Fe3O4 (at) AuNPs magnetic molecular imprinting sensor detection system

Abstract

The utility model relates to the technical field of protein detection, and discloses a Fe3O4 (at) AuNPs magnetic molecular imprinting sensor detection system which comprises an electrochemical workstation, a magnetic electrochemical molecular imprinting sensor, a carrier, a container, a metal disc and an alternating electric field generation module, the magnetic electrochemical molecular imprinting sensor comprises a working electrode, a counter electrode and a reference electrode, the working electrode, the counter electrode and the reference electrode are all connected with the electrochemical workstation, the working electrode is partially inserted into the container, the metal disc is arranged at the bottom of the container, and the positive electrode of the alternating electric field generating module is connected with the working electrode. The negative electrode of the alternating electric field generation module is connected with the metal disc, the carrier is arranged at the bottom of the working electrode, the carrier is a Fe3O4 (at) AuNPs carrier, and the Fe3O4 (at) AuNPs carrier is obtained by combining a molecularly imprinted polymer of protein with Fe3O4 and then combining with AuNPs. According to the utility model, the detection speed, the detection range and the detection effect of protein are improved.

Description

Technical Field

[0001] This invention relates to the field of protein detection technology, and in particular to a Fe3O4@AuNPs magnetic molecular imprint sensor detection system. Background Technology

[0002] Currently, existing protein detection technologies suffer from limitations due to the unstable binding of molecularly imprinted polymers during electrodeposition, limiting the detection range to only molecularly imprinted polymers and resulting in cumbersome experimental procedures. For example, CN114577879A discloses a protein detection system based on electrophoresis and molecular imprinting principles. This system comprises an electrochemical molecularly imprinted sensor and an electrophoresis apparatus. The electrophoresis apparatus is an electrolytic cup with a Pt disk at the bottom. The electrochemical molecularly imprinted sensor is suspended within the electrolytic cup via a fixed stage. The Pt disk and the electrochemical molecularly imprinted sensor are connected to the two electrodes of a power supply, thereby achieving targeted enrichment of the analyte at the sensing interface mediated by the electric field. It utilizes electrodeposition to fix the molecularly imprinted polymer onto the surface of a glassy carbon electrode, thus achieving polymer imprinting. Utility Model Content

[0003] In view of the shortcomings of the prior art described above, the purpose of this utility model is to provide a Fe3O4@AuNPs magnetic molecular imprint sensor detection system, which for the first time combines magnetic molecular imprinting technology with alternating electrophoresis technology. It adopts the "soft deposition" method and uses Fe3O4 magnetic beads and AuNPs as carriers to achieve the imprinting of molecular polymers, and simplifies the experimental procedure.

[0004] The present invention adopts the following technical solution:

[0005] A Fe3O4@AuNPs magnetic molecular imprinted sensor detection system includes an electrochemical workstation, a magnetic electrochemical molecular imprinted sensor, a carrier, a container, a metal disk, and an alternating electric field generating module. The magnetic electrochemical molecular imprinted sensor includes a working electrode, a counter electrode, and a reference electrode, all connected to the electrochemical workstation. The working electrode is partially inserted into the container, and the metal disk is located at the bottom of the container. The positive electrode of the alternating electric field generating module is connected to the working electrode, and the negative electrode is connected to the metal disk. The carrier is located at the bottom of the working electrode and is a Fe3O4@AuNPs carrier obtained by binding Fe3O4 to Fe3O4 and then to AuNPs via a protein molecular imprinted polymer.

[0006] Preferably, the Fe3O4@AuNPs magnetic molecular imprint sensor detection system further includes a fixed stage, through which the working electrode is suspended in the container.

[0007] Preferably, in the above-mentioned Fe3O4@AuNPs magnetic molecular imprint sensor detection system, the metal disk is a platinum disk.

[0008] Preferably, in the above-mentioned Fe3O4@AuNPs magnetic molecular imprint sensor detection system, the working electrode is a magnetic glassy carbon electrode.

[0009] Preferably, in the above-mentioned Fe3O4@AuNPs magnetic molecular imprint sensor detection system, the counter electrode is a glass wire electrode.

[0010] Preferably, in the above-mentioned Fe3O4@AuNPs magnetic molecular imprint sensor detection system, the reference electrode is an Ag / AgCl electrode.

[0011] Preferably, the Fe3O4@AuNPs magnetic molecular imprint sensor detection system further includes a magnet, which is positioned corresponding to the working electrode and is used to adsorb the carrier.

[0012] Preferably, in the above-mentioned Fe3O4@AuNPs magnetic molecular imprint sensor detection system, the magnet is an Nd2Fe14B magnet.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0014] This invention can be applied to the detection of various protein molecules. It involves combining a molecularly imprinted polymer of the protein with Fe3O4, and then with AuNPs to form a complex with Fe3O4@AuNPs as the carrier. This complex is adsorbed onto a magnetic glassy carbon electrode. After adsorption, the protein to be detected is added. Under the influence of an electric field, the protein rapidly binds to the molecularly imprinted polymer. The addition of magnetism also enables rapid magnetic separation during detection, reducing experimental time. The addition of magnetic beads (Fe3O4) also ensures good experimental reproducibility and facilitates the separation of impurities. It exhibits strong magnetic responsiveness and performs excellently in complex experimental environments. The addition of AuNPs prevents the aggregation of magnetic nanoparticles and provides beneficial properties such as improved biocompatibility, chemical inertness, high surface energy, optical and electronic characteristics. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0016] Figure 1 This is a structural diagram of a Fe3O4@AuNPs magnetic molecular imprint sensor detection system according to an embodiment of the present invention;

[0017] Figure 2 This is a structural diagram of a magnetic electrochemical molecularly imprinted sensor according to an embodiment of the present invention.

[0018] Explanation of reference numerals in the attached figures:

[0019] 1. Electrochemical workstation; 2. Magnetic electrochemical molecular imprinted sensor; 201. Working electrode; 202. Counter electrode; 203. Reference electrode; 3. Carrier; 4. Container; 5. Metal disk; 6. Alternating electric field generation module; 7. Magnet. Detailed Implementation

[0020] The following specific examples illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this utility model. It should be noted that, unless otherwise specified, the following embodiments and features described therein can be combined with each other.

[0021] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0022] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0023] The present invention will now be further described with reference to the accompanying drawings.

[0024] This utility model provides a Fe3O4@AuNPs magnetic molecular imprint sensor detection system, such as... Figure 1 and Figure 2 As shown, the Fe3O4@AuNPs magnetic molecular imprinted sensor detection system includes an electrochemical workstation 1, a magnetic electrochemical molecular imprinted sensor 2, a carrier 3, a container 4, a metal disk 5, and an alternating electric field generating module 6. The magnetic electrochemical molecular imprinted sensor 2 includes a working electrode 201, a counter electrode 202, and a reference electrode 203, all connected to the electrochemical workstation 1. The working electrode 201 is partially inserted into the container 4. The metal disk 5 is located at the bottom of the container 4. The positive electrode of the alternating electric field generating module 6 is connected to the working electrode 201, and the negative electrode is connected to the metal disk 5. The carrier 3 is located at the bottom of the working electrode 201 and is a Fe3O4@AuNPs carrier. The Fe3O4@AuNPs carrier is obtained by binding Fe3O4 to a protein molecularly imprinted polymer and then to AuNPs.

[0025] In this embodiment, the carrier 3 serves as a modification layer for the working electrode 201. In practice, the protein to be detected is added to the container 4, and the alternating electric field generation module 6 is activated to generate an electric field within the container 4. The protein to be detected is driven by the upward electric field force to the surface of the working electrode 201 for enrichment, allowing the protein to bind more efficiently to the Fe3O4@AuNPs carrier. The changes in electrical parameters in the circuit formed by the working electrode 201 and the counter electrode 202 are detected by the electrochemical workstation 1. Based on these changes in electrical parameters, the presence of the target protein or the concentration of the target protein can be determined.

[0026] It should be noted that the electrochemical workstation 1 is both a power source providing voltage and current and a testing instrument capable of accurately measuring voltage and current. Structurally, it can be considered that an ammeter is connected between the working electrode 201 and the counter electrode 202, and a voltmeter is connected between the working electrode 201 and the reference electrode 203. The three electrodes form two circuits. The circuit formed by the working electrode 201 and the reference electrode 203 carries a very small current and is used to test the electrode potential; while the circuit formed by the working electrode 201 and the counter electrode 202 is used to test the current. Based on the measured current, protein detection is achieved. For example, a standard curve of current change (ΔI) versus target protein concentration can be established to achieve quantitative detection.

[0027] The Fe3O4@AuNPs support serves as the modification layer for the working electrode 201. Within the Fe3O4@AuNPs support, Fe3O4 magnetic nanoparticles achieve rapid adsorption / desorption of the complex via an external magnetic field, simplifying electrode modification and sample separation. This provides more molecularly imprinted polymer (MIP) loading sites, enhancing the target molecule capture efficiency. AuNPs are gold nanoparticles; the high electron transfer efficiency of gold optimizes the electrochemical signal response. Simultaneously, AuNPs facilitate stable immobilization of protein molecules, reducing the risk of denaturation and thus improving biocompatibility. Furthermore, they can couple the MIP layer via Au-S bonds or electrostatic interactions. The role of the molecularly imprinted polymer (MIP) lies in its specific recognition and anti-interference capabilities. Specifically, on the one hand, after elution of the template protein, it forms a cavity structure complementary to the target protein, achieving a "lock-key" binding. On the other hand, it can selectively adsorb the target protein and inhibit the non-specific binding of other analogues (such as isomers and degradation products).

[0028] The alternating electric field generating module 6 can be selected as an alternating electric field electrophoresis apparatus. When using this alternating electric field electrophoresis apparatus to generate alternating current, the forward electrophoresis time is longer than the reverse electrophoresis time. Adjust the required time, then turn on the working switch, adjust the output selection knob to the required current and voltage values, and after the electrophoresis is completed, turn the output selection knob back to the 0 position and turn off the power switch.

[0029] In some embodiments, the Fe3O4@AuNPs magnetic molecular imprint sensor detection system further includes a fixed stage, through which the working electrode 201 is suspended in the container 4.

[0030] Of course, the working electrode 201 can also be suspended and fixed in the container 4 in other ways, such as by providing a clamping assembly in the upper part of the container 4. The clamping assembly includes, but is not limited to, a clamp that can be used to clamp the electrode.

[0031] In some embodiments, the metal disk 5 is a platinum disk.

[0032] In some embodiments, the working electrode 201 is a magnetic glassy carbon electrode, the counter electrode 202 is a glass filament electrode, and the reference electrode 203 is an Ag / AgCl electrode.

[0033] The function of the working electrode 201 is to facilitate the specific recognition and electrochemical reaction of the target molecule. In this embodiment, a magnetic glassy carbon electrode is selected as the working electrode 201, which facilitates the control of the imprint material's fixation or separation via an external magnetic field. Magnetic materials can enhance electron transfer efficiency and improve detection sensitivity.

[0034] The function of the counter electrode 202 is to form a closed circuit and balance the current generated by the redox reaction occurring on the working electrode. In this embodiment, a glass wire electrode is chosen as the counter electrode 202 to avoid interfering with the reaction. Of course, the counter electrode 202 can also be made of other materials, such as platinum wire or carbon electrode.

[0035] The reference electrode 203 serves to provide a stable potential reference, ensuring the accuracy of the working electrode potential measurement.

[0036] In some embodiments, such as Figure 1 As shown, the Fe3O4@AuNPs magnetic molecular imprint sensor detection system also includes a magnet 7, which is positioned corresponding to the working electrode 201 to adsorb the carrier 3. For example, the magnet 7 can be disposed inside the working electrode 201.

[0037] In some embodiments, the magnet is an Nd2Fe14B magnet.

[0038] In summary, compared to existing methods that use electrodeposition to deposit molecularly imprinted polymers onto a glassy carbon electrode and then apply a DC electric field for protein detection, this invention eliminates the electrodeposition process. It employs a "soft deposition" method, utilizing a magnetic glassy carbon electrode (MGCE) to adsorb the MIP@Fe3O4@AuNPs molecularly imprinted polymer. After adding the target protein to the solution, an electric field enhancement method is used to accelerate the detection speed and simultaneously enrich the target protein, improving detection sensitivity. Furthermore, the use of an alternating electric field enhances the anti-interference capability of the substance detection and increases its specificity.

[0039] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model, and they should all be covered within the scope of the claims and specification of this utility model.

Claims

1. A Fe3O4@AuNPs magnetic molecularly imprinted sensor detection system, characterized in that, The application relates to an electrochemical workstation, a magnetic electrochemical molecular imprinting sensor, a carrier, a container, a metal disc and an alternating electric field generating module; wherein the magnetic electrochemical molecular imprinting sensor comprises a working electrode, a counter electrode and a reference electrode, the working electrode, the counter electrode and the reference electrode are connected with the electrochemical workstation, the working electrode is partially inserted into the container, the metal disc is arranged at the bottom of the container, the positive pole of the alternating electric field generating module is connected with the working electrode, the negative pole of the alternating electric field generating module is connected with the metal disc, the carrier is arranged at the bottom of the working electrode, the carrier is an Fe3O4@AuNPs carrier, the Fe3O4@AuNPs carrier is obtained by combining Fe3O4 with AuNPs through the molecular imprinting polymer of a protein.

2. The Fe304@AuNPs magnetic molecularly imprinted sensor detection system according to claim 1, characterized in that, The application further comprises a fixing table, the working electrode is hung in the container through the fixing table.

3. The Fe304@AuNPs magnetic molecularly imprinted sensor detection system according to claim 1, characterized in that, The metal disc is a platinum disc.

4. The Fe304@AuNPs magnetic molecularly imprinted sensor detection system according to claim 1, characterized in that, The working electrode is a magnetic glassy carbon electrode.

5. The Fe304@AuNPs magnetic molecularly imprinted sensor detection system according to claim 1, characterized in that, The counter electrode is a glass fiber electrode.

6. The Fe304@AuNPs magnetic molecularly imprinted sensor detection system according to claim 1, characterized in that, The reference electrode is an Ag / AgCl electrode.

7. The Fe304@AuNPs magnetic molecularly imprinted sensor detection system according to claim 1, characterized in that, The application further comprises a magnet, the magnet is arranged at the position of the working electrode and used for adsorbing the carrier. 8.The Fe3O4@AuNPs magnetic molecularly imprinted sensor detection system according to claim 7, characterized in that, The magnet is an Nd2Fe14B magnet.

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