A rapid detection device for fluorescent quantitative polymerase chain reaction

Abstract

The utility model provides a kind of superfast detection device of fluorescent quantitative polymerase chain reaction, it is related to molecular biology detection technical field, including: casing, the bottom position of the one side of the casing and the inside position of casing are equipped with firm plate respectively, the firm plate of bottom is equipped with first electric motor on one side, the bottom position of the firm plate of inside is equipped with third electric motor, the bottom position of casing is equipped with locating sleeve on the other side.Electric motor driven stirring sleeve simultaneously, synchronously stirs a group of reaction test tube, makes fluorescent quantitative polymerase chain fully mix in reaction test tube, the inside of stirring sleeve is provided with detection rod and the reaction of fluorescent quantitative polymerase chain is rapidly detected, the setting of an electric motor simplifies the drive structure and energy consumption of a group of stirring sleeve, detection rod, solves the problem that traditional integration structure is poor in adaptability, maintenance is complicated.

Description

Technical Field

[0001] This invention relates to the field of molecular biology detection technology, and in particular to an ultrafast detection device for quantitative fluorescent polymerase chain reaction. Background Technology

[0002] In the field of molecular biology detection, quantitative real-time polymerase chain reaction (qPCR) technology has advantages such as high specificity and high sensitivity. qPCR has been widely used in clinical diagnosis, food safety testing, and environmental monitoring. However, existing qPCR detection devices have the following technical shortcomings in practical applications:

[0003] Traditional real-time polymerase chain reaction (PCR) detection devices often use a single-size slot for positioning test tubes, which can only accommodate reaction test tubes of a specific diameter. When changing test tubes for different detection needs, the clamping components need to be disassembled and replaced. Furthermore, only one side can detect the reagents inside one test tube, making the operation process complicated and seriously affecting the detection efficiency.

[0004] Existing devices often use independent drives for reagent mixing and fluorescence detection, such as using multiple electric motors to control the stirring and detection components separately. This not only increases the overall size and energy consumption of the device, but also easily leads to inconsistent mixing speeds due to poor synchronization of multiple motors, resulting in differences in the uniformity of reagent mixing in multiple test tubes. At the same time, maintenance costs also increase.

[0005] Traditional devices require step-by-step steps for testing batches of samples, including tube clamping, reagent mixing, and fluorescence detection. Each step involves additional manual intervention; for example, the stirring component needs to mix each tube sequentially, and the detection component needs to collect signals individually. This results in a longer overall testing time, making it difficult to meet the needs of emergency testing and rapid clinical diagnosis, thus limiting the applicability of the device. Utility Model Content

[0006] This utility model relates to an ultrafast detection device for quantitative real-time polymerase chain reaction (qPCR). A first and third electric motor are mounted on a stabilizing plate within the housing, forming the power foundation of the device. A clamping mechanism with a threaded rod and a clamping frame with reverse threaded holes, driven by the first electric motor, synchronously clamps and positions the reaction tubes. The clamping groove is adaptable to tubes of different diameters and ensures stability. A lifting mechanism, relying on a dovetail sliding groove and gear meshing, drives the lifting frame to precisely raise and lower, achieving position switching between the stirring sleeve and the detection rod. A mixing mechanism, via a third electric motor, splined connection, and synchronous belt drive, drives multiple sets of stirring sleeves to rotate synchronously, completing uniform reagent mixing. The detection rod, aligned with the test tube by the lifting frame, collects fluorescence signals. The entire system forms an integrated ultrafast detection system for synchronous tube clamping, synchronous reagent mixing, precise raising and lowering, and batch detection, meeting the efficiency and accuracy requirements of qPCR.

[0007] This invention provides an ultrafast detection device for quantitative real-time polymerase chain reaction (qPCR), specifically comprising: a housing; stabilizing plates are respectively provided on one side of the housing at the bottom position and on the inner side of the housing; a first electric motor is installed on one side of the bottom stabilizing plate, and a third electric motor is installed at the bottom position of the inner stabilizing plate; a positioning sleeve is provided on the other side of the bottom position of the housing; symmetrically distributed clamping frames are installed on the inner side of the housing; a set of reaction tubes are installed on the inner side of the clamping frames; a stabilizing frame is installed on the inner side of the housing; a vertical lifting frame is installed on one side of the stabilizing frame; a set of evenly distributed support frames are installed above the lifting frame; the support frames have a U-shaped structure; stabilizing holes are respectively opened on one side of the support frames, and detection rods are inserted into the stabilizing holes.

[0008] Furthermore, a rotatably connected threaded rod is installed at the bottom of the housing. One side of the threaded rod extends into the interior of the positioning sleeve and is rotatably connected to the positioning sleeve. The other side of the threaded rod is connected to the drive shaft of the first electric motor. The threaded rod has a double thread structure. The first electric motor, the threaded rod, and the clamping frame cooperate with each other to form a clamping mechanism.

[0009] Furthermore, the bottom of the housing is provided with evenly distributed sliding holes, and the bottom of the clamping frame is provided with a set of vertical sliding blocks. The sliding blocks are rectangular in structure and pass through the interior of the sliding holes. The inner side of the clamping frame is provided with clamping grooves. The clamping grooves are V-shaped and there are three clamping grooves. The bottom of the clamping frame is provided with threaded holes in the middle position. The threads of the threaded holes are opposite in direction. The threaded rod passes through the interior of the threaded hole and is threadedly connected to the threaded hole. The thread pitch is processed according to actual needs to ensure that the threaded rod and the threaded hole are effectively threadedly connected. The edge of the reaction tube extends into the interior of the clamping groove.

[0010] Furthermore, a second electric motor is installed at the top of the stabilizing frame, and a drive gear is installed on the outer side of the drive shaft of the second electric motor. Bolt mounting holes are located at the top and bottom of the stabilizing frame and at the corners of the second electric motor.

[0011] Furthermore, a vertical sliding groove is provided on one side of the upper position of the stabilizing frame. The sliding groove has a dovetail structure. A positioning groove is provided on one side of the lifting frame. The positioning groove has a rectangular structure. A sliding strip is installed on the inner side of the positioning groove. The positioning groove and the sliding strip are each set to one. The sliding strip extends into the interior of the sliding groove. A set of meshing teeth is provided on one side of the lifting frame. The meshing teeth mesh with the gear installed on the second electric motor.

[0012] Furthermore, a set of evenly distributed positioning grooves are provided at the upper position of the support frame. The positioning grooves are U-shaped structures, and the bottom of the support frame extends into the interior of the positioning grooves. A set of vertical bolt mounting holes are provided on the base of the support frame.

[0013] Furthermore, a positioning seat is installed at a corner on one side of the lifting frame, and a vertical rotating shaft is installed on the inner side of the positioning seat. A set of bolt mounting holes are opened on both sides of the positioning seat. The stabilizing frame, the second electric motor, the lifting frame, and the positioning seat cooperate with each other to form a lifting mechanism. A docking groove is opened at the bottom of the rotating shaft. The docking groove has a spline structure. A set of docking blocks corresponding to the docking groove is provided at the upper position of the drive shaft of the third electric motor. The docking blocks and the docking groove engage to control the third electric motor to drive the rotating shaft to rotate. A drive pulley is installed at the bottom of the rotating shaft.

[0014] Furthermore, a vertical mounting hole is provided on one side of the support frame, and bearings are installed inside the mounting hole. Vertical stirring sleeves are inserted inside the bearings. The connection between the bearings, mounting holes and stirring sleeves is stabilized by friction surfaces. Pulleys are installed on the outer side of the stirring sleeves. A synchronous belt is installed between a set of pulleys and the drive pulley of the rotating shaft. The third electric motor, rotating shaft, support frame, stirring sleeves and synchronous belt cooperate to form a mixing mechanism. A set of stirring bars is provided on the outer side of the stirring sleeve.

[0015] This invention provides an ultrafast detection device for quantitative real-time polymerase chain reaction (qPCR), which has the following advantages:

[0016] The clamping mechanism in this invention uses a double-threaded rod in conjunction with a clamping frame with a reverse-threaded hole. Driven by a first electric motor, the two clamping frames move synchronously in opposite directions, which can quickly complete the clamping and loosening of reaction tubes and greatly simplify the operation process. A set of V-shaped clamping grooves is provided to accommodate reaction tubes of different diameters. At the same time, a group of reaction tubes can be clamped and positioned simultaneously, which solves the problems of poor stability and insecure fixation of reaction tubes.

[0017] An electric motor-driven stirring sleeve is installed to simultaneously and synchronously stir a group of reaction tubes, ensuring thorough mixing of the fluorescent quantitative polymerase chain in the reaction tubes. The inside of the stirring sleeve is equipped with a detection rod for rapid detection of the fluorescent quantitative polymerase chain reaction. The electric motor simplifies the driving structure of a group of stirring sleeves and detection rods, as well as energy consumption, and solves the problems of poor adaptability and cumbersome maintenance of traditional integrated structures.

[0018] Specifically, a lifting mechanism is set up to control the up and down movement of the stirring sleeve and the detection rod. When the stirring sleeve and the detection rod move upward, it facilitates the placement and removal of the reaction tube. When the stirring sleeve and the detection rod move downward, they can extend into the interior of the reaction tube. The lifting mechanism allows the stirring sleeve and the detection rod to be precisely aligned with the test tube, avoiding the impact of displacement deviation on the mixing uniformity or detection accuracy, and improving the stability of the device operation.

[0019] The stirring sleeve, detection rod, lifting mechanism, and mixing mechanism ensure consistent mixing speed of reagents in multiple test tubes and enable simultaneous detection of reagents in multiple test tubes. Combined with the stirring strip, it enhances the liquid agitation effect, accelerates reagent mixing speed, shortens pretreatment time, and meets the core requirements of ultra-fast detection. Attached Figure Description

[0020] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings of the embodiments will be briefly described below.

[0021] The accompanying drawings described below are only related to some embodiments of the present invention and are not intended to limit the scope of the present invention.

[0022] In the attached diagram:

[0023] Figure 1 A schematic diagram of the axial structure of the ultrafast detection device of this invention in the detection state after assembly is shown.

[0024] Figure 2 A schematic diagram of the axial structure of the hybrid mechanism of this utility model after it rises is shown;

[0025] Figure 3 This utility model illustrates Figure 2 A schematic diagram of the axonal structure from an elevation viewpoint;

[0026] Figure 4 A schematic diagram of the axial structure of the shell and the mixing mechanism of this utility model is shown in cross-section.

[0027] Figure 5 A partial axial side view of the lifting mechanism and clamping mechanism of this utility model is shown;

[0028] Figure 6 The diagram shows a cross-sectional view of the support frame and stirring sleeve of this utility model.

[0029] List of reference numerals

[0030] 1. Shell;

[0031] 2. Clamping mechanism; 201. First electric motor; 202. Threaded rod; 203. Clamping frame;

[0032] 3. Reaction tubes;

[0033] 4. Lifting mechanism; 401. Stabilizing frame; 402. Second electric motor; 403. Lifting frame; 404. Positioning seat;

[0034] 5. Mixing mechanism; 501. Third electric motor; 502. Rotating shaft; 503. Support frame; 504. Mixing sleeve; 505. Synchronous belt;

[0035] 6. Detection rod. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the described embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0037] Example 1: Please refer to Figures 1 to 6 :

[0038] This invention proposes an ultrafast detection device for quantitative real-time polymerase chain reaction (qPCR), comprising: a housing 1; stabilizing plates are respectively provided on one side of the housing 1 at the bottom position and on the inner side of the housing 1; a first electric motor 201 is mounted on one side of the bottom stabilizing plate; a threaded rod 202 is rotatably connected to the bottom position of the housing 1; one side of the threaded rod 202 extends into the interior of a positioning sleeve and is rotatably connected to the positioning sleeve; the other side of the threaded rod 202 is connected to the drive shaft of the first electric motor 201; the threaded rod 202 has a double-threaded structure; and the first electric motor 201 and the threaded rod 202... 02. The clamping frames 203 cooperate with each other to form the clamping mechanism 2. The connection position of the threaded rod 202 and the drive shaft is set with keyway circumferential positioning according to the existing technology. Specifically, the keyway circumferential positioning ensures that the drive shaft of the first electric motor 201 and the threaded rod 202 rotate synchronously. The threaded rod 202 with double thread structure cooperates with the threaded hole of the clamping frame 203 to realize the synchronous movement of the two clamping frames 203 towards or in opposite directions, quickly completing the clamping or releasing steps of the reaction test tube 3. At the same time, the positioning sleeve provides stable support for the threaded rod 202 to prevent radial shaking when it rotates, ensuring the operational stability of the clamping mechanism 2.

[0039] In this embodiment, a third electric motor 501 is installed at the bottom of the inner stabilizing plate. A positioning sleeve is provided on the other side of the bottom of the housing 1. One positioning sleeve is provided. Two symmetrically distributed clamping frames 203 are installed on the inner side of the housing 1. A set of reaction tubes 3 are installed on the inner side of the clamping frames 203. Three evenly distributed sliding holes are provided at the bottom of the housing 1. The number of sliding holes can be increased or decreased according to actual needs. The sliding holes are rectangular. A set of vertical sliding blocks, also rectangular, are provided at the bottom of the clamping frames 203. The sliding blocks pass through the interior of the sliding holes and, in conjunction with the sliding holes, position the clamping frames 203 circumferentially, ensuring that the clamping frames 203 move smoothly only along the direction of the sliding holes. A set of evenly distributed sliding holes is provided on the inner side of the clamping frames 203. The clamping grooves are V-shaped and three in number. The number of clamping grooves can be increased or decreased according to actual needs. The bottom of the clamping frame 203 has threaded holes at the center position, with opposite thread directions. A threaded rod 202 passes through the threaded holes and is threadedly connected to them. The thread pitch is machined according to actual needs to ensure effective threaded connection between the threaded rod 202 and the threaded hole. The edge of the reaction tube 3 extends into the clamping grooves. Specifically, the reverse threaded holes adapt to the double-threaded rod 202, enabling the two clamping frames 203 to move synchronously and symmetrically, ensuring the reaction tube 3 remains centered. The V-shaped clamping grooves can accommodate reaction tubes 3 of different diameters. The inclined surfaces on both sides tightly fit the outer wall of the tube, enhancing clamping stability and preventing the tube from loosening or falling off during subsequent mixing or lifting processes.

[0040] In this embodiment, a stabilizing frame 401 is installed on the inner side of the housing 1. One stabilizing frame 401 is set up. A vertical lifting frame 403 is installed on one side of the stabilizing frame 401. One lifting frame 403 is set up. A set of evenly distributed support frames 503 is installed above the lifting frame 403. The support frames 503 have a U-shaped structure. Three support frames 503 are set up. The number of support frames 503 can be increased or decreased according to actual needs. A stabilizing hole is opened on one side of each support frame 503. A detection rod 6 is inserted into the inside of each stabilizing hole. One detection rod 6 is installed inside each stabilizing hole.

[0041] In this embodiment, a second electric motor 402 is installed above the stabilizing frame 401. A drive gear is installed on the outer side of the drive shaft of the second electric motor 402. There is one drive gear. Bolt mounting holes are located at the top and bottom of the stabilizing frame 401 and at the corners of the second electric motor 402. Matching bolts are installed in the bottom bolt mounting holes to stabilize the stabilizing frame 401. Matching bolts are installed in the corner bolt mounting holes to secure the second electric motor 402. A vertical sliding groove with a dovetail structure is provided on one side of the top of the stabilizing frame 401. A positioning groove with a rectangular structure is provided on one side of the lifting frame 403. A sliding strip is installed on the inner side of the positioning groove. Each of the positioning groove and the sliding bar is configured as one, with the sliding bar extending into the interior of the sliding groove. The stabilizing frame 401, in conjunction with the sliding groove and the sliding bar, provides lateral and circumferential positioning for the lifting frame 403. A set of meshing teeth is provided on one side of the lifting frame 403, which mesh with the gear mounted on the second electric motor 402. Specifically, the dovetail-structured sliding groove and the sliding bar cooperate to form a bidirectional limiting mechanism, which restricts the lateral displacement of the lifting frame 403 and prevents it from rotating circumferentially, ensuring that the lifting frame 403 moves smoothly only vertically. The meshing transmission between the meshing teeth and the drive gear converts the rotational motion of the second electric motor 402 into the linear motion of the lifting frame 403, thereby achieving precise lifting control of the support frame 503, the detection rod 6, and the mixing mechanism 5, and meeting the positional requirements of the reaction tube 3 at different stages.

[0042] In this embodiment, a set of evenly distributed positioning grooves are formed at the upper position of the support frame 503. The positioning grooves have a U-shaped structure, and the bottom of the support frame 503 extends into the interior of the positioning grooves. A set of vertical bolt mounting holes are formed on the base of the support frame 503. Matching bolts are installed in the bolt mounting holes according to the actual situation. After the bolts are installed, the support frame 503 is stably installed above the lifting frame 403. Specifically, the U-shaped positioning grooves pre-position the support frame 503, ensuring the accurate installation position of the support frame 503; simultaneously, according to testing requirements, through... The bolts allow for quick replacement of different quantities or specifications of support frames 503, enhancing the adaptability and ease of maintenance of the lifting device. A positioning seat 404 is installed at a corner on one side of the lifting frame 403. A vertical rotating shaft 502 is installed inside the positioning seat 404. A rotating ring is installed between the rotating shaft 502 and the positioning seat 404, following existing conventional structures, to position the lifting frame 403 vertically relative to the rotating shaft 502. A set of bolt mounting holes is provided on both sides of the positioning seat 404, allowing for the installation of matching bolts as needed. The bolts are installed to securely connect the positioning seat 404 and the lifting frame 403. The stabilizing frame 401, the second electric motor 402, the lifting frame 403, and the positioning seat 404 work together to form the lifting mechanism 4. The bottom of the rotating shaft 502 has a splined groove. The drive shaft of the third electric motor 501 has a set of connecting blocks corresponding to the connecting groove at the top. The connecting blocks and the connecting groove engage, and with the cooperation of the connecting blocks and the connecting groove, the drive shaft and the rotating shaft 502 can be flexibly connected at any angle, controlling the third electric motor. Motor 501 drives rotating shaft 502 to rotate. A drive pulley is installed at the bottom of rotating shaft 502. Specifically, the rotating ring allows rotating shaft 502 to rotate freely within positioning seat 404, while simultaneously achieving vertical positioning. After positioning seat 404 is securely connected to lifting frame 403, it can rise and fall synchronously with lifting frame 403. The spline structure mating groove and mating block engage to achieve precise power transmission between the third electric motor 501 drive shaft and rotating shaft 502. The drive pulley provides a mounting base for synchronous belt 505, ensuring that the mixing mechanism 5 obtains stable power.

[0043] In this embodiment, a vertical mounting hole is provided on one side of the support frame 503. Bearings are installed inside the mounting hole, and vertical stirring sleeves 504 are inserted inside the bearings. The connection between the bearings, mounting holes, and stirring sleeves 504 is stabilized by friction surfaces, so that the support frame 503, in conjunction with the bearings, positions the stirring sleeves 504 vertically. The number of stirring sleeves 504 is selected according to the number of mounting holes. In this invention, one mounting hole is provided. After the lifting frame 403 moves downward, the stirring sleeve 504 extends into the interior of the reaction tube 3. Pulleys are installed on the outer side of the stirring sleeve 504. A synchronous belt 505 is installed between a set of pulleys and the drive pulley of the rotating shaft 502, engaging the pulleys and the synchronous belt 505. The third electric motor 501, rotating shaft 502, support frame 503, stirring sleeve 504, and synchronous belt 505 cooperate to form a mixing mechanism 5, which mixes the reaction tubes and the reaction tubes. A single synchronous belt 505 is installed, and a set of pulleys are connected in series after the synchronous belt 505 is installed. When the synchronous belt 505 rotates, it drives a set of stirring sleeves 504 to rotate simultaneously. A set of stirring bars are provided on the outer side of the stirring sleeves 504. The shape of the stirring teeth and stirring bars on the outer side of the stirring sleeves 504 is selected according to actual needs. Specifically, the bearings reduce the frictional resistance when the stirring sleeves 504 rotate, and at the same time, they cooperate with the mounting holes to position the stirring sleeves 504 vertically. The synchronous belt 505 drives the pulleys in series to realize the synchronous transmission of power from the third electric motor 501, so that the speed of the set of stirring sleeves 504 is consistent, ensuring that the liquid in the multiple reaction tubes 3 is mixed with the same uniformity. The stirring bars enhance the liquid disturbance effect, accelerate the mixing speed of the reaction reagents, shorten the pretreatment time, and the stirring sleeves 504 can flexibly enter and exit the reaction tubes 3 with the lifting frame 403, avoiding interference with the detection process and improving the sensitivity of the device.

[0044] Example 2, based on Example 1, such as Figures 1-5 As shown, a controller needs to be installed on the housing 1, and the controller is electrically connected to the first electric motor 201, the second electric motor 402, and the third electric motor 501 respectively, referring to the prior art.

[0045] Example 3, based on Example 1, such as Figure 1 As shown, a dust cover is installed on the top of the housing 1 to protect the clamping mechanism 2, lifting mechanism 4, and mixing mechanism 5 from dust, according to actual needs.

[0046] The working principle of this embodiment:

[0047] First, install the clamping mechanism 2, lifting mechanism 4, and mixing mechanism 5 inside the housing 1 in the above order and steps. Then, connect the controller to the first electric motor 201, the second electric motor 402, and the third electric motor 501 in accordance with existing technology.

[0048] Start the first electric motor 201, which drives the threaded rod 202 to rotate. Because the threaded holes at the bottom of the clamping frame 203 are in opposite directions, the two clamping frames 203 move synchronously in opposite directions along the rectangular sliding hole at the bottom of the shell 1, forming an open state. Place the reaction tubes 3 containing PCR reaction reagents into the clamping grooves inside the clamping frame 203 one by one. Start the first electric motor 201 again, so that the threaded rod 202 rotates in the opposite direction and drives the two clamping frames 203 to move towards each other until the clamping grooves clamp the reaction tubes 3. The clamping force needs to be controlled to avoid clamping the reaction tubes 3 too tightly and causing damage. Turn off the first electric motor 201 to complete the clamping of the reaction tubes 3.

[0049] The second electric motor 402 is started using a traditional controller. The second electric motor 402 drives the gear to rotate. The gear meshes with the meshing teeth on one side of the lifting frame 403. The gear drives the lifting frame 403 to move downward along the dovetail sliding groove of the stabilizing frame 401 until the stirring bar on the stirring sleeve 504 is fully inserted into the reagent in the reaction tube 3. The second electric motor 402 is then paused. The third electric motor 501 is started. Its drive shaft meshes with the rotating shaft 502 through a spline joint groove. This drives the drive pulley at the bottom of the rotating shaft 502 to rotate. The synchronous belt 505 drives the pulley on the outside of the stirring sleeve 504 to rotate, so that the stirring sleeve 504 starts to rotate. The stirring time can be adjusted by the controller according to the reagent mixing requirements to achieve uniform mixing of the reagent.

[0050] After the reagents are mixed, the third electric motor 501 is turned off, and the stirring sleeve 504 stops rotating. The detection rod 6 is started, or inserted into the liquid surface to a certain depth as needed, the specific depth of which is set according to the detection plan. When the detection rod 6 reaches the preset detection position, the second electric motor 402 is paused, and the detection system is started. The detection rod 6 collects the fluorescence signal of the reagent in the reaction tube 3 and transmits the signal to the controller. The controller analyzes the fluorescence signal in real time, generates an amplification curve, and records the detection data. According to the preset detection cycle, multiple rounds of fluorescence signal collection are completed until the PCR reaction is finished.

[0051] After the real-time polymerase chain reaction is completed, start the second electric motor 402 to raise the lifting frame 403 to the initial high position and turn off the power of the detection device; start the first electric motor 201 to make the clamping frame 203 move in the opposite direction and open, take out the reaction tube 3, and properly dispose of the waste liquid and the tube; clean and disinfect the components of the clamping groove and stirring sleeve 504, organize the detection data and export the report to complete the detection process.

Claims

1. An ultrafast detection device for quantitative real-time polymerase chain reaction, characterized in that, include: The shell (1), the lifting frame (403) and the detection rod (6) are provided. The shell (1) has a stabilizing plate at the bottom and the inner side of the shell (1). The bottom stabilizing plate is equipped with a first electric motor (201) on one side and a third electric motor (501) at the bottom of the inner stabilizing plate. The bottom of the shell (1) is equipped with a positioning sleeve on the other side. The inner side of the shell (1) is equipped with symmetrically distributed clamping frames (203). A set of reaction tubes (3) is installed on the inner side of the clamping frames (203). The inner side of the shell (1) is equipped with a stabilizing frame (401). A vertical lifting frame (403) is installed on one side of the stabilizing frame (401). A set of evenly distributed support frames (503) is installed above the lifting frame (403). A stabilizing hole is opened on one side of the support frame (503). The detection rod (6) is inserted into the inside of the stabilizing hole.

2. The ultrafast detection device for quantitative real-time polymerase chain reaction according to claim 1, characterized in that, A rotatably connected threaded rod (202) is installed at the bottom of the housing (1). One side of the threaded rod (202) extends into the interior of the positioning sleeve. One side of the threaded rod (202) is rotatably connected to the positioning sleeve. The other side of the threaded rod (202) is connected to the drive shaft of the first electric motor (201). The first electric motor (201), the threaded rod (202), and the clamping frame (203) cooperate with each other to form a clamping mechanism (2).

3. The ultrafast detection device for quantitative real-time polymerase chain reaction according to claim 1, characterized in that, The bottom of the housing (1) is provided with evenly distributed sliding holes. The bottom of the clamping frame (203) is provided with a set of vertical sliding blocks. The sliding blocks pass through the interior of the sliding holes. The inner side of the clamping frame (203) is provided with a clamping groove. The bottom of the clamping frame (203) is provided with threaded holes in the middle position. The threads of the threaded holes are opposite in direction. The threaded rod (202) passes through the interior of the threaded hole and is threadedly connected to the threaded hole. The thread pitch is processed according to actual needs so that the threaded rod (202) and the threaded hole are effectively threadedly connected. The edge of the reaction tube (3) extends into the interior of the clamping groove.

4. The ultrafast detection device for quantitative real-time polymerase chain reaction according to claim 1, characterized in that, A second electric motor (402) is installed at the top of the stabilizer (401). A drive gear is installed on the outer side of the drive shaft of the second electric motor (402). Bolt mounting holes are located at the top and bottom of the stabilizer (401) and at the corners of the second electric motor (402).

5. The ultrafast detection device for quantitative real-time polymerase chain reaction according to claim 1, characterized in that, The upper part of the stabilizing frame (401) has a vertical sliding groove on one side, and the lifting frame (403) has a positioning groove on one side. A sliding strip is installed on the inner side of the positioning groove and extends into the interior of the sliding groove. A set of meshing teeth is provided on one side of the lifting frame (403), and the meshing teeth mesh with the gear installed on the second electric motor (402).

6. The ultrafast detection device for quantitative real-time polymerase chain reaction according to claim 1, characterized in that, A set of evenly distributed positioning grooves are provided at the upper position of the support frame (503), the bottom of the support frame (503) extends into the interior of the positioning grooves, and a set of vertical bolt mounting holes are provided on the base of the support frame (503).

7. The ultrafast detection device for quantitative real-time polymerase chain reaction according to claim 1, characterized in that, A positioning seat (404) is installed at a corner on one side of the lifting frame (403). A vertical rotating shaft (502) is installed on the inner side of the positioning seat (404). A set of bolt mounting holes are opened on both sides of the positioning seat (404). The stabilizing frame (401), the second electric motor (402), the lifting frame (403), and the positioning seat (404) cooperate to form a lifting mechanism (4). A docking groove is opened at the bottom of the rotating shaft (502). A set of docking blocks corresponding to the docking groove is provided on the upper position of the drive shaft of the third electric motor (501). The docking blocks and the docking groove are engaged, controlling the third electric motor (501) to drive the rotating shaft (502) to rotate. A drive pulley is installed at the bottom of the rotating shaft (502).

8. The ultrafast detection device for quantitative real-time polymerase chain reaction according to claim 1, characterized in that, The support frame (503) has a vertical mounting hole on one side, and bearings are installed inside the mounting hole. Vertical stirring sleeves (504) are inserted inside the bearings. The connection between the bearings, the mounting hole and the stirring sleeves (504) is stabilized by friction surfaces. Pulleys are installed on the outer side of the stirring sleeves (504). A synchronous belt (505) is installed between a set of pulleys and the drive pulley of the rotating shaft (502). The third electric motor (501), the rotating shaft (502), the support frame (503), the stirring sleeves (504) and the synchronous belt (505) cooperate to form a mixing mechanism (5). A set of stirring bars is provided on the outer side of the stirring sleeves (504).

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