Membrane material device for directional attachment of cells

Abstract

The utility model provides a cell directional attachment membrane material device which comprises an outer chamber, an inner chamber, an inner chamber, an outer chamber and an inner chamber, wherein the top of the outer chamber is provided with an outer chamber opening; the inner chamber is nested in the outer chamber, the diaphragm material is placed in the inner chamber, a fixing part is arranged at the bottom of the inner chamber, the diaphragm material is fixed in the inner chamber through the fixing part, an inner chamber opening is formed in the top of the inner chamber, and the inner chamber opening is formed in the outer chamber opening; and the upper cover covers the top of the outer chamber and is used for sealing the opening of the outer chamber. The fixing part is arranged at the bottom of the inner chamber, and the membrane material is fixed in the inner chamber through the fixing part, so that the risk that the membrane material floats or moves or the specific surface is changed due to the flowing environment of the cell culture fluid is reduced, and the experimental risk is reduced. The upper cover can seal the opening of the outer chamber, so that the cell directional attachment membrane material device is prevented from being polluted.

Description

Technical Field

[0001] This application relates to the field of biotechnology, and more particularly to a device for directional cell attachment of membrane materials. Background Technology

[0002] Cell sheet technology plays a crucial role in tissue engineering, allowing cells to grow without scaffold materials and enzymatic digestion. By stimulating extracellular matrix secretion, it forms dense sheet tissues, effectively repairing tissue defects and improving organ function. Cell attachment to a specific side of the sheet material helps construct tissues and organs with specific structures and functions. However, in current techniques, sheet materials are typically cut to the appropriate size, positioned with the side to be attached facing upwards in the cell culture medium, and then seeded. However, due to the fluidity of the cell culture medium, the sheet material may float on the culture plate, changing its position and potentially altering the specific side to be attached, increasing experimental risks. Summary of the Invention

[0003] This application provides a device for targeted cell attachment of membrane materials to solve the problems existing in related technologies. The technical solution is as follows:

[0004] This application provides a device for targeted cell attachment of membrane materials, comprising:

[0005] An outer chamber, the top of which has an outer chamber opening;

[0006] An inner chamber is nested inside an outer chamber. A diaphragm material is placed inside the inner chamber. The bottom of the inner chamber has a fixing part, through which the diaphragm material is fixed inside the inner chamber. The top of the inner chamber has an inner chamber opening, which is located inside the outer chamber opening.

[0007] A top cover that fits over the top of the outer chamber and is used to seal the opening of the outer chamber.

[0008] In one embodiment, a gel layer is laid at the bottom of the inner chamber, a specific side of the membrane material is positioned facing upwards, and the other side of the membrane material opposite to the specific side is adhered to the gel layer.

[0009] In one embodiment, the inner chamber includes a bottom plate and four side plates connected in sequence. The bottom of each of the four side plates is connected to the bottom plate, and the bottom of each of the four side plates forms a fixing portion between itself and the bottom plate. The edge of the diaphragm material is placed inside the fixing portion.

[0010] In one embodiment, the bottom of each of the four side panels is bent away from the center of the inner chamber relative to the top of the side panels, forming a curved portion, and an angled space is formed between the curved portion and the bottom plate, the angled space forming the fixing portion.

[0011] In one embodiment, each of the four side panels is provided with a mesh structure, and the interior of the inner chamber is connected to the interior of the outer chamber through the mesh structure.

[0012] In one embodiment, the mesh size of the mesh structure is less than 5 μm.

[0013] In one embodiment, a liquid circulation assembly is fixed to the bottom of the outer chamber.

[0014] In one embodiment, the liquid circulation component is a magnetic propeller.

[0015] In one embodiment, the top edge of the inner chamber has a handle that can be mounted on the top edge of the outer chamber.

[0016] In one embodiment, the culture medium is a polystyrene material that has not undergone TC treatment.

[0017] The advantages or beneficial effects of the above technical solutions include at least the following:

[0018] The cell-directed attachment membrane material device of this application embodiment includes an outer chamber, an inner chamber, and a top cover. The inner chamber is nested inside the outer chamber, and the membrane material is placed inside the inner chamber. Because the bottom of the inner chamber has a fixing part, the membrane material is fixed inside the inner chamber by the fixing part, reducing the risk of the membrane material floating or shifting due to the flow environment of the cell culture medium, thus reducing experimental risks. Because the top cover can seal the opening of the outer chamber, contamination of the cell-directed attachment membrane material device is prevented.

[0019] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of this application will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description

[0020] In the accompanying drawings, unless otherwise specified, the same reference numerals throughout the various drawings denote the same or similar parts or elements. These drawings are not necessarily drawn to scale. It should be understood that these drawings depict only some embodiments disclosed in this application and should not be construed as limiting the scope of this application.

[0021] Figure 1 A cross-sectional view of a device for directional attachment of membrane materials to cells;

[0022] Figure 2 This is a sectional view of the interior room;

[0023] Explanation of reference numerals in the attached figures:

[0024] 1. Outer chamber; 2. Inner chamber; 3. Top cover; 4. Fixing part; 21. Base plate; 22. Side plate; 23. Bending part; 24. Mesh structure; 5. Magnetic propeller; 25. Handle. Detailed Implementation

[0025] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of this application. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0026] like Figure 1 , Figure 2 As shown in the figure, this application provides a device for directional attachment of cell-specific membrane materials, including an outer chamber 1, an inner chamber 2, and a top cover 3. The outer chamber 1 has an opening at its top; the inner chamber 2 is nested inside the outer chamber 1, and the membrane is placed inside the inner chamber 2. The bottom of the inner chamber 2 has a fixing part 4, through which the membrane material is fixed to the inside of the inner chamber 2. The top of the inner chamber 2 has an opening that is located inside the opening of the outer chamber. The top cover 3 is placed on top of the outer chamber 1 to seal the outer chamber opening.

[0027] The cell-directed attachment membrane material device of this application embodiment includes an outer chamber 1, an inner chamber 2, and a top cover 3. The inner chamber 2 is nested inside the outer chamber 1, and the membrane material is placed inside the inner chamber 2. Because the bottom of the inner chamber 2 has a fixing part 4, the membrane material is fixed inside the inner chamber 2 by the fixing part 4, reducing the risk of the membrane material floating or shifting due to the flow environment of the cell culture medium, thus reducing experimental risks. Since the top cover 3 can seal the opening of the outer chamber, contamination of the cell-directed attachment membrane material device is prevented.

[0028] In one embodiment, to fix the diaphragm material, the inner chamber 2 includes a base plate 21 and four side plates 22 connected in sequence, with the bottom of each of the four side plates 22 connected to the base plate 21. A fixing portion 4 is formed between the bottom of each of the four side plates 22 and the base plate 21, and the edge of the diaphragm material is placed inside the fixing portion 4. Preferably, the four side plates 22 and the base plate 21 of the inner chamber 2 can be configured as an integrally formed structure.

[0029] Furthermore, the bottom of the four side plates 22 bends away from the center of the inner chamber 2 relative to the top of the side plates 22, forming a curved portion 23. There is an angled space between the curved portion 23 and the bottom plate 21, and the angled space forms a fixing portion 4.

[0030] Specifically, the curved portions 23 of the four side plates 22 form four angled spaces with the bottom plate 21. After the diaphragm material is placed in the inner chamber 2, the edge of the diaphragm material is placed in the four angled spaces, which can effectively fix the diaphragm material and fix the entire diaphragm material to the bottom of the inner chamber 2.

[0031] In one embodiment, to further secure the membrane material, a gel layer is laid at the bottom of the inner chamber 2, with a specific side of the membrane material facing upwards, and the opposite side of the membrane material adhering to the gel layer. The adhesiveness of the gel layer can improve the fixation of the membrane material and reduce the risk of the membrane material floating or shifting due to the flow environment of the cell culture medium, or changes in the specific side.

[0032] In one embodiment, each of the four side plates 22 is provided with a mesh structure 24, and the interior of the inner chamber 2 is connected to the interior of the outer chamber 1 through the mesh structure 24. When the culture medium required for cell growth is added to the outer chamber 1, the liquid in the outer chamber 1 and the inner chamber 2 can be exchanged through the mesh structure 24. Preferably, the pore size of the mesh structure 24 is less than 5 μm, allowing the culture medium to pass through the mesh structure 24. The culture medium is an existing polystyrene material that has not undergone TC treatment. Due to the hydrophobic properties of the mesh structure 24 on the inner chamber 2 and the culture medium, the cell adhesion characteristics are reduced, so that the seeded cells will not adhere to the inner wall of the inner chamber 2. Furthermore, because the bottom of the membrane material of the inner chamber 2 is coated with gel, cell adhesion is not easy.

[0033] In one embodiment, to accelerate the liquid circulation between the outer chamber 1 and the inner chamber 2, a liquid circulation assembly is fixed to the bottom of the outer chamber 1. Specifically, the liquid circulation assembly is a magnetic propeller 5. This magnetic propeller 5 is a conventional product; it is a device that uses magnetic force to drive the propeller to rotate, enabling contactless transmission. The magnetic propeller 5 can be fixed to the bottom of the outer chamber 1 by bolts or the like.

[0034] Since the bottom of the outer chamber 1 is equipped with a magnetic propeller 5, when the cell orientation attachment membrane material device is placed on the external magnetic device, the magnetic propeller 5 can be driven to rotate by the magnetic device, thereby circulating the cell culture medium inside the outer chamber 1 and the inner chamber 2. This allows cells that may fall into the outer chamber 1 to enter the inner chamber 2 through the mesh structure 24 of the inner chamber 2 and then attach to the membrane material at the bottom of the inner chamber 2, thereby reducing the risk of cell loss caused by cells attaching to locations outside the membrane material.

[0035] In one embodiment, the inner chamber 2 is coaxially nested inside the outer chamber 1, and the inner chamber 2 is lower than the opening of the outer chamber 1. To secure the inner chamber 2 inside the outer chamber 1, a handle 25 is provided at the top edge of the inner chamber 2, which can be positioned at the top edge of the outer chamber 1. The handle 25 allows the inner chamber 2 to be fixed inside the outer chamber 1, and the handle 25 can be grasped with tweezers, facilitating the separation of the inner chamber 2 and the outer chamber 1. Preferably, the handle 25 is located at the top edge of the side plate 22, and the handle 25 and the side plate 22 can be integrally formed.

[0036] When using the cell-directed attachment membrane material device of this application embodiment, a gel layer is first laid on the bottom plate 21 of the inner chamber 2, and then the membrane material is cut to the corresponding size of the bottom of the inner chamber 2. The specific side of the cell to be attached is selected to face the direction, and the other side opposite to the specific side is adhered to the gel layer, thereby fixing the membrane material to the bottom of the inner chamber 2.

[0037] Next, according to the experimental requirements, a certain amount of cells are prepared into a suspension and added to the inner chamber 2, so that the cells can bind to and attach to a specific surface of the membrane material at the bottom of the inner chamber 2 for growth.

[0038] The inner chamber 2 is then nested inside the outer chamber 1, and the culture medium required for cell growth is added to the outer chamber 1. The liquids in the outer chamber 1 and the inner chamber 2 can exchange through the mesh structure 24. Next, the top cover 3 is placed on the outer chamber 1 to seal the opening of the outer chamber, creating a sterile environment for culture.

[0039] After cells have been cultured for a period of time, the cell-directed attachment membrane material device can be placed on an external magnetic device, which drives the magnetic propeller 5 to rotate. The rotation speed of the magnetic propeller 5 can be adjusted via the magnetic device. After rotation, the magnetic propeller 5 moves the cell culture medium inside the outer chamber 1, circulating and exchanging it with the cell culture medium inside the inner chamber 2. This allows cells that may be present in the cell culture medium inside the outer chamber 1 to pass through the mesh structure 24 on the inner chamber 2 and return to the inner chamber 2, where they reattach to the membrane material for growth. After the cell culture medium has circulated for a period of time, the magnetic propeller 5 is turned off, and the culture process can continue.

[0040] The mesh structure 24 of this embodiment can be directly fixed to the side plate 22 of the inner chamber 2 by screws or the like, or the mesh structure 24 can be directly drilled out of the side plate 22 of the inner chamber 2. The upper cover 3 can be directly connected to the inner chamber 2 by threads, and the upper cover 3 can also be directly covered on the outer chamber 1.

[0041] In existing technologies, experiments require the precise amount of cells to be seeded and attached to a membrane material. However, the membrane material, due to its required size, leaves pores between the cell culture wells, allowing cells to easily fall into the cell culture plate at the bottom of the membrane material and adhere. Furthermore, due to the fluidity of the cell culture medium, cells may also flow through the pores to non-specific surfaces of the membrane material for attachment. These factors all affect the quantitative requirements of the experiment, increase cell loss, and also increase the risk of experimental errors.

[0042] Because the inner chamber 2 is equipped with a fixing part 4 and a gel layer, this application can fix the membrane material, prevent cells from flowing to non-specific surfaces of the membrane material and attaching, reduce cell loss, and reduce the risk of experimental errors.

[0043] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of those different embodiments or examples.

[0044] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "a plurality of" means two or more, unless otherwise explicitly specified.

[0045] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in this application, and these should all be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A device for directional cell attachment of membrane material, characterized in that, include: An outer chamber, the top of which has an outer chamber opening; An inner chamber is nested inside an outer chamber. A diaphragm material is placed inside the inner chamber. The bottom of the inner chamber has a fixing part, through which the diaphragm material is fixed inside the inner chamber. The top of the inner chamber has an inner chamber opening, which is located inside the outer chamber opening. A top cover that fits onto the top of the outer chamber and is used to seal the opening of the outer chamber; The inner chamber includes a bottom plate and four side plates connected in sequence. The bottom of each of the four side plates is connected to the bottom plate, and the bottom of each of the four side plates forms a fixing part with the bottom of the bottom plate. The edge of the diaphragm material is placed inside the fixing part. The bottom of each of the four side plates is bent away from the center of the inner chamber relative to the top of the side plate, forming a curved part. There is an angled space between the curved part and the bottom plate, and the angled space forms the fixing part. The four side plates are each provided with a mesh structure with a mesh aperture of less than 5µm, and the interior of the inner chamber is connected to the interior of the outer chamber through the mesh structure; A magnetic propeller is fixed to the bottom of the outer chamber.

2. The cell-directed attachment membrane material device according to claim 1, characterized in that, A gel layer is laid at the bottom of the inner chamber, with a specific side of the membrane material facing upwards, and the other side of the membrane material opposite to the specific side adhering to the gel layer.

3. The cell-directed attachment membrane material device according to claim 1, characterized in that, The inner chamber has a handle at its top edge, which can be mounted on the top edge of the outer chamber.

Images