Nematode suction device

Abstract

The utility model relates to the technical field of plant quarantine, in particular to a nematode suction device. Comprising a vacuum exhaust pipe, a connecting pipe and a glass suction pipe which are sequentially connected from top to bottom, the upper portion of the connecting pipe is sleeved with a glass sleeve, the glass sleeve is sleeved with a rubber sleeve, the upper end of the rubber sleeve is tightly sleeved with the lower end of the vacuum exhaust pipe, so that the vacuum exhaust pipe and the connecting pipe are fixedly connected into a whole, and the lower end of the connecting pipe is sleeved with a grid. The lower end of the grid is connected with the nematode adhesive film, and the glass suction pipe is connected to the lower portion of the connecting pipe in a sleeved mode and located between the rubber sleeve and the connecting pipe. The nematode suction device is reasonable in structure, ingenious in design, simple, convenient and easy to operate, an inexperienced person can suck nematodes easily, rapidly and efficiently, and the success rate of follow-up experiments and the overall working efficiency are greatly improved.

Description

Technical Field

[0001] This utility model relates to the field of plant quarantine technology, and in particular to a nematode extractor. Background Technology

[0002] In plant quarantine work, nematode identification is necessary. Since the isolated nematodes are in water, they must be separated from the water and placed on a glass slide to prepare a temporary slide for identification. Therefore, the preparation of nematode slide specimens and molecular biology experiments both require the nematodes to be separated from the water before proceeding. Current methods for separating nematodes from water involve using a cautery needle, but this requires experience and skill, and is difficult for beginners. This is especially true in molecular biology experiments, where nematodes need to be transferred to centrifuge tubes containing sterile water. Because nematodes are invisible to the naked eye, it's impossible to confirm their presence in the centrifuge tube. Often, the failure to pick up nematodes with the cautery needle leads to the failure of the final molecular biology experiment, wasting time and resulting in low efficiency. Utility Model Content

[0003] This invention addresses the problems existing in the prior art by providing a nematode extractor that requires little operator experience, is simple to operate, and has a high nematode extraction rate.

[0004] The technical solution adopted by this utility model to achieve the above-mentioned objective is as follows: a nematode extractor, characterized in that it includes a vacuum extraction tube, a connecting tube, and a glass suction tube connected sequentially from top to bottom. The upper part of the connecting tube is fitted with a glass sleeve, and the glass sleeve is fitted with a rubber sleeve. The upper end of the rubber sleeve is tightly fitted to the lower end of the vacuum extraction tube, so that the vacuum extraction tube and the connecting tube are fixedly connected as one unit. The lower end of the connecting tube is fitted with a mesh, and the lower end of the mesh is connected to the nematode adhesive membrane. The glass suction tube is fitted to the lower part of the connecting tube and is located between the rubber sleeve and the connecting tube.

[0005] The upper and lower parts of the connecting tube are connected to a limiting protrusion. The upper part of the limiting protrusion is fixedly connected to a glass sleeve, and the lower part is detachably connected to a glass straw.

[0006] The vacuum extraction tube includes a tube body and a piston connected inside the tube. The upper part of the tube body is a piston chamber, and the lower part is a transition tube with an inner diameter smaller than that of the piston chamber. A rubber sleeve is tightly fitted on the transition tube. One end of the piston is located outside the piston chamber to allow for the extraction of liquid.

[0007] The mesh is a 60-80 mesh stainless steel mesh, and the nematode adhesive membrane is a 300-500 mesh disposable nylon film.

[0008] This invention features a reasonable structure and ingenious design, enabling even inexperienced individuals to easily, quickly, and efficiently extract nematodes. It is simple to operate and greatly improves the success rate of subsequent experiments and overall work efficiency. Attached Figure Description

[0009] Figure 1 This is a schematic diagram of the structure of this utility model;

[0010] Figure 2 This is an enlarged view of the mesh and nematode adhesive membrane structure;

[0011] In the diagram: 1. Vacuum extraction tube, 2. Rubber sleeve, 3. Glass sleeve, 4. Connecting tube, 5. Mesh, 6. Nematode adhesive membrane, 7. Glass straw. Detailed Implementation Example 1

[0012] like Figures 1-2 The nematode extractor shown includes a vacuum extraction tube 1, a connecting tube 4, and a glass pipette 7 connected sequentially from top to bottom. A glass sleeve 3 is fitted onto the upper part of the connecting tube 4, and a rubber sleeve 2 is fitted onto the outer sleeve of the glass sleeve 3. The upper end of the rubber sleeve 2 is tightly fitted onto the lower end of the vacuum extraction tube 1, so that the vacuum extraction tube 1 and the connecting tube 4 are fixedly connected as one unit. A mesh 5 is fitted onto the lower end of the connecting tube 4, and the lower end of the mesh 5 is connected to a nematode adhesive membrane 6. The glass pipette 7 is fitted onto the lower part of the connecting tube 4 and is located between the rubber sleeve 2 and the connecting tube 4. The glass pipette 7 presses the edge of the nematode adhesive membrane 6 tightly.

[0013] The upper and lower parts of the connecting tube 4 are connected to a limiting protrusion. The upper part of the limiting protrusion is fixedly connected to the glass sleeve 3, and the lower part is detachably connected to the glass straw 7.

[0014] The vacuum extraction pipe 1 includes a pipe body and a piston connected inside the pipe. The upper part of the pipe body is a piston chamber, and the lower part is a transition pipe with an inner diameter smaller than that of the piston chamber. A rubber sleeve 2 is tightly fitted on the transition pipe. One end of the piston is located outside the piston chamber so as to draw and suck up liquid.

[0015] The mesh is a 60-mesh stainless steel mesh, and the nematode adhesive membrane is a 300-mesh disposable nylon film.

[0016] The method of using this invention is as follows: Pull out the upper end of the piston to directly draw up the nematode fluid. The nematodes are separated from the water through the nematode adhesive membrane 6. Then, flip up the lower end of the rubber sleeve 2 to remove the glass pipette 7, exposing the nematode adhesive membrane 6. If preparing for molecular biology experiments, the nematode adhesive membrane 6 can be washed off by stirring it in a centrifuge tube containing sterile water with tweezers. If you want to place the nematodes on a glass slide to make a temporary or permanent slide, you can use tweezers to stir the nematode adhesive membrane 6 on a slide containing sterile water or glycerin to wash off the nematodes. Example 2

[0017] The structure and connection relationship of each part of the nematode extractor described in this embodiment are the same as those in Embodiment 1. The difference is that the mesh is a 70-mesh stainless steel mesh and the nematode adhesive membrane is a 400-mesh disposable nylon film. Example 3

[0018] The structure and connection relationship of each part of the nematode extractor described in this embodiment are the same as those in Embodiment 1. The difference is that the mesh is an 80-mesh stainless steel mesh and the nematode adhesive membrane is a 500-mesh disposable nylon film.

Claims

1. A nematode sucker, characterized in that, The device includes a vacuum extraction tube, a connecting tube, and a glass suction tube connected sequentially from top to bottom. The upper part of the connecting tube is fitted with a glass sleeve, and the glass sleeve is fitted with a rubber sleeve. The upper end of the rubber sleeve is tightly fitted to the lower end of the vacuum extraction tube, so that the vacuum extraction tube and the connecting tube are fixedly connected as one unit. The lower end of the connecting tube is fitted with a mesh, and the lower end of the mesh is connected to a nematode adhesive membrane. The glass suction tube is fitted to the lower part of the connecting tube and is located between the rubber sleeve and the connecting tube.

2. The nematode extractor according to claim 1, characterized in that, The upper and lower parts of the connecting tube are connected to a limiting protrusion. The upper part of the limiting protrusion is fixedly connected to a glass sleeve, and the lower part is detachably connected to a glass straw.

3. The nematode extractor according to claim 1, characterized in that, The vacuum extraction tube includes a tube body and a piston connected inside the tube. The upper part of the tube body is a piston chamber, and the lower part is a transition tube with an inner diameter smaller than that of the piston chamber. A rubber sleeve is tightly fitted on the transition tube. One end of the piston is located outside the piston chamber to allow for the extraction of liquid.

4. A nematode sucker according to any one of claims 1 to 3, characterized in that, The mesh is a 60-80 mesh stainless steel mesh, and the nematode adhesive membrane is a 300-500 mesh disposable nylon film.

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