Separating pipe for centrifugal machine
By installing a grinding baffle ring and a grinding liner inside the centrifuge's separation tube, the tissue sample is broken up by the collision of grinding balls. This solves the problem of needing to add grinding media in the prior art, simplifies the operation process, and improves the breaking efficiency.
Patent Information
- Application Number
- CN202422877577.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-25
- Publication Date
- 2026-01-13
- Estimated Expiration
- 2034-11-25
AI Technical Summary
Existing centrifuge tubes require the addition of grinding media to assist in tissue disruption during liquid nitrogen grinding, and the media needs to be screened out after centrifugation, increasing the complexity of the operation.
A grinding retaining ring and a grinding liner are installed inside the centrifuge's separation tube. Grinding balls are evenly distributed on the inner wall. The tissue sample is broken up by the collision during high-speed rotation, eliminating the need to add grinding media.
It simplifies the operation steps, improves the efficiency of tissue sample crushing, avoids the step of screening grinding media, and makes the operation more convenient.
Smart Images
Figure CN223788642U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of centrifugal crushing technology, specifically to a centrifuge separation tube. Background Technology
[0002] In the process of extracting tissue components by grinding with liquid nitrogen, the tissue sample needs to be aliquoted into appropriate centrifuge tubes, grinding media is added to the centrifuge tubes, the caps of the centrifuge tubes are tightened and inserted into the cryo-adaptor, and a special adapter clamp is used to place the entire cryo-adaptor into liquid nitrogen for cooling. After cooling, the entire cryo-adaptor is removed and placed in a tissue grinder for centrifugation. After centrifugation, the separation tube is removed.
[0003] The prior art discloses a centrifuge tube, which includes a tube body and a tube cap connected to the tube body. The tube body has a valve baffle that is recessed into the inner cavity of the tube body at the tube opening. The tube cap is hinged to the edge of the tube opening of the tube body. This not only solves the problem of sample splashing inside the tube, but also makes it more convenient for experimental personnel to operate, improves the overall experimental efficiency, and saves samples and consumables.
[0004] While the centrifuge tubes in the aforementioned prior art can solve the problem of sample splashing, when using these centrifuge tubes for liquid nitrogen grinding, it is still necessary to add grinding media into the tube to assist in tissue disruption. After centrifugation, the grinding media needs to be sieved out from the tissue fragments, which increases the complexity of the actual operation and causes inconvenience. Utility Model Content
[0005] Therefore, this application provides a centrifuge separation tube to solve the problem in the prior art where grinding media are added to the tube to assist in tissue fragmentation, and after centrifugation, the grinding media needs to be screened out from the tissue fragments, which increases the cumbersomeness of actual operation.
[0006] To achieve the above objectives, this application provides the following technical solution:
[0007] A centrifuge separation tube is mainly used for centrifugation and crushing of tissue samples during liquid nitrogen grinding. Specifically, it includes a tube body and a tube cap installed at the upper opening of the tube body. The inner cavity of the tube body is fitted with grinding baffle rings and grinding inner liner distributed vertically. The inner wall of the lower opening of the grinding baffle ring has an inclined surface, which is opposite to the inner wall of the grinding inner liner. Grinding balls are evenly distributed on both the inclined surface and the inner wall of the grinding inner liner.
[0008] Optionally, the grinding retaining ring is a circular ring adapted to the inner diameter of the tube, and the lower end opening of the circular ring is funnel-shaped, with the top of the inclined surface of the funnel facing the center of the circular ring.
[0009] Optionally, the grinding retaining ring is slidably fitted inside the upper end of the tube body, the lower end of the grinding retaining ring presses against the upper end face of the grinding inner liner, and the upper end of the grinding retaining ring abuts against the tube cap.
[0010] Optionally, the grinding liner is a sleeve-shaped tube with an open top, and the grinding liner is slidably fitted and adapted to the lower end of the inner cavity of the tube.
[0011] Optionally, the outer wall of the tube has a label area, and a label nameplate is detachably provided in the label area.
[0012] Optionally, the grinding retaining ring, grinding liner, and grinding ball are all made of stainless steel.
[0013] Alternatively, the pipe cap is threaded onto the upper exterior of the pipe body, and a sealing plug is installed at the bottom of the inner cavity of the pipe cap. The sealing plug is fitted into the upper opening of the pipe body and abuts against the upper end of the grinding retaining ring.
[0014] Further optionally, the lower end of the sealing plug is frustum-shaped, and the area of the lower base of the frustum is smaller than the area of the upper base, and the upper opening of the grinding retaining ring is funnel-shaped and adapted to the lower end of the sealing plug.
[0015] Alternatively, the bottom end of the sealing plug is fitted with a grinding chain coaxially aligned with the tube body, and the grinding chain is fitted with evenly distributed grinding balls.
[0016] Compared with the prior art, this application has at least the following beneficial effects:
[0017] By installing grinding baffles and grinding liner at the upper and lower ends of the tube's inner cavity, respectively, and utilizing the inclined surface of the grinding baffles and the evenly distributed grinding balls on the inner wall of the grinding liner, the tissue sample inside the tube will collide at high speed with the surrounding grinding balls during centrifugation, thereby achieving crushing centrifugation. There is no need to add grinding media, saving the step of screening grinding media, optimizing the actual operation steps, making the operation simple and convenient, and bringing convenience to actual centrifugation operations. Attached Figure Description
[0018] To more intuitively illustrate the prior art and this application, exemplary drawings are provided below. It should be understood that the specific shapes and structures shown in the drawings should not generally be regarded as limiting conditions for implementing this application; for example, based on the technical concept disclosed in this application and the exemplary drawings, those skilled in the art are able to easily make conventional adjustments or further optimizations to the addition / reduction / classification, specific shapes, positional relationships, connection methods, size ratios, etc. of certain units (components).
[0019] Figure 1 This is a schematic diagram of the structure of a centrifuge separation tube provided in one embodiment of this application;
[0020] Figure 2 This is a front cross-sectional view of a centrifuge separation tube provided in one embodiment of this application.
[0021] Explanation of reference numerals in the attached figures:
[0022] 1. Tube body; 2. Tube cap; 3. Grinding retaining ring; 4. Grinding inner liner; 5. Grinding ball; 6. Sealing plug; 7. Grinding chain; 8. Label area. Detailed Implementation
[0023] The present application will be further described in detail below with reference to the accompanying drawings and specific embodiments.
[0024] like Figure 1 and Figure 2 As shown, a centrifuge separation tube is mainly used for centrifugation and crushing of tissue samples during liquid nitrogen grinding. Specifically, it includes a tube body 1 and a tube cap 2 installed at the upper opening of the tube body 1. The inner cavity of the tube body 1 is fitted with grinding baffle rings 3 and grinding inner liner 4 distributed vertically. The inner wall of the lower opening of the grinding baffle ring 3 has an inclined surface, which is opposite to the inner wall of the grinding inner liner 4. The inclined surface and the inner wall of the grinding inner liner 4 are both equipped with uniformly distributed grinding balls 5. When the tube body 1 rotates under the action of the centrifuge, the tissue sample in the tube body 1 will rotate accordingly and collide with the inner wall of the grinding inner liner 4 and the inclined surface of the grinding baffle ring 3. The uniform distribution of the grinding balls 5 can improve the actual collision effect, thereby replacing the external grinding medium and achieving high-speed crushing.
[0025] By installing a grinding baffle ring 3 and a grinding inner liner 4 at the upper and lower ends of the inner cavity of the tube body 1 respectively, and utilizing the inclined surface of the grinding baffle ring 3 and the grinding balls 5 evenly distributed on the inner wall of the grinding inner liner 4, the tissue sample inside the tube body 1 will collide at high speed with the surrounding grinding balls 5 during centrifugation, thereby achieving crushing centrifugation. There is no need to add grinding media, saving the step of screening grinding media, optimizing the actual operation steps, making the operation simple and convenient, and bringing convenience to actual centrifugation operations.
[0026] Specifically, such as Figure 2 As shown, the grinding baffle ring 3 is a circular ring that matches the inner diameter of the tube body 1, and the lower opening of the circular ring is funnel-shaped, with the top of the inclined surface of the funnel facing the center of the circular ring. The longitudinal section of the lower opening of the grinding baffle ring 3 is "eight"-shaped, which can increase the collision force of the tissue sample, thereby improving the actual centrifugation effect.
[0027] Specifically, such as Figure 2As shown, the grinding retaining ring 3 is slidably fitted inside the upper end of the tube body 1, and the lower end of the grinding retaining ring 3 presses against the upper end face of the grinding inner liner 4. The upper end of the grinding retaining ring 3 abuts against the tube cap 2. The grinding inner liner 4 is a sleeve-shaped tube with an open upper end. The grinding inner liner 4 and the lower end of the inner cavity of the tube body 1 are slidably fitted and mutually adapted to each other, which facilitates the disassembly and cleaning of the grinding retaining ring 3 and the grinding inner liner 4. After complete assembly, the grinding retaining ring 3 is pressed against the tube cap 2, so that the grinding retaining ring 3 and the grinding inner liner 4 can be stably installed inside the tube body 1.
[0028] Specifically, such as Figure 1 As shown, the outer wall of the tube body 1 has a label area 8, and a label nameplate is detachably installed in the label area 8. The label nameplate can be installed by plugging in. By installing the label nameplate, the name and type of tissue sample inside the tube body 1 can be distinguished, which brings convenience to actual experiments.
[0029] Specifically, the grinding retaining ring 3, the grinding inner liner 4, and the grinding ball 5 are all made of stainless steel, which has good corrosion resistance and wear resistance, so as to centrifuge and crush biological tissue samples.
[0030] Furthermore, such as Figure 2 As shown, the tube cap 2 is threaded onto the upper exterior of the tube body 1. A sealing plug 6 is installed at the bottom of the inner cavity of the tube cap 2. The sealing plug 6 is fitted into the upper opening of the tube body 1 and abuts against the upper end of the grinding retaining ring 3. The lower end of the sealing plug 6 is frustum-shaped, and the area of the lower base of the frustum is smaller than the area of the upper base. The upper opening of the grinding retaining ring 3 is funnel-shaped and matches the lower end of the sealing plug 6. The upper opening of the grinding retaining ring 3 is sealed by the sealing plug 6 to improve the sealing performance and prevent the tissue sample in the tube body 1 from splashing during high-speed centrifugation.
[0031] Furthermore, such as Figure 2 As shown, a grinding chain 7 is installed at the bottom of the sealing plug 6 and is coaxially aligned with the tube body 1. The grinding chain 7 is equipped with evenly distributed grinding balls 5. On the one hand, the grinding chain 7 is opposite to the inclined surface of the grinding baffle ring 3 and the inner wall of the grinding inner liner 4, thereby increasing the contact area between the tissue sample and the grinding balls 5 during centrifugation, thus improving the actual centrifugation and crushing effect. On the other hand, during the rotational centrifugation process, the grinding chain 7 will swing inside the tube body 1, thereby increasing the crushing force on the tissue sample, thus improving the crushing effect of the tissue sample.
[0032] The technical features of the above embodiments can be combined in any way as long as there is no contradiction in the combination of these technical features. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described; these embodiments not explicitly written should also be considered to be within the scope of this specification.
Claims
1. A centrifuge separator tube, comprising a tube body (1) and a tube cap (2) installed at the upper opening of the tube body (1), characterized in that, The inner cavity of the tube (1) is fitted with a grinding baffle ring (3) and a grinding inner liner (4) distributed vertically. The inner wall of the lower opening of the grinding baffle ring (3) has a slope, which is opposite to the inner wall of the grinding inner liner (4). Grinding balls (5) are evenly distributed on both the slope and the inner wall of the grinding inner liner (4).
2. A centrifuge separation tube according to claim 1, characterized in that, The grinding retaining ring (3) is a circular ring that is adapted to the inner diameter of the tube body (1), and the lower end of the circular ring is funnel-shaped, with the top of the inclined surface of the funnel facing the center of the circular ring.
3. A centrifuge separation tube according to claim 1, characterized in that, The grinding retaining ring (3) is slidably fitted inside the upper end of the tube body (1), the lower end of the grinding retaining ring (3) presses against the upper end face of the grinding inner liner (4), and the upper end of the grinding retaining ring (3) abuts against the tube cover (2).
4. A centrifuge separation tube according to claim 3, characterized in that, The pipe cap (2) is threaded onto the upper outside of the pipe body (1). A sealing plug (6) is installed at the bottom of the inner cavity of the pipe cap (2). The sealing plug (6) is fitted into the upper opening of the pipe body (1) and abuts against the upper end of the grinding retaining ring (3).
5. A centrifuge separation tube according to claim 4, characterized in that, The lower end of the sealing plug (6) is frustum-shaped, and the area of the lower base of the frustum is smaller than the area of the upper base. The upper opening of the grinding retaining ring (3) is funnel-shaped and is adapted to the lower end of the sealing plug (6).
6. A centrifuge separation tube according to claim 4, characterized in that, The bottom end of the sealing plug (6) is equipped with a grinding chain (7) that is coaxially aligned with the tube body (1), and the grinding chain (7) is equipped with evenly distributed grinding balls (5).
7. A centrifuge separation tube according to claim 1, characterized in that, The grinding liner (4) is a sleeve-shaped tube with an open top. The grinding liner (4) is slidably fitted onto the lower end of the inner cavity of the tube body (1) and is adapted to each other.
8. A centrifuge separation tube according to claim 1, characterized in that, The outer wall of the tube (1) has a label area (8), and a label nameplate is detachably provided in the label area (8).
9. A centrifuge separation tube according to any one of claims 1-8, characterized in that, The grinding retaining ring (3), grinding inner liner (4), and grinding ball (5) are all made of stainless steel.