] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the utility model.

[0028] refer to Figure 1 to Figure 5 It should be understood that Figure 5 The connections between the components should be like Figure 4 As compact as in the example, this is just an example. Figure 5 Some components are displayed separately for easy understanding. The embodiment of the present application provides a polypeptide solid phase synthesis device, which includes a chassis 1, a barrel 2, a filter plate 3, a clamping assembly, a cleaning assembly, a liquid outlet pipe 4 and an air source connecting pipe 5. The barrel 2 is arranged on one side of the chassis 1 and the axial direction is the same as the thickness direction of the barrel 1; the filter plate 3 is arranged in the barrel 2; the clamping assembly is arranged in the barrel 2 and connects the chassis 1 and the filter plate 3; the cleaning assembly is connected to the side of the clamping assembly away from the chassis 1 and cleans the inner wall of the barrel 2 in response to the driving force; the liquid outlet pipe 4 is arranged on the side of the chassis 1 away from the barrel 2 and is connected to the barrel 2; the air source connecting pipe 5 is arranged on the side of the chassis 1 away from the barrel 2 and is connected to the barrel 2, and the air source connecting pipe 5 is connected to an external air source.

[0029] A polypeptide solid phase synthesis device proposed in an embodiment of the present application clamps a filter plate 3 by a clamping assembly, a resin is placed on the filter plate 3, a polypeptide solid phase synthesis solution is introduced from an end of a cylinder 2 away from a bottom plate 1, a gas source pipe 5 is externally connected to a nitrogen gas source, nitrogen is introduced into the cylinder 2 to continuously stir the solution in the cylinder 2 to accelerate polypeptide synthesis, excess nitrogen is discharged from an end of the cylinder 2 away from the bottom plate 1, and excess solution is discharged from a liquid outlet pipe 4; when cleaning is required, only the inner wall of the cylinder 2 needs to be cleaned by the cleaning assembly, and cleaning can be completed without disassembling the cylinder 2, and no stirring shaft and stirring blades are used during the stirring process, so the cleaning operation is more convenient.

[0030] Specifically, the gas source pipe 5 is connected to the external gas source, which can provide stable pressure, help control the reaction environment, ensure the uniformity and repeatability of the reaction, thereby improving the synthesis efficiency and product quality; the cleaning component can effectively clean the inner wall of the cylinder 2, reduce the interference of residues, and ensure the purity of each reaction.

[0031] It should be noted that both the liquid outlet pipe 4 and the gas source connecting pipe 5 are provided with a one-way valve to control the flow between the liquid outlet pipe 4 and the gas source connecting pipe 5 .

[0032] A plurality of supporting legs 11 are arranged on the side of the chassis 1 facing away from the cylinder 2 to support the polypeptide solid phase synthesis device.

[0033] refer to Figure 2 and Figure 4 In an exemplary embodiment, the polypeptide solid phase synthesis device may further include a connecting platform 6, which is fixed to the side of the chassis 1 facing the barrel 2 and is provided with a thread on the outer periphery, and an internal thread is provided at one end of the barrel 2 close to the chassis 1, and the barrel 2 is threadedly connected to the connecting platform 6; wherein the clamping assembly is provided on the side of the connecting platform 6 away from the chassis 1, and a through hole extending along the thickness direction of the chassis 1 is provided on the connecting platform 6 for the liquid outlet pipe 4 or the gas source pipe 5 to connect to the barrel 2.

[0034] Specifically, the cylinder 2 is threadedly connected to the connecting platform 6, so that the installation and removal of the cylinder 2 becomes easier and faster, and it is convenient for the maintenance, cleaning and replacement of parts of the equipment. The threaded connection provides mechanical stability to ensure that the cylinder 2 will not loosen easily when subjected to pressure or vibration. At the same time, the threaded fit can achieve a good seal to prevent leakage of gas or liquid during the reaction process.

[0035] Among them, Figure 4 As shown, the through holes on the connecting platform 6 can ensure that the liquid outlet pipe 4 and the gas source pipe 5 can be connected to the cylinder 2. It should be understood that the chassis 1 is also provided with through holes for the liquid outlet pipe 4 and the gas source pipe 5 to be connected to the cylinder 2.

[0036] refer to Figure 1 In an exemplary embodiment, the polypeptide solid phase synthesis device may further include a gripping ring 21, which is sleeved on the outer circumference of the cylinder 2, so that it is more convenient to rotate the cylinder 2 by rotating the gripping ring 21. In other words, it is more convenient to install or remove the cylinder 2, and it is also convenient to transfer the cylinder 2 through the gripping ring 21.

[0037] refer to Figure 5In an exemplary embodiment, the clamping assembly may include two support rods 71, two sliders 72, a movable ring 73 and a fixed ring 74. The two support rods 71 ​​extend along the thickness direction of the chassis 1 and are fixed to the connecting platform 6. The two support rods 71 ​​are arranged opposite to each other and are provided with a slide groove 711 extending along the thickness direction of the chassis 1 on one side close to each other; the two sliders 72 slide in a one-to-one correspondence with the two slide grooves 711; the movable ring 73 is fixed between the two sliders 72 and its axial direction is the same as that of the cylinder 2; the fixed ring 74 is fixed to one end of the two support rods 71 ​​away from the connecting platform 6 and its axial direction is the same as that of the cylinder 2; wherein the filter plate 3 is clamped between the movable ring 73 and the fixed ring 74, and the cleaning assembly is arranged on the side of the fixed ring 74 away from the movable ring 73.

[0038] Specifically, the slider 72 and the slide groove 711 can be interference fit, so that the moving ring 73 can be stopped at any time when moving along the axial direction of the cylinder 2, and the filter plate 3 can be clamped or loosened by controlling the distance between the moving ring 73 and the fixed ring 74, which makes it easy to install or remove the filter plate 3.

[0039] There are two support rods 71 ​​which are arranged opposite to each other. When the moving ring 73 slides through the slider 72, the two sliders 72 respectively support the two opposite sides of the outer circumference of the moving ring 73, so that the moving process of the moving ring 73 is more stable.

[0040] Furthermore, the outer circumference of the fixing ring 74 is in contact with the inner circumference of the cylinder 2 , and the filter plate 3 covers the inner circumference of the fixing ring 74 , thereby preventing the resin from moving to the other side of the filter plate 3 and causing the resin to be discharged from the liquid outlet pipe 4 .

[0041] In an exemplary embodiment, the clamping assembly may also include a threaded rod 75, which is disposed in a slide groove 711. The axial direction of the threaded rod 75 is consistent with the thickness direction of the chassis 1 and one end extends out of the fixed ring 74 away from the side of the movable ring 73. The threaded rod 75 has the freedom to rotate around its own axial direction; wherein the threaded rod 75 passes through the corresponding slider 72 and is threadedly connected to the corresponding slider 72.

[0042] Specifically, by rotating the threaded rod 75 to drive the corresponding slider 72 to move in the slide groove 711, the position of the slider 72 in the slide groove 711 can be effectively controlled, that is, the distance between the movable ring 73 and the fixed ring 74 can be controlled to clamp or loosen the filter plate 3; at the same time, when the solution is passed into the cylinder 2, the solution will apply a force to the filter plate 3 to approach the chassis 1 through the filter plate 3. When the threaded rod 75 is used to control the position of the movable ring 73, the filter plate 3 cannot drive the movable ring 73 away from the fixed ring 74, thereby ensuring the stability of the clamping assembly in clamping the filter plate 3.

[0043] refer to Figure 4 and Figure 5In an exemplary embodiment, the cleaning assembly may include a first spray rod 81, a limiting rod 82, a sealing cover 83, a rotating tube 84 and a second spray rod 85, wherein the first spray rod 81 is arranged on a side of the fixed ring 74 away from the movable ring 73 and extends along the axial direction of the cylinder 2, and the first spray rod 81 is provided with a plurality of nozzles facing the inner wall of the cylinder 2; the limiting rod 82 is fixed to a side of the fixed ring 74 away from the movable ring 73 and extends along the axial direction of the cylinder 2; the sealing cover 83 is fixed to an end of the limiting rod 82 away from the fixed ring 74 and is located in the cylinder 2, and a feed port 831 is provided on the sealing cover 83; the axial direction of the rotating tube 84 is the same as that of the cylinder 2 and passes through the sealing cover 83, and the end of the rotating tube 84 away from the fixed ring 74 is connected to an external water source; the second spray rod 85 connects the rotating tube 84 and the first spray rod 81 and is located in the cylinder 2, and a plurality of nozzles facing the sealing cover 83 are provided on the second spray rod 85.

[0044] Specifically, the rotating tube 84 is connected to an external water source. After the external water source enters the rotating tube 84, it flows into the second spray rod 85 and the first spray rod 81 in turn, and then is sprayed out from the nozzles set on the second spray rod 85 and the first spray rod 81 to clean the cylinder 2 and the cover 83. The waste liquid after cleaning can be discharged from the liquid outlet pipe 4, and the cleaning is completed in this way.

[0045] Furthermore, the sealing cap 83 seals one end of the cylinder 2, and the sealing cap 83 is located on the inner circumference of the cylinder 2, so that it will not affect the disassembly of the cylinder 2 and can also play a sealing role to prevent impurities from entering the cylinder 2 and affecting the synthesis effect.

[0046] It should be noted that the cover 83 is provided with a feed port 831, through which the solution can be added into the cylinder 2. When nitrogen is stirred, the excess gas in the cylinder 2 can also be discharged from the feed port 831.

[0047] refer to Figure 4 and Figure 5 In an exemplary embodiment, the rotating tube 84 is rotatably connected to the sealing cover 83 and has the freedom to rotate around its own axial direction; the cleaning assembly may also include a rotating ring 86 and a driving unit, the rotating ring 86 is rotatably connected to the side of the fixed ring 74 away from the movable ring 73 and the axial direction is the same as the axial direction of the cylinder 2, and the rotating ring 86 has the freedom to rotate around its own axial direction; the driving unit is arranged at the end of the sealing cover 83 away from the chassis 1 and is connected to the rotating tube 84, and the driving unit provides a rotational driving force for the rotating tube 84; wherein the limiting rod 82 is located on the periphery of the rotating ring 86.

[0048] Specifically, the driving unit drives the rotating tube 84 to rotate around its own axis, the rotating tube 84 drives the second spray rod 85 and the first spray rod 81 to rotate around the axis of the rotating tube 84, and the first spray rod 81 drives the rotating ring 86 to rotate around the axis of the rotating tube 84. During the rotation process, the first spray rod 81 and the second spray rod 85 continuously spray water to clean the cover 83 and the cylinder 2, and the cleaning effect is better.

[0049] Furthermore, the rotating tube 84 may not be connected to the external water source during the rotation process, so that the first spray rod 81 and the second spray rod 85 are driven to rotate by the driving unit, so that the solution in the cylinder 2 can be stirred, and the polypeptide synthesis effect is better.

[0050] The rotating ring 86 supports one end of the first spray rod 81 , and the second spray rod 85 and the rotating tube 84 support the other end of the first spray rod 81 , so that the rotation process of the first spray rod 81 is more stable.

[0051] refer to Figure 4 In an exemplary embodiment, the minimum distance a between the limiting rod 82 and the central axis of the barrel 2, the maximum distance b between the first spraying rod 81 and the central axis of the barrel 2, and a>b.

[0052] Specifically, a is the minimum vertical distance between all points on the limit rod 82 and the central axis of the cylinder 2, b is the maximum vertical distance between the points on the first spray rod 81 and the central axis of the cylinder 2, and the rotation path of the first spray rod 81 is a first circle. When a is greater than b, the limit rod 82 is located outside the first circle. In other words, the limit rod 82 is not on the moving path of the first spray rod 81, ensuring that the rotation of the first spray rod 81 will not be affected by the limit rod 82.

[0053] refer to Figure 3 In an exemplary embodiment, the driving unit may include a motor 91, a driving gear 92 and a driven gear 93. The motor 91 is fixed to the side of the cover 83 away from the fixing ring 74 and the axial direction of the output shaft is the same as the axial direction of the cylinder 2; the driving gear 92 is sleeved on the outer periphery of the output shaft of the motor 91; the driven gear 93 is sleeved on the outer periphery of the rotating tube 84 and meshes with the driving gear 92.

[0054] Specifically, the motor 91 can be connected to an external power source, or a battery can be set on the primary side of the cover 83 away from the chassis 1 to provide power to the motor 91; the motor 91 drives the driving gear 92 to rotate around the output shaft of the motor 91, and the driven gear 93 is sleeved on the outer periphery of the rotating tube 84 and meshes with the driving gear 92. When the driving gear 92 rotates, it will drive the driven gear 93 and the rotating tube 84 to rotate.

[0055] The above are only preferred embodiments of the present application, and are not intended to limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A polypeptide solid phase synthesis device, characterized in that: include: Chassis (1); A cylinder (2) is arranged on one side of the chassis (1) and has an axial direction which is the same as the thickness direction of the chassis (1); A filter plate (3) is arranged in the cylinder (2); A clamping assembly, disposed in the cylinder (2) and connecting the base plate (1) and the filter plate (3); A cleaning component connected to a side of the clamping component facing away from the chassis (1) and cleaning the inner wall of the cylinder (2) in response to a driving force; A liquid outlet pipe (4) is arranged on a side of the bottom plate (1) away from the cylinder (2) and is in communication with the cylinder (2); An air source connecting pipe (5) is arranged on a side of the chassis (1) away from the cylinder (2) and is in communication with the cylinder (2); the air source connecting pipe (5) is in communication with an external air source.

2. The polypeptide solid phase synthesis device according to claim 1, characterized in that: The polypeptide solid phase synthesis device also includes: A connecting platform (6) is fixed to a side of the base plate (1) facing the cylinder (2) and is provided with threads on its outer circumference. An end of the cylinder (2) close to the base plate (1) is provided with internal threads. The cylinder (2) is threadedly connected to the connecting platform (6); The clamping assembly is arranged on a side of the connecting platform (6) away from the chassis (1), and the connecting platform (6) is provided with a through hole extending in the thickness direction of the chassis (1) so as to allow the liquid outlet pipe (4) or the gas source pipe (5) to be connected to the cylinder (2).

3. The polypeptide solid phase synthesis device according to claim 2, characterized in that: The clamping assembly comprises: Two support rods (71) are both extended in the thickness direction of the chassis (1) and are fixed to the connecting platform (6); the two support rods (71) are arranged opposite to each other and are both provided with a slide groove (711) extending in the thickness direction of the chassis (1) on one side close to each other; Two sliding blocks (72) are slidably matched with the two sliding grooves (711) in a one-to-one correspondence; A movable ring (73) is fixed between the two sliders (72) and has an axial direction which is the same as that of the cylinder (2); A fixing ring (74), fixed to one end of the two support rods (71) away from the connecting platform (6) and having an axial direction which is the same as that of the cylinder (2); The filter plate (3) is clamped between the movable ring (73) and the fixed ring (74), and the cleaning component is arranged on a side of the fixed ring (74) facing away from the movable ring (73).

4. The polypeptide solid phase synthesis device according to claim 3, characterized in that: The outer periphery of the fixing ring (74) is in contact with the inner periphery of the cylinder (2), and the filter plate (3) covers the inner periphery of the fixing ring (74).

5. The polypeptide solid phase synthesis device according to claim 3, characterized in that: The clamping assembly also includes: A threaded rod (75) is arranged in one of the slide grooves (711), the axial direction of the threaded rod (75) is in the same direction as the thickness direction of the chassis (1), one end of the threaded rod (75) extends out of the fixed ring (74) away from the movable ring (73), and the threaded rod (75) has the freedom to rotate around its own axial direction; Wherein, the threaded rod (75) passes through the corresponding slider (72) and is threadedly connected to the corresponding slider (72).

6. The polypeptide solid phase synthesis device according to claim 3, characterized in that: The cleaning assembly comprises: a first spray rod (81), which is arranged on a side of the fixed ring (74) away from the movable ring (73) and extends along the axial direction of the cylinder (2); the first spray rod (81) is provided with a plurality of nozzles facing the inner wall of the cylinder (2); A limiting rod (82) is fixed to a side of the fixed ring (74) away from the movable ring (73) and extends along the axial direction of the cylinder (2); A sealing cover (83) is fixed to an end of the limiting rod (82) away from the fixing ring (74) and is located inside the cylinder (2), and a feed port (831) is provided on the sealing cover (83); A rotating tube (84) having an axial direction which is the same as that of the cylinder (2) and passes through the sealing cover (83); an end of the rotating tube (84) which is away from the fixing ring (74) is in communication with an external water source; The second spray rod (85) is connected to the rotating tube (84) and the first spray rod (81) and is located in the cylinder (2). The second spray rod (85) is provided with a plurality of nozzles facing the sealing cover (83).

7. The polypeptide solid phase synthesis device according to claim 6, characterized in that: The rotating tube (84) is rotatably connected to the sealing cover (83) and has the freedom to rotate around its own axis; the cleaning assembly also includes: a rotating ring (86) rotatably connected to a side of the fixed ring (74) away from the movable ring (73) and having an axial direction which is the same as the axial direction of the cylinder (2); the rotating ring (86) has a degree of freedom to rotate around its own axial direction; a driving unit, which is arranged at one end of the cover (83) away from the chassis (1) and is connected to the rotating tube (84), and the driving unit provides a rotational driving force for the rotating tube (84); Wherein, the limiting rod (82) is located on the periphery of the rotating ring (86).

8. The polypeptide solid phase synthesis device according to claim 7, characterized in that: The minimum distance a between the limiting rod (82) and the central axis of the cylinder (2), the maximum distance b between the first spraying rod (81) and the central axis of the cylinder (2), and a>b.

9. The polypeptide solid phase synthesis device according to claim 7, characterized in that: The driving unit comprises: A motor (91) is fixed to a side of the sealing cover (83) away from the fixing ring (74) and the axial direction of the output shaft is the same as the axial direction of the cylinder (2); A driving gear (92) sleeved on the outer circumference of the output shaft of the motor (91); The driven gear (93) is sleeved on the outer periphery of the rotating tube (84) and meshes with the driving gear (92).

10. The polypeptide solid phase synthesis device according to claim 2, characterized in that: The polypeptide solid phase synthesis device also includes: The grip ring (21) is sleeved on the outer circumference of the cylinder (2).

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