Detection kit for detecting content of lobetyolin in traditional Chinese medicinal materials

Abstract

The utility model relates to the technical field of detection kits, and discloses a detection kit for detecting the content of lobetyolin in traditional Chinese medicinal materials. The upper surface of the outer frame supporting frame is connected with a detachable elisa plate coated with lobetyolin antigen or a detachable elisa plate coated with goat-anti-mouse secondary antibody, one side of the outer frame supporting frame is provided with a placing frame, and the side edge of the placing frame is connected with the inner side of the detection reagent inner box; an enzyme marker placing tube, an anti-lobetyolin monoclonal antibody diluent placing tube, a developing solution placing tube, a stop solution placing tube, a sample diluent placing tube, six lobetyolin standard solution placing tubes with different concentrations and washing concentrated solution placing tubes are arranged on the placing rack. According to the detection kit for detecting the content of lobetyolin in the traditional Chinese medicinal materials, manual shaking is reduced, meanwhile, a plurality of sample test tubes can be placed in the test tube placing box at the same time, and the shaking efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of detection reagent inner box technology, specifically a detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicine. Background Technology

[0002] Codonopsis pilosula is the dried root of Codonopsis pilosula, Codonopsis lanceolata, or Codonopsis chuanxiong, all belonging to the Campanulaceae family. It has a sweet taste and neutral properties, and functions to invigorate the spleen and lungs, replenish qi, quench thirst, nourish blood, and promote the production of body fluids. As a traditional Chinese medicine, Codonopsis pilosula contains codonopsis glycosides, which are important active ingredients. Codonopsis glycosides have a good protective effect against gastric mucosal damage caused by ethanol. Before use, the content of codonopsis glycosides needs to be tested to ensure the medicinal properties of Codonopsis pilosula. A test kit is required for this test.

[0003] First, grind the sample into powder and place it in a test tube. Then, drop the test solution into the test tube and shake it manually to mix it evenly. After sealing the test tube, insert it into the test reagent box. By detecting the glycyrrhizic acid content of the Chinese medicinal material in different solutions, accurate data can be obtained.

[0004] When testing a small batch, shaking the sample manually can help to even it out. However, when the required sample size is large, manual shaking is time-consuming and labor-intensive, affecting the testing progress.

[0005] Currently, the content of codonopsis glycosides in Codonopsis pilosula and its products is mostly determined by high performance liquid chromatography (HPLC) and near-infrared spectroscopy. Although this method has high specificity and sensitivity, the sample pretreatment is cumbersome, the requirements for instruments and equipment are high, the detection time is long, and it is not suitable for rapid analysis and detection of large batches of samples.

[0006] Therefore, it is necessary to propose a detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials. Utility Model Content

[0007] To address the shortcomings of existing technologies, this invention provides a detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials. This kit features the advantage of using a mechanical structure to achieve uniform shaking of the test tube sample, reducing manual intervention in shaking and improving detection speed, thus solving the problems mentioned in the background technology.

[0008] This utility model provides the following technical solution: a detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials, comprising an inner detection kit and an outer detection kit:

[0009] The outer box of the test reagent is provided with an inner box of test reagents. A side connecting plate is attached to one side of the inner box of the test reagents, and a top block is fixedly connected to the upper end of the side connecting plate.

[0010] The top block has a slot on its upper surface, and slot holes are formed at both ends of the slot. A connecting shaft is movably connected inside the slot holes. A semi-circular protrusion is fixedly connected to the outer surface of the connecting shaft. One side of the semi-circular protrusion fits against the inner wall of the slot. A rotating frame with a shaft is rotatably connected to the side of the slot. A motor is installed on the outside of the top block. The output shaft of the motor passes through the inside of the slot and is connected to the rotating frame with a shaft. A test tube placement box is connected to the opposite end of the connecting shaft.

[0011] Preferably, side plates are fixedly connected to both sides of the side connecting plate, and bolt holes are provided on the side plates.

[0012] Preferably, the blade end of the rotating frame with shaft is in contact with the upper surface of the semi-circular protrusion, and the two sides of the test tube placement box are connected to the opposite ends of the connecting shaft by bolts.

[0013] Preferably, springs are fixedly connected to both ends of the upper surface of the semi-circular protrusion, and the other end of the spring is fixedly connected to the top of the inside of the groove.

[0014] Preferably, a sponge block is connected to the bottom of the test tube placement box, and an elastic covering cloth is connected to the upper surface of the test tube placement box by adhesive or binding.

[0015] Preferably, both the elastic covering fabric and the sponge block have placement grooves.

[0016] Preferably, one end of the bolt hole extends into the inner side of the test reagent box, and a bolt is connected inside the bolt hole.

[0017] Preferably, an outer frame support is installed on one side of the inner side of the outer box of the test reagent. The upper surface of the outer frame support is connected to an enzyme-labeled plate coated with codonopsis glycoside antigen or an enzyme-labeled plate coated with goat anti-mouse secondary antibody. A placement rack is provided on one side of the outer frame support. The side of the placement rack is connected to the inner side of the inner box of the test reagent. The placement rack is provided with enzyme label placement tubes, anti-codonopsis glycoside monoclonal antibody dilution solution placement tubes, colorimetric solution placement tubes, stop solution placement tubes, sample dilution solution placement tubes, six tubes of codonopsis glycoside standard solutions of different concentrations, and washing concentrate placement tubes.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] This reagent kit for detecting the content of codonopsis glycosides in traditional Chinese medicine involves placing the required sample tubes into a tube holder after shaking the sample in the test tubes. A motor drives a rotating frame with a shaft to rotate. The test tube holder is connected to a connecting shaft on both sides, and the outer surface of the connecting shaft has a center-downward-facing semi-circular protrusion. The connecting shaft is also connected to slots on both sides of a top block. The upper surface of the semi-circular protrusion is connected to the top of the slot via a spring. When the rotating frame rotates, its blades indirectly push the upper surface of the semi-circular protrusion, causing it to sway left and right around the axis of the connecting shaft. Simultaneously, the springs cause a slight up-and-down sway, thus mixing the sample and reagents inside the test tubes evenly. This mechanical design achieves uniform mixing of the test tube samples, enabling effective detection of codonopsis glycoside content and reducing manual shaking. Furthermore, the test tube holder can hold multiple sample tubes simultaneously, improving shaking efficiency. Attached Figure Description

[0020] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the overall structure of the device of this utility model;

[0022] Figure 2 This is a schematic diagram of the internal structure of the outer box of the test reagent of this utility model;

[0023] Figure 3 This is a schematic diagram of the cross-sectional structure of the top block of this utility model;

[0024] Figure 4 This utility model Figure 3 Partial structural diagram;

[0025] Figure 5 This utility model Figure 2 Partial structural diagram;

[0026] Figure 6 This is a schematic diagram of the internal structure of the test reagent box of this utility model.

[0027] The attached diagram lists the components represented by each number as follows:

[0028] 1. Inner box of test reagents;

[0029] 2. Side connecting plate; 210, Bolt 1; 220, Side plate; 230, Bolt hole;

[0030] 3. Top block; 310. Groove; 320. Test tube holder; 321. Sponge block; 322. Elastic covering cloth; 323. Placement groove; 330. Connecting shaft; 340. Semi-circular protrusion; 350. Rotating frame with shaft; 360. Slot; 370. Spring; 380. Two bolts; 390. Motor;

[0031] 4. Test reagent outer box; 410. Outer frame support frame; 420. Placement rack; 430. Enzyme label placement tube; 440. Anti-codonopsis glycoside monoclonal antibody dilution solution placement tube; 450. Colorimetric solution placement tube; 460. Stop solution placement tube; 470. Codonopsis glycoside standard solution placement tube; 480. Washing buffer placement tube; 490. Enzyme-labeled plate; 491. Sample dilution solution placement tube. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0033] Please see Figure 1 , Figure 2 , Figure 3 and Figure 4 A test kit for detecting the content of codonopsis glycosides in traditional Chinese medicine includes an inner test kit 1 and an outer test kit 4.

[0034] The outer box 4 of the test reagent is equipped with an inner box 1 of the test reagent. A side connecting plate 2 is attached to one side of the inner box 1 of the test reagent, and a top block 3 is fixedly connected to the upper end of the side connecting plate 2.

[0035] The top block 3 has a slot 310 on its upper surface. Both ends of the slot 310 have holes 360. A connecting shaft 330 is movably connected inside the holes 360. A semi-circular protrusion 340 is fixedly connected to the outer surface of the connecting shaft 330. One side of the semi-circular protrusion 340 is in contact with the inner wall of the slot 310. A rotating frame 350 with a shaft is rotatably connected to the side of the slot 310. A motor 390 is installed on the outside of the top block 3. The output shaft of the motor 390 passes through the inside of the slot 310 and is connected to the rotating frame 350 with a shaft. A test tube placement box 320 is connected to the opposite end of the connecting shaft 330. Springs 370 are fixedly connected to both ends of the upper surface of the semi-circular protrusion 340. The other end of the springs 370 is fixedly connected to the top of the inside of the slot 310.

[0036] When mixing the sample in the test tube, the required sample test tube is placed inside the test tube placement box 320. The motor 390 drives the rotating frame 350 with shaft to rotate. Since the two sides of the test tube placement box 320 are connected to the connecting shaft 330, and the outer surface of the connecting shaft 330 is connected to the center downward semi-circular protrusion 340, the connecting shaft 330 is connected to the slots 360 opened on both sides of the top block 3. At the same time, the upper surface of the semi-circular protrusion 340 is connected to the top of the slot 310 through the spring 370. When the rotating frame 350 with shaft rotates, its blades indirectly push the upper surface of the semi-circular protrusion 340, so that the semi-circular protrusion 340 can swing left and right around the axis of the connecting shaft 330. At the same time, under the action of the spring 370, it can swing slightly up and down, thereby mixing the sample and reagent inside the test tube evenly.

[0037] As a preferred technical solution of this utility model, side plates 220 are fixedly connected to both sides of the side connecting plate 2. Bolt holes 230 are opened on the side plates 220. One end of the bolt hole 230 extends into the inner side of the test reagent inner box 1. A bolt 210 is connected inside the bolt hole 230.

[0038] When shaking, one side of the side connecting plate 2 is attached to the side of the test reagent inner box 1. At this time, the bolt hole 230 on the side plate 220 coincides with the bolt hole 230 on the test reagent inner box 1. By connecting the bolt 210 into the bolt hole 230, the side connecting plate 2 can be tightly attached to one side of the test reagent inner box 1, ensuring the stability of shaking.

[0039] As a preferred technical solution of this utility model, the blade end of the rotating frame 350 with shaft is attached to the upper surface of the semi-circular protrusion 340, and the two sides of the test tube placement box 320 are connected to the opposite ends of the connecting shaft 330 by bolts 380.

[0040] The test tube placement box 320 and the connecting shaft 330 are connected by bolt 380, which facilitates the disassembly of the test tube placement box 320 and the loading of test tube samples.

[0041] As a preferred technical solution of this utility model, a sponge block 321 is connected to the bottom of the test tube placement box 320, and an elastic covering cloth 322 is connected to the upper surface of the test tube placement box 320 by means of adhesive or binding. Placement grooves 323 are provided on both the elastic covering cloth 322 and the sponge block 321.

[0042] As a preferred technical solution of this utility model, an outer frame support frame 410 is installed on one side of the inner side of the outer box 4 of the test reagent. An enzyme-labeled plate coated with codonopsis glycoside antigen or an enzyme-labeled plate coated with goat anti-mouse secondary antibody 490 is connected to the upper surface of the outer frame support frame 410. A placement rack 420 is provided on one side of the outer frame support frame 410. The side of the placement rack 420 is connected to the inner side of the inner box 1 of the test reagent. An enzyme label placement tube 430, an anti-codonopsis glycoside monoclonal antibody dilution solution placement tube 440, a colorimetric solution placement tube 450, a stop solution placement tube 460, a sample dilution solution placement tube 491, a six-tube codonopsis glycoside standard solution placement tube 470 of different concentrations, and a washing concentrate placement tube 480 are provided on the placement rack 420.

[0043] The ELISA plate 490 has 8 reaction wells and 12 strips evenly distributed on the outer support frame 410. The 12 reagent bottles consist of 6 bottles of different concentrations: standard solutions of different concentrations are in brown glass bottles with black caps, each with a capacity of 2 ml / bottle, and are all transparent and clear liquids; enzyme-labeled solutions are in white translucent PE plastic bottles with blue caps, each with a capacity of 10 ml / bottle, and are green liquids; anti-Codonopsis pilosula monoclonal antibody diluent is in white translucent PE plastic bottles with green caps, each with a capacity of 10 ml / bottle, and is orange-red liquid; chromogenic solution is in brown PE plastic bottles, each with a capacity of 15 ml / bottle, and is a transparent and clear liquid; stop solution is in translucent white PE plastic bottles with yellow caps, each with a capacity of 15 ml / bottle, and is a transparent and clear liquid; sample diluent is in translucent white PE plastic bottles with white caps, each with a capacity of 50 ml / bottle, and is a transparent and clear liquid; wash concentrate is in translucent white PE plastic bottles with white caps, each with a capacity of 50 ml / bottle, and is a transparent and clear liquid.

[0044] By preparing specific monoclonal antibodies against small molecule compounds of traditional Chinese medicine, a simple, rapid, and sensitive immunoassay method for traditional Chinese medicine can be established. Among the immunoassay methods, ELISA is the most commonly used. ELISA has the characteristics of high specificity, simple operation, high sensitivity, and easy automation. It can be used not only for antibody determination but also for antigen determination in samples. Its basic principle is as follows: (1) Antigens or antibodies are physically adsorbed on the surface of a solid-phase carrier (ELISA plate) and maintain their immunological activity; (2) Antigens or antibodies bind to enzymes to form enzyme conjugates and maintain immunological and enzyme activity; (3) Enzyme conjugates bind to the corresponding antigens or antibodies, and the addition of substrates can cause a color reaction. Since the intensity of the color of the reactants is directly proportional to the amount of the corresponding antigens or antibodies in the sample, the test results can be displayed according to the degree of substrate color development.

[0045] The detection method is as follows:

[0046] (1) Preparation of cleaning solution: Dilute the 10-fold concentrated cleaning solution with deionized water at a volume ratio of 1:9 according to the required amount (e.g., take 10 mL of 10-fold concentrated cleaning solution and add it to 90 mL of distilled water). Mix well and set aside for use.

[0047] (2) Take out the required number of enzyme-labeled plates 490 and insert them into the outer frame support frame 410. Record the position of each standard and sample solution. To reduce the fluctuation of the detection value, perform a two-well parallel experiment.

[0048] (3) Add 50 μL of standard solution / sample solution to the corresponding microwell, then add 50 μL of enzyme label / well and 50 μL of anti-codonopsis glycoside monoclonal antibody working solution / well. Gently shake for 5 seconds to mix, cover the plate and place in a 25°C dark environment for 15 min to react.

[0049] (4) Remove the cover film, shake off the liquid in the hole, add 300μL of cleaning solution per hole, wash 4 times, pour out the cleaning solution, and pat dry with absorbent paper.

[0050] (5) Add 100 μL of substrate colorimetric solution per well, mix well, cover the plate and incubate in a dark environment at 25°C for 15 min.

[0051] (6) Add 100 μL of stop solution per well, set the microplate reader to 450 nm, and measure the OD value.

[0052] In the description of this utility model, it should be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "other end", "upper", "side", "top", "inner", "front", "center", "both ends", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0053] In this utility model, unless otherwise explicitly specified and limited, the terms "installation", "setting", "connection", "fixing", "screw connection", etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal connection of two components or the interaction between two components. Unless otherwise explicitly limited, those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0054] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials, comprising an inner detection kit (1) and an outer detection kit (4), characterized in that: The outer box (4) of the test reagent is provided with an inner box (1) of the test reagent. A side connecting plate (2) is attached to one side of the inner box (1), and a top block (3) is fixedly connected to the upper end of the side connecting plate (2). The top block (3) has a slot (310) on its upper surface. Both ends of the slot (310) have slot holes (360). A connecting shaft (330) is movably connected inside the slot hole (360). A semi-circular protrusion (340) is fixedly connected to the outer surface of the connecting shaft (330). One side of the semi-circular protrusion (340) is in contact with the inner wall of the slot (310). A rotating frame (350) with a shaft is rotatably connected to the side of the slot (310). A motor (390) is installed on the outside of the top block (3). The output shaft of the motor (390) passes through the inside of the slot (310) and is connected to the rotating frame (350) with a shaft. A test tube placement box (320) is connected to the opposite end of the connecting shaft (330).

2. The inner box of the detection reagent for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials according to claim 1, characterized in that: Both sides of the side connecting plate (2) are fixedly connected with side plates (220), and bolt holes (230) are provided on the side plates (220).

3. The detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials according to claim 1, characterized in that: The blade end of the rotating frame (350) with shaft is attached to the upper surface of the semi-circular protrusion (340), and the two sides of the test tube placement box (320) are connected to the opposite ends of the connecting shaft (330) by bolts two (380).

4. The detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials according to claim 1, characterized in that: Both ends of the upper surface of the semi-circular protrusion (340) are fixedly connected to springs (370), and the other end of the springs (370) is fixedly connected to the top of the inside of the slot (310).

5. A detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials according to claim 1, characterized in that: The bottom of the test tube placement box (320) is connected to a sponge block (321), and the upper surface of the test tube placement box (320) is connected to an elastic covering cloth (322) by adhesive or binding.

6. The detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials according to claim 5, characterized in that: Both the elastic covering fabric (322) and the sponge block (321) are provided with placement grooves (323).

7. A detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials according to claim 2, characterized in that: One end of the bolt hole (230) extends into the inner side of the test reagent inner box (1), and a bolt (210) is connected inside the bolt hole (230).

8. A detection kit for detecting the content of codonopsis glycosides in traditional Chinese medicinal materials according to claim 2, characterized in that: An outer frame support (410) is installed on one side of the inner side of the outer box (4) of the test reagent. An enzyme-labeled plate coated with codonopsis glycoside antigen or an enzyme-labeled plate coated with goat anti-mouse secondary antibody (490) is connected to the upper surface of the outer frame support (410). A placement rack (420) is provided on one side of the outer frame support (410). The side of the placement rack (420) is connected to the inner side of the inner box (1) of the test reagent. An enzyme label placement tube (430), an anti-codonopsis glycoside monoclonal antibody dilution solution placement tube (440), a colorimetric solution placement tube (450), a stop solution placement tube (460), a sample dilution solution placement tube (491), six tubes of codonopsis glycoside standard solutions of different concentrations (470), and a washing concentrate placement tube (480) are provided on the placement rack (420).

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