Manufacture of polymerase chain amplification kits optimizing an amplification denaturation phase

The digital simulation of PCR processes using matrix differential equations addresses the complexity of PCR kit design by predicting denaturation phases and optimizing amplification parameters, improving the efficiency and accuracy of PCR processes.

WO2025003077A1PCT designated stage expired Publication Date: 2025-01-02BIOMERIEUX SA
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Patent Information

Application Number
PCT/EP2024/067690
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-06-28
Filing Date
2024-06-24
Publication Date
2025-01-02

AI Technical Summary

Technical Problem

The design of polymerase chain reaction (PCR) kits is complex and time-consuming, especially when targeting multiple nucleic acids, as existing methods cannot accurately predict the denaturation phases and hybridization kinetics, limiting the ability to optimize amplification parameters effectively.

Method used

A digital simulation method is proposed that models the PCR process, including hybridization, elongation, and denaturation phases, using matrix differential equations to simulate the concentration dynamics of nucleic acid molecules and primers, allowing for the prediction and optimization of amplification parameters.

Benefits of technology

This approach enables the precise simulation of PCR kinetics, facilitating the design of improved PCR kits by predicting the progress of denaturation phases and selecting optimal parameters for amplification, thereby enhancing the efficiency and accuracy of PCR processes.

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Abstract

Disclosed is a method for digitally simulating a polymerase chain amplification of at least one target, comprising the simulation of a plurality of successive cycles of the amplification, each cycle comprising, sequentially: a hybridization phase; an elongation phase; then a denaturation phase; wherein, for each cycle: the hybridization phase comprises: obtaining, for the cycle, an initial value of a vector of concentrations of a plurality of nucleic acid molecules in the form of single strands; calculating a matrix of concentrations of partial duplexes formed by the hybridization of the plurality of nucleic acid molecules in the form of single strands; the denaturation phase comprises: multiplying a vector of the concentration of elongated duplexes resulting from the application of the elongation phase to the concentrations of duplexes, by an elongated duplex denaturation tensor.
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Citation Information

Patent Citations

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  • Extreme PCR

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