Culture device for bacillus cereus in Maotai-flavor yeast for making hard liquor

Abstract

The utility model relates to the technical field of liquor fermentation, and discloses a culture device for bacillus cereus in Maotai-flavor yeast for making hard liquor, which comprises a tank body, a sampling tube is arranged at the bottom of the tank body, a sample storage barrel is fixed at the discharge end of the sampling tube and is communicated with the sampling tube, a partition plate for dividing an inner cavity into a cavity A and a cavity B is fixed in the sample storage barrel, and the sampling tube is communicated with the partition plate. An annular groove is formed in the position, away from the sampling pipe, of the inner wall of the sample storage barrel, a check ring is installed in the annular groove, a rotatable rubber plug is installed between the check ring and the partition plate, the rubber plug is tightly pressed on the partition plate through the check ring, a through hole B is formed in the rubber plug, and the through hole A is located on the rotating track of the through hole B; a square hole is further formed in the center of the rubber plug. The sampling device has the beneficial effects that a test tube is inserted into the through hole B for sampling, so that a culture solution is prevented from remaining in the through hole B, and the accuracy of a sampling detection result is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to liquor fermentation technical field especially a culture device of bacillus cereus in sauce type Daqu. BACKGROUND

[0002] Bacillus belongs to bacillus family, bacillus genus, is a kind of gram-positive bacteria that can produce endospore resistance, cell is rod-shaped and outer layer is covered with a large number of calcium pyridine dicarboxylate. Bacillus has thick and low water content multilayer structure, so it has strong refractive property, good stability, oxidation resistance, extrusion resistance, high temperature resistance, can tolerate 60 DEG C high temperature for a long time, can survive 20 min at temperature 120 DEG C, and acid and alkali resistant, can maintain activity in gastric acid environment, and currently more applied have bacillus subtilis, bacillus licheniformis, bacillus cereus and other types.

[0003] In the culture process of bacillus, generally need to stir and aerate the culture fermentation broth, improve culture effect, adjust the aeration amount with the change of fermentation time, and also need to add culture medium and other fermentation substances in time during fermentation.

[0004] Chinese utility model patent (authorization announcement number: CN217103837U name: a high-efficiency culture device for bacillus) includes culture barrel, four first air pipes are symmetrically connected on the ring pipe outside the upper end of the culture barrel, the inner end of the first air pipe is inserted into the culture barrel and connected with the upper end of the second air pipe, the lower end of the second air pipe is located at the bottom center position in the culture barrel, the motor is installed on the barrel cover arranged on the upper end of the culture barrel, the output shaft of the motor is connected with the rotating rod inserted into the culture barrel, a plurality of stirring holes are formed in the detachable stirring plate installed on the lower end of the rotating rod, and a plurality of stirring blades are installed on the rotating rod from top to bottom. Compared with the prior art, the utility model has the beneficial effects that: during the culture of bacillus, air flow is introduced into the bottom of the culture barrel through the ring pipe, the second air pipe and the first air pipe, ensuring that air flow is introduced from the bottom of the culture liquid;The motor drives the rotating rod and the stirring plate to rotate, which can break the bubbles and mix the culture liquid thoroughly, improving the fermentation effect, but during fermentation, multiple sampling detection is required, and the detection result is not accurate by sampling detection through the discharge pipe (the residue of the last sampling on the outer side of the switch valve on the inner wall of the discharge pipe is mixed with the current sampling for detection). SUMMARY

[0005] The utility model aims at providing a culture device of bacillus cereus in sauce type Daqu to overcome the shortcomings of the prior art.

[0006] The utility model discloses a culture device of bacillus cereus in sauce-flavor daqu, including jar body, the bottom of jar body is provided with sampling pipe, the top of jar body is provided with feed pipe and speed reducer motor, the side wall of jar body is installed with discharge pipe near the bottom, install B valve on the discharge pipe, install stirring member in jar body, the speed reducer motor is used for driving stirring member rotation, the discharge end of sampling pipe is fixed with sample storage cylinder, sample storage cylinder communicates with sampling pipe, fixed with the baffle who is used for separating the inner chamber into A cavity and B cavity in sample storage cylinder, the A through -hole of communicating A cavity and B cavity is established to the baffle far from the center, the inner wall of sample storage cylinder is established annular groove far from sampling pipe, install the baffle in annular groove, install rotatable rubber plug between the baffle and the baffle, the rubber plug is tightly pressed on the baffle through the baffle, open B through -hole on the rubber plug, A through -hole is located the B through -hole's rotation track, still open square hole in the center of rubber plug.

[0007] Preferably, two limit blocks are fixed on the inner wall of the port of the sample storage cylinder, and a screw is screwed on the rubber plug and located between the two limit blocks.

[0008] Preferably, the stirring member includes a hollow shaft, the hollow shaft is fixed on the rotating shaft of the speed reducer motor, and the end of the hollow shaft away from the speed reducer motor is closed, the air inlet hole and the air outlet hole are opened on the hollow shaft and away from each other, the air inlet hole is close to the speed reducer motor, a drainage arm is also fixed on the hollow shaft, the drainage arm is a hollow structure, the air outlet hole is used for connecting the hollow shaft and the drainage arm, a liquid outlet is opened on the drainage arm, and the liquid outlet is located on the back of the rotation direction of the drainage arm.

[0009] Preferably, a plurality of round rods are fixed on the hollow shaft along the radial direction thereof, the round rods are located above the drainage arm, the plurality of round rods are divided into N groups distributed along the axis of the hollow shaft, N is an integer not less than two, and the round rods in each group are uniformly distributed around the circumference of the hollow shaft.

[0010] Preferably, the drainage arm is two, and the two drainage arms are symmetrically installed on the hollow shaft.

[0011] Preferably, a blade is also fixed on the hollow shaft along the radial direction thereof, the blade is in a spiral shape, the blade is located between the air inlet hole and the round rod, and the rotating blade is used for generating downward liquid flow.

[0012] Compared with the prior art, the utility model has the advantages that:

[0013] Firstly, the test tube is inserted into the B through hole, then the rubber plug is rotated to align the B through hole with the A through hole, at this time the sampling tube is opened, the culture solution enters the test tube, after the test tube is filled, the rubber plug is reversed to close the sampling tube, finally the test tube is pulled out, the culture solution is avoided to remain in the B through hole, the accuracy of the sampling detection result is improved. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a structure schematic view of the utility model;

[0015] Figure 2 It is a structure schematic view of the rubber plug; Figure 1

[0016] Figure 3 It is a structure schematic view of the rubber plug; Figure 2

[0017] Figure 4 It is a structure schematic view of the stirring piece;

[0018] Figure 5 It is a partial section schematic view of the utility model;

[0019] Figure 6 It is a partial enlarged schematic view of A in the utility model; Figure 5

[0020] In the drawing, 1 is a jar body, 2 is a speed reducer motor, 3 is a hollow shaft, 4 is an air inlet hole, 5 is an air outlet hole, 6 is a drainage arm, 7 is a liquid outlet, 8 is a round rod, 9 is a sampling tube, 10 is a blade, 11 is a sample storage cylinder, 12 is a partition, 13 is an A through hole, 14 is a check ring, 15 is a rubber plug, 16 is a B through hole, 17 is a square hole, 18 is a limiting block, 19 is a screw. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be described clearly and completely below in combination with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. The components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0022] Therefore, the following detailed description of the embodiments of the utility model provided in the drawings is not intended to limit the scope of the claimed utility model, but only represents selected embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0023] ​​​It should be noted that the embodiments and features in the embodiments of the present application can be combined with each other without conflict.

[0024] It should be noted that similar reference numerals and letters refer to similar items throughout the accompanying drawings, and thus, once an item is defined in one drawing, it is not necessary to further define and explain it in subsequent drawings.

[0025] In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship commonly understood by those skilled in the art, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second" and the like are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0026] In the description of the present application, it should also be noted that unless otherwise explicitly specified and limited, the terms "provided", "mounted", "connected", "linked" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be connected inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0027] As Figures 1-3As shown, a culture device for Bacillus cereus in a sauce-flavor Daqu, comprising a tank body 1 erected on the ground, a sampling pipe 9 arranged at the bottom of the tank body 1, a feed pipe and a speed reducer motor 2 arranged at the top of the tank body 1, a discharge pipe installed at the side wall of the tank body 1 close to the bottom, a B valve installed on the discharge pipe, a stirring piece installed in the tank body 1, the speed reducer motor 2 used for driving the stirring piece to rotate, a sample storage cylinder 11 fixed at the discharge end of the sampling pipe 9, the sample storage cylinder 11 communicated with the sampling pipe 9, a partition plate 12 fixed in the sample storage cylinder 11 for separating the inner cavity into an A cavity and a B cavity, an A through hole 13 communicated with the A cavity and the B cavity arranged at the partition plate 12 away from the center, an annular groove arranged at the inner wall of the sample storage cylinder 11 away from the sampling pipe 9, a check ring 14 installed in the annular groove, a rotatable rubber plug 15 installed between the check ring 14 and the partition plate 12, the rubber plug 15 tightly pressed on the partition plate 12 through the check ring 14, a B through hole 16 arranged on the rubber plug 15, the A through hole 13 located on the rotation track of the B through hole 16, and a square hole 17 arranged at the center of the rubber plug 15, the test tube is first inserted into the B through hole 16 (the tube opening of the test tube tightly abuts against the partition plate 12), the test tube is clamped by the elasticity of the rubber, then the rubber plug 15 is rotated to align the B through hole 16 with the A through hole 13 (a square pipe is inserted into the square hole 17, and the rubber plug 15 is rotated by cooperating with the wrench rotating the square pipe), at this time, the sampling pipe 9 is opened, the culture solution enters the test tube, after the test tube is filled, the rubber plug 15 is reversed to close the sampling pipe 9, and finally the test tube is pulled out, avoiding the culture solution remaining in the B through hole 16, and improving the accuracy of the sampling detection result.

[0028] In the embodiment, as shown in Figure 2 and 3 two limiting blocks 18 are fixed on the inner wall of the port of the sample storage cylinder 11, and a screw 19 is screwed on the rubber plug 15 and located between the two limiting blocks 18, so as to limit the rotation range of the rubber plug 15, avoiding manual observation of whether the test tube is aligned with the A through hole 13, and improving the operation convenience and reducing the labor intensity of workers.

[0029] In the embodiment, as shown in Figures 4-6 the stirring piece comprises a hollow shaft 3 fixed on the rotating shaft of the speed reducer motor 2, the end of the hollow shaft 3 away from the speed reducer motor 2 is closed, an air inlet hole 4 and an air outlet hole 5 are arranged on the hollow shaft 3 and away from each other, the air inlet hole 4 is close to the speed reducer motor 2, a flow guide arm 6 is also fixed on the hollow shaft 3, the flow guide arm 6 is a hollow structure, the air outlet hole 5 is used for connecting the hollow shaft 3 and the flow guide arm 6, a liquid outlet 7 is arranged on the flow guide arm 6, the liquid outlet 7 is located on the back of the rotation direction of the flow guide arm 6, the air inlet hole 4 is designed on the upper part of the hollow shaft 3 and communicated with the flow guide arm 6 through the air outlet hole 5, when the hollow shaft 3 rotates, the back of the rotation direction of the flow guide arm 6 forms a negative pressure in the mixed solution, so as to suck the air above the liquid surface into the hollow shaft 3 to form an air flow into the liquid surface, the bottom mixed solution is fully oxygenated, and the production efficiency of the acetic acid bacteria is improved.

[0030] In this embodiment, as Figures 4-6 As shown, several round rods 8 are fixed radially on the hollow shaft 3. The round rods 8 are located above the drainage arm 6. The round rods 8 are divided into N groups distributed along the axis of the hollow shaft 3, where N is an integer not less than two. The round rods 8 in each group are evenly distributed around the circumference of the hollow shaft 3. After air enters the mixture, it forms bubbles. The rotating round rods 8 break the bubbles into smaller bubbles, which increases the oxygen content of the mixture per unit volume and accelerates the growth of acetic acid bacteria.

[0031] In this embodiment, as Figures 4-6 As shown, there are two drainage arms 6, which are symmetrically installed on the hollow shaft 3. When the drainage arms 6 encounter resistance during rotation, the forces on both sides of the hollow shaft 3 are balanced by symmetrical arrangement.

[0032] In this embodiment, as Figures 4-6 As shown, a blade 10 is fixed along the radial direction of the hollow shaft 3. The blade 10 is spiral-shaped and located between the air inlet 4 and the round rod 8. The rotating blade 10 is used to generate a downward liquid flow, forming an up-and-down circulating liquid flow, preventing nutrients from sinking, making the nutrients in the upper and lower layers more uniform, and improving the growth of bacteria.

[0033] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A culture device for Bacillus cereus in Jiang-flavor Daqu, comprising a tank body (1), a sampling pipe (9) is arranged at the bottom of the tank body (1), a feed pipe and a speed reducer motor (2) are arranged at the top of the tank body (1), a discharge pipe is installed on the side wall of the tank body (1) close to the bottom, a B valve is installed on the discharge pipe, a stirring part is installed in the tank body (1), and the speed reducer motor (2) is used for driving the stirring part to rotate, characterized in that: The discharge end of the sampling pipe (9) is fixed with a sample storage cylinder (11), the sample storage cylinder (11) is communicated with the sampling pipe (9), the sample storage cylinder (11) is fixed with a partition plate (12) for separating the inner cavity into A cavity and B cavity, the partition plate (12) is provided with an A through hole (13) for communicating the A cavity and the B cavity away from the center, the inner wall of the sample storage cylinder (11) is provided with an annular groove away from the sampling pipe (9), the annular groove is installed with a check ring (14), the check ring (14) and the partition plate (12) are installed with a rotatable rubber plug (15), the rubber plug (15) is tightly pressed on the partition plate (12) through the check ring (14), the rubber plug (15) is provided with a B through hole (16), the A through hole (13) is located on the rotation track of the B through hole (16), and the center of the rubber plug (15) is also provided with a square hole (17). ​ 2. The culture device of Bacillus cereus in Jiang-flavor Daqu according to claim 1, characterized in that: The inner wall of the port of the sample storage cylinder (11) is fixed with two limiting blocks (18), the rubber plug (15) is screwed with a screw (19), and the screw (19) is located between the two limiting blocks (18).

3. The culture device of Bacillus cereus in Jiang-flavor Daqu according to claim 1, characterized in that: The stirring part includes a hollow shaft (3), the hollow shaft (3) is fixed on the rotating shaft of the speed reducer motor (2), one end of the hollow shaft (3) away from the speed reducer motor (2) is closed, the hollow shaft (3) is provided with an air inlet hole (4) and an air outlet hole (5) away from each other, the air inlet hole (4) is close to the speed reducer motor (2), the hollow shaft (3) is also fixed with a flow guide arm (6), the flow guide arm (6) is a hollow structure, the air outlet hole (5) is used for communicating the hollow shaft (3) and the flow guide arm (6), the flow guide arm (6) is provided with a liquid outlet (7), and the liquid outlet (7) is located on the back of the rotation direction of the flow guide arm (6).

4. The culture device of Bacillus cereus in Jiang-flavor Daqu according to claim 3, characterized in that: A plurality of round rods (8) are fixed on the hollow shaft (3) along the radial direction, the round rods (8) are located above the flow guide arm (6), a plurality of the round rods (8) are divided into N groups distributed along the axis of the hollow shaft (3), N is an integer not less than two, and the round rods (8) in each group are uniformly distributed around the circumference of the hollow shaft (3).

5. The culture device of Bacillus cereus in Jiang-flavor Daqu according to claim 3 or 4, characterized in that: The flow guide arm (6) is two, and the two flow guide arms (6) are symmetrically installed on the hollow shaft (3).

6. The culture device of Bacillus cereus in Jiang-flavor Daqu according to claim 4, characterized in that: The hollow shaft (3) is also fixed with a blade (10) along the radial direction, the blade (10) is spiral-shaped, the blade (10) is located between the air inlet hole (4) and the round rod (8), and the rotating blade (10) is used for generating downward liquid flow.

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