A microalgae culture medium preparation device

Abstract

This utility model relates to the field of microalgae cultivation technology and discloses a microalgae culture medium preparation device, including a laminar flow filter, a constant temperature storage tank, a peristaltic pump, a flow sensor, and a pouring mechanism for conveying culture medium, all connected sequentially via a feeding pipe. The pouring mechanism includes a support frame and a housing slidably mounted on the support frame. A connecting column is movably mounted on one side of the housing, and a fixing block is mounted on the connecting column. A discharge nozzle is detachably mounted on the fixing block. One end of the feeding pipe is connected to the discharge nozzle, which is located above the culture dish. The design of the laminar flow filter creates a sterile positive pressure environment in the nutrient solution pouring area, reducing the risk of culture medium contamination. The design of the pouring mechanism, with the cooperation of a motor and cylinder, drives the discharge nozzle to move in a Z-shape, achieving Z-shaped pouring of the nutrient solution in the culture dish and ensuring uniform distribution of the nutrient solution within the dish.

Description

Technical Field

[0001] This utility model relates to the field of microalgae cultivation technology, specifically to a microalgae culture medium preparation device. Background Technology

[0002] Microalgae cultivation refers to the technology of artificially controlling environmental conditions and utilizing the physiological characteristics of single-celled or simple multicellular microalgae to carry out large-scale and controllable growth and reproduction in specific containers or systems, ultimately obtaining high biomass or high-value products.

[0003] Microalgae culture medium is a solution that provides the necessary nutrients and a suitable environment for microalgae growth. Its composition and ratio need to be optimized according to the characteristics of the algae species, the culture method (photoautotrophic, heterotrophic, or polytrophic), and the target product. The basic nutrients typically contained in microalgae culture medium include: nitrogen source, phosphorus source, potassium source, magnesium source, calcium source, trace elements (iron, manganese, zinc, copper, etc.), and vitamins.

[0004] The traditional microalgae culture medium preparation process is not a closed operation, which has a high risk of human contamination. When pouring the culture medium into the culture dish, the culture medium is prone to accumulate in the culture dish due to the fixed setting of the discharge nozzle, resulting in uneven distribution of the culture medium. Therefore, we need to propose a microalgae culture medium preparation device. Utility Model Content

[0005] The purpose of this invention is to provide a microalgae culture medium preparation device, which, through the design of a filter laminar flow hood, creates a sterile positive pressure environment in the nutrient solution pouring area, reducing the risk of culture medium contamination, thereby solving the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a microalgae culture medium preparation device, comprising a filter laminar flow hood, a constant temperature storage tank, a peristaltic pump, a flow sensor, and a pouring mechanism for conveying culture medium, wherein the pouring mechanism is installed on the side of the filter laminar flow hood;

[0007] The interior of the laminar flow hood is equipped with a feeder for conveying the culture dish. The tilting mechanism includes a support frame and a housing slidably mounted on the support frame. A connecting column is movably mounted on one side of the housing. A fixing block is mounted on the connecting column. A discharge nozzle is detachably mounted on the fixing block. One end of the feeding pipe is connected to the discharge nozzle, and the discharge nozzle is located above the culture dish.

[0008] Preferably, the bottom of the support frame is connected to a connecting frame, which is installed on the side of the filter laminar flow hood.

[0009] Preferably, a cylinder is mounted on the support frame, one end of the piston rod of the cylinder is connected to the back of the housing, and a slide rail is also provided on the support frame, with the housing mounted on the slide rail.

[0010] Preferably, a lead screw is rotatably mounted inside the housing, and a motor that drives the lead screw to rotate is mounted at one end of the housing. The output shaft of the motor passes through the housing and is connected to one end of the lead screw.

[0011] Preferably, the outer wall of the lead screw is threaded with a mounting block, and one end of the connecting column passes through the outer shell and is connected to the mounting block.

[0012] Preferably, one end of the fixing block is provided with an installation groove, and the discharge nozzle is threadedly installed inside the installation groove.

[0013] Preferably, the other end of the fixing block has a through hole communicating with the mounting groove, the end of the discharge nozzle is connected to a connecting pipe, the other end of the connecting column has a notch, one end of the connecting pipe passes through the through hole and is located inside the notch, and is connected to the feeding pipe.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] 1. This utility model achieves a sterile positive pressure environment in the nutrient solution pouring area through the design of a filter laminar flow hood, thereby reducing the risk of culture medium contamination;

[0016] 2. This utility model, through the design of the tilting mechanism, drives the discharge nozzle to move in a Z-shape with the cooperation of the motor and cylinder, realizing the Z-shaped tilting of the nutrient solution in the culture dish, thus ensuring the uniform distribution of the nutrient solution in the culture dish. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the structure of the filter laminar flow hood, feeder, and tilting mechanism of this utility model;

[0019] Figure 3 This is an exploded view of the tilting mechanism of this utility model.

[0020] In the picture:

[0021] 1. Thermostatic storage tank; 2. Peristaltic pump; 3. Flow sensor; 4. Filter laminar flow hood; 5. Feeder; 6. Tilting mechanism; 7. Feeding pipe;

[0022] 61. Support frame; 62. Connecting frame; 63. Cylinder; 64. Housing; 65. Motor; 66. Lead screw; 67. Mounting block; 68. Connecting column; 69. Notch; 610. Fixing block; 611. Mounting groove; 612. Through hole; 613. Discharge nozzle; 614. Connecting pipe. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figure 1-3 This utility model provides a technical solution: a microalgae culture medium preparation device, including a filter laminar flow hood 4, a constant temperature storage tank 1, a peristaltic pump 2, a flow sensor 3, and a pouring mechanism 6 for conveying culture medium, which are connected in sequence through a feeding pipe 7. The pouring mechanism 6 is installed on the side of the filter laminar flow hood 4.

[0025] The thermostatic liquid storage tank 1 is made of high-temperature resistant glass. The top of the thermostatic liquid storage tank 1 is equipped with a sealing cap. The capacity of the thermostatic liquid storage tank 1 is 1-10L. It has a built-in magnetic stirrer to prevent the nutrient solution from settling. The thermostatic liquid storage tank 1 is also equipped with a temperature control module, which adopts PID precise temperature control (30-38℃). The temperature control module has a display screen to show the digital temperature and has an over-temperature alarm function.

[0026] It should be noted that the temperature control module with temperature display and over-temperature alarm functions is existing technology and will not be described in detail here. The temperature control module can be purchased directly from the market.

[0027] The laminar flow filter hood 4 ensures a sterile positive pressure environment when pouring nutrient solution, reducing the risk of contamination.

[0028] The interior of the laminar flow hood 4 is equipped with a feeder 5 for conveying the culture dishes. The feeder 5 is driven by a stepper motor, such as... Figure 3 As shown, the tipping mechanism 6 includes a support frame 61 and a housing 64 slidably mounted on the support frame 61. The support frame 61 is L-shaped.

[0029] The petri dish has a built-in semiconductor cooling plate, which can quickly cool the nutrient solution after pouring, preventing excessive condensation. It is worth noting that an infrared sensing positioning module (not shown in the figure) is installed on the inner top of the filter laminar flow hood 4 to locate the position of the petri dish, ensuring that the petri dish moves below the discharge nozzle 613. The infrared sensing positioning module is existing technology and will not be described in detail here; moreover, the infrared sensing positioning module is commercially available.

[0030] A connecting post 68 is movably installed on one side of the outer shell 64. The connecting post 68 is perpendicular to the outer shell. A strip-shaped through hole is opened on the outer shell 64. The connecting post 68 is movably installed inside the through hole. A fixing block 610 is installed on the connecting post 68. The fixing block 610 is inclined. A discharge nozzle 613 is detachably installed on the fixing block 610. That is, the discharge nozzle 613 is also in an inclined state, which is conducive to the discharge of nutrient solution. The discharge nozzle 613 is a wide-mouth discharge nozzle. One end of the feeding pipe 7 is connected to the discharge nozzle 613. The discharge nozzle 613 is located above the petri dish.

[0031] A connecting frame 62 is connected to the bottom of the support frame 61, and the connecting frame 62 is installed on the side of the filter laminar flow hood 4.

[0032] A cylinder 63 is mounted on the support frame 61. One end of the piston rod of the cylinder 63 is connected to the back of the outer casing 64. A slide rail is also provided on the support frame 61, and the outer casing 64 is mounted on the slide rail. The piston rod of the cylinder 63 drives the extension and return of the outer casing 64. The slide rail ensures the stability and smoothness of the movement of the outer casing 64.

[0033] A lead screw 66 is rotatably mounted inside the outer casing 64. A motor 65, which drives the lead screw 66 to rotate, is mounted at one end of the outer casing 64. The output shaft of the motor 65 passes through the outer casing 64 and is connected to one end of the lead screw 66. The motor 65 is a forward and reverse motor. The motor 65 drives the lead screw 66 to rotate, which in turn drives the mounting block 67 to move the connecting column 68 laterally, which in turn moves the discharge nozzle 613 on the fixed block 610 laterally.

[0034] The outer wall of the lead screw 66 is threaded with a mounting block 67, and one end of the connecting post 68 passes through a through hole on the outer casing 64 and is connected to the mounting block 67.

[0035] One end of the fixing block 610 has an installation groove 611. The shape of the installation groove 611 is designed to fit the discharge nozzle 613. The discharge nozzle 613 is threadedly installed inside the installation groove 611. The discharge nozzle 613 can be disassembled by the threaded engagement between the discharge nozzle 613 and the installation groove 611, making it easy to clean and sterilize the discharge nozzle 613.

[0036] The other end of the fixing block 610 is provided with a through hole 612 that communicates with the mounting groove 611. The end of the discharge nozzle 613 is connected to a connecting pipe 614. The other end of the connecting post 68 is provided with a notch 69. The notch 69 is connected to the through hole 612. One end of the connecting pipe 614 passes through the through hole 612 and is located inside the notch 69. It is connected to the feeding pipe 7. The connection part between the connecting pipe 614 and the feeding pipe 7 is located inside the notch 69.

[0037] When it is necessary to replace the discharge nozzle 613, first disconnect the connection between the connecting pipe 614 and the feeding pipe 7, and then rotate the discharge nozzle 613 to unscrew it out of the mounting slot 611.

[0038] This device also includes a control panel (not shown in the figure), on which programs can be preset, such as: LB agar - 20mL - 50℃. The control panel can also monitor the nutrient solution temperature, flow rate, and amount poured in real time. The preset programs and data monitoring functions of the control panel are common technical means in the current field and are existing technologies, which will not be elaborated here.

[0039] In use, the petri dish is placed on the conveyor belt of the feeder 5 by the feeding robotic arm (not shown in the figure). The magnetic stirrer inside the constant temperature storage tank 1 stirs the nutrient solution to prevent sedimentation. The nutrient solution is transported from the feed pipe 7 to the discharge nozzle 613 by the peristaltic pump 2 and the flow sensor 3, and then poured into the petri dish from the discharge nozzle 613.

[0040] Specifically, when the nutrient solution is discharged from the nozzle 613, it is poured into the interior of the culture dish in a "Z" shape. First, the cylinder 63 pushes the outer shell 64 to move, so that the nozzle 613 moves to the top of the culture dish. Then, the motor 65 drives the lead screw 66 to rotate, which in turn drives the mounting block 67 to move the connecting column 68 laterally (from left to right), which in turn drives the nozzle 613 on the fixing block 610 to move from left to right as well.

[0041] Then, the piston rod of cylinder 63 retracts, motor 65 reverses, pulling the discharge nozzle 613 back while motor 65 drives the discharge nozzle 613 to move from right to left. After it moves into position, cylinder 63 stops running, and motor 65 reverses again, driving the discharge nozzle 613 to move from left to right again, so that the nutrient solution can be poured into the interior of the petri dish in a Z-shape, ensuring the uniformity of the nutrient solution in the petri dish. After the pouring is completed, the feeder 5 continues to transport the petri dish, and then the petri dish can be removed by the unloading robotic arm (not shown in the figure).

[0042] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A microalgae culture medium preparation apparatus, characterized by: Including filter laminar flow hood (4), constant temperature liquid storage tank (1) connected in turn through feeding pipe (7), peristaltic pump (2), flow sensor (3) and pouring mechanism (6) for conveying culture solution, the pouring mechanism (6) is installed on the side of filter laminar flow hood (4); The inside of the filter laminar flow hood (4) is provided with a feeder (5) for conveying culture dishes, the pouring mechanism (6) includes a support frame (61), a housing (64) slidably mounted on the support frame (61), a connecting column (68) movably mounted on one side of the housing (64), a fixed block (610) mounted on the connecting column (68), a discharge nozzle (613) detachably mounted on the fixed block (610), one end of the feeding pipe (7) is connected with the discharge nozzle (613), and the discharge nozzle (613) is located above the culture dish.

2. The microalgae culture medium preparation device according to claim 1, characterized in that: The bottom of the support frame (61) is connected with a connecting frame (62), and the connecting frame (62) is installed on the side of the filter laminar flow hood (4).

3. The microalgae culture medium preparation device according to claim 1, characterized in that: A gas cylinder (63) is mounted on the support frame (61), one end of the piston rod of the gas cylinder (63) is connected to the back of the housing (64), and a slide rail is further arranged on the support frame (61), and the housing (64) is mounted on the slide rail.

4. The microalgae culture medium preparation device according to claim 1, characterized in that: A lead screw (66) is rotatably mounted in the housing (64), and a motor (65) for driving the rotation of the lead screw (66) is mounted at one end of the housing (64), and the output shaft of the motor (65) is connected with one end of the lead screw (66) through the housing (64).

5. The microalgae culture medium preparation device according to claim 4, characterized in that: The outer wall of the lead screw (66) is threadedly connected with a mounting block (67), and one end of the connecting column (68) is connected with the mounting block (67) through the housing (64).

6. The microalgae culture medium preparation device according to claim 5, characterized in that: One end of the fixed block (610) is provided with a mounting groove (611), and the discharge nozzle (613) is threadedly mounted in the mounting groove (611).

7. The microalgae culture medium preparation device according to claim 6, characterized in that: The other end of the fixed block (610) is provided with a through hole (612) in communication with the mounting groove (611), and the distal end of the discharge nozzle (613) is connected with a connecting pipe (614), and the other end surface of the connecting column (68) is provided with a notch (69), and one end of the connecting pipe (614) passes through the through hole (612) and is located in the notch (69), and is connected with the feeding pipe (7).

Images