Anti-inclination electrophoresis comb

Abstract

The utility model provides an anti-tilt electrophoresis comb, which belongs to the technical field of electrophoresis combs and comprises an electrophoresis clamping groove, a comb and a stabilizing sleeve, the electrophoresis clamping groove is of an H-shaped structure and is used for supporting the comb so that the comb is placed perpendicular to the direction of an electric field; the combs are arranged on the upper side and the lower side of the electrophoresis clamping groove and are used for penetrating through the gel plate so as to separate samples in an electric field; the four corners of the H shape of the electrophoresis clamping groove are provided with the stabilizing sleeves, and the stabilizing sleeves are used for preventing the electrophoresis clamping groove and the comb from shaking in the glue pouring and glue solidification process, so that glue holes are too large. The comb comprises a first sample adding hole reserved plate and a second sample adding hole reserved plate; the utility model provides an anti-inclination electrophoresis comb which can solve the problems that an existing electrophoresis comb is prone to inclination and shaking in the using process, stability is insufficient, glue holes are too large, and experiment results are affected.

Description

Technical Field

[0001] The utility model belongs to the technical field of electrophoresis combs, and more specifically, relates to an anti-tilting electrophoresis comb. Background Art

[0002] An electrophoresis comb is a laboratory technical tool used for separating and purifying DNA, RNA, or proteins. It utilizes the action of an electric field on charged particles to separate them. In an electrophoresis comb, there is usually a flat plate with grooves. A buffer solution is injected into the grooves, and then an electric field is applied at both ends. Samples are loaded into the grooves, and due to the influence of the electric field, charged molecules will separate in the grooves according to their different sizes and charge migration rates. Electrophoresis combs are widely used in molecular biology and biochemistry research, such as in fields like DNA fingerprint analysis, protein separation and purification, etc. By controlling the migration rate of charged particles, the separation and purification of target molecules can be achieved. Electrophoresis combs can also be used to analyze the components and characteristics of samples. For example, by observing the positions and intensities of DNA bands or protein bands, the composition and concentration of samples can be understood.

[0003] Existing electrophoresis combs are prone to tilting and shaking during use, with insufficient stability, resulting in too large gel pores and affecting experimental results. Summary of the Utility Model

[0004] In view of this, the utility model provides an anti-tilting electrophoresis comb, which can solve the problems that existing electrophoresis combs are prone to tilting and shaking during use, with insufficient stability, resulting in too large gel pores and affecting experimental results.

[0005] The utility model is implemented as follows:

[0006] The utility model provides an anti-tilting electrophoresis comb, which includes an electrophoresis card slot, a comb, and a stabilizing sleeve. The electrophoresis card slot is of an H-shaped structure and is used to support the comb so that the comb is placed perpendicular to the direction of the electric field. The combs are arranged on the upper and lower sides of the electrophoresis card slot and are used to penetrate the gel plate so that samples can be separated in the electric field. The stabilizing sleeves are arranged at the four corners of the H shape of the electrophoresis card slot, and the stabilizing sleeves are used to prevent the electrophoresis card slot and the comb from shaking during the process of pouring the gel and the gel solidifying, resulting in too large gel pores.

[0007] The technical effects of the anti-tilting electrophoresis comb provided by the utility model are as follows: The electrophoresis card slot is of an H-shaped structure, which supports the comb to be placed perpendicular to the direction of the electric field. The combs are located on the upper and lower sides of the electrophoresis card slot and are used to penetrate the gel plate for sample separation. The stabilizing sleeves are arranged at the corners of the electrophoresis card slot to prevent shaking during the process of pouring the gel and the gel solidifying, resulting in too large gel pores.

[0008] On the basis of the above technical solution, the anti-tilt electrophoresis comb of the present utility model can be further improved as follows:

[0009] Among them, the comb includes a first sample loading hole reservation plate and a second sample loading hole reservation plate. The first sample loading hole reservation plate and the second sample loading hole reservation plate are arranged in parallel on the upper and lower sides of the middle position of the electrophoresis card slot. The first sample loading hole reservation plate and the second sample loading hole reservation plate are both provided with a plurality of parallel teeth, and the distances between adjacent teeth of the first sample loading hole reservation plate are the same.

[0010] Further, the second sample loading hole reservation plate includes two groups of teeth with the same quantity, and the distances between adjacent teeth within the two groups of teeth are the same; the teeth of the first sample loading hole reservation plate and the teeth of the second sample loading hole reservation plate have the same length.

[0011] Further, a gap is provided between the two groups of teeth of the second sample loading hole reservation plate, and the gap is used for positioning.

[0012] The beneficial effects of adopting the above improvement scheme are as follows: The comb includes a first sample loading hole reservation plate and a second sample loading hole reservation plate, which are arranged in parallel on the upper and lower sides of the middle position of the electrophoresis card slot. These reservation plates are provided with a plurality of parallel teeth for sample loading. The second sample loading hole reservation plate further includes two groups of teeth, the distances between adjacent teeth are the same, the teeth have the same length as those of the first sample loading hole reservation plate, and there is a gap between the two groups of teeth for positioning.

[0013] Further, there are 4 stabilizing sleeves, which are respectively sleeved on the four corners of the electrophoresis card slot. The 4 stabilizing sleeves have the same length, and the thickness is set according to the surface on which the electrophoresis comb is placed. The top surfaces of the 4 stabilizing sleeves after being sleeved on the 4 corners of the electrophoresis card slot are located on the same horizontal plane.

[0014] Further, the inner groove structure of the stabilizing sleeve is the same as the structures of the four corners of the electrophoresis card slot, and the inner diameter of the stabilizing sleeve is the same as the outer diameters of the four corners of the electrophoresis card slot.

[0015] Further, a plurality of parallel protrusions are provided at the four corner positions of the electrophoresis card slot. The protrusions are in a horizontal structure, and fixing grooves are correspondingly provided inside the stabilizing sleeve, and the fixing grooves are adapted to the protrusions.

[0016] Further, scale values are provided on the plurality of parallel protrusions, and the scale values are used to display the depth at which the stabilizing sleeve is sleeved on the four corners of the electrophoresis card slot.

[0017] Further, the upper and lower surfaces of the stabilizing sleeve are in a planar structure.

[0018] Further, the stabilizing sleeve is made of rubber material, which is used to reduce vibration and increase friction.

[0019] The beneficial effects of adopting the above improvement scheme are as follows: There are 4 stabilizing sleeves, which are sleeved on the four corners of the electrophoresis slot, with the same length, and the inner groove structure is the same as that of the corner of the electrophoresis slot. There are also multiple parallel protrusions on the four corners of the electrophoresis slot, and corresponding fixing grooves are arranged inside the stabilizing sleeves. Scale values are set on the multiple parallel protrusions to display the depth at which the stabilizing sleeve is sleeved on the electrophoresis slot. The upper and lower surfaces of the stabilizing sleeve are flat structures, and the material is rubber, which is used to reduce vibration and increase friction.

[0020] Compared with the prior art, the beneficial effects of an anti-tilting electrophoresis comb provided by the present utility model are as follows:

[0021] Electrophoresis slot (H-shaped structure): The effect of this component is to support the comb so that it can be placed perpendicular to the direction of the electric field. The electrophoresis slot also provides a platform for the samples to be separated in the electric field. Its H-shaped structure can provide stability to prevent adverse effects caused by the comb tilting or shaking.

[0022] Comb: The combs are arranged on the upper and lower sides of the electrophoresis slot. Their effect is to penetrate the gel plate and allow the samples to be separated in the electric field. These combs usually have reserved hole plates, including the first and second sample loading hole reserved plates. The teeth on these reserved plates help to position the samples and ensure their correct separation during electrophoresis.

[0023] Stabilizing sleeve: The stabilizing sleeves are installed on the four corners of the H-shaped structure of the electrophoresis slot. Their effect is to prevent the electrophoresis slot and the comb from shaking during the process of pouring the gel and the gel solidifying, which may cause the gel holes to be too large. The stabilizing sleeves usually match the corner structure of the electrophoresis slot to ensure stability. In addition, the scale values of the stabilizing sleeves can be used to display the depth at which they are sleeved on the corners of the electrophoresis slot, thereby providing more precise installation.

[0024] The design of the comb is optimized so that the first and second sample loading hole reserved plates are arranged in parallel on the upper and lower sides of the middle position of the electrophoresis slot. This helps to better position the samples and ensure correct separation.

[0025] A gap is provided between the two groups of teeth of the second sample loading hole reserved plate for better positioning.

[0026] The improvement of the stabilizing sleeve includes four sets, and each set is sleeved on the four corners of the electrophoresis slot to provide more stable support.

[0027] The material of the stabilizing sleeve is selected as rubber to reduce vibration and increase friction, thereby further improving stability. Description of the Drawings

[0028] To more clearly illustrate the technical solutions of the embodiments of the present utility model, the following will briefly introduce the drawings required for use in the description of the embodiments of the present utility model. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0029] Figure 1 The specific structure of an anti-tilting electrophoresis comb;

[0030] In the drawings, the list of components represented by each reference numeral is as follows:

[0031] 10. Electrophoresis card slot; 20. Comb; 21. First sample loading hole reservation plate; 22. Second sample loading hole reservation plate; 30. Stabilizing sleeve. Specific embodiments

[0032] To make the purpose, technical solutions, and advantages of the embodiments of the present utility model clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present utility model with reference to the drawings in the embodiments of the present utility model.

[0033] As Figure 1 shown, it is an embodiment of an anti-tilting electrophoresis comb provided by the present utility model. In this embodiment, it includes an electrophoresis card slot 10, a comb 20, and a stabilizing sleeve 30. The electrophoresis card slot 10 is of an H-shaped structure and is used to support the comb 20 so that the comb 20 is placed perpendicular to the electric field direction; the comb 20 is arranged on the upper and lower sides of the electrophoresis card slot 10 and is used to penetrate the gel plate so that the samples are separated in the electric field; stabilizing sleeves 30 are arranged at the four corners of the H shape of the electrophoresis card slot 10, and the stabilizing sleeves 30 are used to prevent the electrophoresis card slot 10 and the comb 20 from shaking during the process of pouring the gel and the gel solidifying, resulting in too large gel holes.

[0034] Among them, in the above technical solution, the comb 20 includes a first sample loading hole reservation plate 21 and a second sample loading hole reservation plate 22. The first sample loading hole reservation plate 21 and the second sample loading hole reservation plate 22 are arranged in parallel on the upper and lower sides of the middle position of the electrophoresis card slot 10. The first sample loading hole reservation plate 21 and the second sample loading hole reservation plate 22 are both provided with a number of parallel teeth, and the distances between adjacent teeth of the first sample loading hole reservation plate 21 are the same.

[0035] Furthermore, in the above technical solution, the second sample loading hole reservation plate 22 includes two groups of teeth with the same quantity, and the distances between adjacent teeth within the two groups of teeth are the same; the lengths of the teeth of the first sample loading hole reservation plate 21 and the teeth of the second sample loading hole reservation plate 22 are the same.

[0036] Furthermore, in the above technical solution, a gap is provided between the two groups of teeth of the second sample loading hole reservation plate 22, and the gap is used for positioning.

[0037] Furthermore, in the above technical solution, there are 4 stabilizing sleeves 30, which are respectively sleeved on the four corners of the electrophoresis slot 10. The 4 stabilizing sleeves 30 have the same length, and the thickness is set according to the surface where the electrophoresis comb is placed. The top surfaces of the 4 stabilizing sleeves 30 after being sleeved on the four corners of the electrophoresis slot 10 are located on the same horizontal plane.

[0038] Furthermore, in the above technical solution, the inner groove structure of the stabilizing sleeve 30 is the same as the structures of the four corners of the electrophoresis slot 10, and the inner diameter of the stabilizing sleeve 30 is the same as the outer diameters of the four corners of the electrophoresis slot 10.

[0039] Furthermore, in the above technical solution, a plurality of parallel protrusions are provided at the four corner positions of the electrophoresis slot 10. The protrusions are in a horizontal structure, and fixing grooves are correspondingly provided inside the stabilizing sleeve 30, and the fixing grooves are adapted to the protrusions.

[0040] Furthermore, in the above technical solution, scale values are provided on the plurality of parallel protrusions, and the scale values are used to show the depth at which the stabilizing sleeve 30 is sleeved on the four corners of the electrophoresis slot 10.

[0041] During use, prepare the sample and the electrophoresis buffer solution; select stabilizing sleeves with appropriate thickness according to the height of the plane, respectively adjust the installation distances between the stabilizing sleeves and the four corners of the electrophoresis slot, sleeve the stabilizing sleeves on the four corners of the electrophoresis slot, and ensure their firm connection with the slot; use the first and second sample loading hole reservation plates for sample loading respectively; place the electrophoresis comb on the gel plate and ensure that the comb is placed perpendicular to the electric field direction; start the electrophoresis process to separate the sample in the electric field; after the process is completed, take out the gel plate and perform subsequent experimental operations.

[0042] Furthermore, in the above technical solution, the upper and lower surfaces of the stabilizing sleeve 30 are in a planar structure.

[0043] Furthermore, in the above technical solution, the stabilizing sleeve 30 is made of rubber material, which is used to reduce vibration and increase friction.

[0044] Specifically, the principle of the present utility model is: prepare the sample and the electrophoresis buffer solution; select stabilizing sleeves with appropriate thickness according to the height of the plane, respectively adjust the installation distances between the stabilizing sleeves and the four corners of the electrophoresis slot, sleeve the stabilizing sleeves on the four corners of the electrophoresis slot, and ensure their firm connection with the slot; use the first and second sample loading hole reservation plates for sample loading respectively; place the electrophoresis comb on the gel plate and ensure that the comb is placed perpendicular to the electric field direction; start the electrophoresis process to separate the sample in the electric field; after the process is completed, take out the gel plate and perform subsequent experimental operations.

Claims

1. An anti-tilting electrophoresis comb, characterized in that It includes an electrophoresis slot (10), a comb (20) and a stabilizing sleeve (30). The electrophoresis slot (10) is of an H-shaped structure and is used to support the comb (20) so that the comb (20) is placed perpendicular to the electric field direction. The combs (20) are arranged on the upper and lower sides of the electrophoresis slot (10) and are used to penetrate the gel plate so that samples can be separated in the electric field. The stabilizing sleeves (30) are arranged at the four corners of the H shape of the electrophoresis slot (10), and the stabilizing sleeves (30) are used to prevent the electrophoresis slot (10) and the comb (20) from shaking during the process of pouring the gel and the gel solidifying, which may cause the gel holes to be too large.

2. The anti-tilt electrophoresis comb according to claim 1, characterized in that, The comb (20) includes a first sample loading hole reservation plate (21) and a second sample loading hole reservation plate (22). The first sample loading hole reservation plate (21) and the second sample loading hole reservation plate (22) are arranged in parallel on the upper and lower sides of the middle position of the electrophoresis slot (10). The first sample loading hole reservation plate (21) and the second sample loading hole reservation plate (22) are both provided with a number of parallel teeth, and the distances between adjacent teeth of the first sample loading hole reservation plate (21) are the same.

3. The anti-tilting electrophoresis comb according to claim 2, characterized in that, The second sample loading hole reservation plate (22) includes two groups of teeth with the same quantity, and the distances between adjacent teeth within the two groups of teeth are the same. The teeth of the first sample loading hole reservation plate (21) and the teeth of the second sample loading hole reservation plate (22) are of the same length.

4. The anti-tilting electrophoresis comb according to claim 3, characterized in that, A gap is arranged between the two groups of teeth of the second sample loading hole reservation plate (22), and the gap is used for positioning.

5. The anti-tilting electrophoresis comb according to claim 4, characterized in that There are 4 stabilizing sleeves (30), which are respectively sleeved on the four corners of the electrophoresis slot (10). The lengths of the 4 stabilizing sleeves (30) are the same, and the thickness is set according to the surface where the electrophoresis comb is placed. The top surfaces of the 4 stabilizing sleeves (30) sleeved on the 4 corners of the electrophoresis slot (10) are located on the same horizontal plane.

6. The anti-tilting electrophoresis comb according to claim 5, wherein, The inner groove structure of the stabilizing sleeve (30) is the same as the structure of the four corners of the electrophoresis slot (10), and the inner diameter of the stabilizing sleeve (30) is the same as the outer diameter of the four corners of the electrophoresis slot (10).

7. The anti-tilting electrophoresis comb according to claim 6, wherein, A plurality of parallel protrusions are arranged at the four corner positions of the electrophoresis slot (10). The protrusions are of a transverse structure, and fixing grooves are correspondingly arranged inside the stabilizing sleeve (30), and the fixing grooves are adapted to the protrusions.

8. The anti-tilting electrophoresis comb according to claim 7, characterized in that, Scale values are arranged on the plurality of parallel protrusions, and the scale values are used to display the depth at which the stabilizing sleeve (30) is sleeved on the four corners of the electrophoresis slot (10).

9. The anti-tilting electrophoresis comb according to claim 8, characterized in that, The upper and lower surfaces of the stabilizing sleeve (30) are of a planar structure.

10. The anti-tilt electrophoresis comb according to claim 9, wherein The stabilizing sleeve (30) is made of rubber material and is used to reduce vibration and increase friction.

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