Enzymolysis device for protein polypeptide production
By setting up an internal heating assembly, an external heating chamber and an insulation layer in the tank of the enzymatic lysis device, the problem of uneven heating in the tank in the existing device is solved, and a more efficient enzymatic lysis process is achieved.
Patent Information
- Application Number
- CN202421898207.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-07
- Publication Date
- 2025-07-01
- Estimated Expiration
- 2034-08-07
AI Technical Summary
The existing enzymatic lysis devices cannot achieve uniform heating inside the tank during the stirring process, resulting in a longer enzymatic lysis time and a reduced working efficiency.
An enzymatic lysis device for protein polypeptide production is designed. By setting an internal heating assembly in the tank body and a heating chamber and an insulation layer outside, uniform heating and insulation inside the tank body are achieved.
It realizes uniform heating and insulation inside the tank, shortens the enzymatic lysis time, improves working efficiency, and reduces energy consumption.
Smart Images
Figure CN223047529U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of protein enzymolysis, and particularly relates to an enzymolysis device for protein polypeptide production. Background Art
[0002] The method of using biological enzymes to catalyze proteins is called the enzymatic method, and the polypeptide obtained by catalyzing proteins with enzymes is called "enzymatic polypeptide". Obtaining polypeptides by biological enzyme degradation method is of great significance. The biological enzyme degradation method is to apply the increasingly developed biological enzyme technology products to catalyze, degrade, and hydrolyze proteins to obtain polypeptides, oligopeptides, micropeptides, and small peptides with a molecular weight distribution between 1000 and 200 and composed of 2 to 4 amino acids. Obtaining polypeptides by biological enzyme degradation method is a branch of bioactive peptides. The implementation process of enzymatic polypeptides is also the third-degree deep processing of animal and plant proteins by protein engineering and bioengineering technologies. The enzymolysis of proteins is usually carried out in a dedicated enzymolysis device.
[0003] Generally, a stirring component is arranged inside the existing enzymolysis device. During stirring, only the heating outside the tank body can be realized, and the required heating temperature inside the tank body cannot be reached, which prolongs the enzymolysis time and reduces the working efficiency. Summary of the Utility Model
[0004] The purpose of the utility model is to provide an enzymolysis device for protein polypeptide production. By arranging an internal heating component inside the tank body and a heating cavity and a heat preservation layer outside, the internal heating is uniform and has heat preservation performance, thus solving the above problems existing in the prior art.
[0005] To solve the above technical problems, the utility model adopts the following scheme:
[0006] An enzymolysis device for protein polypeptide production includes a tank body with a support seat and a discharge port at the bottom. The top of the tank body is provided with a feed port. An internal heating component that rotates with a rotating shaft is arranged inside the tank body. A heating cavity and a heat preservation layer are sequentially arranged on the outer periphery of the tank body.
[0007] Preferably, the internal heating component includes a rotating cylinder and a heater located inside the rotating cylinder, and the rotating shaft penetrates through the rotating cylinder.
[0008] Preferably, a U-shaped seat is arranged below the rotating cylinder. The bottom end of the U-shaped seat is located at the bottom of the tank body. A bearing is arranged on the surface of the U-shaped seat. The bottom end of the rotating shaft is located inside the bearing, and the top end of the rotating shaft is connected to the output end of a rotating motor.
[0009] Preferably, the bottom end of the U-shaped seat is on the same vertical axis as the discharge port.
[0010] Preferably, a partition plate is arranged inside the rotating cylinder, and the partition plate is adjacent to the rotating shaft.
[0011] Preferably, through holes are respectively provided at the top and bottom of the rotating shaft, the rotating shaft passes through the through holes, and a sealing ring is provided between the through holes and the rotating shaft.
[0012] Preferably, stirring rods are respectively provided at the top of the rotating shaft, the middle part and the bottom of the rotating cylinder, and scraping plates are provided at the ends of the stirring rods adjacent to the inner wall of the tank body.
[0013] Preferably, a water injection pipe and a drain pipe are respectively provided at the top and bottom of the heating cavity, and a plurality of heating resistance wires are provided inside the heating cavity.
[0014] Preferably, a filter screen is provided inside the discharge port.
[0015] The beneficial effects of the present utility model are as follows:
[0016] In the present utility model, the output end of the rotating motor enables the rotating shaft to rotate under the bearing assembly on the surface of the U-shaped seat, driving the rotating cylinder and the heater inside it to rotate, realizing uniform heating inside the tank body, with good heat preservation performance. Moreover, the filter screen provided inside the discharge port is beneficial to reducing the impurity content after the discharge of the enzymatic hydrolysis reactant; the isolation plate provided inside the rotating cylinder avoids the direct transfer of the heat of the heater to the inside of the rotating shaft, preventing the deformation of the rotating shaft; a sealing ring is provided between the rotating shaft and the through hole, avoiding the reaction liquid inside the tank body from entering the inside of the rotating cylinder during the enzymatic hydrolysis process, preventing damage to the heater and prolonging the service life. Description of the Drawings
[0017] Figure 1 is a schematic structural diagram of the present utility model;
[0018] Figure 2 is a schematic top view structural diagram of the U-shaped seat of the present utility model;
[0019] Figure 3 is the present utility model Figure 1 A partial enlarged structural diagram of circle A.
[0020] Reference numerals: 1 - tank body, 10 - heating cavity, 101 - water injection pipe, 102 - drain pipe, 103 - heating resistance wire, 104 - support seat, 11 - feed inlet, 12 - discharge port, 2 - internal heating component, 20 - U-shaped seat, 200 - bearing, 21 - rotating cylinder, 210 - through hole, 211 - sealing ring, 212 - isolation plate, 213 - heater, 22 - stirring rod, 23 - scraping plate, 3 - rotating motor, 4 - rotating shaft, 5 - filter screen, 6 - heat preservation layer. Detailed Embodiments
[0021] The following combines embodiments and the drawings to further elaborate on the present utility model in detail, but the implementation manners of the present utility model are not limited thereto.
[0022] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "longitudinal", "lateral", "horizontal", "inner", "outer", "front", "rear", "top", "bottom", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the present utility model is customarily placed during use. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model.
[0023] In the description of the present utility model, it should also be noted that unless otherwise clearly specified and defined, the terms "set", "provided with", "installed", "connected", "coupled" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.
[0024] Embodiment 1
[0025] Embodiment 1 of the present utility model is an enzymatic hydrolysis device for protein polypeptide production, including a tank body 1 with a support base 104 and a discharge port 12 provided at the bottom. An inlet 11 is provided at the top of the tank body 1. An internal heating assembly 2 that rotates with a rotating shaft 4 is provided inside the tank body 1. A heating chamber 10 and a heat insulation layer 6 are sequentially provided on the outer periphery of the tank body 1.
[0026] Referring to Figure 1 , in this application, an internal heating assembly 2, a heating chamber 10 and a heat insulation layer 6 are respectively provided inside and outside the tank body 1. The internal heating assembly 2 rotates with the rotating shaft 4 to uniformly dissipate heat inside the tank body 1, so that the inside of the tank body 1 is in a uniform heating environment. At the same time, the heating chamber 10 plays a heating function on the reaction solution adjacent to the inner wall of the tank body 1, so that both the inner wall and the center of the tank body 1 are in a uniform heating environment, and continuous heat preservation is carried out under the action of the heat insulation layer 6, accelerating the enzymatic hydrolysis reaction rate inside the tank body 1 and being beneficial to reducing energy consumption.
[0027] In some preferred embodiments, the internal heating assembly 2 includes a rotating cylinder 21 and a heater 213 located inside the rotating cylinder 21. The rotating shaft 4 passes through the rotating cylinder 21. A U-shaped seat 20 is provided below the rotating cylinder 21. The bottom end of the U-shaped seat 20 is located at the bottom of the tank body 1. A bearing 200 is provided on the surface of the U-shaped seat 20. The bottom end of the rotating shaft 4 is located inside the bearing 200. The top end of the rotating shaft 4 is connected to the output end of the rotating motor 3. The bottom end of the U-shaped seat 20 is on the same vertical axis as the discharge port 12.
[0028] Referring to Figure 2 , the internal heater 213 assembly mainly includes a rotating cylinder 21 and a heater 213. The heater 213 is assembled inside the rotating cylinder 21 and connected to an external power supply. At the same time, the external power supply can control the heating temperature of the heater 213, which belongs to the prior art and will not be elaborated here.
[0029] Furthermore, the U-shaped seat 20 mainly provides a bottom mounting condition for the rotation of the rotating shaft 4, mainly for the bearing 200, so that the bottom end of the rotating shaft 4 rotates inside the bearing 200, thereby driving the entire rotating cylinder 21 and the heater 213 inside it to rotate. At the same time, the U-shaped seat 20 is on the same vertical axis as the discharge port 12, so that it does not affect the discharge of the reaction substances from the discharge port 12 after the protein polypeptide enzymatic hydrolysis reaction is completed.
[0030] In some preferred embodiments, a partition plate 212 is provided inside the rotating cylinder 21, and the partition plate 212 is adjacent to the rotating shaft 4. The main function of the partition plate 212 is to isolate the heater 213 and the rotating shaft 4, avoiding the heat generated by the heater 213 from directly transferring to the outer peripheral surface of the rotating shaft 4 and causing deformation. Both the partition plate 212 and the rotating shaft 4 are made of stainless steel, which is beneficial to extending the service life.
[0031] In some preferred embodiments, to ensure the connection sealing performance between the rotating shaft 4 and the rotating cylinder 21. Through holes 210 are respectively provided at the top and bottom of the rotating shaft 4. The rotating shaft 4 passes through the through holes 210, and a sealing ring 211 is provided between the through holes 210 and the rotating shaft 4. Referring to Figure 3 , the setting of the sealing ring 211 prevents the reaction liquid in the enzymatic hydrolysis reaction from entering the inside of the rotating cylinder 21, and the sealing ring 211 can be made of silicone material.
[0032] In some preferred embodiments, stirring rods 22 are respectively provided at the top of the rotating shaft 4, the middle part and the bottom of the rotating cylinder 21, and scraping plates 23 are provided at the ends of the stirring rods 22 adjacent to the inner wall of the tank body 1.
[0033] Meanwhile, the stirring rod 22 is connected to the rotating cylinder 21 and the rotating shaft 4, and can drive the stirring rod 22 and the scraper 23 to rotate during the rotation of the rotating shaft 4. The scraper 23 thus scrapes the inner wall and bottom of the tank body 1, enabling the reaction liquid adhering thereto to undergo a uniform enzymatic hydrolysis reaction, and avoiding errors in the reaction caused by excessive adhesion.
[0034] Embodiment 2
[0035] This Embodiment 2 is implemented on the basis of Embodiment 1. A water injection pipe 101 and a drain pipe 102 are respectively provided at the top and bottom of the heating cavity 10, and a plurality of heating resistance wires 103 are provided inside the heating cavity 10.
[0036] Specifically, the water injection pipe 101 and the drain pipe 102 penetrate through the heat insulation layer 6 and communicate with the heating cavity 10. At the same time, valves (not shown in the figure) are respectively provided. The heating resistance wires 103 are connected to an external power supply. After selecting the required heating temperature, heating is carried out to make the outside of the tank body 1 under the required temperature conditions. At the same time, under the combined action of the heater 213 in the internal heating assembly 2, uniform heating of the inner wall of the tank body 1 and the reaction liquid located at the center of the tank body 1 is achieved, improving the enzymatic hydrolysis rate.
[0037] Embodiment 3
[0038] This Embodiment 3 is implemented on the basis of Embodiment 1. A filter screen 5 is provided inside the discharge port 12. The filter screen 5 can filter impurities after the enzymatic hydrolysis reaction, reducing the impurity content of the enzymatic hydrolysis reactant after discharging. The specifications of the filter screen 5 are set according to the actual situation.
[0039] The working principle of the present utility model is as follows: When the reaction substances enter the inside of the tank body 1 through the feed port 11, the heater 213 and the heating resistance wires 103 are started through an external power supply for heating. At the same time, during the heating process, the rotary motor 3 is started. The output end of the rotary motor 3 causes the rotating shaft 4 to rotate under the assembly of the bearing 200 on the surface of the U-shaped seat 20, driving the rotating cylinder 21 and the heater 213 inside it to rotate, realizing uniform heating inside the tank body 1. And the filter screen 5 provided inside the discharge port 12 is beneficial to reducing the impurity content of the enzymatic hydrolysis reactant after discharging; the partition plate 212 provided inside the rotating cylinder 21 prevents the heat of the heater 213 from directly being transferred into the rotating shaft 4, preventing the rotating shaft 4 from deforming; a sealing ring 211 is provided between the rotating shaft 4 and the through hole 210 to prevent the reaction liquid inside the tank body 1 from entering the inside of the rotating cylinder 21 during the enzymatic hydrolysis process, preventing the heater 213 from being damaged.
[0040] The above is only a preferred embodiment of the present utility model, and does not impose any form of limitation on the present utility model. Based on the technical essence of the present utility model, any simple modifications, equivalent replacements, and improvements made to the above embodiments within the spirit and principles of the present utility model still fall within the protection scope of the technical solution of the present utility model.
Claims
1. An enzymatic hydrolysis device for protein polypeptide production, characterized in that: The invention comprises a tank body (1) having a support seat (104) and a discharge port (12) at the bottom, a feed port (11) at the top of the tank body (1), an internal heating component (2) rotating with a rotating shaft (4) inside the tank body (1), and a heating chamber (10) and a heat-insulating layer (6) in sequence on the outer periphery of the tank body (1).
2. The enzymatic hydrolysis device for protein polypeptide production according to claim 1, characterized in that: The internal heating component (2) comprises a rotating cylinder (21) and a heater (213) located inside the rotating cylinder (21), and the rotating shaft (4) passes through the rotating cylinder (21).
3. The enzymatic hydrolysis device for protein polypeptide production according to claim 2, characterized in that: A U-shaped seat (20) is provided below the rotating cylinder (21), the bottom end of the U-shaped seat (20) is located at the bottom of the tank body (1), a bearing (200) is provided on the surface of the U-shaped seat (20), the bottom end of the rotating shaft (4) is located in the bearing (200), and the top end of the rotating shaft (4) is connected to the output end of the rotating motor (3).
4. The enzymatic hydrolysis device for protein polypeptide production according to claim 3, characterized in that: The bottom end of the U-shaped seat (20) and the discharge port (12) are located on the same vertical axis.
5. The enzymatic hydrolysis device for protein polypeptide production according to claim 3, characterized in that: An isolation plate (212) is provided inside the rotating cylinder (21), and the isolation plate (212) is adjacent to the rotating shaft (4).
6. The enzymatic hydrolysis device for protein polypeptide production according to claim 3, characterized in that: The top and bottom of the rotating shaft (4) are respectively provided with through holes (210), the rotating shaft (4) passes through the through hole (210), and a sealing ring (211) is provided between the through hole (210) and the rotating shaft (4).
7. The enzymatic hydrolysis device for protein polypeptide production according to claim 3, characterized in that: A stirring rod (22) is provided at the top of the rotating shaft (4), the middle and the bottom of the rotating cylinder (21), respectively. A scraper (23) is provided at the end of the stirring rod (22) adjacent to the inner wall of the tank body (1).
8. The enzymatic hydrolysis device for protein polypeptide production according to claim 3, characterized in that: A water injection pipe (101) and a drainage pipe (102) are respectively provided at the top and bottom of the heating chamber (10), and a plurality of heating resistance wires (103) are provided inside the heating chamber (10).
9. The enzymatic hydrolysis device for protein polypeptide production according to claim 3, characterized in that: A filter screen (5) is provided in the discharge port (12).