An ultramicro spectrophotometer cuvette detection structure

Abstract

The utility model discloses a kind of ultramicro spectrophotometer cuvette detection structure, including operation platform, the rotating seat on the top of operation platform is rotatably connected with flip cover, main road optical fiber is equipped on the operation platform, the bottom of operation platform is also installed with cuvette placing box.The utility model passes through adopting double optical path design, pedestal and cuvette light path are independent, integrated design, without physical replacement spare part, by software selection "pedestal" or "cuvette card slot" mode, can effectively avoid cross contamination, when cuvette detection, directly cuvette is placed in the fixed slot beside pedestal, light path passes through incident optical fiber, aligns cuvette light transmission hole, the utility model structure is simple, without complex light path switching system, effectively solve the positioning problem and light energy loss problem of physical switching, improve the range of measurement, accuracy and reliability of equipment, convenient operation, convenient to carry, with higher practical value.

Description

Technical Field

[0001] This utility model relates to the field of spectrophotometer technology, specifically to a cuvette detection structure for an ultra-micro spectrophotometer. Background Technology

[0002] An ultra-micro spectrophotometer is an optical analytical instrument used to measure extremely small amounts of samples (typically 0.5-2 μL). By detecting the absorption or reflection characteristics of a sample to light of a specific wavelength, it can quickly analyze the concentration and purity of biomolecules such as nucleic acids and proteins. It uses the law of light absorption (also known as Lambert-Beer's law) to measure the absorbance (OD value) of a sample to ultraviolet / visible light and calculate the concentration of the target substance.

[0003] Currently, when switching between cuvette mode (conventional sample cell) and pedestal mode (micro-volume direct detection) in ultra-micro spectrophotometers, the optical system needs to simultaneously adjust the optical path, optical path length, and detection parameters to adapt to the detection requirements of different sample shapes. This typically involves mechanical adjustments to the mirror and aperture, resetting the optical path system (from the fixed optical path of the cuvette to the variable optical path of the pedestal), and switching the detector and signal processing. This increases the number of components, complicates the instrument structure, and makes the switching process cumbersome. To address these issues, we propose a cuvette detection structure for ultra-micro spectrophotometers to solve the problems mentioned in the background. Utility Model Content

[0004] The purpose of this invention is to provide a cuvette detection structure for an ultra-micro spectrophotometer to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cuvette detection structure for an ultra-micro spectrophotometer, comprising an operating table, a flip cover rotatably connected to a rotating seat on the top of the operating table, a main optical fiber provided on the operating table, a cuvette placement box installed at the bottom of the operating table, a cuvette body inside the cuvette placement box, and a lower receiver and an auxiliary optical fiber respectively installed on the left and right sides of the cuvette placement box.

[0006] Furthermore, a receiving groove is provided at the bottom of the inner wall of the cuvette placement box, and the bottom part of the cuvette body is disposed inside the receiving groove.

[0007] Furthermore, waist-shaped springs are symmetrically installed on the inner wall of the cuvette placement box, and anti-slip pads are adhered to the outer surface of the waist-shaped springs.

[0008] Furthermore, a light-shielding ring is installed on the flip cover.

[0009] Furthermore, a light-transmitting hole is provided on the outside of the cuvette placement box, and the position of the light-transmitting hole corresponds to that of the auxiliary optical fiber.

[0010] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0011] This invention employs a dual-optical-path design, with independent optical paths for the base and cuvette, integrated into a single unit. This eliminates the need for physical replacement of parts. Software selection of either the "base" or "cuvette slot" mode effectively prevents cross-contamination. During cuvette testing, the cuvette is simply placed in the fixed slot next to the base, and the optical path, via the incident fiber, is aligned with the cuvette's light-transmitting aperture. This invention features a simple structure, eliminating the need for a complex optical path switching system. It effectively solves the positioning and light energy loss problems associated with physical switching, improving the measurement range, accuracy, and equipment reliability. It is easy to operate, portable, and possesses high practical value. Attached Figure Description

[0012] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0013] Figure 2 This is a top view of the cuvette placement box of this utility model.

[0014] In the diagram: 1. Light shield, 2. Flip cover, 3. Operating table, 4. Main fiber optic cable, 5. Cuvette body, 6. Cuvette placement box, 7. Auxiliary fiber optic cable, 8. Lower receiver, 9. Receiving slot, 10. Waist-shaped spring, 11. Anti-slip pad. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Please see Figure 1-2A micro-volume spectrophotometer cuvette detection structure includes an operating platform 3. A flip cover 2 is rotatably connected to a rotating base on the top of the operating platform 3. The flip cover 2 contains a plane mirror assembly for base detection and an upper receiver, used for the traditional "base mode (micro-volume direct detection)" detection mode. This is a mature existing technology and will not be described in detail here. A light-shielding ring 1 is installed on the flip cover 2. A main optical fiber 4 is provided on the operating platform 3, which can guide the light source into the micro-volume detection structure. A cuvette placement box 6 is also installed at the bottom of the operating platform 3. Inside the cuvette placement box 6 is a cuvette body 5. The cuvette body 5 is a transparent shell structure with a square cross-section, and its side length is... With a diameter of 10mm, the light-shielding ring 1 can block light from above the cuvette body 5 after the flip cover 2 is flipped down, ensuring that the cuvette body 5 is not affected by external stray light during the detection process. The left and right sides of the cuvette placement box 6 are respectively equipped with a lower receiver 8 and an auxiliary optical fiber 7. The lower receiver 8 can receive the light signal passing through the cuvette body 5 and convert it into an electrical signal, which is then transmitted to the computer system. The computer system calculates the concentration and other relevant parameters of the sample based on the cuvette detection algorithm and the received electrical signal, combined with the Lambert-Beer law, and displays the detection results on the operation interface. The auxiliary optical fiber 7 can guide the light source into the detection structure of the cuvette body 5.

[0017] The bottom of the inner wall of the cuvette placement box 6 is provided with a receiving groove 9, and the bottom part of the cuvette body 5 is set inside the receiving groove 9. The receiving groove 9 serves to accommodate and fix the cuvette body 5.

[0018] A waist-shaped spring 10 is symmetrically installed on the inner wall of the cuvette placement box 6. The waist-shaped spring 10 is installed on the inner wall of the cuvette placement box 6 near the opening. When the cuvette body 5 is placed in, it is convenient to squeeze inward. After the bottom end of the cuvette body 5 is inserted into the receiving groove 9, the waist-shaped spring 10 can effectively clamp and fix it, further preventing slippage. An anti-slip pad 11 is adhered to the outer surface of the waist-shaped spring 10. The anti-slip pad 11 can increase the friction when the outer surface of the waist-shaped spring 10 contacts the cuvette body 5.

[0019] A light-transmitting hole is provided on the outside of the cuvette placement box 6, and the position of the light-transmitting hole corresponds to that of the auxiliary optical fiber 7. A condensing lens is also installed on the side wall of the cuvette placement box 6 at the position corresponding to the lower receiver 8. The light emitted through the auxiliary optical fiber 7 can be sent to the lower receiver 8 through the condensing lens and the light-transmitting hole.

[0020] This invention employs a dual-optical-path design, with independent optical paths for the base and cuvette, integrated into a single unit. This eliminates the need for physical replacement of parts. Software selection of either the "base" or "cuvette slot" mode effectively prevents cross-contamination. During cuvette testing, the cuvette is simply placed in the fixed slot next to the base, and the optical path, via the incident fiber, is aligned with the cuvette's light-transmitting aperture. This invention features a simple structure, eliminating the need for a complex optical path switching system. It effectively solves the positioning and light energy loss problems associated with physical switching, improving the measurement range, accuracy, and equipment reliability. It is easy to operate, portable, and possesses high practical value.

[0021] When using the instrument, turn on the micro-volume spectrophotometer and perform a self-test to ensure it is in normal working condition. Use a pipette to draw an appropriate amount of sample and inject it into the cuvette body 5. Place the cuvette body 5 into the cuvette placement box 6 and clamp it with the waist-shaped spring 10. After closing the flip cover 2, the light shield 1 will cover the cuvette body 5, effectively preventing stray light from entering. The stable light source signal emitted by the light source is split into two beams by two optical fibers. One beam of light reaches the cuvette body 5, allowing the light to accurately penetrate the sample inside. The transmitted light signal after irradiating the sample is detected by the receiver 8, which converts the light signal into an electrical signal and transmits it to the computer system. The computer system calculates the sample concentration and other relevant parameters based on the cuvette detection algorithm and the received electrical signal, combined with the Lambert-Beer law, and displays the detection results on the operation interface.

[0022] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cuvette detection structure for an ultra-micro spectrophotometer, comprising an operating stage (3), characterized in that: A flip cover (2) is rotatably connected to the rotating seat at the top of the operating table (3). The operating table (3) is provided with a main optical fiber (4). A cuvette placement box (6) is also installed at the bottom of the operating table (3). The cuvette placement box (6) is provided with a cuvette body (5) inside. A lower receiver (8) and an auxiliary optical fiber (7) are respectively installed on the left and right sides of the cuvette placement box (6).

2. The cuvette detection structure of the ultra-micro spectrophotometer according to claim 1, characterized in that: The bottom of the inner wall of the cuvette placement box (6) is provided with a receiving groove (9), and the bottom part of the cuvette body (5) is located inside the receiving groove (9).

3. The cuvette detection structure for the ultra-micro spectrophotometer according to claim 2, characterized in that: The inner wall of the cuvette placement box (6) is symmetrically equipped with waist-shaped springs (10), and the outer surface of the waist-shaped springs (10) is bonded with anti-slip pads (11).

4. The cuvette detection structure of the ultra-micro spectrophotometer according to claim 3, characterized in that: A light-shielding ring (1) is installed on the flip cover (2).

5. The cuvette detection structure of the ultra-micro spectrophotometer according to claim 4, characterized in that: The cuvette placement box (6) has a light-transmitting hole on its outer side, and the position of the light-transmitting hole corresponds to the auxiliary optical fiber (7).

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