Cell interception bottle

Abstract

A cell interception bottle comprises an interception bottle body, a long pipe, a first short pipe and a second short pipe, the long pipe, the first short pipe and the second short pipe are inserted into the interception bottle body, an interception net is arranged in the interception bottle body, a bottle cap is arranged on the interception bottle body, a pipeline fixing plate is arranged in the bottle cap, and the bottom of the long pipe penetrates through the interception net; the cell interception bottle disclosed by the utility model can realize low-cost sterile and rapid separation of cell supernatant and cell sap in a continuous culture stage or a large-scale culture stage, and also can realize the regulation and control effect of cell density.

Description

Technical Field

[0001] The utility model relates to the technical field of aseptic cell culture, in particular to a cell retention bottle. Background Art

[0002] In sterile cell culture, sterile separation and recovery of cells and cell supernatant can only be achieved through sterile centrifugation or hollow fiber membranes. Centrifugation is used to separate cells from cell supernatant, which requires aseptic transfer of cell fluid. This method is convenient and fast for small volumes, but when the volume exceeds 0.5L, recovery by centrifugation becomes time-consuming and labor-intensive. Another separation method, hollow fiber membranes, is based on alternating tangential flow technology to achieve continuous separation and recovery of cells and supernatant. This method has the advantage of continuous operation, but it relies on expensive equipment and has high requirements for a sterile environment and operator skills, making it not suitable for all laboratories. Utility Model Content

[0003] The technical problem to be solved by the utility model is: in order to solve the problem that aseptic separation and cell recovery in the prior art are time-consuming and labor-intensive, the utility model provides a cell retention bottle.

[0004] The technical solution adopted by the utility model to solve its technical problems is: a cell retention bottle, including a retention bottle body and a long tube, a first short tube and a second short tube inserted into the retention bottle body, a retention net is provided inside the retention bottle body, a bottle cap is provided on the retention bottle body, a pipeline fixing plate is provided in the bottle cap, and the bottom of the long tube passes through the retention net.

[0005] Furthermore, the bottle cap is an annular structure, and a first through hole, a second through hole and a third through hole are provided on the pipeline fixing plate. One end of the long tube passes through the first through hole and enters the retention bottle body, one end of the first short tube passes through the second through hole and enters the retention bottle body, and one end of the second short tube passes through the third through hole and enters the retention bottle body.

[0006] Furthermore, the end of the long tube away from the retention bottle body is connected to an adapter, and the supernatant recovery system and the fluid replenishment system are externally connected through the adapter; the supernatant recovery system can be a membrane filter to realize the recovery of sterile cell supernatant, and the fluid replenishment system is a sterile culture medium or cleaning fluid, which can realize the functions of cell resuspension and filter flushing.

[0007] Furthermore, the end of the first short tube that enters the retention bottle body is located above the retention net, and the end away from the retention bottle body is connected to the adapter, and the adapter is used to connect to the external cell recovery system and the sterile capture system; the cell recovery system uses a culture device or a sterile cell bottle to realize cell transfer recovery and cell removal, and the sterile capture system uses a sterile culture bottle or reactor to realize the transfer of sterile cell fluid or the replenishment of selected cells.

[0008] Furthermore, the second short tube is a short tube that passes through the bottle mouth, and the end of the second short tube that enters the retention bottle body is below the bottle mouth. The end of the second short tube away from the retention bottle body is connected to the adapter, and the adapter is used to connect the external balancing system. The balancing system uses an air filter membrane, an air pump or a vacuum filtration pump to balance the air pressure in the bottle, thereby achieving high pressure or vacuum in the bottle.

[0009] Furthermore, the retention net is arranged at 1 / 5-1 / 3 away from the bottom of the retention bottle body, which is convenient for effectively separating the cell supernatant from the cells. The retention bottle body is provided with multiple groups of protrusions at 1 / 5 to 1 / 3 away from the bottom of the bottle to support the retention net; the protrusions are provided to support the retention net to prevent it from falling downward.

[0010] Furthermore, the retention net is provided with a fourth through hole for convenient passage of the long tube, a filter screen or filter paper is placed above the retention net, and grooves matching the raised portion are provided around the retention net; the retention net can be a micron-level filter screen or filter paper, or a nano-level filter screen or filter paper can be installed, which can be sterilized at high temperatures and used multiple times, and grooves matching the raised portion are provided around the retention net to facilitate adjustment of the placement height of the retention net. When the groove corresponds to the raised portion, the retention net can be moved up and down. After moving to the appropriate position, the retention net can be rotated, and the groove can be rotated between the raised portions to fix the retention net there to prevent it from falling.

[0011] Furthermore, the retention bottle body is made of metal, glass or high temperature resistant plastic.

[0012] Beneficial effects of the utility model:

[0013] The cell retention bottle of the utility model can achieve low-cost, aseptic and rapid separation of cell supernatant and cell fluid in a continuous culture stage or a large-scale culture stage, and can also achieve the regulation of cell density. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0015] Figure 1 This is a schematic diagram of a cell retention bottle according to a preferred embodiment of the present invention;

[0016] Figure 2 This is a top view of the pipeline fixing plate according to the preferred embodiment of the present utility model;

[0017] Figure 3 A schematic diagram of the interception net according to a preferred embodiment of the present invention;

[0018] Figure 4This is a schematic diagram of the retention bottle body according to the preferred embodiment of the present utility model;

[0019] In the figure, 1. retention bottle body, 2. long tube, 3. first short tube, 4. second short tube, 5. retention net, 6. bottle cap, 7. first through hole, 8. second through hole, 9. third through hole, 10. fourth through hole, 11. pipeline fixing plate, 12. protrusion, 13. groove. DETAILED DESCRIPTION

[0020] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention.

[0021] In the description of the present invention, it should be understood that the terms "upper", "lower", "horizontal", "top", "inside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0022] In the description of this utility model, it should be noted that, unless otherwise specified or limited, the term "connection" should be understood in a broad sense. For example, it can mean fixed connection, detachable connection, or integral connection; it can mean mechanical connection or electrical connection; it can mean direct connection or indirect connection through an intermediary. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0023] like Figure 1-2 As shown, a cell retention bottle includes a retention bottle body 1, a long tube 2, a first short tube 3, and a second short tube 4 inserted into the retention bottle body 1. The retention bottle body 1 is provided with a retention net 5 inside. The retention bottle body 1 is provided with a bottle cap 6, and a pipeline fixing plate 11 is provided in the bottle cap 6. The bottom of the long tube 2 passes through the retention net 5. The bottle cap 6 is annular in structure. The pipeline fixing plate 11 is provided with a first through hole 7, a second through hole 8, and a third through hole 9. One end of the long tube 2 passes through the first through hole 7 to enter the retention bottle body 1, one end of the first short tube 3 passes through the second through hole 8 to enter the retention bottle body 1, and one end of the second short tube 4 passes through the third through hole 9 to enter the retention bottle body 1.

[0024] The end of the long tube 2 away from the retention bottle body 1 is connected to the adapter, and the supernatant recovery system and the fluid replenishment system are externally connected through the adapter; the supernatant recovery system can be a membrane filter to realize the recovery of sterile cell supernatant, and the fluid replenishment system is a sterile culture medium or cleaning fluid, which can realize the functions of cell resuspension and filter flushing.

[0025] The end of the first short tube 3 entering the retention bottle body 1 is located above the retention net 5, and the end away from the retention bottle body 1 is connected to the adapter, and the external cell recovery system and the sterile capture system are connected through the adapter; the cell recovery system adopts a culture device or a sterile cell bottle to realize cell transfer recovery and cell removal, and the sterile capture system adopts a sterile culture bottle or reactor to realize the transfer of sterile cell fluid or the replenishment of selected cells.

[0026] The second short tube 4 is a short tube that passes through the bottle mouth. The end of the second short tube 4 that enters the retention bottle body 1 is below the bottle mouth. The end of the second short tube 4 that is away from the retention bottle body 1 is connected to the adapter, and the adapter is used to connect to the external balancing system. The balancing system uses an air filter membrane, an air pump or a vacuum filtration pump to balance the air pressure in the bottle, thereby achieving high pressure or vacuum in the bottle.

[0027] The retention net 5 is arranged at 1 / 5-1 / 3 of the distance from the bottom of the retention bottle body 1, which is convenient for effectively separating the cell supernatant from the cells. The retention bottle body 1 is provided with multiple groups of protrusions 12 at 1 / 5 to 1 / 3 of the distance from the bottom of the bottle to support the retention net 5; the protrusions 12 are provided to support the retention net 5 to prevent it from falling downward. The interception net 5 is provided with a fourth through hole 10 for the long tube 2 to pass through. A filter screen or filter paper is placed above the interception net 5. The interception net 5 is provided with grooves 13 matching the raised portion 12 around it. The interception net 5 can be a micron-level filter screen or filter paper, or a nano-level filter screen or filter paper. It can be sterilized at high temperatures and used multiple times. The interception net 5 is provided with grooves 13 matching the raised portion 12 around it, which can facilitate the adjustment of the placement height of the interception net 5. When the grooves 13 correspond to the raised portion 12, the interception net 5 can be moved up and down. After moving to the appropriate position, the interception net 5 can be rotated and the grooves 13 can be rotated between the raised portions 12 to fix the interception net 5 there to prevent it from falling. The interception bottle body 1 is made of metal, glass, or high-temperature resistant plastic.

[0028] When in use, align the groove 13 of the interception net 5 with the raised portion 12, then move the position of the interception net 5 to 1 / 3 of the distance from the bottom of the bottle, and rotate the interception net 5 to fix it on the raised portion 12; you can choose a filter screen or filter paper with a pore size of 20um or less, make it into a size similar to the interception net and fix it on the interception net, then place the pipeline and the pipeline fixing plate 11 on the interception bottle body 1, tighten the bottle cap 6, and then it can be used. After assembly, connect it to a continuous culture device such as a reactor for sterilization according to actual needs. If filter paper is used as a interception, it needs to be covered with water for sterilization; if it is used alone, the external connection pipeline needs to be connected to the filter air valve for high-pressure and high-temperature sterilization, and then it can be aseptically connected to the reactor through a sterile welding machine.

[0029] In this specification, the schematic representations of the terms do not necessarily refer to the same embodiment. Moreover, the specific features, structures, materials or characteristics described can be combined in any suitable manner in any one or more embodiments.

[0030] Based on the above-mentioned ideal embodiment of the present invention, and in accordance with the above description, relevant personnel can make various changes and modifications without departing from the technical scope of the present invention. The technical scope of the present invention is not limited to the content of the specification, but must be determined according to the scope of the claims.

Claims

1. A cell retention bottle, characterized in that: The invention comprises a retention bottle body (1), a long tube (2), a first short tube (3), and a second short tube (4) inserted into the retention bottle body (1); a retention net (5) is provided inside the retention bottle body (1); a bottle cap (6) is provided on the retention bottle body (1); a pipeline fixing plate (11) is provided inside the bottle cap (6); and the bottom of the long tube (2) passes through the retention net (5).

2. The cell retention bottle according to claim 1, characterized in that The bottle cap (6) is an annular structure. The pipeline fixing plate (11) is provided with a first through hole (7), a second through hole (8) and a third through hole (9). One end of the long tube (2) passes through the first through hole (7) and enters the retention bottle body (1). One end of the first short tube (3) passes through the second through hole (8) and enters the retention bottle body (1). One end of the second short tube (4) passes through the third through hole (9) and enters the retention bottle body (1).

3. The cell retention bottle according to claim 1, characterized in that One end of the long tube (2) away from the interception bottle body (1) is connected to an adapter, and is externally connected to a supernatant recovery system and a liquid replenishment system via the adapter.

4. The cell retention bottle according to claim 1, characterized in that The end of the first short tube (3) that enters the retention bottle body (1) is located above the retention net (5), and the end away from the retention bottle body (1) is connected to the adapter, and the cell recovery system and the sterile capture system are connected to the adapter.

5. The cell retention bottle according to claim 1, characterized in that The second short tube (4) is a short tube passing through the bottle mouth. The end of the second short tube (4) entering the retention bottle body (1) is below the bottle mouth. The end of the second short tube (4) away from the retention bottle body (1) is connected to the adapter, and the balancing system is connected to the adapter.

6. The cell retention bottle according to claim 1, characterized in that The interception net (5) is arranged at a distance of 1 / 5 to 1 / 3 from the bottom of the interception bottle body (1); the interception bottle body (1) is provided with a plurality of groups of protrusions (12) at a distance of 1 / 5 to 1 / 3 from the bottom of the bottle for supporting the interception net (5).

7. The cell retention bottle according to claim 6, characterized in that The interception net (5) is provided with a fourth through hole (10) for the long tube (2) to pass through. A filter screen or filter paper is placed above the interception net (5). The interception net (5) is provided with grooves (13) matching the raised portion (12) around it.

8. The cell retention bottle according to claim 1, characterized in that The interception bottle body (1) is made of metal, glass or high-temperature resistant plastic.

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