PCR (Polymerase Chain Reaction) reagent split charging box

Abstract

The utility model relates to a PCR (Polymerase Chain Reaction) reagent split charging box, which comprises a box body, the box body comprises a lower seat, a boss I and a boss II are arranged on the lower seat, the boss I and the boss II are arranged at an interval, and pipe holes which vertically penetrate into the lower seat are formed in the boss I and the boss II and are respectively a reagent pipe hole and a control pipe hole; and the cover body can be separated from the box body and comprises a first marking cover and a second marking cover, and the first marking cover and the second marking cover are arranged on the outer side of the first boss and the outer side of the second boss in a sleeving mode respectively. According to the PCR reagent split charging box, the double-boss physical isolation design is accompanied by the interval design, so that the reagent area and the contrast area are separated, the risk of cross contamination is reduced, the pipe holes ensure that all split charging pipes are vertically fixed, inclined liquid leakage is avoided, taking and using are convenient, the experiment convenience is improved, marking cavities in the first marking cover and the second marking cover can provide marking areas, and the marking areas can be conveniently used. The length and the width of the marking cover I are respectively greater than those of the marking cover II, so that the distinguishing performance is enhanced, and kit contrast can be distinguished.

Description

Technical Field

[0001] This utility model relates to the field of reagent kit technology, specifically a PCR reagent dispensing kit. Background Technology

[0002] Currently, most reagent manufacturers offer large-volume packaging, meaning that many reagent (PCR amplification reagent) manufacturers provide large-capacity packaging (enough for dozens of experiments). A single experiment cannot use up an entire bottle of reagent, so the reagent needs to be dissolved before use. Only a portion is used in each experiment, and the remaining portion needs to be repeatedly frozen and thawed (frozen storage, then dissolved before use). Reconstitution (repeated freezing and thawing) will significantly reduce the activity and stability of the reagent, affecting the accuracy and reliability of the experimental results.

[0003] To address the issue of repeated freeze-thaw cycles, the common practice in laboratories is to immediately aliquot the reagents into smaller portions after the initial thawing of the bulk packaging. These smaller aliquots are then stored in an empty compartment of the original kit provided by the manufacturer. However, the original kit is primarily designed to accommodate the bulk reagent tubes that come with it. These aliquots often cannot be held upright in the kit like the original tubes; they must be tilted or laid flat. This not only takes up inefficient space but, more importantly, the lid's seal may deteriorate when not upright, leading to reagent leakage, waste, contamination of the work surface, and even interference with other reagents. Furthermore, the kit usually includes positive controls for experimental quality control. If the positive controls are stored in the same open compartment as the laboratory-allocated test reagents, there is a risk of cross-contamination. If the positive control leaks, it can easily contaminate the adjacent aliquots, leading to false positive results in subsequent experiments. Therefore, a PCR reagent aliquot box is proposed. Utility Model Content

[0004] Based on the above description, this utility model provides a PCR reagent dispensing kit, which solves the technical problems pointed out in the background art.

[0005] The technical solution of this utility model to solve the above-mentioned technical problems is as follows: A PCR reagent dispensing box, comprising:

[0006] The box body includes a lower seat, on which a first boss and a second boss are provided. The first boss and the second boss are spaced apart. Vertical tube holes are opened on both the first boss and the second boss, which are respectively reagent tube holes and control tube holes.

[0007] The cover is detachable from the box body and includes a first marking cover and a second marking cover, which are respectively fitted onto the outside of the first and second protrusions.

[0008] Based on the above technical solution, the present invention can be further improved as follows.

[0009] Furthermore, the reagent tube has a total of ninety-six holes, arranged in eight rows and twelve columns.

[0010] Furthermore, there are a total of ten control tube holes, of which four are large holes and six are small holes, arranged in five rows and two columns.

[0011] Furthermore, the length and width of the first marking cover are greater than the length and width of the second marking cover, respectively, in order to distinguish them.

[0012] Furthermore, both the first and second marking covers are provided with marking cavities.

[0013] Furthermore, grooves are provided on both the front and rear sides of the first and second protrusions, and receiving cavities are provided on both the front and rear sides of the first and second marking covers. The PCR reagent dispensing box also includes two sets of locking structures. The locking structure includes a slider located in the receiving cavity. A limiting protrusion matching the groove is provided on the opposite side of each of the two sets of sliders. The locking structure also includes an adjusting transmission component for moving the two sets of limiting protrusions toward each other or away from each other.

[0014] Furthermore, both the first and second marking covers have a perforation at the center of their tops. The adjusting transmission component also includes a transmission shaft rotatably connected to the inner side of the corresponding perforation. A meshing wheel is fixed at the bottom of the transmission shaft, and transmission bars are provided on the left and right sides of the meshing wheel. The transmission bars are fixedly connected to the slide bars. Multiple meshing teeth are fixed on the side of the transmission bar near the meshing wheel to allow the transmission bar to mesh with the meshing wheel. The top of the transmission shaft extends through the perforation and is fixed with a rotating cap. The diameter of the rotating cap is larger than the diameter of the perforation.

[0015] Furthermore, the bottom of the rotating cap is provided with a mounting groove, and a spring sleeved on the outside of the drive shaft is provided in the mounting groove.

[0016] Furthermore, both the inner top walls of the first and second marking covers are provided with transmission grooves, which are connected to the corresponding two sets of receiving cavities. The transmission bar and meshing wheel in the locking structure are located in the corresponding transmission grooves. Steps are provided in the transmission grooves, and a panel is fixed to the steps by bolts. The transmission bar is slidably connected to the upper surface of the panel.

[0017] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0018] 1. This PCR reagent dispenser features a double-protrusion physical isolation design with a spacer to separate the reagent area from the control area, reducing the risk of cross-contamination. The tube openings ensure that all dispensers stand upright and are fixed in place, preventing tilting and leakage, and making them easy to use, thus increasing the convenience of experiments.

[0019] 2. The PCR reagent kit has labeling cavities on labeling cap one and labeling cap two, which can provide labeling areas. The length and width of labeling cap one are greater than the length and width of labeling cap two, respectively, to enhance the differentiation performance and distinguish the kit control. The locking structure design strengthens the stable connection between the cap and the kit body. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of a PCR reagent dispensing box according to an embodiment of the present invention.

[0021] Figure 2 for Figure 1 A schematic diagram of the structure after the middle box and the lid are separated;

[0022] Figure 3 This is a schematic diagram of the structure of a PCR reagent dispensing box according to Embodiment 2 of the present invention;

[0023] Figure 4 for Figure 3 A schematic diagram of the structure after the middle box and the lid are separated;

[0024] Figure 5 for Figure 3 A schematic diagram of the cross-sectional structure;

[0025] Figure 6 for Figure 5 A magnified view of a portion of region A in the middle;

[0026] Figure 7 for Figure 5 A magnified view of a portion of region B in the middle;

[0027] Figure 8 for Figure 3 Schematic diagram of the component structure of the middle cover;

[0028] Figure 9 for Figure 8 A cross-sectional view of the structure of the central marking cover;

[0029] Figure 10 This invention provides a schematic diagram of the locking structure in a PCR reagent dispenser.

[0030] Figure 11 for Figure 10 A magnified view of a portion of region C.

[0031] The attached diagram lists the components represented by each number as follows:

[0032] 1. Box body; 11. Lower seat; 12. Boss 1; 121. Reagent tube hole; 13. Boss 2; 131. Control tube hole; 2. Cover body; 21. Marking cover 1; 22. Marking cover 2; 23. Receiving cavity; 24. Transmission groove; 25. Panel; 3. Groove; 4. Locking structure; 41. Sliding bar; 42. Limiting protrusion; 43. Transmission shaft; 44. Engaging wheel; 45. Transmission bar; 46. Rotary cap; 47. Spring. Detailed Implementation

[0033] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.

[0034] Example 1

[0035] like Figure 1-2 As shown in this embodiment, a PCR reagent dispensing kit includes a box body 1 and a detachable cover 2.

[0036] The following will describe the box body 1 and the lid body 2, first as follows: Figure 2 As shown, the box body 1 consists of a lower base 11 and two independent protrusions integrally connected to it. The two independent protrusions are protrusion one 12 and protrusion two 13. Both protrusion one 12 and protrusion two 13 have vertically penetrating tube holes that extend into the lower base 11. Specifically, protrusion one 12 has 96 vertically penetrating reagent tube holes 121 arranged in an 8-row × 12-column matrix for upright placement of dispensed PCR reagent tubes and has a large capacity. Protrusion two 13 also has ten vertically penetrating control tube holes 131 arranged in a 5-row × 2-column matrix. Four of these are large holes for 1.5ml EP tubes or 1.5ml to 2ml plastic cryopreservation tubes, and six are small holes for 0.5ml EP tubes. This allows for the use of control products from different manufacturers.

[0037] It should be noted that protrusion 12 and protrusion 2 13 are spaced apart. This double protrusion physical isolation design, along with the spacing design, separates the reagent area from the control area, reducing the risk of cross-contamination. Meanwhile, the tube holes ensure that all dispensing tubes are fixed upright to avoid tilting and leakage.

[0038] The cover 2 includes two independent marking covers, namely marking cover one 21 and marking cover two 22. Marking cover one 21 and marking cover two 22 are respectively fitted on the outside of protrusion one 12 and protrusion two 13, forming a surround of protrusion one 12 and protrusion two 13.

[0039] It should be noted that both the first label 21 and the second label 22 are provided with labeling cavities, which can be sprayed, pasted or engraved with distinguishing marks to facilitate identification of the reagent types contained inside. Furthermore, the length and width of the first label 21 are greater than those of the second label 22, respectively, to enhance the distinguishing performance and facilitate differentiation.

[0040] Aliquoting procedure: Dissolve the bulk PCR reagent and aliquot it into 1-96 0.2mL centrifuge tubes. Insert the tubes upright into well 121 of the reagent tubes. After aliquoting the control, insert the control into the large and small wells of well 131 of the control tubes.

[0041] Example 2

[0042] To strengthen the secure connection between the lid 2 and the box 1, based on the above-mentioned technology, such as Figure 3-11 A PCR reagent dispensing box is also designed with two sets of locking structures 4, which correspond to the connection between the label cap 1 21 and the label cap 22 and the box body 1.

[0043] It should be noted that, in this design, if Figure 4 As shown, both the front and rear sides of boss 12 and boss 23 are provided with grooves 3, such as... Figure 6 As shown, both the front and rear sides of the first marking cover 21 and the second marking cover 22 are provided with receiving cavities 23.

[0044] As shown below, the locking structure 4 includes a slide bar 41 located in the receiving cavity 23. Each of the two sets of slide bars 41 is provided with a limiting protrusion 42 that matches the groove 3 on the opposite side. The locking structure 4 also includes an adjusting transmission component for moving the two sets of limiting protrusions 42 toward each other or away from each other.

[0045] Further explanation: both the top center of the first marking cover 21 and the second marking cover 22 are provided with through holes. The adjusting transmission component also includes a transmission shaft 43 rotatably connected to the inner side of the corresponding through hole. A meshing wheel 44 is fixed at the bottom of the transmission shaft 43. Transmission bars 45 are provided on the left and right sides of the meshing wheel 44. The transmission bars 45 are fixedly connected to the slide bar 41. Multiple meshing teeth are fixed on the side of the transmission bar 45 near the meshing wheel 44, so that the transmission bar 45 meshes with the meshing wheel 44. In this way, when the meshing wheel 44 rotates, the two transmission bars 45 in the locking structure 4 can move towards or away from each other. In addition, the top end of the transmission shaft 43 extends out of the through hole and is fixed with a swivel cap 46 for the operator to operate to adjust whether the meshing wheel 44 rotates or not. The diameter of the swivel cap 46 is larger than the diameter of the through hole to prevent it from passing through the through hole.

[0046] In a preferred embodiment, the bottom of the rotating cap 46 is provided with a mounting groove, and a spring 47 is provided in the mounting groove and sleeved on the outside of the drive shaft 43 to prevent the rotating cap 46 from rotating arbitrarily. That is, the preload of the spring 47 can prevent transportation vibration from causing unlocking.

[0047] In addition, to conceal the meshing wheel 44 and the transmission bar 45, the inner top walls of the first marking cover 21 and the second marking cover 22 are provided with transmission grooves 24. The transmission grooves 24 are connected to the corresponding two sets of receiving cavities 23. The transmission bar 45 and the meshing wheel 44 in the locking structure 4 are located in the corresponding transmission grooves 24. Steps are provided in the transmission grooves 24, and the panel 25 is fixed to the steps by bolts. The bolts are not shown in the figure. The transmission bar 45 is slidably connected to the upper surface of the panel 25.

[0048] The operation and transmission process of the locking structure 4 are as follows:

[0049] Locking: Rotate the cap 46 clockwise, the drive shaft 43 drives the meshing wheel 44 to rotate, the two sets of drive bars 45 move in opposite directions, the slide bar 41 pushes the limiting protrusion 42 into the groove 3, and the cover 2 is firmly connected to the box 1.

[0050] Unlock: Rotate the cap 46 counterclockwise, the transmission bar 45 moves in the opposite direction, the limiting protrusion 42 exits the groove 3, and the cover 2 can be separated.

[0051] The above are merely preferred embodiments of this utility model, but the scope of protection of this utility model is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in this utility model, based on the technical solution and inventive concept of this utility model, should be included within the scope of protection of this utility model.

Claims

1. A PCR reagent dispenser, characterized in that, include: The box body (1) includes a lower seat (11), on which a first boss (12) and a second boss (13) are provided. The first boss (12) and the second boss (13) are spaced apart. Both the first boss (12) and the second boss (13) have vertical tube holes that penetrate into the lower seat (11), which are respectively a reagent tube hole (121) and a control tube hole (131). The cover (2) is separable from the box (1) and includes a first marking cover (21) and a second marking cover (22), which are respectively fitted on the outside of the first boss (12) and the second boss (13).

2. The PCR reagent dispensing kit according to claim 1, characterized in that: There are a total of ninety-six reagent tube holes (121), arranged in eight rows and twelve columns.

3. A PCR reagent dispensing kit according to claim 2, characterized in that: There are a total of ten control tube holes (131), of which four are large holes and six are small holes, and the control tube holes (131) are arranged in five rows and two columns.

4. A PCR reagent dispensing kit according to claim 3, characterized in that: The length and width of the first marking cover (21) are greater than the length and width of the second marking cover (22) respectively, so as to distinguish them.

5. A PCR reagent dispenser according to claim 4, characterized in that: Both the first (21) and the second (22) of the marking cover are provided with marking cavities.

6. A PCR reagent dispenser according to any one of claims 1-5, characterized in that: The front and rear sides of the first boss (12) and the second boss (13) are provided with grooves (3), and the front and rear sides of the first mark cover (21) and the second mark cover (22) are provided with receiving cavities (23). The PCR reagent dispensing box also includes two sets of locking structures (4). The locking structure (4) includes a slider (41) located in the receiving cavity (23). On the opposite side of the two sets of sliders (41), there are limiting protrusions (42) that match the grooves (3). The locking structure (4) also includes an adjustment transmission component for moving the two sets of limiting protrusions (42) towards each other or away from each other.

7. A PCR reagent dispenser according to claim 6, characterized in that: Both the first marking cover (21) and the second marking cover (22) have a perforation at the center of their tops. The adjustment transmission component also includes a transmission shaft (43) rotatably connected to the inner side of the corresponding perforation. A meshing wheel (44) is fixed at the bottom of the transmission shaft (43). Transmission bars (45) are provided on the left and right sides of the meshing wheel (44). The transmission bars (45) are fixedly connected to the slide bar (41). Multiple meshing teeth are fixed on the side of the transmission bar (45) near the meshing wheel (44) so ​​that the transmission bar (45) meshes with the meshing wheel (44). The top of the transmission shaft (43) passes through the perforation and is fixed with a swivel cap (46). The diameter of the swivel cap (46) is larger than the diameter of the perforation.

8. A PCR reagent dispenser according to claim 7, characterized in that: The bottom of the nut (46) is provided with a mounting groove, and a spring (47) is provided in the mounting groove and sleeved on the outside of the drive shaft (43).

9. A PCR reagent dispenser according to claim 8, characterized in that: The inner top walls of the first marking cover (21) and the second marking cover (22) are provided with transmission grooves (24). The transmission grooves (24) are connected to the corresponding two sets of receiving cavities (23). The transmission bar (45) and the meshing wheel (44) in the locking structure (4) are located in the corresponding transmission grooves (24). A step is provided in the transmission groove (24). A panel (25) is fixed at the step by bolts. The transmission bar (45) is slidably connected to the upper surface of the panel (25).

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