A control device for culturing chlamydomonas reinhardtii in a constant carbon dioxide

Abstract

The utility model discloses a constant carbon dioxide culture leishnannia culture's controlling means relates to leishnannia field, including culture jar, the vertical rotation is connected with the breather pipe in culture jar, and the breather pipe surface fixedly connected with stirring piece and is provided with a plurality of through -holes, culture jar is fixedly connected with carbon dioxide delivery subassembly, is used for the delivery carbon dioxide to the breather pipe. Advantageous effect lies in: through motor and gear drive, synchronous drive breather pipe rotation and thread cover operation, and carbon dioxide is released with micro -bubble through the breather pipe through -hole, and the breather pipe drives stirring piece rotation, both prolongs the contact time of bubble and algal liquid, and also avoids algal cell settlement, realizes the integration effect of the gas supply just stirring, and promotes the mixing efficiency and gas utilization rate.

Description

Technical Field

[0001] This utility model relates to the field of Chlamydomonas reinhardtii technology, specifically to a control device for culturing Chlamydomonas reinhardtii with constant carbon dioxide. Background Technology

[0002] Chlamydomonas reinhardtii, as a photosynthetic autotrophic model microalga, relies on a stable supply of carbon dioxide for its growth and metabolism. However, in existing cultivation devices, the carbon dioxide supply unit and the algal solution agitation are two independent systems. During gas supply, the gas tends to form large bubbles that rise rapidly, resulting in short contact time with the algal solution and low solubility. During agitation, the bubbles are easily broken, causing gas to escape. The two systems cannot work together, leading to a waste of carbon dioxide and significant local concentration differences in the algal solution, which affects the uniformity of photosynthesis.

[0003] When the carbon source demand of Chlamydomonas reinhardtii increases sharply during its logarithmic growth phase, insufficient gas supply can easily lead to growth stagnation. When the demand decreases during the stationary phase, excessive gas supply can cause a sharp drop in pH inside the tank, disrupting the osmotic pressure of the algal cells and even causing death. Existing devices are cumbersome to operate, with components scattered and requiring separate startup of the gas supply and stirring systems. Furthermore, the tank opening needs to be opened frequently during sampling, making it easy for external bacteria to invade and disrupt the pure culture environment. Utility Model Content

[0004] The purpose of this invention is to provide a control device for cultivating Chlamydomonas reinhardtii with constant carbon dioxide in order to solve the above problems, as detailed below.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] This utility model provides a control device for culturing Chlamydomonas reinhardtii in constant carbon dioxide, including a culture tank, in which a vent pipe is vertically and rotatably connected, and a stirring plate is fixedly connected to the surface of the vent pipe and has several through holes.

[0007] A carbon dioxide delivery assembly is fixedly connected to the culture tank for delivering carbon dioxide into the venting pipe;

[0008] The carbon dioxide delivery assembly delivers carbon dioxide into the vent pipe while simultaneously rotating the vent pipe.

[0009] Using the above-mentioned constant carbon dioxide control device for culturing Chlamydomonas reinhardtii, the seed liquid and culture medium of Chlamydomonas reinhardtii are added through the packing port of the culture tank, and the sealing plug is tightly sealed to prevent contamination and leakage.

[0010] Calibrate the carbon dioxide concentration sensor and pressure sensor, close the exhaust valve, zero the flow meter, and ensure that the motor and cylinder are free from jamming.

[0011] When the motor is started, the ventilation pipe and threaded sleeve are rotated synchronously through the gear two. The rotation of the threaded sleeve drives the screw to drive the piston to reciprocate in the cylinder. When the piston moves upward, the air pressure in the cylinder decreases, one-way valve one opens and one-way valve two closes, and carbon dioxide from the outside is drawn in.

[0012] When the piston moves down, the gas pressure inside the cylinder increases, one check valve closes and the other check valve opens, and carbon dioxide is sent into the vent pipe through the pipeline.

[0013] Carbon dioxide is released as microbubbles from the vent pipe opening. The vent pipe simultaneously drives the stirring plate to rotate, which prolongs the contact time between the bubbles and the algae solution, prevents algae cells from settling, and improves the uniformity of mixing.

[0014] The concentration sensor monitors the concentration in real time. When the concentration is low, the motor speed is increased to increase the carbon dioxide delivery; when the concentration is high, the speed is decreased.

[0015] Preferably, the culture tank is fixedly connected to an exhaust valve, and a packing port is provided at the top of the culture tank, with a sealing plug inside the packing port.

[0016] Preferably, the culture vessel is equipped with a carbon dioxide concentration sensor and a pressure sensor.

[0017] Preferably, the carbon dioxide delivery assembly includes a cylinder fixedly connected to the culture tank, a piston slidably connected inside the cylinder, a threaded sleeve rotatably connected to the top of the cylinder, a lead screw threadedly connected inside the threaded sleeve, the lower end of the lead screw being fixedly connected to the piston, and a one-way valve one and a one-way valve two fixedly connected to the bottom of the cylinder, the one-way valve two being connected to a pipeline through a pipe.

[0018] Preferably, the pipe is a rigid pipe, which is fixedly connected to the cylinder and rotatably connected to the upper end of the vent pipe. The flow directions of the one-way valve and the one-way valve are opposite.

[0019] Preferably, the cylindrical body is a polygonal prism with a polygonal cavity on the inner wall.

[0020] Preferably, a motor is fixedly connected to the culture tank, a second gear is fixedly connected to the output shaft of the motor, and a first gear that meshes with the second gear is fixedly connected to both the vent pipe and the threaded sleeve.

[0021] Preferably, the culture vessel is made of transparent glass.

[0022] Preferably, a flow meter is installed on the pipeline.

[0023] Preferably, the cylinder is made of stainless steel.

[0024] The beneficial effects are:

[0025] 1. Through the motor and gear transmission, the vent pipe is driven to rotate and the threaded sleeve is operated synchronously. Carbon dioxide is released as microbubbles through the vent pipe holes. The vent pipe drives the stirring plate to rotate, which not only prolongs the contact time between the bubbles and the algal liquid, but also avoids the sedimentation of algal cells, achieving the integrated effect of gas supply and stirring, and improving mixing efficiency and gas utilization.

[0026] 2. By monitoring carbon dioxide concentration in real time and adjusting motor speed, the gas supply can be dynamically adapted. When the concentration is low, the speed is increased to increase the delivery volume, and when the concentration is high, the speed is decreased to reduce the delivery volume, ensuring a stable carbon source supply for Chlamydomonas reinhardtii throughout its growth cycle.

[0027] 3. The pressure sensor monitors the gas pressure inside the tank. When the pressure is too high, the exhaust valve is opened to release excess oxygen and undissolved carbon dioxide. After the pressure drops to a safe level, the valve is closed. This not only prevents safety risks caused by excessive gas pressure, but also reduces unnecessary carbon dioxide leakage, ensuring the stability of both the concentration and pressure inside the tank. Attached Figure Description

[0028] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0029] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0030] Figure 2 This is a front view structural diagram of the present invention;

[0031] Figure 3 This is a three-dimensional cross-sectional structural diagram of the present invention;

[0032] Figure 4 This is a schematic diagram of the three-dimensional cross-sectional structure of the cylindrical body of this utility model.

[0033] The annotations in the attached figures are explained as follows:

[0034] 1. Culture tank; 2. Exhaust valve; 3. Packing port; 4. Carbon dioxide concentration sensor; 5. Pressure sensor; 6. Vent pipe; 7. Through hole; 8. Stirring blade; 9. Carbon dioxide delivery assembly; 10. Gear 1; 11. Gear 2; 12. Motor; 13. Cylinder; 14. Pipe; 15. Piston; 16. Lead screw; 17. Threaded sleeve; 18. Check valve 1; 19. Check valve 2; 20. Flow meter. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be described in detail below. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other implementation methods obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0036] See Figures 1-4 As shown, this utility model provides a control device for culturing Chlamydomonas reinhardtii with constant carbon dioxide, including a culture tank 1, a vent pipe 6 vertically rotatably connected inside the culture tank 1, a stirring plate 8 fixedly connected to the surface of the vent pipe 6 and having several through holes 7.

[0037] A carbon dioxide delivery assembly 9 is fixedly connected to the culture tank 1 for delivering carbon dioxide into the venting pipe 6;

[0038] Culture tank 1 is set to 5-50L, 5-10L is commonly used in laboratories, and 20-50L is commonly used in pilot production. The ratio of tank height to inner diameter is 2:1. For example, a 10L tank has an inner diameter of 200mm and a height of 400mm to ensure that the algal solution has sufficient gas contact space.

[0039] The transparent glass is made of high borosilicate glass, which is resistant to high temperatures ≥300℃ and acid and alkali corrosion. The wall thickness is 8-12mm to ensure pressure resistance and can withstand air pressure up to 0.2MPa. The light transmittance of the tank wall is ≥90%, which meets the light requirements of Chlamydomonas reinhardtii photosynthesis and avoids insufficient light from affecting growth.

[0040] The carbon dioxide delivery assembly 9 delivers carbon dioxide into the vent pipe 6 while simultaneously rotating the vent pipe 6. The vent pipe 6 is connected to the top of the culture tank 1 via a mechanical seal of type MG1 with a sealing surface made of silicon carbide graphite. This allows the vent pipe to rotate freely while preventing gas leakage from the tank through the connection gap.

[0041] As an optional implementation, an exhaust valve 2 is fixedly connected to the culture tank 1, and a packing port 3 is opened at the top of the culture tank 1, with a sealing plug installed inside the packing port 3.

[0042] Exhaust valve 2 interface: It is located on the top side wall of the tank body, 20-30mm from the tank opening. The interface specification is G1 / 2 thread. It is connected to the exhaust valve 2, which is a ball valve made of stainless steel with a maximum diameter of 10mm, through threaded sealing. The interface is wrapped with PTFE tape to enhance the sealing performance.

[0043] The culture tank 1 is equipped with a carbon dioxide concentration sensor 4 and a pressure sensor 5.

[0044] Carbon dioxide concentration sensor 4: The infrared sensor model S-100 is selected, with a detection range of 0-5000ppm and an accuracy of ±50ppm. The sensor probe is inserted into the gas phase space inside the tank through the interface on the top of the tank to a depth of 30-50mm. The interface is sealed with double O-rings made of silicone rubber. The sensor output signal is a 4-20mA analog signal, which is convenient for connection with subsequent control systems such as PLC.

[0045] Pressure sensor 5: The diffused silicon pressure sensor model MPX5010 is selected, with a measurement range of 0-0.5MPa and an accuracy of ±0.2%FS. It is installed on the top side wall of the tank, offset from the exhaust valve interface. The probe does not directly contact the algae liquid and monitors the pressure inside the tank indirectly through gas phase pressure, thus avoiding algae liquid contamination of the sensor.

[0046] When the stepper motor 12 is started, its output shaft drives the gear 10 on the vent pipe 6 and the threaded sleeve 17 to rotate synchronously through the gear 2 11. On the one hand, the vent pipe 6 drives the anchor / paddle stirring plate 8 to rotate, providing stirring for the algae liquid; on the other hand, the threaded sleeve 17 drives the lead screw 16 to drive the piston 15 to slide back and forth in the polygonal cylindrical body 13.

[0047] Carbon dioxide delivery: When piston 15 moves upward, the air pressure inside cylinder 13 decreases, and one-way valve 18 opens to draw in external carbon dioxide; when piston moves downward, the air pressure inside cylinder increases, and one-way valve 2 19 opens. Carbon dioxide is sent into venting pipe 6 through 316L stainless steel pipe 14 with flow meter monitoring the flow rate in real time and rotary joint, and then released into algal liquid as 1-2mm microbubbles through through holes 7 on the surface of venting pipe, prolonging the gas contact time.

[0048] Monitoring and Control: Carbon dioxide concentration sensor 4 monitors the concentration inside the tank in real time. If the concentration is too low, the motor speed is increased to increase the gas delivery. If the concentration is too high, the speed is reduced. Pressure sensor 5 monitors the gas pressure inside the tank. If the pressure is too high, the exhaust valve 2 is opened to release pressure. After the pressure drops to a safe value, the valve is closed to balance the gas pressure and concentration and avoid sudden changes in the pH of the algae solution or overpressure in the tank.

[0049] The carbon dioxide delivery assembly 9 includes a cylinder 13 fixedly connected to the culture tank 1, a piston 15 slidably connected inside the cylinder 13, a threaded sleeve 17 rotatably connected to the top of the cylinder 13, a lead screw 16 threadedly connected inside the threaded sleeve 17, the lower end of the lead screw 16 being fixedly connected to the piston 15, and a one-way valve 18 and a one-way valve 19 fixedly connected to the bottom of the cylinder 13, with the one-way valve 19 communicating with the pipe 14 through the pipe 14.

[0050] Pipeline 14 is a rigid pipe, which is fixedly connected to cylinder 13 and rotatably connected to the upper end of vent pipe 6. One-way valve 18 and one-way valve 2 19 have opposite flow directions.

[0051] The cylinder 13 is a polygonal prism with a polygonal cavity on its inner wall.

[0052] A motor 12 is fixedly connected to the culture tank 1. A gear 11 is fixedly connected to the output shaft of the motor 12. A gear 10 that meshes with the gear 11 is fixedly connected to the vent pipe 6 and the threaded sleeve 17. The motor 12 is a stepper motor of model 57HS22 with a power of 100-200W and an adjustable speed range of 50-300rpm. The speed is adjusted by the controller to realize the synchronous adjustment of carbon dioxide delivery and stirring rate. The diameter of the motor output shaft is 12-15mm, and the keyway is machined at the end of the shaft with a specification of 6×6mm to facilitate connection with the gear 11.

[0053] Culture jar 1 is made of transparent glass.

[0054] A flow meter 20 is installed on pipe 14.

[0055] The cylinder 13 is made of stainless steel, and its multi-faceted prism structure is typically a regular hexagonal prism. Made of 304 stainless steel, it boasts high strength and ease of machining. Its inner diameter is 30-50mm, and its length is 150-200mm. The inner wall roughness Ra≤0.8μm reduces piston sliding resistance. The multi-faceted cavity design restricts piston 15 rotation, ensuring that the piston only reciprocates axially, preventing seal failure due to piston rotation.

[0056] A controller is installed on culture tank 1.

[0057] Add seed culture and culture medium to the culture tank and seal it. Start the PLC controller and touch screen, set the carbon dioxide concentration threshold, the pressure range inside the tank and the basic speed of the motor; the PLC automatically calibrates the concentration sensor, pressure sensor and flow meter to ensure real-time data transmission with a sampling frequency of 1 time / second.

[0058] Power and synchronous electronic control adjustment: The PLC sends a start signal to the stepper motor, which drives the air pipe and threaded sleeve to rotate synchronously through gear transmission; the electronic control system can adjust the motor pulse frequency to change the speed, and synchronously adjust the stirring intensity and carbon dioxide supply to avoid manual adjustment lag.

[0059] Electrical control monitoring of conveying flow: The flow meter transmits carbon dioxide flow data to the PLC, which displays it dynamically on the touch screen; when the flow deviates from the set range, the electrical control system automatically analyzes the cause and issues an audible and visual alarm to prompt maintenance to ensure stable conveying.

[0060] Fully automatic monitoring and protection:

[0061] Concentration closed-loop regulation: The concentration sensor transmits data to the PLC, and the deviation is calculated by the PID algorithm. If the concentration is low, the motor speed is increased to increase the gas supply; if the concentration is high, the speed is decreased to reduce the gas supply. The entire process is automatic and the response time is less than 1 second.

[0062] Pressure safety protection: If the air pressure exceeds the upper limit, the PLC controls the exhaust valve to open and release pressure. When the pressure drops to the lower limit, it closes. If the pressure fluctuates more than 5 times in a short period of time, a fault warning will be triggered.

[0063] Abnormal protection: When sensor data is abnormal, the motor will be stopped immediately, the one-way valve will be closed, and the fault code will be displayed on the touch screen for easy troubleshooting.

[0064] Using the above structure, Chlamydomonas reinhardtii seed liquid and culture medium are added through the packing port 3 of culture tank 1, and the sealing plug is tightened to prevent contamination and leakage;

[0065] Calibrate carbon dioxide concentration sensor 4 and pressure sensor 5, close exhaust valve 2, zero flow meter 20, and ensure that motor 12 and cylinder 13 are not stuck.

[0066] Start motor 12, which drives vent pipe 6 and threaded sleeve 17 to rotate synchronously through gear 2 11. The rotation of threaded sleeve 17 drives screw 16 to drive piston 15 to reciprocate in cylinder 13. When piston moves upward, the air pressure in cylinder 13 decreases, one-way valve 18 opens and one-way valve 2 19 closes, and external carbon dioxide is drawn in.

[0067] When the piston moves down, the gas pressure inside the cylinder increases, one-way valve 18 closes and one-way valve 2 19 opens, and carbon dioxide is sent into the vent pipe 6 through pipe 14.

[0068] Carbon dioxide is released as microbubbles from the vent pipe 6 through the hole 7. The vent pipe 6 simultaneously drives the stirring plate 8 to rotate, which prolongs the contact time between the bubbles and the algae liquid, prevents algae cells from settling, and improves the uniformity of mixing.

[0069] Concentration sensor 4 monitors in real time. If the concentration is low, the motor speed 12 is increased to increase the carbon dioxide delivery. If the concentration is high, the speed is decreased.

[0070] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A control device for cultivating Chlamydomonas reinhardtii at a constant carbon dioxide temperature, characterized in that: It includes a culture tank (1), and a vent pipe (6) is vertically rotatably connected inside the culture tank (1). A stirring plate (8) is fixedly connected to the surface of the vent pipe (6) and several through holes (7) are opened. A carbon dioxide delivery assembly (9) is fixedly connected to the culture tank (1) for delivering carbon dioxide into the ventilation pipe (6); The carbon dioxide delivery assembly (9) can rotate the vent pipe (6) while delivering carbon dioxide into the vent pipe (6).

2. The control device for constant carbon dioxide cultivation of Chlamydomonas reinhardtii according to claim 1, characterized in that: An exhaust valve (2) is fixedly connected to the culture tank (1), and a packing port (3) is opened at the top of the culture tank (1), and a sealing plug is provided inside the packing port (3).

3. The control device for constant carbon dioxide cultivation of Chlamydomonas reinhardtii according to claim 1, characterized in that: The culture tank (1) is equipped with a carbon dioxide concentration sensor (4) and a pressure sensor (5).

4. The control device for constant carbon dioxide cultivation of Chlamydomonas reinhardtii according to claim 1, characterized in that: The carbon dioxide delivery assembly (9) includes a cylinder (13) fixedly connected to the culture tank (1), a piston (15) slidably connected inside the cylinder (13), a threaded sleeve (17) rotatably connected to the top of the cylinder (13), a lead screw (16) threadedly connected inside the threaded sleeve (17), the lower end of the lead screw (16) being fixedly connected to the piston (15), and a one-way valve (18) and a one-way valve (19) fixedly connected to the bottom of the cylinder (13), the one-way valve (19) being connected to the pipe (14) through the pipe (14).

5. The control device for constant carbon dioxide cultivation of Chlamydomonas reinhardtii according to claim 4, characterized in that: The pipe (14) is a rigid pipe, and the pipe (14) is fixedly connected to the cylinder (13). The pipe (14) is rotatably connected to the upper end of the vent pipe (6). The flow directions of the one-way valve (18) and the one-way valve (19) are opposite.

6. The control device for constant carbon dioxide cultivation of Chlamydomonas reinhardtii according to claim 4, characterized in that: The cylindrical body (13) is a multi-faceted prism with a multi-faceted cavity on its inner wall.

7. The control device for constant carbon dioxide cultivation of Chlamydomonas reinhardtii according to claim 4, characterized in that: A motor (12) is fixedly connected to the culture tank (1), and a gear two (11) is fixedly connected to the output shaft of the motor (12). A gear one (10) that meshes with the gear two (11) is fixedly connected to both the vent pipe (6) and the threaded sleeve (17).

8. The control device for constant carbon dioxide cultivation of Chlamydomonas reinhardtii according to claim 1, characterized in that: The culture jar (1) is made of transparent glass.

9. The control device for constant carbon dioxide cultivation of Chlamydomonas reinhardtii according to claim 5, characterized in that: A flow meter (20) is installed on the pipe (14).

10. The control device for constant carbon dioxide cultivation of Chlamydomonas reinhardtii according to claim 5, characterized in that: The cylinder (13) is made of stainless steel.

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