Protein transfer printing device with common-anode parallel structure
By using a common anode parallel structure design, the problem of uneven electric field distribution was solved, achieving efficient and high-quality transfer results for multiple samples.
Patent Information
- Application Number
- CN202423056072.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-11
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2034-12-11
AI Technical Summary
In existing protein transfer devices, the expansion of electrode width leads to uneven electric field distribution, affecting transfer efficiency and quality, especially when transferring multiple samples.
The design employs a common anode parallel structure, where multiple cathode electrodes share a single anode electrode. Combined with a circulating cooling system and a perforated design, the circulating cooling system ensures a uniform distribution of the electric field, thereby improving transfer efficiency and quality.
It achieves uniform electric field distribution during multi-sample transfer, improving transfer efficiency and quality, and avoiding the problem of poor transfer effect caused by uneven electric field.
Smart Images
Figure CN223692386U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to protein transfer technology field, concretely is a protein transfer device of common anode parallel structure. BACKGROUND
[0002] Protein transfer is the method that protein is transferred to nitrocellulose paper or pvdf membrane by electric traction, in order to more efficient transfer, often single tank equipment needs to provide transfer function for multiple samples simultaneously. However, the conventional product in the industry commonly used is that two samples are transferred simultaneously in a single tank at a time, and gradually expanded to four samples in a single tank at a time today. The expansion method is usually to widen the width between positive and negative electrodes, and directly increase two sets of sample placement positions to achieve.
[0003] But the above-mentioned method increases the electrode width, so that the electric field is reduced under the same voltage condition, causing the transfer efficiency to decrease. With the increase of the number of sample series, the electric field cannot be evenly distributed to each sample, which easily causes poor transfer effect of individual samples; therefore, we provide a protein transfer device with common anode parallel structure to solve the above problems. UTILITY MODEL CONTENT
[0004] The utility model aims at making up for the deficiency of prior art, and provides a protein transfer device with common anode parallel structure, which adopts a common anode electrode structure design, can make cathodes share one anode through parallel design, to ensure uniform distribution of electric field in the sample transfer process, thereby better improving the efficiency and quality of protein transfer, and avoiding uneven distribution of electric field leading to poor sample transfer effect and substandard quality.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a protein transfer device with common anode parallel structure, comprising a box body, a transfer slot is formed in the inside of the box body, a transfer frame is placed in the inside of the box body, a clamping groove gap is arranged at the center position in the inside of the transfer frame, and a positive electrode arrangement plate is clamped with the clamping groove gap, the inner wall of the transfer frame is used for arranging cathode circuit, and the positive electrode arrangement plate is used for arranging anode circuit; a plurality of groups of sample plates are further arranged in the inside of the transfer frame, two positive and negative electrode heads are installed on the upper surface of the transfer frame, and the two positive and negative electrode heads are respectively used for transmitting cathode and anode.
[0006] Further, the positive electrode arrangement plate is used for separating the transfer frame into two transfer cavities, the transfer cavities are used for storing sample plates, through the arrangement of transfer cavities, the sample plates in the two transfer cavities can be matched with the anode electrodes arranged on the positive electrode arrangement plate, so that the cathode electrodes arranged on the side of the transfer frame can share the anode electrodes on the positive electrode arrangement plate.
[0007] Further, the upper surface of each of the sample plates is clamped with a clamping plate, which is used to block the sample plate. By arranging the clamping plate, the sample plate can be clamped and positioned, so that the sample plate does not shake in the transfer cavity, and the distance between the sample plates does not change, so that the distance between each sample plate is equal, and the quality of the sample transfer process is uniform.
[0008] Further, the upper end of each of the positive and negative electrode heads is provided with a current connecting cylinder, which is used for connecting and transmitting the electrode. By arranging the current connecting cylinder, the anode and cathode electrodes can be connected to the positive and negative electrode heads through the current connecting cylinder, so that the anode and cathode electrodes can be arranged.
[0009] Further, the inner bottom wall of the transfer groove is fixedly connected with a sliding strip, and the outer surface of the sliding strip is slidably connected with the sample plate. By arranging the sliding strip, the sample plate can be positioned, so that the lower part of the sample plate does not shake in the transfer cavity.
[0010] Further, the outside of the box is provided with a circulating refrigeration device, the output end and the input end of the circulating refrigeration device are fixedly connected with a water outlet pipe and a water inlet pipe respectively, and the outer ends of the water outlet pipe and the water inlet pipe are connected with the input end and the output end of the box respectively. By arranging the circulating refrigeration device, the buffer solution in the transfer groove can be cooled, so that the temperature in the transfer groove is not too high during the sample transfer process, and the quality of the sample transfer is not affected.
[0011] Further, the inner wall of the box is provided with two water passing heads, and the outer ends of the water passing heads are connected with the outer ends of the water outlet pipe and the water inlet pipe respectively. The outer surface of the transfer frame is provided with a plurality of holes. By arranging the holes, the heat in the transfer frame can be quickly exchanged into the transfer groove. With the cooperation of the circulating refrigeration device, the experimental process will not generate too much heat without burying ice, which ensures the transfer efficiency and the quality of the transfer.
[0012] Compared with the prior art, the protein transfer device with the common anode parallel structure has the following beneficial effects:
[0013] 1. The common anode electrode structure design arranges the anode electrode on the positive electrode arrangement plate and the cathode electrode on the transfer frame two side partitions, forms two groups of independent and parallel transfer cavities capable of accommodating two pieces of glue, under the same voltage condition, the two groups of transfer cavities can also form the same electric field as the ordinary two-piece glue transfer frame, and the parallel design can make two cathodes share one anode, so as to ensure the uniform distribution of the electric field in the sample transfer process, thereby better improving the efficiency of protein transfer.
[0014] 2, the utility model discloses a hole's setting can make the heat in the transfer frame exchange rapidly to the frame outside, and cooperate the circulating refrigeration equipment, under the prerequisite of not needing to bury ice, to ensure that the experiment process does not produce the temperature that is too high, namely guarantee the transfer efficiency and guarantee the quality of transfer. BRIEF DESCRIPTION OF DRAWINGS
[0015] Figure 1 It is the perspective view of the whole device of the utility model;
[0016] Figure 2 It is the rear view of the whole device of the utility model;
[0017] Figure 3 It is the main view of the whole device of the utility model;
[0018] Figure 4 It is the explosion view of the whole device of the utility model;
[0019] Figure 5 It is the internal structure schematic view of the transfer frame of the utility model;
[0020] Figure 6 It is the bottom view structure schematic view of the transfer frame of the utility model;
[0021] Figure 7 It is the top view structure schematic view of the transfer frame of the utility model.
[0022] In the drawing: 1, box body; 2, transfer groove; 3, transfer frame; 4, clamping groove gap; 5, positive electrode arrangement plate; 6, transfer cavity; 7, sample plate; 8, positive and negative electrode head; 9, clamping plate; 10, current connecting cylinder; 11, slide bar; 12, circulating refrigeration equipment; 13, water outlet pipe; 14, water inlet pipe; 15, water head; 16, hole. DETAILED DESCRIPTION
[0023] The principles and features of the utility model are described below in conjunction with the drawings, and the examples are only used to explain the utility model and not to limit the scope of the utility model.
[0024] As described in the background, the traditional protein transfer reduces the electric field under the same voltage condition due to the increase of the electrode width, causing the transfer efficiency to decrease, and as the number of sample series increases, the electric field cannot be evenly distributed to each sample, which easily causes the poor effect of individual sample transfer. Therefore, the embodiment provides a protein transfer device with a common anode parallel structure, which adopts a common anode electrode structure design, can make the cathode share one anode through parallel design, to ensure the uniform distribution of electric field in the sample transfer process, thereby better improving the efficiency and quality of protein transfer, and avoiding the uneven distribution of electric field leading to poor sample transfer effect and substandard quality.
[0025] Referring to Figure 1 - Figure 7 The embodiment provides a protein transfer device with a common anode parallel structure, which comprises a box body 1, a transfer groove 2 is arranged in the box body 1, and a transfer frame 3 is arranged in the box body 1.
[0026] Referring to Figures 1 to 7 The box body 1 and the transfer groove 2 are mainly used for storing the transfer frame 3 and buffer solution, and a closing cover can be arranged on the upper portion of the box body 1 according to requirements, so that the transfer frame 3 in the transfer groove 2 can be sealed.
[0027] The inner bottom wall of the transfer groove 2 is fixedly connected with a sliding strip 11, and the outer surface of the sliding strip 11 is slidably connected with a sample plate 7; the sliding strip 11 can be used for limiting the sample plate 7, so that the lower portion of the sample plate 7 cannot shake in the transfer cavity 6, and the sample plate 7 can be better placed in the transfer cavity 6, thereby greatly improving the stability of the sample plate 7 in the transfer cavity 6 and the convenience of adjusting the position of the sample plate 7.
[0028] A circulating refrigeration device 12 is arranged outside the box body 1, an outlet pipe 13 and an inlet pipe 14 are fixedly connected to the output end and the input end of the circulating refrigeration device 12, respectively, and the outer ends of the outlet pipe 13 and the inlet pipe 14 are connected to the input end and the output end of the box body 1, respectively.
[0029] The circulating refrigeration device 12 can be used for cooling the buffer solution in the transfer groove 2, so that the high temperature in the transfer groove 2 does not affect the quality of sample transfer in the sample transfer process, and the practicability of the whole device and the quality of sample transfer are greatly improved.
[0030] Two water passing heads 15 are mounted on the inner wall of the box body 1, the two water passing heads 15 are connected to the outer ends of the outlet pipe 13 and the inlet pipe 14, respectively, and a plurality of holes 16 are arranged on the outer surface of the transfer frame 3.
[0031] The water passing heads 15 can be used for better conveying the buffer solution, the holes 16 can be used for rapidly exchanging heat in the transfer frame 3 to the transfer groove 2, and the circulating refrigeration device 12 can be used for guaranteeing that the experimental process does not generate high heat without burying ice, that is, the transfer efficiency and the transfer quality are guaranteed.
[0032] A clamping groove gap 4 is arranged at the center position in the transfer frame 3, and a positive electrode arrangement plate 5 is clamped to the clamping groove gap 4, the inner wall of the transfer frame 3 is used for arranging a cathode circuit, and the positive electrode arrangement plate 5 is used for arranging an anode circuit.
[0033] Referring to Figures 1 to 7The card slot gap 4 is mainly used for clamping and installing the positive arrangement plate 5, so that the positive arrangement plate 5 is located at the center position of the transfer frame 3, and the positive arrangement plate 5 is used for arranging the anode electrode line, and the cathode anode line is arranged on the inner wall of the transfer frame 3 on both sides of the anode electrode line, so that two cathode electrodes share one anode electrode, so as to ensure the uniformity of the electric field distribution in the sample transfer process.
[0034] The positive arrangement plate 5 is used for separating the transfer frame 3 into two transfer cavities 6 for storing the sample plate 7. Through the arrangement of the transfer cavity 6, the sample plate 7 in the two transfer cavities 6 can cooperate with the anode electrode arranged on the positive arrangement plate 5, so that the cathode electrode arranged on the side of the transfer frame 3 can share the anode electrode on the positive arrangement plate 5, thereby realizing the parallel structure design, so as to realize the uniform distribution of the electric field in the sample transfer process, and avoid the uneven distribution of the electric field caused by the traditional series accumulation type, which affects the efficiency and quality of the sample transfer.
[0035] The inside of the transfer frame 3 is also provided with a plurality of groups of sample plates 7, and the upper surface of the transfer frame 3 is provided with two positive and negative electrode heads 8, which are respectively used for the transmission of the cathode and the anode.
[0036] Reference Figures 1 to 7 The arrangement of the two positive and negative electrode heads 8 can facilitate the connection of the anode and the cathode respectively, so as to arrange the cathode and the anode on the positive arrangement plate 5 and the transfer frame 3, and facilitate the transmission of direct current through the positive and negative electrode heads 8.
[0037] The upper surface of each group of sample plates 7 is clamped with a clamping plate 9, which is used for blocking the sample plate 7. Through the arrangement of the clamping plate 9, the sample plate 7 can be clamped and limited, so as to avoid the shaking of the sample plate 7 in the transfer cavity 6 and the change of the distance between the sample plates 7, so as to ensure that the distance between each sample plate 7 is equal, and avoid the uneven quality in the sample transfer process.
[0038] In addition, the arrangement of the clamping plate 9 can better set the distance between the sample plates 7, so as to obtain a narrower electrode distance, a higher electric field strength under the same voltage, and a higher transfer efficiency.
[0039] The upper end of the two positive and negative electrode heads 8 is provided with a current connecting cylinder 10, which is used for connecting and transmitting the electrode. Through the arrangement of the current connecting cylinder 10, the anode and the cathode electrode can be better connected with the positive and negative electrode heads 8 through the current connecting cylinder 10, so as to arrange the anode electrode and the cathode electrode.
[0040] In addition, the positive and negative electrode heads 8 can be connected with the direct current power supply through the current connecting cylinder 10, so as to transfer the sample to the film under the driving of the power supply.
[0041] Working principle: when the sample needs to be transferred, first place the sample plate 7 containing the sample in the transfer cavity 6, after the sample plate 7 is placed, use the clamp plate 9 to clamp several sample plates 7, at this time, the current connecting cylinder 10 can be connected with the direct current power supply, the current connecting cylinder 10 transmits positive and negative current through the positive and negative electrode heads 8, the positive current is transmitted to the anode electrode circuit arranged on the surface of the positive electrode arrangement plate 5, and the negative current is transmitted to the cathode electrode circuit arranged on the inner wall of the transfer frame 3, so that two cathode electrodes share one anode electrode, and then through the parallel design, compared with the series design, the electric field intensity is higher under the same voltage, the transfer efficiency is higher, and in the transfer process, the circulating refrigeration equipment 12 is started, the circulating refrigeration equipment 12 can cool the buffer solution in the transfer tank 2 through the water outlet pipe 13 and the water inlet pipe 14, and through the setting of the hole 16, the experimental process will not produce too much heat without burying ice, that is, the transfer efficiency is guaranteed and the transfer quality is guaranteed.
Claims
1. A protein transfer device of a common anode parallel structure comprising a box (1), characterized in that: The inside of the box (1) is provided with a transfer slot (2), the inside of the box (1) is placed with a transfer frame (3), the inside of the transfer frame (3) is provided with a clamping slot gap (4) at the center position, and a positive electrode arrangement plate (5) is connected with the clamping slot gap (4), the inner wall of the transfer frame (3) is used for the arrangement of the cathode circuit, and the positive electrode arrangement plate (5) is used for the arrangement of the anode circuit; the inside of the transfer frame (3) is also provided with a plurality of groups of sample plates (7), the upper surface of the transfer frame (3) is provided with two positive and negative electrode heads (8), and the two positive and negative electrode heads (8) are used for the transmission of positive and negative electrodes respectively.
2. The protein transfer apparatus of claim 1, wherein: The positive electrode arrangement plate (5) is used for separating the transfer frame (3) into two transfer cavities (6), and the transfer cavities (6) are used for storing the sample plates (7).
3. The protein transfer apparatus of claim 1, wherein: The upper surface of each group of sample plates (7) is connected with a clamping plate (9), and the clamping plate (9) is used for blocking the sample plates (7).
4. The protein transfer apparatus of claim 1, wherein: The upper end of the two positive and negative electrode heads (8) is provided with a current connecting cylinder (10), and the current connecting cylinder (10) is used for connecting and transmitting the electrode.
5. The protein transfer apparatus of claim 1, wherein: The inner bottom wall of the transfer slot (2) is fixedly connected with a sliding bar (11), and the outer surface of the sliding bar (11) is slidably connected with the sample plate (7).
6. The protein transfer apparatus of claim 1, wherein: The outside of the box (1) is provided with a circulating refrigeration device (12), and the output end and the input end of the circulating refrigeration device (12) are fixedly connected with a water outlet pipe (13) and a water inlet pipe (14) respectively, and the outer ends of the water outlet pipe (13) and the water inlet pipe (14) are connected with the input end and the output end of the box (1) respectively.
7. The protein transfer apparatus of claim 6, wherein: The inner wall of the box (1) is provided with two water passing heads (15), and the outer ends of the water passing heads (15) are connected with the outer ends of the water outlet pipe (13) and the water inlet pipe (14) respectively, and the outer surface of the transfer frame (3) is provided with a plurality of holes (16).