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Triterpenoid saponin compound, application and preparation method
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A technology of triterpene saponins and compounds, applied in the field of triterpene saponins
Inactive Publication Date: 2012-10-24
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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[0006] However, no researchers have reported about the saponins Phaseoloideside D and Phaseoloideside E
Method used
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Embodiment 1 3
[0026] Example 1 Preparation of Triterpenoid Saponins Phaseoloideside D and Phaseoloideside E
[0027] 1. Extraction, separation and purification methods
[0028] Shell the dried twig fruit medicinal material to obtain the twig twig kernel, dry it in the sun, crush and weigh 8000 grams, then soak and extract with 4 times the amount of 70% ethanol at room temperature for 3 times, each time for 24 hours, and concentrate the extract under reduced pressure to obtain semi Transparent extract 518g. Dissolve the extract in a methanol-water mixed solvent with a volume ratio of 9:1, repeatedly extract with an equal volume of petroleum ether until the petroleum ether layer is basically colorless, dissolve the extract in water, and repeatedly extract with an equal volume of ethyl acetate The ethyl acetate layer was basically colorless, and then extracted repeatedly with an equal volume of n-butanol until the n-butanol layer was basically colorless, and the n-butanol layers were combined...
Embodiment 2 3
[0047] Example 2 Preparation of Triterpenoid Saponins Phaseoloideside D and Phaseoloideside E
[0048] Sun-dried the twig seed kernels, crushed and weighed 10,000 grams, leached 7 times with 5 times the amount of 70% ethanol at room temperature, 24 hours each time, concentrated the extract, and then degreased with petroleum ether, and the degreased extract was normalized After alcohol extraction, use ethanol-soluble aqueous layer components, dry under vacuum (dry weight 345 g), redissolve in methanol-water solution with a volume ratio of 9:1, pass through macroporous resin, and elute with water and ethanol gradient , collect 50% ethanol eluate, reclaim ethanol under reduced pressure, obtain 61.61 grams of total saponin extract. The total saponin extract was subjected to silica gel column chromatography, using ethyl acetate: methanol: water as the eluent gradient elution, collecting the fractions containing the triterpene saponins, and going through ODS column chromatography, u...
Embodiment 3
[0049] Example 3 Effects of Phaseoloideside D and Phaseoloideside E on Tumor Cells
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Abstract
The invention provides triterpenoid saponin compounds, applications and preparation methods, relating to a compound Phaseoloideside D and a compound Phaseoloideside E obtained by separating from mimosoideae entada phaseoloides leguminous plants and medical applications as well as preparation methods thereof. The in-vitro anti-tumor experiment indicates that the compound Phaseoloideside D and the compound Phaseoloideside E have stronger inhibiting effect on SHG-44 (human glioma), SW480 (colon cancer cells), HepG-2 (liver cancer cells), Ec-109 (esophageal cancer cells) and MCF-7 (human breast cancer cells), can provide a leading compound for preparing new antineoplastics and has key value to develop and use medicinal resources in China.
Description
technical field [0001] The invention relates to triterpene saponin compounds, uses and preparation methods, in particular to the compounds Phaseoloideside D and Phaseoloideside E isolated from the leguminous plant Mimosaeae Mimosoideae, and their medicinal uses and preparation methods. Background technique [0002] Entada phaseoloides (Linn.) Merr., belongs to Fabaceae Mimosa subfamily Entada genus, evergreen woody large vines, stems twisted, branches glabrous; bipinnate compound leaves, 10-25cm long, There are usually 2 pairs of pinnae, the terminal pair of pinnae become tendrils; leaflets 2-4 pairs, leathery, oblong, 3-8.5cm long, 1.5-4cm wide, apex obtuse, slightly concave, base slightly deviated Oblique, glabrous. It is mainly distributed in Fujian, Guangxi, Yunnan and other places in my country. It mainly contains Kutoamides and triterpene saponins, and has the effect of treating stomach problems, hemorrhoids, and constipation. [0003] Triterpene saponins contained i...
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