A 96-well PCR plate

Abstract

The utility model relates to PCR plate technical field especially relates to a 96 hole PCR plate, including the plate body, the plate body evenly is provided with several reaction tubes, the hole wall thickness of reaction tube is linearly reduced along the radial distance from the center to the periphery, the hole wall of reaction tube includes plastic base body, heat conducting metal foil, insulating layer, the hole wall thickness is linearly reduced along the radial distance from the center to the periphery, can effectively reduce the thermal resistance, the reaction tube of periphery can be in contact with the heat source completely, reduces the thermal loss and appears the edge effect, and the heat conducting metal foil is inbedded in the reaction tube, and the plastic base body is combined and conducts heat, and the heat conducting metal foil does not contact with the reaction liquid directly, avoids the influence PCR reaction, can effectively improve the heat conducting performance of plastic base body simultaneously, and the heating rate is fast, effectively solves the heat loss difference, reduces the biological pollution risk.

Description

Technical fields:

[0001] This utility model relates to the field of PCR plate technology, and in particular to a 96-well PCR plate. Background technology:

[0002] PCR plates are used in polymerase chain reaction (PCR) to carry primers, Taq DNA polymerase, dNTPs, template nucleic acids, Mg, buffer, and other components involved in the amplification reaction. However, when used in thermal cyclers, wells located at the edges and corners of the plate may exhibit different temperature changes and final temperatures compared to the central wells due to varying contact areas with the heat source or faster heat dissipation via radiation / convection. This can lead to inconsistent amplification efficiencies on the same plate, affecting the accuracy of results and making the plate inconvenient to use. Therefore, there is an urgent need for a 96-well PCR plate with superior thermal conductivity. Utility Model Content:

[0003] The purpose of this invention is to provide a 96-well PCR plate that has strong thermal conductivity and a fast heating rate, addressing the shortcomings of existing technologies.

[0004] To achieve the above objectives, the technical solution adopted by this utility model is: a 96-well PCR plate, comprising a plate body, wherein a plurality of reaction tubes are uniformly arranged on the plate body, the wall thickness of the reaction tubes decreases linearly from the center to the periphery along the radial distance, and the wall of the reaction tubes comprises a plastic substrate, a thermally conductive metal foil, and an insulating layer, wherein the thermally conductive metal foil is embedded in the plastic substrate, and the insulating layer is coated on the surface of the thermally conductive metal foil.

[0005] A further improvement to the above scheme is that the thermally conductive metal foil is either aluminum foil or copper foil.

[0006] A further improvement to the above scheme is that the reaction tube is set in a conical shape.

[0007] A further improvement to the above scheme is that the top edge of the reaction tube extends beyond the top of the plate.

[0008] A further improvement to the above solution is that the plate is integrally molded from PEEK engineering plastic.

[0009] A further improvement to the above solution is that a foolproof chamfer is provided at one corner of the plate.

[0010] A further improvement to the above scheme is that a number of positioning holes are evenly arranged on the plate.

[0011] A further improvement to the above scheme is that the bottom of the reaction tube is provided with a number of heat dissipation fins, and the number of heat dissipation fins are evenly arranged in an inverted triangle on the bottom hole wall of the reaction tube.

[0012] The beneficial effects of this utility model are as follows: This utility model includes a plate body, on which a plurality of reaction tubes are uniformly arranged. The wall thickness of the reaction tube decreases linearly from the center to the periphery along the radial distance. The wall of the reaction tube includes a plastic matrix, a thermally conductive metal foil, and an insulating layer. The thermally conductive metal foil is embedded in the plastic matrix, and the insulating layer is coated on the surface of the thermally conductive metal foil.

[0013] The wall thickness of the pores in this invention decreases linearly from the center outwards along the radial distance, which effectively reduces thermal resistance. The reaction tubes at the periphery can fully contact the heat source, reducing heat loss and edge effects. The reaction tubes are embedded with thermally conductive metal foils, which combine with the plastic matrix for heat conduction. The thermally conductive metal foils do not directly contact the reaction liquid, avoiding interference with the PCR reaction. At the same time, they can effectively improve the thermal conductivity of the plastic matrix, resulting in a fast heating rate, effectively solving the problem of heat loss differences, and reducing the risk of biological contamination. Attached image description:

[0014] Figure 1 This is a schematic diagram of the structure of this utility model.

[0015] Figure 2 This is a cross-sectional view of the reaction tube of this utility model.

[0016] Explanation of reference numerals in the attached drawings: 1. Plate body; 12. Foolproof chamfer; 13. Positioning hole; 2. Reaction tube; 21. Plastic substrate; 22. Thermally conductive metal foil; 23. Insulating layer; 24. Heat dissipation fins. Detailed implementation method:

[0017] The present invention will be further described below with reference to the accompanying drawings, such as... Figure 1-2 As shown, this utility model provides a 96-well PCR plate, including a plate body 1. The plate body 1 is characterized by the uniform arrangement of several reaction tubes 2. The wall thickness of the reaction tubes 2 decreases linearly from the center outwards along the radial distance. The wall of each reaction tube 2 includes a plastic substrate 21, a thermally conductive metal foil 22, and an insulating layer 23. The thermally conductive metal foil 22 is embedded within the plastic substrate 21, and the insulating layer 23 is coated on the surface of the thermally conductive metal foil 22. The linear decrease in wall thickness from the center outwards along the radial distance effectively reduces thermal resistance. The reaction tubes 2 at the periphery can fully contact the heat source, reducing heat loss and edge effects. The thermally conductive metal foil 22 is embedded within the reaction tubes 2 and combined with the plastic substrate 21 for heat conduction. The thermally conductive metal foil 22 does not directly contact the reaction solution, avoiding interference with the PCR reaction. Simultaneously, it effectively improves the thermal conductivity of the plastic substrate 21, resulting in a faster heating rate, effectively addressing differences in heat dissipation, and reducing the risk of biological contamination.

[0018] In some embodiments of this utility model, the plastic substrate 21 is injection molded from PP material, and the thermally conductive metal foil 22 is embedded in the plastic substrate 21 by laser micro-welding, resulting in a stable connection.

[0019] The present invention uses 22 pieces of thermally conductive metal foil, which are either aluminum foil or copper foil. Compared with the inefficient thermal conductivity of plastic layers, aluminum foil or copper foil can effectively improve thermal conductivity.

[0020] The reaction tube 2 of this invention is set in a conical shape. The bottom of the conical reaction tube 2 can break the thermal boundary layer, promote the formation of liquid vortex, and accelerate heat exchange.

[0021] The top edge of the reaction tube 2 of this invention extends beyond the top of the plate body 1, reducing liquid splashing when the lid is opened and reducing cross-contamination.

[0022] The plate 1 of this utility model is integrally molded from PEEK engineering plastic, which can effectively reduce thermal creep deformation and improve structural strength compared with traditional polypropylene.

[0023] One corner of the plate body 1 of this utility model is provided with a foolproof chamfer 12, which facilitates the machine to identify the direction of the perforated plate.

[0024] The plate body 1 of this utility model is evenly provided with a number of positioning holes 13 to ensure accurate machine recognition and reduce errors.

[0025] The bottom of the reaction tube 2 of this utility model is provided with a number of heat dissipation fins 24. The number of heat dissipation fins 24 are evenly arranged in an inverted triangle on the bottom hole wall of the reaction tube 2, which can effectively increase the heat transfer area and improve the heat transfer efficiency. At the same time, the tip of the heat dissipation fins 24 can generate periodic eddies, which can destroy the thermal boundary layer inside the reaction tube 2 and improve the convective heat transfer coefficient.

[0026] In some embodiments of this utility model, the surface of the heat dissipation fins 24 can be hydrophilic, which can guide the liquid to rise, form a thin liquid film to accelerate evaporation and cooling, and facilitate use.

[0027] Of course, the above description is only a preferred embodiment of the present utility model. Therefore, all equivalent changes or modifications made to the structure, features and principles described in the claims of the present utility model patent application are included in the scope of the present utility model patent application.

Claims

1. A 96-well PCR plate comprising a plate body (1), characterized in that: A plurality of reaction tubes (2) are uniformly arranged on the plate (1). The wall thickness of the reaction tube (2) decreases linearly from the center to the periphery along the radial distance. The wall of the reaction tube (2) includes a plastic substrate (21), a thermally conductive metal foil (22) sheet, and an insulating layer (23). The thermally conductive metal foil (22) sheet is embedded in the plastic substrate (21), and the insulating layer (23) is coated on the surface of the thermally conductive metal foil (22) sheet.

2. A 96-well PCR plate according to claim 1, characterized in that: The thermally conductive metal foil (22) is either aluminum foil or copper foil.

3. The 96-well PCR plate of claim 1, wherein: The reaction tube (2) is configured as a cone shape.

4. The 96-well PCR plate of claim 1, wherein: The top edge of the reaction tube (2) extends out of the top of the plate (1).

5. The 96-well PCR plate of claim 1, wherein: The plate (1) is integrally molded from PEEK engineering plastic.

6. The 96-well PCR plate of claim 1, wherein: One corner of the plate (1) is provided with a foolproof chamfer (12).

7. The 96-well PCR plate of claim 1, wherein: The plate (1) is provided with a number of positioning holes (13) evenly distributed.

8. The 96-well PCR plate of claim 1, wherein: The bottom of the reaction tube (2) is provided with a number of heat dissipation fins (24), which are arranged in an inverted triangle on the bottom hole wall of the reaction tube (2).

Images