Preparation device of glucose stevioside

Abstract

The utility model discloses a preparation device of glucose stevioside, and relates to the technical field of glucose stevioside production, an inlet of a conversion tank is respectively communicated with a raw material tank, an auxiliary material tank and a cyclodextrin glucoside transferase tank, an outlet of the conversion tank is communicated with a hydrolysis tank, an outlet of the hydrolysis tank is communicated with a reaction tank, and the reaction tank is communicated with a reaction tank. The inlet of the reaction tank is respectively communicated with a sucrose tank, a sucrose synthase tank, a uridine diphosphate tank, a first UGT glycosyltransferase tank, a second UGT glycosyltransferase tank and an alkali liquor tank; an outlet of the reaction tank is communicated with a first macroporous adsorption resin column, an inlet of the first macroporous adsorption resin column is communicated with a first ethanol tank, an outlet of the first macroporous adsorption resin column is communicated with a first concentration tank, and a lower outlet of the first concentration tank is communicated with a first dryer; and an outlet of the first dryer is communicated with a first glucosyl stevioside tank. And the residual substrate is converted into stevioside with relatively better taste, so that the taste and quality of the product are improved.

Description

An apparatus for preparing glucose steviol glycosides Technical Field

[0001] This utility model relates to the field of glucose steviol glycoside production technology, specifically to a glucose steviol glycoside preparation apparatus. Background Technology

[0002] Currently, the production of glucosyl steviol glycosides mainly involves glucoside A or steviol glycosides as raw materials, which are then glucosylated by cyclodextrin transferase. However, due to the high conversion efficiency of beta-cyclodextrin glycosidase and the high content of monoglycosides in the raw materials, unreacted glycosides exceed 4%, resulting in products that do not meet standards. Increasing the amount of dextrin added can, to some extent, increase the substrate conversion rate and reduce unreacted glycosides. However, this method requires a large amount of dextrin and is difficult to reduce unreacted glycosides to below the 4% limit. Excessive dextrin also reduces the sweetness and taste of the product. While compounding steviol glycosides can dilute unreacted glycosides, the addition of other steviol glycosides can significantly alter the product's properties and result in products with substandard total glycoside content, also failing to meet quality standards. Resin separation is another option, but this method suffers from low yield, increased waste, and a high degree of glycosylation, which also significantly alters the product's properties, limiting the application of glucosyl steviol glycoside products using these raw materials as substrates. Summary of the Invention

[0003] The technical problem to be solved by this utility model is to provide a device for preparing glucose steviol glycosides, which reduces the content of unreacted glycosides in the product and improves the taste and quality of the product, in order to address the shortcomings of the existing technology.

[0004] To solve the above-mentioned technical problems, the technical solution of this utility model is as follows:

[0005] An apparatus for preparing glucosinolates includes a conversion tank. The inlet of the conversion tank is connected to a raw material tank, an excipient tank, and a cyclodextrin glucosyltransferase tank via pipelines. The outlet of the conversion tank is connected to a hydrolysis tank via pipelines. The inlet of the hydrolysis tank is connected to an α-amylase tank via pipelines. The outlet of the hydrolysis tank is connected to a reaction tank via pipelines. The inlet of the reaction tank is connected to a sucrose tank, a sucrose synthase tank, a uridine diphosphate tank, a first UGT glycosyltransferase tank, a second UGT glycosyltransferase tank, and an alkaline solution tank via pipelines.

[0006] The outlet of the reaction vessel is connected to a first macroporous adsorption resin column via a pipeline. The inlet of the first macroporous adsorption resin column is connected to a first ethanol tank via a pipeline. The outlet of the first macroporous adsorption resin column is connected to a first concentration tank via a pipeline. The lower outlet of the first concentration tank is connected to a first dryer via a pipeline. The outlet of the first dryer is connected to a first glucosylstevioside tank.

[0007] As an improved technical solution, the top outlet of the first concentration tank is connected to a first ethanol recovery tank via a pipeline.

[0008] As an improved technical solution, the inlet of the first macroporous adsorption resin column is connected to a first deionized water tank via a pipeline.

[0009] As an improved technical solution, the outlet of the first macroporous adsorption resin column is connected to a second macroporous adsorption resin column via a pipeline, the inlet of the second macroporous adsorption resin column is connected to a second ethanol tank, the outlet of the second macroporous adsorption resin column is connected to a second concentration tank via a pipeline, the lower outlet of the second concentration tank is connected to a second dryer via a pipeline, and the outlet of the second dryer is connected to a second glucosylstevioside tank.

[0010] As an improved technical solution, the top outlet of the second concentration tank is connected to a second ethanol recovery tank via a pipeline.

[0011] As an improved technical solution, the inlet of the second macroporous adsorption resin column is connected to a second deionized water tank via a pipeline.

[0012] Due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0013] This invention relates to a device for preparing glucosylstevioside, comprising a conversion tank. The inlet of the conversion tank is connected via pipes to a raw material tank, an excipient tank, and a cyclodextrin glucoside transferase tank. The outlet of the conversion tank is connected via pipes to a hydrolysis tank. The inlet of the hydrolysis tank is connected via pipes to an α-amylase tank. The outlet of the hydrolysis tank is connected via pipes to a reaction tank. The inlet of the reaction tank is connected via pipes to a sucrose tank, a sucrose synthase tank, a uridine diphosphate tank, a first UGT glycosyltransferase tank, a second UGT glycosyltransferase tank, and an alkaline solution tank. The outlet of the reaction tank is connected via pipes to a first macroporous adsorption resin column. The inlet of the first macroporous adsorption resin column is connected via pipes to a first ethanol tank. The outlet of the first macroporous adsorption resin column is connected via pipes to a first concentration tank. The lower outlet of the first concentration tank is connected via pipes to a first dryer. The outlet of the first dryer is connected to a first glucosylstevioside tank. Rebaudioside A or steviol glycoside is used as the raw material, beta-cyclodextrin as the excipient, and cyclodextrin glucoside transferase (…) is added. A 3.0L solution undergoes glycosylation to obtain a glucosylstevioside solution using rebaudioside A or steviol glycoside as a substrate. This solution is then hydrolyzed by α-amylase to yield low-glycosylated, long-chain glucosylstevioside. This is further reacted with sucrose, first UGT glycosyltransferase, second UGT glycosyltransferase, sucrose synthase, and uridine diphosphate glucose to convert unreacted rebaudioside A or steviol glycosides in the solution into rebaudioside M. The reaction solution is then fed into a first macroporous adsorption resin column to adsorb the relevant steviol glycosides while removing residual steviol glycosides. Dextrin and sucrose are concentrated and dried after ethanol analysis to obtain glucosylstevioside, which converts the residual substrate into steviol glycoside with a relatively better taste, thus improving the taste and quality of the product. In addition, the conversion effect is better when using a mixed enzyme of UGT91C1 (derived from the N4 protein encoded by the UGT91C1 mutant gene in Chinese patent CN119265152B) and UGT76G4 (derived from the protein encoded by the UGT76G4 gene in Chinese patent CN115094074B).

[0014] The top outlet of the first concentration tank of this invention is connected to a first ethanol recovery tank via a pipeline. This enables the recovery and reuse of ethanol, which not only reduces production costs and wastes ethanol, an organic solvent, but also conforms to the concept of green chemistry and reduces environmental pollution.

[0015] The inlet of the first macroporous adsorption resin column is connected to a first deionized water tank via a pipeline. The deionized water can be used to clean the resin column, remove residual impurities, ensure the stable adsorption performance of the macroporous adsorption resin column, extend its service life, and reduce equipment maintenance costs.

[0016] The outlet of the first macroporous adsorption resin column is connected to a second macroporous adsorption resin column via a pipeline. The inlet of the second macroporous adsorption resin column is connected to a second ethanol tank. The outlet of the second macroporous adsorption resin column is connected to a second concentration tank via a pipeline. The lower outlet of the second concentration tank is connected to a second dryer via a pipeline. The outlet of the second dryer is connected to a second glucosylstevioside tank. The second macroporous adsorption resin column is desorbed by ethanol. The desorbed solution is concentrated and dried to obtain the glucosylstevioside product. Through two adsorption and desorption processes, the product yield is improved and the waste of raw materials is reduced.

[0017] The top outlet of the second concentration tank is connected to a second ethanol recovery tank via a pipeline, enabling the recycling of ethanol again, further reducing production costs and environmental pollution.

[0018] The inlet of the second macroporous adsorption resin column is connected to a second deionized water tank via a pipeline, which can be used to clean and maintain the second macroporous adsorption resin column, ensuring its continuous and efficient adsorption performance and guaranteeing the stable operation of the entire preparation process. Attached Figure Description

[0019] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0020] Figure 1 is a structural schematic diagram of an embodiment of the present utility model;

[0021] The tank consists of: 1. Conversion tank; 2. Raw material tank; 3. Auxiliary material tank; 4. Cyclodextrin glucoside transferase tank; 5. Hydrolysis tank; 6. α-amylase tank; 7. Reaction tank; 8. Sucrose tank; 9. Sucrose synthase tank; 10. Urate diphosphate tank; 11. First UGT glycosyltransferase tank; 12. Second UGT glycosyltransferase tank; 13. Alkali tank; 14. First macroporous adsorption resin column; 15. First ethanol tank; 16. First concentration tank; 17. First dryer; 18. First glucosylstevioside tank; 19. First ethanol recovery tank; 20. First deionized water tank; 21. Second macroporous adsorption resin column; 22. Second ethanol tank; 23. Second concentration tank; 24. Second dryer; 25. Second glucosylstevioside tank; 26. Second ethanol recovery tank; 27. Second deionized water tank. Detailed Implementation

[0022] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0023] As shown in Figure 1, an apparatus for preparing glucosinolates includes a conversion tank 1. The inlet of the conversion tank 1 is connected to a raw material tank 2, an excipient tank 3, and a cyclodextrin glucoside transferase tank 4 via pipes. The outlet of the conversion tank 1 is connected to a hydrolysis tank 5 via a pipe. The inlet of the hydrolysis tank 5 is connected to an α-amylase tank 6 via a pipe. The outlet of the hydrolysis tank 5 is connected to a reaction tank 7 via a pipe. The inlet of the reaction tank 7 is connected to a sucrose tank 8, a sucrose synthase tank 9, a uridine diphosphate tank 10, and a first UGT via pipes. The reaction vessel 7 comprises a glycosyltransferase tank 11, a second UGT glycosyltransferase tank 12, and an alkaline tank 13. The outlet of the reaction vessel 7 is connected via a pipe to a first macroporous adsorption resin column 14. The inlet of the first macroporous adsorption resin column 14 is connected via a pipe to a first ethanol tank 15. The outlet of the first macroporous adsorption resin column 14 is connected via a pipe to a first concentration tank 16. The lower outlet of the first concentration tank 16 is connected via a pipe to a first dryer 17. The outlet of the first dryer 17 is connected to a first glucosylstevioside tank 18. Rebaudioside A or steviol glycoside is used as the raw material, beta-cyclodextrin is used as the excipient, and cyclodextrin glucosyltransferase (Cyclodextrin Glucosyltransferase) is added. A 3.0L solution undergoes glycosylation to obtain a glucosylstevioside solution with rebaudioside A or steviol as the substrate. This solution is then hydrolyzed by α-amylase to yield low-glycosylated, long-chain glucosylstevioside. This is further reacted with sucrose, first UGT glycosyltransferase, second UGT glycosyltransferase, sucrose synthase, and uridine diphosphate glucose to convert unreacted rebaudioside A or steviol in the solution into rebaudioside M. The reaction solution is then fed into a macroporous adsorption resin column 14 to adsorb the relevant steviol glycosides while removing residual dextrin and sucrose. After ethanol elution, the solution is concentrated and dried to obtain glucosylstevioside, thus converting the residual substrate... The conversion into steviol glycosides, which have a relatively better taste, improves the product's flavor and quality. In addition, the mixed enzyme combination of UGT91C1 (derived from the N4 protein encoded by the UGT91C1 mutant gene in Chinese patent CN119265152B) and UGT76G4 (derived from the protein encoded by the UGT76G4 gene in Chinese patent CN115094074B), with an enzyme activity ratio of 1 to 1.5:1 for UGT91C1 to UGT76G4, and sucrose synthase derived from the sucrose synthase AtSuSy in Chinese patent CN119265152B, results in even better conversion.

[0024] The top outlet of the first concentration tank 16 is connected to the first ethanol recovery tank 19 via a pipeline. This enables the recycling and reuse of ethanol, which not only reduces production costs and wastes this organic solvent, but also conforms to the concept of green chemistry and reduces environmental pollution.

[0025] The inlet of the first macroporous adsorption resin column 14 is connected to a first deionized water tank 20 through a pipeline. The deionized water can be used to clean the resin column, remove residual impurities, ensure the stable adsorption performance of the macroporous adsorption resin column, extend its service life, and reduce equipment maintenance costs.

[0026] The outlet of the first macroporous adsorption resin column 14 is connected to a second macroporous adsorption resin column 21 via a pipeline. The inlet of the second macroporous adsorption resin column 21 is connected to a second ethanol tank 22. The outlet of the second macroporous adsorption resin column 21 is connected to a second concentration tank 23 via a pipeline. The lower outlet of the second concentration tank 23 is connected to a second dryer 24 via a pipeline. The outlet of the second dryer 24 is connected to a second glucosyl steviol glycoside tank 25. The second macroporous adsorption resin column 21 is desorbed by ethanol. The desorbed solution is concentrated and dried to obtain glucosyl steviol glycoside product II. Through two adsorption and desorption processes, the product yield is improved and the waste of raw materials is reduced.

[0027] The top outlet of the second concentration tank 23 is connected to the second ethanol recovery tank 26 via a pipeline, which enables the recovery and reuse of ethanol, further reducing production costs and environmental pollution.

[0028] The inlet of the second macroporous adsorption resin column 21 is connected to a second deionized water tank 27 via a pipeline, which can be used to clean and maintain the second macroporous adsorption resin column 21, ensuring its continuous and efficient adsorption performance and guaranteeing the stable operation of the entire preparation process.

[0029] It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that after reading the teachings of this invention, those skilled in the art can make various alterations or modifications to the invention, and these equivalent forms also fall within the scope defined by the appended claims.

Claims

1. An apparatus for preparing glucose steviol glycosides, characterized in that: The system includes a conversion tank, whose inlet is connected via pipes to a raw material tank, an excipient tank, and a cyclodextrin glucoside transferase tank. The outlet of the conversion tank is connected via pipes to a hydrolysis tank, whose inlet is connected via pipes to an α-amylase tank. The outlet of the hydrolysis tank is connected via pipes to a reaction tank, whose inlet is connected via pipes to a sucrose tank, a sucrose synthase tank, a uridine diphosphate tank, a first UGT glycosyltransferase tank, a second UGT glycosyltransferase tank, and an alkaline solution tank. The outlet of the reaction tank is connected via pipes to a first macroporous adsorption resin column, whose inlet is connected via pipes to a first ethanol tank. The outlet of the first macroporous adsorption resin column is connected via pipes to a first concentration tank, whose lower outlet is connected via pipes to a first dryer, and whose outlet is connected via pipes to a first glucosylstevioside tank.

2. The apparatus for preparing glucose steviol glycosides as described in claim 1, characterized in that: The top outlet of the first concentration tank is connected to a first ethanol recovery tank via a pipeline.

3. The apparatus for preparing glucose steviol glycosides as described in claim 1, characterized in that: The inlet of the first macroporous adsorption resin column is connected to a first deionized water tank via a pipe.

4. The apparatus for preparing glucose steviol glycosides as described in claim 1, characterized in that: The outlet of the first macroporous adsorption resin column is connected to a second macroporous adsorption resin column via a pipeline. The inlet of the second macroporous adsorption resin column is connected to a second ethanol tank. The outlet of the second macroporous adsorption resin column is connected to a second concentration tank via a pipeline. The lower outlet of the second concentration tank is connected to a second dryer via a pipeline. The outlet of the second dryer is connected to a second glucosylstevioside tank.

5. The apparatus for preparing glucose steviol glycosides as described in claim 4, characterized in that: The top outlet of the second concentration tank is connected to a second ethanol recovery tank via a pipeline.

6. The apparatus for preparing glucose steviol glycosides as described in claim 4, characterized in that: The inlet of the second macroporous adsorption resin column is connected to a second deionized water tank via a pipe.

Images