Adjustable cell scratch experiment plug-in

Abstract

The utility model discloses an adjustable cell scratch experiment plug-in, belongs to the technical field of experiment plug-ins, and solves the problems that glass is fragile in texture, so that the glass is extremely easy to damage in the assembly and disassembly processes of an isolation baffle, the isolation baffle cannot be reused, and the production cost is reduced. And secondly, the problem that high-throughput experiment operation cannot be carried out on the cell culture plate is solved. Comprising an outer ferrule. When the cell culture tray is used, the outer sleeve ring is placed in a culture hole of the cell culture tray, and the number of the isolation baffles is selected according to requirements. The connecting block is manually inserted between the top disc and the bottom disc, the embedded sliding block enters the annular groove and slides, and after the distance between the baffles is adjusted, the supporting piece and the baffles are plugged into the outer ferrule till the bottom face abuts against the bottom of the culture hole groove. The baffle plate embedding block is embedded into the embedding hole, the external expansion lower abutting piece is placed, and the abutting rod abuts against the inner ring of the outer ferrule. The pressing bolt is rotated, so that the abutting rod expands the outer sleeve ring, the supporting piece and the baffle are pressed, scratches are formed, and gaps are avoided.

Description

Technical Field

[0001] This utility model relates to the field of experimental plug-in technology, and in particular to an adjustable cell scratch experimental plug-in. Background Technology

[0002] The cell scratch assay is a commonly used in vitro experimental method for studying cell migration ability. In this experiment, cells are cultured until a monolayer completely covers the culture dish. Then, one or more parallel "wounds" are made in the cell layer using a pipette tip or scratching tool, creating a cell-free area. The culture dish is then returned to the incubator for further cultivation, and the process of cells migrating from the sides of the scratches towards the center to fill the gaps is observed and recorded. Images of the scratched area are typically taken at different time points, and changes in scratch width are compared to assess cell migration speed and ability. This experiment is relatively simple to perform, low in cost, and provides a direct visual representation of cell motility characteristics. It has wide applications in studying cell physiological functions, the effects of drugs on cell migration, and the invasiveness of tumor cells.

[0003] The traditional scratch assay involves artificially creating scratches on a fused monolayer of cells using a pipette tip. Cells at the scratch edges are then observed to gradually migrate into a cell-free blank area, allowing the "scratch" to heal, demonstrating the cells' migration ability. However, this traditional method requires manual scratch creation, which demands skill from the operator. Improper technique can lead to uneven distribution of the cell-free blank area, ultimately affecting the accuracy of the experimental results.

[0004] Therefore, existing technologies often employ methods such as a cell scratch assay culture dish with application number CN201922494256.3. The key technical features include a culture dish body, an outer ring, an outer isolation baffle, a snap-fit ​​structure, and a transparent top cover. An outer ring is fixed to the center of the culture dish body. Several outer isolation baffles are fixed to the outer wall surface of the outer ring. A perforated groove is formed in the middle of the outer wall surface of the outer ring. An inner ring is fitted inside the outer ring. Several inner isolation baffles are fixed to the outer side surface of the inner ring. A snap-fit ​​structure is fixed to the upper surface of the inner ring. The snap-fit ​​structure includes a base plate, side plates, and L-shaped plates. The base plate is fixed to the upper surface of the inner ring. Vertical side plates are installed on both sides of the base plate. At least two L-shaped plates are provided at the front edge of the base plate. A gap is formed between the L-shaped plates and the front sides of the side plates. The outer wall of the outer ring is tightly fitted with the L-shaped plates. The upper surface of the side plates is fixed to the upper surface of the inner ring.

[0005] The above method creates cell scratches in the culture dish using an isolation baffle. However, since all the structures in the above method are made of glass, the glass itself is relatively brittle. Therefore, it is very easy to damage the isolation baffle during the assembly and disassembly process, which makes it impossible to reuse it. Secondly, it cannot be used for high-throughput experiments like cell culture plates.

[0006] Therefore, an adjustable cell scratch assay plugin is proposed to solve or alleviate the above problems. Utility Model Content

[0007] The purpose of this invention is to address the shortcomings of existing technologies by proposing an adjustable cell scratch assay plug-in.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] An adjustable cell scratch assay insert includes an outer ring, a support member that can be disposed within the outer ring, a plurality of isolation baffles detachably connected between the outer ring and the support member, and an outwardly expanding lower abutment detachably connected within the outer ring and located above the support member. The outer diameter of the outwardly expanding lower abutment can be increased or decreased, and the outwardly expanding lower abutment can apply a downward force to the support member. The plurality of isolation baffles can be slidably disposed on the outer ring of the support member. The outer ring, support member, isolation baffles, and outwardly expanding lower abutment are all made of polypropylene.

[0010] Preferably, the outer ring of the outer sleeve has a plurality of holes penetrating its inner ring, and the side of the isolation baffle away from the support member is fixedly connected to an embedding block, and the top and bottom surfaces of the embedding block are provided with guide embedding surfaces.

[0011] Preferably, the plurality of the recessed holes include odd-numbered hole groups and even-numbered hole groups.

[0012] Preferably, the support includes a bottom disc, a top disc, and a connecting column fixedly connected between the two. The bottom disc and the top disc have a lower annular groove and an upper annular groove respectively on their opposite sides. A connecting block is fixedly connected to the side of the isolation baffle away from the outer ring. An embedded slider is fixedly connected to the top and bottom surfaces of the connecting block. Guide surfaces are provided at both ends of the embedded slider along the radial direction of the isolation baffle support. The embedded sliders on both sides can be embedded into the upper annular groove and the lower annular groove and slide. The bottom surface of the isolation baffle is flush with the bottom surface of the bottom disc.

[0013] Preferably, the number of the isolation baffles is at least one.

[0014] Preferably, the isolation baffle has a through hole extending circumferentially along the outer ring.

[0015] Preferably, the outwardly expanding lower abutment includes an annular ring, a pressing rod, and a clamping bolt. The inner ring of the annular ring has a threaded hole, and the outer ring of the annular ring has a through groove communicating with the threaded hole. One end of the pressing rod is slidably connected in the through groove, and the end of the pressing rod that passes through the through groove to the threaded hole has a guide slope. The clamping bolt is threadedly connected in the threaded hole.

[0016] This utility model has the following beneficial effects:

[0017] In use, the outer ring is placed into the culture well of the cell culture tray, and the number of isolation baffles is selected according to requirements. The connecting block is manually inserted between the top and bottom discs, the sliding block is inserted into the annular groove and slids, and after adjusting the baffle spacing, the support and baffles are inserted into the outer ring until the bottom surface contacts the bottom of the culture well. The baffle insert is inserted into the recessed hole, the expanding lower abutment is placed in, and the clamping rod abuts against the inner ring of the outer ring. Rotating the clamping bolt expands the outer ring, pressing the support and baffles together, creating scratches to prevent gaps. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the outer ring structure in this utility model;

[0021] Figure 3 This is a schematic diagram of the structure of the support member and the isolation baffle in this utility model;

[0022] Figure 4 This is an exploded view of the support member and the isolation baffle in this utility model;

[0023] Figure 5 This is a schematic diagram of the structure of the outwardly expanding lower abutment in this utility model;

[0024] Figure 6 This is an exploded view of the outer expansion lower abutment component in this utility model.

[0025] 1. Outer ring; 2. Embedded hole; 3. Bottom disc; 4. Connecting post; 5. Top disc; 6. Lower annular groove; 7. Connecting block; 8. Embedded slider; 9. Isolation baffle; 10. Embedded block; 11. Through hole; 12. Annular ring; 13. Through groove; 14. Threaded hole; 15. Clamping bolt; 16. Clamping rod; 17. Guide slope. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0027] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0028] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0029] In the description of this utility model, it should be understood that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use, or the orientation or positional relationship commonly understood by those skilled in the art. They are only used to facilitate the description of this utility model and to simplify the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0030] Furthermore, the terms "first," "second," and "third" are used only to distinguish descriptions and should not be interpreted as indicating or implying relative importance.

[0031] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0032] An adjustable cell scratch assay plugin, such as Figures 1 to 6 As shown, it includes an outer ring 1, a support member that can be disposed within the outer ring 1, several isolation baffles 9 that are detachably connected between the outer ring 1 and the support member, and an outwardly expanding lower abutment that is detachably connected within the outer ring 1 and located above the support member. The outer diameter of the outwardly expanding lower abutment can be increased or decreased, and the outwardly expanding lower abutment can apply a downward force to the support member. The several isolation baffles 9 can be slidably disposed on the outer ring of the support member. The outer ring 1, the support member, the isolation baffles 9, and the outwardly expanding lower abutment are all made of polypropylene.

[0033] The outer ring 1 has several holes 2 that penetrate its inner ring. The side of the isolation baffle 9 away from the support is fixedly connected to an insert block 10. The top and bottom surfaces of the insert block 10 are provided with guide inserts. The holes 2 include odd-numbered hole groups and even-numbered hole groups.

[0034] The support includes a bottom disc 3, a top disc 5, and a connecting post 4 fixedly connected between the two. The bottom disc 3 and the top disc 5 have a lower annular groove 6 and an upper annular groove respectively on their opposite sides. A connecting block 7 is fixedly connected to the side of the isolation baffle 9 away from the outer ring 1. An embedded slider 8 is fixedly connected to the top and bottom surfaces of the connecting block 7. The embedded slider 8 has guide surfaces at both ends along the radial direction of the support of the isolation baffle 9. The embedded sliders 8 on both sides can be embedded in the upper annular groove and the lower annular groove 6 and slide. The bottom surface of the isolation baffle 9 is flush with the bottom surface of the bottom disc 3. There is at least one isolation baffle 9. A through hole 11 is opened on the isolation baffle 9 that runs through the circumference of the outer ring 1.

[0035] Preferably, the outwardly expanding lower abutment includes an annular ring 12, a pressing rod 16, and a clamping bolt 15. The inner ring of the annular ring 12 has a threaded hole 14, and the outer ring of the annular ring 12 has a through groove 13 communicating with the threaded hole 14. One end of the pressing rod 16 is slidably connected in the through groove 13, and the end of the pressing rod 16 that passes through the through groove 13 to the threaded hole 14 has a guide slope 17. The clamping bolt is threadedly connected in the threaded hole 14.

[0036] In practical application, this invention allows the outer ring 1 of the insert to be placed into the culture well of a cell culture tray. Then, based on the operator's needs, the number of sections to be divided into by scratches within a single culture well is determined, and the number of isolation baffles 9 is selected accordingly. The operator then manually aligns the connecting block 7 between the top disc 5 and the bottom disc 3, inserting the connecting block 7 between them. Simultaneously, the guide surface on the embedded slider 8 provides a guiding effect, allowing the embedded slider 8 to enter the upper and lower annular grooves 6 for sliding. The spacing between the isolation baffles 9 can be adjusted. Finally, the support and isolation baffles 9 can be inserted into the outer ring 1 until the bottom surfaces of the bottom disc 3 and the isolation baffles 9 are flush with the culture well. After the bottom of the hole is in contact with the support, the insert block 10 on the isolation baffle 9 needs to be inserted into the hole 2. The number of isolation baffles 9 determines whether to insert it into the odd or even number of hole groups. Finally, the outwardly expanding lower abutment is placed into the outer ring 1 and positioned above the support, so that the end of the abutment rod 16 can abut against the inner ring of the outer ring 1. Then, the clamping bolt 15 is rotated so that the clamping bolt 15 can apply a component force along its length direction to the clamping rod 16 under the guidance of the guide slope 17. This allows the clamping rod 16 to apply an outward expanding force to the outer ring 1, so that the outer ring 1 abuts against the inner ring of the culture hole. The stud of the compression clamping bolt 15 can press the support and the isolation baffle 9 together, so that the isolation baffle 9 can form scratches on the bottom of the culture hole, avoiding gaps between the isolation baffle 9 and the bottom of the culture hole.

[0037] If disassembly is required, simply rotate the clamping bolt 15 in the opposite direction to disengage the outer lower abutment from the outer ring 1, and then insert your fingers into the through hole in the isolation baffle 9 to pull the isolation baffle 9 and the support to separate them from the outer ring 1.

[0038] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. An adjustable cell scratch assay plug-in, characterized in that, The application relates to a supporting device for a centrifugal fan, which comprises a sleeve ring (1), a supporting piece arranged in the sleeve ring (1), a plurality of isolation baffles (9) detachably connected between the sleeve ring (1) and the supporting piece, and an outwardly expanded lower abutting piece detachably connected in the sleeve ring (1) and above the supporting piece, wherein the outer diameter of the outwardly expanded lower abutting piece can be increased or decreased, the outwardly expanded lower abutting piece can exert a downward force on the supporting piece, the plurality of isolation baffles (9) are arranged in a sliding mode outside the sleeve ring (1), and the sleeve ring (1), the supporting piece, the isolation baffles (9) and the outwardly expanded lower abutting piece are all made of polypropylene.

2. The adjustable cell scratch experiment insert of claim 1, wherein, The sleeve ring (1) is provided with a plurality of embedding holes (2) penetrating the inner circle of the sleeve ring (1), the isolation baffles (9) are fixedly connected with embedding blocks (10) on the side away from the supporting piece, and the top and bottom surfaces of the embedding blocks (10) are provided with guiding embedding surfaces.

3. The adjustable cell scratch experiment insert of claim 2, wherein, The plurality of embedding holes (2) comprise an odd hole group and an even hole group.

4. The adjustable cell scratch experiment insert of claim 1, wherein, The supporting piece comprises a bottom disc (3), a top disc (5) and a connecting column (4) fixedly connected between the bottom disc (3) and the top disc (5), the bottom disc (3) and the top disc (5) are respectively provided with a lower annular groove (6) and an upper annular groove on the side facing each other, the isolation baffles (9) are fixedly connected with connecting blocks (7) on the side away from the sleeve ring (1), the top and bottom surfaces of the connecting blocks (7) are fixedly connected with embedding sliding blocks (8), the embedding sliding blocks (8) are provided with guiding surfaces on both ends in the radial direction of the supporting piece, the embedding sliding blocks (8) on both sides can be embedded in the upper annular groove and the lower annular groove (6) and slide, and the bottom surface of the isolation baffles (9) is arranged in a flush mode with the bottom surface of the bottom disc (3).

5. The adjustable cell scratch experiment insert of claim 1, wherein, The number of the isolation baffles (9) is at least one.

6. The adjustable cell scratch experiment insert of claim 1, wherein, The isolation baffles (9) are provided with through holes (11) penetrating in the circumferential direction of the sleeve ring (1).

7. The adjustable cell scratch experiment insert of claim 1, wherein, The outwardly expanded lower abutting piece comprises a ring-shaped ring (12), an abutting rod (16) and a compression bolt (15), the inner circle of the ring-shaped ring (12) is provided with a threaded hole (14), the outer circle of the ring-shaped ring (12) is provided with a through groove (13) in communication with the threaded hole (14), one end of the abutting rod (16) is slidingly connected in the through groove (13), one end of the abutting rod (16) penetrating the through groove (13) into the threaded hole (14) is provided with a guiding inclined surface (17), and the compression bolt (15) is screw-connected in the threaded hole (14).

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