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Application of sugar apple lactone compound V in preparing cancer-treating and anti-cancer medicine

A technology of custard apple lactone and anticancer drug, applied in the field of anticancer drugs, can solve the problems of unsatisfactory curative effect, low curative effect of cancer, large toxic and side effects, etc., and achieve the effect of improving clinical curative effect of anticancer

Inactive Publication Date: 2009-01-14
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

In this patented method extracting Aromatic Lactones (AOL) with strong antitumoral properties against different types of cancers such as lungs or bile duck tissue was found to be more potently cytotoxic compared to other drugs used alone. It also shows better effectiveness when combined with another type of medicine like Fludarabine which targets specific proteins involved in cell division processes.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to use natural products or isolated molecules obtained through extraction methods to treat diseases such as laryngitis caused by viruses like rhinoviris virus, influenza A, etc., while also reducing their harmful impacts over time.

Method used

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  • Application of sugar apple lactone compound V in preparing cancer-treating and anti-cancer medicine
  • Application of sugar apple lactone compound V in preparing cancer-treating and anti-cancer medicine
  • Application of sugar apple lactone compound V in preparing cancer-treating and anti-cancer medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Extraction and preparation method of anemone lactone compound V

[0014] 20 kg of custard apple seeds were crushed into coarse powder, extracted three times with 95% ethanol under reflux, and the ethanol extract was concentrated under normal pressure to obtain 1.2 kg. Sequentially extract 3 times with 10 times the amount of petroleum ether (20-60°C), water-saturated ethyl acetate, and chloroform, and the combined extracts are concentrated and dried to obtain 610 g.

[0015] After repeated silica gel column chromatography, the silica gel particle size is 100-300 mesh, and the mass ratio to the extract is 10:1-20:1; it is eluted with chloroform-methanol gradient with a volume ratio of 100:0-90:10, The collected anemone lactone substance was concentrated and dried to obtain 230g. After several times of silica gel column chromatography, the silica gel particle size is 100-300 mesh, and the mass ratio to the annona lactone substance is 10:1-50:1; the volume ra...

Embodiment 2

[0016] Example 2: Inhibitory effect of annona lactone compound V on transplanted lung cancer

[0017] 1. Experimental animals and tumor strains

[0018] Male healthy C57BL / 6 mice, weighing 18-22 g, aged 6-8 weeks, were purchased from the Animal Experiment Center of our school; mouse Lewis lung cancer tumor strains were purchased from the Animal Experiment Center of Jiangsu Cancer Institute.

[0019] 2. Modeling and experimental methods

[0020] There were 60 C57BL / 6 mice, 10 were randomly reserved in proportion as the negative control group, and the remaining 50 were used for modeling. Prepare Lewis lung cancer tumor-bearing mice, peel off the tumor tissue in the vigorous growth period, and select a well-growing tumor of 1.5mm 3 Left and right, homogenate according to the ratio of tumor (g): normal saline (ml) 1:3, adjust the number of cells to 2×10 6 / ml. Select healthy mice, and inoculate 0.2 ml subcutaneously in the right axilla of each mouse under aseptic conditions. ...

Embodiment 3

[0027] Example 3: Inhibitory effect of annona lactone compound V on transplanted breast cancer

[0028] 2. Experimental animals and tumor strains

[0029] Female healthy TA2 mice, weighing 18-22 g, aged 6-8 weeks, were purchased from the Animal Experiment Center of our school; the mouse breast cancer cell line MA-891 was purchased from the Animal Experiment Center of Jiangsu Cancer Institute.

[0030] 2. Modeling and experimental methods

[0031] There were 60 TA2 mice, 10 were randomly reserved in proportion as the negative control group, and the remaining 50 were used for modeling. Take MA-891 mouse breast cancer cells in logarithmic growth phase cultured in vitro, and adjust the cell concentration to 1×10 7 / ml. Under sterile conditions, MA-891 mouse breast cancer cells were inoculated subcutaneously in the right axilla of TA2 mice, the inoculation volume was 0.2ml / mouse (the number of cells was 2×10 6 / Only). The entire inoculation process must be carried out in a ste...

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Abstract

The invention relates to the new applications of an annonaceous acetogenins compound V, in particular to the applications of the compound V for preparing an anti-cancer drug. Annonaceous acetogenins is extracted from the scratch stock of a custard apple seed with organic solvent, including ethanol, petroleum ether, ethyl acetate and chloroform, etc.; the annonaceous acetogenins is processed by silicagel column chromatography and eluted with chloroform-methanol, petroleum ether-ethyl acetate and ethyl-acetate-methanol; and the annonaceous acetogenins compound V is made. The content of the annonaceous acetogenins compound V is 50 to 90 percent of mass fraction. An endozoic anti-cancer pharmacological test shows that the annonaceous acetogenins compound V has a significant effect on inhibiting the growth of the cancer cells of a lung, a breast and a liver and the anti-cancer activity of the annonaceous acetogenins compound V is higher than chemo-treatment 5-S-Fu, so the annonaceous acetogenins compound V can be used for preparing the anti-cancer drug.

Description

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Claims

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Application Information

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Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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