Automatic liquid separation and subpackaging system for nucleic acid freeze-dried beads

Abstract

The utility model discloses an automatic liquid separation and subpackaging system for nucleic acid freeze-dried beads, which comprises a base and a subpackaging mechanism, and a plurality of supports are fixedly mounted at the bottom of the base; the split charging mechanism comprises a motor fixedly installed at the upper end of the base, an intermittent transmission part is fixedly installed at the output end of the motor, a circular plate is fixedly connected to the intermittent transmission part, a plurality of limiting parts are fixedly installed at the upper end of the circular plate, an installation part is fixedly installed at the upper end of the base, and an electric push rod is fixedly installed on the installation part. A box body is fixedly mounted at the upper end of the mounting part, a discharging pipe which penetrates through the mounting part and is fixedly connected with the mounting part is fixedly mounted on the box body, an arc-shaped plate is fixedly connected to the output end of the electric push rod, a discharging part is fixedly connected to one end of the discharging pipe, and a fixing part opposite to the limiting part is fixedly mounted at the bottom of the arc-shaped plate. According to the system, by means of cooperation between the intermittent transmission part and the discharging part, nucleic acid freeze-dried beads can be automatically subpackaged, and the subpackaging efficiency of the nucleic acid freeze-dried beads is improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to nucleic acid freeze -drying bead technical field, concretely is a kind of nucleic acid freeze -drying bead automatic liquid separation and subpackaging system. BACKGROUND

[0002] In various nucleic acid related experiments and detection, such as polymerase chain reaction (PCR), gene sequencing, nucleic acid hybridization, the dosage of nucleic acid has accurate requirement, needs to be accurately distributed to each reaction system by liquid separation and subpackaging, to ensure the accuracy and repeatability of experimental results, nucleic acid freeze-dried beads are usually prepared in batches, and if not liquid separation and subpackaging, multiple experiments or samples share the same batch of freeze-dried beads, which may cause cross contamination between different samples due to improper operation. After liquid separation and subpackaging, each subpackaged nucleic acid freeze-dried bead is independent, which can effectively avoid the risk of cross contamination, ensure the integrity and purity of nucleic acid of each experiment or sample, and improve the reliability of experimental results.

[0003] During manual operation of liquid separation and subpackaging of nucleic acid freeze-dried beads, the operator's technical level, fatigue level and other factors may affect the accuracy of liquid separation and subpackaging, manual operation consumes a lot of labor, and the work efficiency of manual operation is low, which affects the subsequent work, and thus reduces the economic benefits of enterprises. Therefore, the present application provides a nucleic acid freeze-dried bead automatic liquid separation and subpackaging system. UTILITY MODEL CONTENT

[0004] In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a nucleic acid freeze-dried bead automatic liquid separation and subpackaging system, which effectively solves the problems of low liquid separation and subpackaging efficiency and poor automation of traditional nucleic acid freeze-dried bead automatic liquid separation and subpackaging systems.

[0005] To achieve the above purpose, the utility model provides the following technical scheme: a nucleic acid freeze-dried bead automatic liquid separation and subpackaging system, comprising a base and a subpackaging mechanism, a plurality of supports are fixedly installed at the bottom of the base;The subpackaging mechanism comprises a motor fixedly installed on the upper end of the base, an intermittent transmission member is fixedly installed at the output end of the motor, a circular plate is fixedly connected to the intermittent transmission member, a plurality of limiting members are fixedly installed at the upper end of the circular plate, an installation member is fixedly installed at the upper end of the base, an electric push rod is fixedly installed on the installation member, the output end of the electric push rod penetrates the installation member and is slidably connected thereto, a box body is fixedly installed at the upper end of the installation member, a discharge pipe is fixedly installed on the box body and penetrates the installation member and is fixedly connected to the installation member, the output end of the electric push rod is fixedly connected with an arc plate, a discharge member is fixedly connected to one end of the discharge pipe, the discharge member penetrates the arc plate and is fixedly connected thereto, and a fixed part is fixedly installed at the bottom of the arc plate, which is arranged opposite to the limiting part.

[0006] Preferably, the intermittent transmission part comprises a rotating rod fixedly connected with the motor output end, and an incomplete gear is fixedly installed on the rotating rod.

[0007] Preferably, a fixed rod is fixedly installed on the base, an output gear meshing with the incomplete gear is fixedly installed on the upper end of the fixed rod, and the upper end of the output gear is fixedly connected with the circular plate.

[0008] Preferably, the limiting part comprises an installation cylinder fixedly connected with the circular plate, an installation plate slidingly abutting against the inner wall of the installation cylinder is slidingly connected in the installation cylinder, springs are fixedly installed on the installation plate and the circular plate, and the installation cylinder is oppositely arranged with the fixing part and the discharging part.

[0009] Preferably, the mounting part comprises two vertical plates and a horizontal plate, the two vertical plates are fixedly installed on the upper end of the base, the horizontal plate is fixedly installed on the upper end of the two vertical plates, the box body is fixedly installed on the upper end of the horizontal plate, and the output end of the electric push rod penetrates through the horizontal plate and is slidingly connected with the horizontal plate.

[0010] Preferably, the discharging part comprises a hose penetrating through the arc-shaped plate and fixedly connected with the arc-shaped plate, one end of the hose is fixedly connected with the discharging pipe, the other end of the hose is fixedly connected with a throttle valve, the bottom of the throttle valve is fixedly installed with an inclined pipe, and the inclined pipe is oppositely arranged with the installation cylinder.

[0011] Preferably, the fixing part comprises a fixed column fixedly installed on the bottom of the arc-shaped plate, a limiting plate is fixedly installed on the bottom of the fixed column, an elastic buffer pad is fixedly installed on the bottom of the limiting plate, and the elastic buffer pad is oppositely arranged with the installation cylinder.

[0012] Compared with the prior art, the device has the beneficial effects that: through the setting of the sub-packaging mechanism, the cooperation between the intermittent transmission part and the limiting part can quickly move the sub-packaging container after completing sub-packaging and move another sub-packaging container to be opposite to the discharging part, thereby improving the efficiency of sub-packaging work and the automation degree of the device, the cooperation between the discharging part and the fixing part can quantitatively sub-packaging the sub-packaging container, and the nucleic acid freeze-dried beads overflowing from the sub-packaging container can be pressed back into the sub-packaging container, thereby reducing subsequent work, improving the efficiency of product production, and reducing the waste of labor. BRIEF DESCRIPTION OF DRAWINGS

[0013] The accompanying drawings are included to provide a further understanding of the present application, and constitute a part of the specification, illustrate the present application and are used to explain the present application together with the embodiments of the present application, and do not constitute a limitation on the present application.

[0014] In the drawings:

[0015] Figure 1 It is a structural schematic view of the nucleic acid freeze-dried bead automatic sub-liquid sub-packaging system.

[0016] Figure 2 This is a cross-sectional view of the automated dispensing and packaging system for nucleic acid lyophilized beads of this utility model;

[0017] Figure 3 This utility model Figure 1 Enlarged view of point A in the middle;

[0018] Figure 4 This utility model Figure 2 Enlarged view of point B in the middle;

[0019] Figure 5 This utility model Figure 2 Enlarged view of point C in the middle;

[0020] In the diagram: 1. Base; 2. Bracket; 3. Motor; 4. Rotating rod; 5. Mounting cylinder; 6. Box body; 7. Mounting component; 8. Discharge pipe; 9. Incomplete gear; 10. Electric push rod; 11. Arc plate; 12. Mounting plate; 13. Hose; 14. Throttling valve; 15. Inclined tube; 16. Limiting plate; 17. Elastic buffer pad; 18. Fixing rod; 19. Spring; 20. Driven gear; 21. Circular plate; 22. Fixing column. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0022] Depend on Figures 1-5 As shown, this utility model includes a base 1 and a disassembly mechanism. Multiple supports 2 are fixedly installed at the bottom of the base 1, and the multiple supports 2 can ensure the stability of the base 1. An installation component 7 is fixedly installed at the upper end of the base 1. The installation component 7 includes two vertical plates and a horizontal plate. The two vertical plates are fixedly installed at the upper end of the base 1, and the horizontal plate is fixedly installed at the upper end of the two vertical plates. The box body 6 is fixedly installed at the upper end of the horizontal plate. The output end of the electric push rod 10 passes through the horizontal plate and is slidably connected to it.

[0023] The dispensing mechanism includes a motor 3 fixedly installed on the upper end of the base 1. An intermittent transmission component is fixedly installed at the output end of the motor 3. The intermittent transmission component includes a rotating rod 4 fixedly connected to the output end of the motor 3. A reducer (not shown in the figure) is installed on the rotating rod 4. An incomplete gear 9 is fixedly installed on the rotating rod 4. A fixed rod 18 is fixedly installed on the base 1. A driven gear 20 that meshes with the incomplete gear 9 is fixedly installed at the upper end of the fixed rod 18. The upper end of the driven gear 20 is fixedly connected to the circular plate 21.

[0024] The intermittent transmission part is fixedly connected with a circular plate 21, the upper end of the circular plate 21 is fixedly installed with a plurality of limiting parts, the limiting parts include a mounting cylinder 5 fixedly connected with the circular plate 21, the mounting cylinder 5 is slidably connected with a mounting plate 12 abutting against the inner wall of the mounting cylinder 5, the mounting plate 12 is fixedly installed with a spring 19 on the circular plate 21, the mounting cylinder 5 can ensure the stability of the sub-packaging container, and the spring 19 can avoid the rigid collision of the nucleic acid freeze-dried beads falling into the sub-packaging container, so that the nucleic acid freeze-dried beads are prevented from being broken, and the mounting cylinder 5 is oppositely arranged with the fixing part and the discharging part;

[0025] Wherein, when working, the motor 3 is turned on, the output end of the motor 3 drives the rotating rod 4 to rotate, the rotating rod 4 drives the incomplete gear 9 to rotate, the incomplete gear 9 drives the driven gear 20 to rotate, the incomplete gear 9 is provided with a tooth, the incomplete gear 9 rotates one circle, and the driven gear 20 only rotates a certain angle, the driven gear 20 drives the circular plate 21 to rotate a certain angle, the angle is equal to the included angle between two adjacent limiting parts, the rotation of the circular plate 21 drives the limiting parts to rotate, and the two limiting parts are always oppositely arranged with the fixing part and the discharging part;

[0026] The mounting part 7 is fixedly installed with an electric push rod 10, the output end of the electric push rod 10 penetrates through the mounting part 7 and is slidably connected with the mounting part 7, the upper end of the mounting part 7 is fixedly installed with a box body 6, the box body 6 is fixedly installed with a discharging pipe 8 penetrating through the mounting part 7 and fixedly connected with the mounting part 7, the output end of the electric push rod 10 is fixedly connected with an arc-shaped plate 11, one end of the discharging pipe 8 is fixedly connected with a discharging part, the discharging part includes a hose 13 penetrating through the arc-shaped plate 11 and fixedly connected with the arc-shaped plate 11, one end of the hose 13 is fixedly connected with the discharging pipe 8, the other end of the hose 13 is fixedly connected with a throttle valve 14, the bottom of the throttle valve 14 is fixedly installed with an inclined pipe 15, the inclined pipe 15 can reduce the falling speed of the nucleic acid freeze-dried beads, and can avoid the nucleic acid freeze-dried beads from being broken due to too fast falling speed and contacting the sub-packaging container, and the inclined pipe 15 is oppositely arranged with the mounting cylinder 5;

[0027] The discharging part penetrates through the arc-shaped plate 11 and is fixedly connected with the arc-shaped plate 11, the bottom of the arc-shaped plate 11 is fixedly installed with a fixing part oppositely arranged with the limiting part, the fixing part includes a fixed column 22 fixedly installed at the bottom of the arc-shaped plate 11, the bottom of the fixed column 22 is fixedly installed with a limiting plate 16, the bottom of the limiting plate 16 is fixedly installed with an elastic buffer pad 17, the elastic buffer pad 17 can avoid the hard contact with the nucleic acid freeze-dried beads, so that the nucleic acid freeze-dried beads are prevented from being damaged due to the hard contact, and the elastic buffer pad 17 is oppositely arranged with the mounting cylinder 5;

[0028] Wherein, when dispensing, the electric push rod 10 is opened, the output end of the electric push rod 10 drives the arc plate 11 to move, the arc plate 11 drives the hose 13 to move, the hose 13 drives the throttle valve 14 to move, the throttle valve 14 drives the inclined pipe 15 to move, the inclined pipe 15 moves and is oppositely arranged with the dispensing container, the throttle valve 14 is opened, the nucleic acid freeze-dried beads are transferred from the box body 6 into the discharge pipe 8, and then transferred from the discharge pipe 8 into the hose 13, finally the nucleic acid freeze-dried beads drop into the dispensing container through the throttle valve 14, at the same time, the arc plate 11 drives the fixed column 22 to move, the fixed column 22 drives the limiting plate 16 to move, the limiting plate 16 drives the elastic buffer pad 17 to move, the elastic buffer pad 17 moves and abuts against the nucleic acid freeze-dried beads, the nucleic acid freeze-dried beads overflowing from the dispensing container are pressed back into the dispensing container, facilitating subsequent workers to seal the dispensing container.

[0029] Working principle: when working, the electric push rod 10 and the motor 3 are opened at the same time, the output end of the motor 3 drives the rotating rod 4 to rotate, the rotating rod 4 drives the incomplete gear 9 to rotate, the incomplete gear 9 drives the driven gear 20 to rotate, the incomplete gear 9 is provided with a tooth, the incomplete gear 9 rotates one circle and the driven gear 20 only rotates a certain angle, the driven gear 20 drives the circular plate 21 to rotate a certain angle, the angle is equal to the included angle generated by two adjacent limiting pieces, the rotation of the circular plate 21 drives the limiting pieces to rotate, and the two limiting pieces are always oppositely arranged with the fixed piece and the discharge piece;

[0030] At the same time, the output end of the electric push rod 10 drives the arc plate 11 to move, the arc plate 11 drives the hose 13 to move, the hose 13 drives the throttle valve 14 to move, the throttle valve 14 drives the inclined pipe 15 to move, the inclined pipe 15 moves and is oppositely arranged with the dispensing container, the throttle valve 14 is opened, the nucleic acid freeze-dried beads are transferred from the box body 6 into the discharge pipe 8, and then transferred from the discharge pipe 8 into the hose 13, finally the nucleic acid freeze-dried beads drop into the dispensing container through the throttle valve 14, at the same time, the arc plate 11 drives the fixed column 22 to move, the fixed column 22 drives the limiting plate 16 to move, the limiting plate 16 drives the elastic buffer pad 17 to move, the elastic buffer pad 17 moves and abuts against the nucleic acid freeze-dried beads, the nucleic acid freeze-dried beads overflowing from the dispensing container are pressed back into the dispensing container, facilitating subsequent workers to seal the dispensing container, the above-mentioned work driven by the motor 3 and the electric push rod 10 is repeated, and the dispensing of the nucleic acid freeze-dried beads can be completed.

Claims

1. An automatic nucleic acid lyophilized bead dispensing system, comprising a base (1) and a dispensing mechanism, characterized in that: The bottom of the base (1) is fixedly provided with a plurality of supports (2); the sub-packaging mechanism comprises a motor (3) fixedly arranged at the upper end of the base (1), an intermittent transmission member fixedly arranged at the output end of the motor (3), a circular plate (21) fixedly connected to the intermittent transmission member, a plurality of limiting members fixedly arranged at the upper end of the circular plate (21), a mounting member (7) fixedly arranged at the upper end of the base (1), an electric push rod (10) fixedly arranged at the mounting member (7), an output end of the electric push rod (10) penetrating through the mounting member (7) and being slidably connected to the mounting member (7), a box body (6) fixedly arranged at the upper end of the mounting member (7), a discharge pipe (8) fixedly arranged at the box body (6) and penetrating through the mounting member (7) and being fixedly connected to the mounting member (7), an arc-shaped plate (11) fixedly connected to the output end of the electric push rod (10), a discharging member fixedly connected to one end of the discharge pipe (8), the discharging member penetrating through the arc-shaped plate (11) and being fixedly connected to the arc-shaped plate (11), and a fixing member fixedly arranged at the bottom of the arc-shaped plate (11) and oppositely arranged to the limiting member.

2. The nucleic acid lyophilized bead automated aliquoting system of claim 1, wherein: The intermittent transmission member comprises a rotating rod (4) fixedly connected to the output end of the motor (3), and an incomplete gear (9) fixedly arranged at the rotating rod (4).

3. The nucleic acid lyophilized bead automated aliquoting system of claim 1, wherein: The base (1) is fixedly provided with a fixing rod (18), the upper end of the fixing rod (18) is fixedly provided with a driven gear (20) engaged with the incomplete gear (9), and the upper end of the driven gear (20) is fixedly connected to the circular plate (21).

4. The nucleic acid lyophilized bead automated aliquoting system of claim 1, wherein: The limiting member comprises a mounting cylinder (5) fixedly connected to the circular plate (21), a mounting plate (12) slidably connected to the mounting cylinder (5) and abutting against the inner wall of the mounting cylinder (5), a spring (19) fixedly arranged at the mounting plate (12) and the circular plate (21), and the mounting cylinder (5) oppositely arranged to the fixing member and the discharging member.

5. The nucleic acid lyophilized bead automated aliquoting system of claim 1, wherein: The mounting member (7) comprises two vertical plates fixedly arranged at the upper end of the base (1), and a horizontal plate fixedly arranged at the upper end of the two vertical plates, the box body (6) is fixedly arranged at the upper end of the horizontal plate, and the output end of the electric push rod (10) penetrates through the horizontal plate and is slidably connected to the horizontal plate.

6. The nucleic acid lyophilized bead automated aliquoting system of claim 1, wherein: The discharging member comprises a hose (13) penetrating through the arc-shaped plate (11) and fixedly connected to the arc-shaped plate (11), one end of the hose (13) is fixedly connected to the discharge pipe (8), the other end of the hose (13) is fixedly connected to a throttle valve (14), the bottom of the throttle valve (14) is fixedly provided with an inclined pipe (15), and the inclined pipe (15) is oppositely arranged to the mounting cylinder (5).

7. The nucleic acid lyophilized bead automated aliquoting system of claim 1, wherein: The fixing member comprises a fixing column (22) fixedly arranged at the bottom of the arc-shaped plate (11), a limiting plate (16) fixedly arranged at the bottom of the fixing column (22), a spring buffer (17) fixedly arranged at the bottom of the limiting plate (16), and the spring buffer (17) oppositely arranged to the mounting cylinder (5).

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