Separation and preparation device of scyllo-inositol

Abstract

The utility model discloses a separation and preparation device of scyllo-inositol, and relates to the technical field of scyllo-inositol production, inositol-containing dehydrogenase and whole-cell wet thalli containing scyllo-inositol dehydrogenase are added into a myo-inositol solution, transformation is carried out in a PBS buffer solution, the obtained transformation solution enters a ceramic membrane device, and the ceramic membrane device is used for separating and preparing scyllo-inositol. Ceramic membrane clear liquid enters a concentration tank to be concentrated until crystals are separated out, ceramic membrane concentrated liquid is subjected to environment-friendly pollution discharge treatment, concentrated materials enter a first centrifugal machine to be centrifugally filtered, a filter cake is a mixed crude product of scyllo-inositol and inositol, the mixed crude product and purified water are beaten and washed in a beating tank, scyllo-inositol is very low in solubility and slightly soluble in water, and the scyllo-inositol is separated out. The scyllo-inositol is high in solubility, the aim of separation is achieved through water washing, finally separation of scyllo-inositol and myo-inositol is achieved through the second centrifugal machine, boric acid, strong acid, strong alkali and organic solvents are not used in the separation preparation process, the environment is not polluted, operation is safe, and the recovery cost is low.

Description

Technical Field

[0001] This utility model relates to the field of squalene production technology, specifically to a squalene separation and preparation apparatus. Background Technology

[0002] Existing squalene production processes use muscle inositol as a raw material, first converting it to squalene monoketone, then mostly converting the latter back to squalene, but leaving a significant portion of unreacted squalene monoketone, resulting in a mixture. Additionally, a significant amount of the byproduct squalene is formed during this conversion. To separate the desired squalene from the mixture of unreacted squalene monoketone and the byproduct squalene, squalene is first chemically converted to a squalene-diborate-disodium complex, followed by hydrolysis with hydrochloric acid in a mixture of methane and water. This conversion requires the use of boric acid to form the squalene-diborate-disodium complex salt, and the use of concentrated hydrochloric acid is not only corrosive to equipment but also requires special operating procedures to ensure safety and avoidance of harmful gases. Furthermore, because this method requires the use of boric acid to chemically selectively derivatize squalene into the squalene-diborate-disodium complex salt during the recovery of squalene, and boric acid is a toxic substance, it is crucial to remove boric acid from the material to an appropriate level in subsequent steps. The process is cumbersome, costly, and highly polluting. Summary of the Invention

[0003] The technical problem to be solved by this utility model is to provide a squalene separation and preparation device that addresses the shortcomings of the existing technology. The device is simple to use, has low production cost, and is environmentally friendly.

[0004] To solve the above-mentioned technical problems, the technical solution of this utility model is as follows:

[0005] A device for separating and preparing squalinositol includes a conversion tank, the inlet of which is connected to a muscle inositol solution tank, a whole-cell wet bacterial cell tank and a PBS buffer tank via pipes, the outlet of which is connected to a conversion liquid tank via pipes, the outlet of which is connected to a ceramic membrane device via pipes, and the clear liquid outlet of which is connected to a concentration tank via pipes.

[0006] The outlet of the concentration tank is connected to a first centrifuge via a pipeline. The solid phase outlet of the first centrifuge is connected to a pulping tank. The inlet of the pulping tank is connected to a purified water tank. The outlet of the pulping tank is connected to a second centrifuge. The solid phase outlet of the second centrifuge is connected to a squalene tank.

[0007] As an improved technical solution, the clear liquid outlet of the ceramic membrane device is connected to an ultrafiltration membrane device via a pipeline, and the clear liquid outlet of the ultrafiltration membrane device is connected to the concentration tank via a pipeline.

[0008] As an improved technical solution, the clarified liquid outlet of the ultrafiltration membrane device is connected to a decolorization tank via a pipeline, the inlet of the decolorization tank is connected to an activated carbon tank, the outlet of the decolorization tank is connected to a filter, the outlet of the filter is connected to a decolorization liquid tank, and the outlet of the decolorization liquid tank is connected to the concentration tank.

[0009] As an improved technical solution, the liquid phase outlet of the first centrifuge is connected to a collection tank.

[0010] As an improved technical solution, the liquid phase outlet of the second centrifuge is connected to the collection tank.

[0011] As an improved technical solution, the outlet of the collection tank is connected to the conversion tank via a pipeline.

[0012] As a preferred technical solution, the solid phase outlet of the second centrifuge is connected to a dryer, and the outlet of the dryer is connected to the squalene tank.

[0013] As a preferred technical solution, the pore size of the ceramic membrane device is 20-100 nm.

[0014] As a preferred technical solution, the pore size of the ultrafiltration membrane device is 1000-10000 Da.

[0015] Due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0016] This invention discloses a device for separating and preparing squalene, comprising a conversion tank. The inlet of the conversion tank is connected via pipes to a muscle inositol solution tank, a whole-cell wet bacterial cell tank, and a PBS buffer tank. The outlet of the conversion tank is connected via pipes to a conversion liquid tank. The outlet of the conversion liquid tank is connected via pipes to a ceramic membrane device. The clear liquid outlet of the ceramic membrane device is connected via pipes to a concentration tank. The outlet of the concentration tank is connected via pipes to a first centrifuge. The solid phase outlet of the first centrifuge is connected to a pulping tank. The inlet of the pulping tank is connected to a purified water tank. The outlet of the pulping tank is connected to a second centrifuge. The solid phase outlet of the second centrifuge is connected to a squalene tank. Whole-cell wet bacteria containing inositol dehydrogenase and squalinositol dehydrogenase were added to the muscle inositol solution and transformed in PBS buffer. The resulting transformed solution entered a ceramic membrane device. The clear solution from the ceramic membrane was concentrated in a concentration tank until crystals precipitated. The concentrated solution from the ceramic membrane was then treated for environmental pollution discharge. The concentrated material entered a first centrifuge for centrifugation and filtration. The filter cake was a crude mixture of squalinositol and inositol. The crude mixture and purified water were then pulped and washed in a pulping tank. Squalinositol has very low solubility and is slightly soluble in water, while muscle inositol has high solubility. Separation was achieved by washing with water. Finally, a second centrifuge was used to separate squalinositol and muscle inositol. The separation and preparation process does not use boric acid, strong acids or bases, or organic solvents, so it does not pollute the environment, is safe to operate, and has low recovery costs.

[0017] The clarified liquid outlet of the ceramic membrane device of this invention is connected to an ultrafiltration membrane device via a pipeline, and the clarified liquid outlet of the ultrafiltration membrane device is connected to the concentration tank via a pipeline. The pore size of the ceramic membrane device is 20-100 nm. The ultrafiltration membrane device further purifies the material, removing large molecular impurities and improving the purity of the product.

[0018] The clarified liquid outlet of the ultrafiltration membrane device is connected to a decolorization tank via a pipeline. The inlet of the decolorization tank is connected to an activated carbon tank. The outlet of the decolorization tank is connected to a filter. The outlet of the filter is connected to a decolorized liquid tank. The outlet of the decolorized liquid tank is connected to a concentration tank. The pore size of the ultrafiltration membrane device is 1000-10000 Da. The decolorization tank can decolorize the product using activated carbon, thus improving the product quality.

[0019] The liquid phase outlet of the first centrifuge is connected to a collection tank, and the liquid phase outlet of the second centrifuge is also connected to the collection tank. The outlet of the collection tank is connected to the conversion tank via a pipeline. The concentrated filtrate and the pulped filtrate are mixed and then re-enter the conversion tank for conversion. The materials are recycled, eliminating the need for a complex recovery system and thus reducing costs.

[0020] The solid phase outlet of the second centrifuge is connected to a dryer, and the outlet of the dryer is connected to the squalene tank. By further drying the squalene material, the purity and quality of the squalene product are improved. Attached Figure Description

[0021] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0022] Figure 1 This is a structural schematic diagram of an embodiment of the present utility model;

[0023] The components include: 1. Conversion tank; 2. Muscle inositol solution tank; 3. Whole-cell wet bacterial cell tank; 4. PBS buffer tank; 5. Conversion solution tank; 6. Ceramic membrane device; 7. Concentration tank; 8. First centrifuge; 9. Pulping tank; 10. Purified water tank; 11. Second centrifuge; 12. Squalene tank; 13. Ultrafiltration membrane device; 14. Decolorization tank; 15. Activated carbon tank; 16. Filter; 17. Decolorization solution tank; 18. Collection tank; 19. Desiccant. Detailed Implementation

[0024] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0025] like Figure 1As shown, an apparatus for the separation and preparation of squalene includes a conversion tank 1. The inlet of the conversion tank 1 is connected to a muscle inositol solution tank 2, a whole-cell wet bacterial cell tank 3, and a PBS buffer tank 4 via pipes. The outlet of the conversion tank 1 is connected to a conversion liquid tank 5 via pipes. The outlet of the conversion liquid tank 5 is connected to a ceramic membrane device 6 via pipes. The clear liquid outlet of the ceramic membrane device 6 is connected to a concentration tank 7 via pipes. The outlet of the concentration tank 7 is connected to a first centrifuge 8 via pipes. The solid phase outlet of the first centrifuge 8 is connected to a pulping tank 9. The inlet of the pulping tank 9 is connected to a purified water tank 10. The outlet of the pulping tank 9 is connected to a second centrifuge 11. The solid phase outlet of the second centrifuge 11 is connected to a squalene tank 12. Whole-cell wet bacteria containing inositol dehydrogenase and squalinositol dehydrogenase are added to the muscle inositol solution and transformed in PBS buffer. The resulting transformed solution enters ceramic membrane device 6. The clear solution from the ceramic membrane enters concentration tank 7 for concentration until crystals precipitate. The concentrated solution from the ceramic membrane is then treated for environmental pollution discharge. The concentrated material enters the first centrifuge 8 for centrifugation and filtration. The filter cake is a crude mixture of squalinositol and inositol. The crude mixture and purified water are pulped and washed in pulping tank 9. Squalinositol has very low solubility and is slightly soluble in water, while muscle inositol has high solubility. The separation is achieved by washing with water. Finally, the squalinositol and muscle inositol are separated by a second centrifuge 11. The separation and preparation process does not use boric acid, strong acids or bases, or organic solvents, so it does not pollute the environment, is safe to operate, and has low recovery costs.

[0026] The clarified liquid outlet of the ceramic membrane device 6 is connected to the ultrafiltration membrane device 13 via a pipeline, and the clarified liquid outlet of the ultrafiltration membrane device 13 is connected to the concentration tank 7 via a pipeline. The pore size of the ceramic membrane device 6 is 20-100 nm. The material is further purified by the ultrafiltration membrane device 13, removing large molecular impurities and improving the purity of the product.

[0027] The clarified liquid outlet of the ultrafiltration membrane device 13 is connected to a decolorization tank 14 via a pipeline. The inlet of the decolorization tank 14 is connected to an activated carbon tank 15. The outlet of the decolorization tank 14 is connected to a filter 16. The outlet of the filter 16 is connected to a decolorization liquid tank 17. The outlet of the decolorization liquid tank 17 is connected to the concentration tank 7. The pore size of the ultrafiltration membrane device 13 is 1000-10000 Da. The decolorization tank 14 can decolorize the product using activated carbon, thereby improving the product quality.

[0028] The liquid phase outlet of the first centrifuge 8 is connected to a collection tank 18, and the liquid phase outlet of the second centrifuge 11 is also connected to the collection tank 18. The outlet of the collection tank 18 is connected to the conversion tank 1 via a pipeline. The concentrated filtrate and the pulped filtrate are mixed and then re-enter the conversion tank 1 for conversion. The materials are recycled, eliminating the need for a complex recovery system and thus reducing costs.

[0029] The solid phase outlet of the second centrifuge 11 is connected to a dryer 19, and the outlet of the dryer 19 is connected to the squalene tank 12. By further drying the squalene material, the purity and quality of the squalene product are improved.

[0030] It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that after reading the teachings of this invention, those skilled in the art can make various alterations or modifications to the invention, and these equivalent forms also fall within the scope defined by the appended claims.

Claims

1. A device for the isolation of inositol comprising a conversion tank, characterized in that: The inlet of the conversion tank is communicated with a muscle inositol solution tank, a whole cell wet bacteria tank and a PBS buffer tank respectively through pipelines, the outlet of the conversion tank is communicated with a conversion liquid tank through a pipeline, the outlet of the conversion liquid tank is communicated with a ceramic membrane device through a pipeline, and the clear liquid outlet of the ceramic membrane device is communicated with a concentration tank through a pipeline. The outlet of the concentration tank is communicated with a first centrifuge through a pipeline, the solid phase outlet of the first centrifuge is communicated with a beating tank, the inlet of the beating tank is communicated with a purified water tank, the outlet of the beating tank is communicated with a second centrifuge, and the solid phase outlet of the second centrifuge is communicated with a squalene inositol tank.

2. A device for the isolation of inositol as claimed in claim 1, characterized in that: The clear liquid outlet of the ceramic membrane device is communicated with an ultrafiltration membrane device through a pipeline, and the clear liquid outlet of the ultrafiltration membrane device is communicated with the concentration tank through a pipeline.

3. A device for the isolation of scyllitol according to claim 2, characterized in that: The clear liquid outlet of the ultrafiltration membrane device is communicated with a decolorizing tank through a pipeline, the inlet of the decolorizing tank is communicated with an activated carbon tank, the outlet of the decolorizing tank is communicated with a filter, the outlet of the filter is communicated with a decolorizing liquid tank, and the outlet of the decolorizing liquid tank is communicated with the concentration tank.

4. The apparatus for separating and preparing scyllitol according to claim 1, wherein: The liquid phase outlet of the first centrifuge is communicated with a collection tank.

5. A device for the isolation of scyllitol according to claim 4, characterized in that: The liquid phase outlet of the second centrifuge is communicated with the collection tank.

6. A device for the isolation of scyllitol according to claim 5, characterized in that: The outlet of the collection tank is communicated with the conversion tank through a pipeline.

7. The apparatus for separating and preparing scyllite according to claim 1, wherein: The solid phase outlet of the second centrifuge is communicated with a dryer, and the outlet of the dryer is communicated with the squalene inositol tank.

8. The apparatus for separating and preparing scyllite according to claim 1, wherein: The pore size of the ceramic membrane device is 20-100nm.

9. The apparatus for separating and preparing scyllite according to claim 2, wherein: The pore size of the ultrafiltration membrane device is 1000-10000Da.

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