Method for efficiently preparing (S)-styrene glycol from carbonyl reductase recombinant bacterium

Abstract

The invention discloses a method for efficiently preparing (S)-styrene glycol from a carbonyl reductase recombinant bacterium, belonging to the technical field of biocatalysis asymmetric conversion. The invention provides a novel Candidaparapsiospis carbonyl group reductase gene scr II with Genbank sequence number of GQ411433; the scr II is inserted into a vector pET28a to construct a recombinant plasmid pETSCR II which is converted into E.coli BL21(DE3) competent cell; and a recombinant bacterium E.coli BL21 / pETSCR II with the preservation number of CCTCC NO:M209290 can be obtained by the screening of an LB flat plate containing 100 micrograms / ml kanamycin. The (S)-styrene glycol can be prepared by catalyzing 5g / L 2-carbonyl acetophenone obtained by asymmetrically reducing the recombinant bacterium; and the optical purity of products is 100 percent and the yield reaches 98.1 percent. The invention provides a novel functional gene and an effective path for efficiently preparing the (S)-styrene glycol.

Description

technical field

[0001] Fishing the new carbonyl reductase gene scr II from the genome of Candida parapsilosis, constructing a recombinant strain through genetic engineering, and using it to efficiently prepare (S)-phenylethylene glycol and its application, which belongs to biocatalytic asymmetry transforming technology fields. Background technique

[0002] Optically pure phenylethylene glycol is not only an indispensable and important chiral additive in liquid crystal materials, but also an important intermediate for the preparation of optically active medicines, pesticides and functional materials. meaningful.

[0003] The chemical structure of phenyl glycol is:

[0004] The preparation method of chiral compound includes chemical resolution method, chromatographic resolution method, liquid membrane resolution method or chiral solid membrane resolution membrane resolution method and biological method. Among them, the biological method has mild reaction conditions, singl...