Immunoaffinity operation instrument

Abstract

The utility model provides an immunoaffinity operation instrument. The immunoaffinity operation instrument comprises a leaching and eluting assembly, a sample disc assembly, a collecting disc assembly, a cap removing mechanism and a waste cap collecting box, the sample disc assembly comprises a first fixing plate, a second fixing plate, a third fixing plate and a first water receiving disc are sequentially arranged below the first fixing plate in the vertical direction, a sleeve is arranged between the first fixing plate and the second fixing plate, a sample tube assembly is arranged in the sleeve, and a first driving mechanism is installed at the bottom of the sample disc assembly; the collecting disc assembly comprises a collecting disc body, a second driving device is installed below the collecting disc body, and a sleeve row is arranged on the collecting disc body. A contact type detection switch is arranged between the collecting disc assembly and the sample disc assembly, the contact type detection switch is electrically connected with the first driving mechanism, and the device has the advantages of being simple in structure, convenient to use and stable in performance.

Description

Technical Field

[0001] The utility model relates to the technical field of biological instruments, in particular to an immunoaffinity operation instrument. Background Art

[0002] The full name of the immunoaffinity column is immunoaffinity chromatography column, which is often abbreviated as affinity column, immunoaffinity column, affinity chromatography column, etc.; the principle of the immunoaffinity column is to covalently bind an antibody to an inert microbead, make an affinity adsorbent and pack it into a column, and then pass an antigen solution through the immunoaffinity column, while non-target compounds flow down the column, and finally the antigen is eluted with an elution buffer to obtain a purified antigen. Using the high specificity of the immunoaffinity column, pure sample extracts can be obtained by immunoaffinity column purification.

[0003] Taking the extraction of aflatoxin from corn as an example, the extraction method includes the following steps: weighing the crushed sample into a centrifuge tube, adding methanol-water (7:3) for extraction, and after vortex mixing, homogenizing, centrifuging, and filtering, obtaining a crude sample extract; after combining the immunoaffinity column with a sample loading tube, placing it on an operation rack, transferring the filtrate, and then respectively treating it with a washing solution and an elution solution to obtain purified aflatoxin.

[0004] Patent Application Publication No. CN202223181981.3 discloses an immunoaffinity operation instrument, which can realize automatic uncapping, sample enrichment by passing, washing, elution, and collection of the immunoaffinity column through the cooperation of a sample rotation device, an uncapping device, a washing device, an elution device, and a collection rotation device, and can batch extract samples, greatly improving work efficiency and reducing labor intensity.

[0005] Although the above-mentioned prior art can perform automatic uncapping, sample enrichment by passing, washing, elution, and collection of the immunoaffinity column, and can batch extract samples, greatly improving work efficiency and reducing labor intensity, the overall effect is poor.

[0006] When placing a sample tube in the existing immunoaffinity operation instrument, it is easy to get stuck if the direction is not perpendicular, the operation is not smooth, and it is necessary to place the sample tubes in order without gaps in the middle, resulting in reduced operation flexibility; there are also problems such as the collection tray being unable to hold multiple collection bottles at the same time. Summary of the Utility Model

[0007] The technical problem to be solved by the utility model is that when placing a sample tube in the existing immunoaffinity operation instrument, it is easy to get stuck if the direction is not perpendicular, the operation is not smooth, and it is necessary to place the sample tubes in order without gaps in the middle, resulting in reduced operation flexibility; the collection tray cannot hold multiple collection bottles at the same time.

[0008] To solve the above technical problems, the present utility model provides an immunoaffinity operation instrument, which includes a washing and elution assembly, a sample tray assembly, a collection tray assembly, a cap removal mechanism and a waste cap collection box. The washing and elution assembly is located between the sample tray assembly and the collection tray assembly for performing washing and elution on the immunoaffinity column. The cap removal mechanism is located below the sample tray assembly for removing the cap of the immunoaffinity column. The sample tray assembly includes a first fixing plate. Vertically below the first fixing plate, there are successively a second fixing plate, a third fixing plate and a first water receiving tray. Between the first fixing plate, the second fixing plate and the third fixing plate, there are isolation columns. Between the first fixing plate and the second fixing plate, there is a sleeve. Inside the sleeve, there is a sample tube assembly. A first driving mechanism is installed at the bottom of the sample tray assembly. The collection tray assembly includes a collection tray body. A second driving device is installed below the collection tray body. The collection tray body is provided with a sleeve row. A contact detection switch is arranged between the collection tray assembly and the sample tray assembly for detecting whether there is an empty space on the sample tray assembly. The contact detection switch is electrically connected to the first driving mechanism.

[0009] Further, the sample tube assembly includes a loading tube. The loading tube is placed inside the sleeve. The bottom of the loading tube is connected to an immunoaffinity column. The bottom end of the immunoaffinity column is provided with a cap. The immunoaffinity column passes through the second fixing plate and the cap is located inside the first water receiving tray.

[0010] Further, the sleeve row is arc-shaped and fits the upper surface of the collection tray body. The sleeve row is provided with limiting holes. The limiting holes correspond to the second placement holes on the collection tray body.

[0011] Further, a step is provided in the middle of the second placement hole.

[0012] Further, a second water receiving tray is provided below the collection tray body. The second water receiving tray corresponds to the bottom of the second placement hole.

[0013] Further, the first driving mechanism includes a support column. The top of the support column passes through the first water receiving tray and the third fixing plate. A motor is arranged inside the support column. A speed reducer is installed on the rotating shaft of the motor. The speed reducer is connected to the second fixing plate through a flange coupling.

[0014] Further, a plurality of first placement holes are provided on each of the first fixing plate, the second fixing plate and the third fixing plate.

[0015] Advantages of the present utility model:

[0016] 1. The sample tray assembly is mainly vertically arranged with a first fixing plate, a second fixing plate and a third fixing plate. Multiple sample tube sleeves are clamped between the first fixing plate and the second fixing plate. When the sample tube assembly is placed on the sample tray, it can play a good role in guiding and positioning, improving the operation efficiency.

[0017] 2. The sample tray assembly and the collection tray assembly are installed with contact detection switches at corresponding positions. During operation, the sample tube assembly can be placed arbitrarily. When the sample tray assembly and the collection tray assembly rotate and encounter an empty position, it can be skipped directly.

[0018] 3. A step is provided in the middle of the second placement hole of the collection tray assembly. In addition, by placing a sleeve row on the collection tray assembly, more specifications of collection bottles can be accommodated. Description of the Drawings

[0019] Figure 1 It is a perspective view of an immunoaffinity operation instrument.

[0020] Figure 2 It is a schematic structural diagram of the sample tray assembly in an immunoaffinity operation instrument.

[0021] Figure 3 It is a schematic partial structural diagram of the sample tray assembly in an immunoaffinity operation instrument.

[0022] Figure 4 It is an enlarged view of part A in the perspective view of an immunoaffinity operation instrument.

[0023] Figure 5 It is a perspective view of the collection tray assembly in an immunoaffinity operation instrument.

[0024] Figure 6 It is a schematic partial structural diagram of the collection tray assembly in an immunoaffinity operation instrument.

[0025] As shown in the figure:

[0026] 1. Washing and elution assembly; 2. Sample tray assembly; 201. First fixing plate; 202. Second fixing plate; 203. Third fixing plate; 204. Isolation column; 205. Sample tube assembly; 2051. Sampling tube; 2052. Immunoaffinity column; 2053. Cap; 206. Sleeve; 207. First placement hole; 3. Collection tray assembly; 301. Collection tray body; 302. Sleeve row; 303. Step; 304. Second placement hole; 4. Cap removal mechanism; 5. Waste cap collection box; 6. First drive mechanism; 601. Support column; 602. Motor; 603. Reducer; 604. Flange coupling; 8. Contact detection switch; 9. First water receiving tray; 10. Second water receiving tray. Detailed Embodiments

[0027] The following further illustrates the detailed embodiments of the present invention with reference to the drawings. The same components are denoted by the same reference numerals.

[0028] It should be noted that the terms "front", "rear", "left", "right", "up" and "down" used in the following description refer to the directions in the accompanying drawings, and the terms "inner" and "outer" refer to the directions towards or away from the geometric center of a specific component respectively.

[0029] To make the content of the present utility model easier to be clearly understood, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.

[0030] Combined with the attached Figures 1-6 As shown, an immunoaffinity operation instrument provided by the present utility model includes a washing and elution assembly 1, a sample tray assembly 2, a collection tray assembly 3, a cap removal mechanism 4 and a waste cap collection box 5. The washing and elution assembly 1 is located between the sample tray assembly 2 and the collection tray assembly 3 and is used for washing and eluting the immunoaffinity column 2052. The cap removal mechanism 4 is located below the sample tray assembly 2 and is used for removing the cap of the immunoaffinity column 2052.

[0031] The sample tray assembly 2 includes a first fixing plate 201. A second fixing plate 202, a third fixing plate 203 and a first water receiving tray 9 are successively arranged vertically below the first fixing plate 201. Isolation columns 204 are arranged between the first fixing plate 201, the second fixing plate 202 and the third fixing plate 203. A sleeve 206 is arranged between the first fixing plate 201 and the second fixing plate 202, and a sample tube assembly 205 is arranged inside the sleeve 206.

[0032] A number of first placement holes 207 are arranged on each of the first fixing plate 201, the second fixing plate 202 and the third fixing plate 203.

[0033] The sample tray assembly 2 is mainly vertically arranged by the first fixing plate 201, the second fixing plate 202 and the third fixing plate 203. Multiple sample tube sleeves 206 are clamped between the first fixing plate 201 and the second fixing plate 202, which can play a good role in guiding and positioning when the sample tube assembly 2 is placed on the sample tray, improving the operation efficiency.

[0034] A first driving mechanism 6 is installed at the bottom of the sample tray assembly 2, and the first driving mechanism 6 is used to drive the sample tray assembly 2 to rotate.

[0035] The collection tray assembly 3 includes a collection tray body 301. A second driving device is installed below the collection tray body 301, and a sleeve row 206 is arranged on the collection tray body 301.

[0036] The specific structure of the second driving device has been disclosed in an immunoaffinity operation instrument with the application publication number CN202223181981.3.

[0037] The sleeve row 206 is arc-shaped and fits on the upper surface of the collection tray body 301. The sleeve row 206 is provided with limiting holes, and the limiting holes correspond to the second placement holes 304 of the collection tray body 301.

[0038] A step 303 is provided in the middle of the second placement hole 304.

[0039] By placing the sleeve row 206 and the step 303 in the collection tray assembly 3, more specifications of collection bottles can be accommodated.

[0040] A contact detection switch 8 is provided between the collection tray assembly 3 and the sample tray assembly 2 for detecting whether there is an empty space on the sample tray assembly 2. The contact detection switch 8 is electrically connected to the first driving mechanism 6.

[0041] When the contact detection switch 8 detects an empty space on the sample tray assembly 2, it will send a signal to the first driving mechanism 6, and then the first driving mechanism 6 drives the sample tray assembly 2 to rotate and skip the empty space for operation.

[0042] When the contact detection switch 8 touches the immunoaffinity column 2052, it will cause the built-in optoelectronic switch to block the light source, and then the optoelectronic switch sends a signal to determine whether there is an immunoaffinity column 2052 on the sample tray assembly 2.

[0043] The sample tube assembly 205 includes a sampling tube 2051. The sampling tube 2051 is placed in the sleeve 206. The bottom of the sampling tube 2051 is connected to an immunoaffinity column 2052. A cap 2053 is provided at the bottom end of the immunoaffinity column 2052. The immunoaffinity column 2052 passes through the second fixing plate 202 and the cap 2053 is located in the first water receiving tray 9.

[0044] The first water receiving tray 9 is used to receive the water in the immunoaffinity column 2052 in the collection tray assembly 3.

[0045] A second water receiving tray 10 is provided below the collection tray body 301. The second water receiving tray 10 corresponds to the bottom of the second placement hole 304. The second water receiving tray 10 is used to receive the water in the empty tubes in the collection tray body 301.

[0046] The first driving mechanism 6 includes a support column 601. The top of the support column 601 passes through the first water receiving tray 9 and the third fixing plate 203. A motor 602 is provided in the support column 601. A speed reducer 603 is installed on the rotating shaft of the motor 602. The speed reducer 603 is connected to the second fixing plate 202 through a flange coupling 604.

[0047] The motor 602 drives the collection tray assembly 3 to rotate.

[0048] Working process of the utility model: After combining the immunoaffinity column 2052 and the sample loading tube 2051, place them on the sample tray assembly 2, and then start the first driving mechanism 6 to drive the sample tray assembly 2 to rotate. When the immunoaffinity column 2052 on the sample tray assembly 2 moves to the cap removal mechanism 4, it is processed by the cap removal mechanism 4 to remove the cap 2053 of the immunoaffinity column 2052; then after elution by the elution and washing assembly 1, the eluate flows into the collection tray assembly 3 for collection.

[0049] All standard parts used in the utility model can be purchased from the market. Special-shaped parts can be customized according to the description in the specification and the drawings. The specific connection methods of each part all adopt conventional means such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts, and equipment all adopt conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be elaborated here. The content not described in detail in this specification belongs to the prior art well-known to those skilled in the art.

[0050] The above describes the utility model and its implementation manners. Such description is not restrictive. What is shown in the drawings is only one of the implementation manners of the utility model, and the actual structure is not limited thereto. Generally speaking, if those of ordinary skill in the art are inspired by it and design similar structural manners and embodiments without creative efforts without departing from the creative purpose of the utility model, they shall fall within the protection scope of the utility model.

Claims

1. An immunoaffinity operating instrument, comprising a elution assembly, a sample tray assembly, a collection tray assembly, a cap removal mechanism and a waste cap collection box, wherein the elution assembly is located between the sample tray assembly and the collection tray assembly for eluting the immunoaffinity column, and the cap removal mechanism is located below the sample tray assembly for removing the cap of the immunoaffinity column; Features: The sample tray assembly comprises a first fixed plate, a second fixed plate, a third fixed plate and a first water receiving tray are arranged in sequence in a vertical direction below the first fixed plate, a spacer column is arranged between the first fixed plate, the second fixed plate and the third fixed plate, a sleeve is arranged between the first fixed plate and the second fixed plate, a sample tube assembly is arranged in the sleeve, and a first driving mechanism is installed at the bottom of the sample tray assembly; The collection tray assembly comprises a collection tray body, a second driving device is installed below the collection tray body, and a sleeve row is provided on the collection tray body; A contact detection switch is provided between the collection tray assembly and the sample tray assembly for detecting whether there is a vacancy on the sample tray assembly; The contact detection switch is electrically connected to the first driving mechanism.

2. An immunoaffinity operating instrument according to claim 1, characterized in that: The sample tube assembly comprises a loading tube, which is placed in a sleeve, the bottom of which is connected to an immunoaffinity column, a cap is provided at the bottom of the immunoaffinity column, the immunoaffinity column passes through a second fixing plate and the cap is located in a first water receiving tray.

3. An immunoaffinity operation instrument according to claim 1, characterized in that: The sleeve row is arc-shaped and fits the upper surface of the collection tray body. The sleeve row is provided with a limiting hole, and the limiting hole corresponds to the second placement hole of the collection tray body.

4. An immunoaffinity operating instrument according to claim 3, characterized in that: A step is provided in the middle of the second placement hole.

5. An immunoaffinity operation instrument according to claim 1 or 3, characterized in that: A second water receiving tray is provided below the collecting tray body, and the second water receiving tray corresponds to the bottom of the second placement hole.

6. The immunoaffinity operating instrument according to claim 1, characterized in that: The first driving mechanism comprises a supporting column, the top of which passes through the first water receiving tray and the third fixed plate. A motor is arranged in the supporting column, a reducer is installed on the rotating shaft of the motor, and the reducer is connected to the second fixed plate through a flange coupling.

7. An immunoaffinity operating instrument according to claim 1, characterized in that: The first fixing plate, the second fixing plate and the third fixing plate are all provided with a plurality of first placement holes.

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