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Bubble removing device used for microfluidic channel

A microfluidic channel and bubble technology, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of microfluidic cell culture and analysis experiments, complex device fabrication and experimental operations, The problem of slow removal of air bubbles, etc., achieves the effect of simple structure, easy production and light volume

Active Publication Date: 2013-06-19
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the current microfluidic system debubbling method, such as the slow speed of debubbling, the need to interrupt the liquid flow, and the complexity of device fabrication and experimental operation, and provide a simple, efficient, economical, applicable and stable The unique debubbling device solves the practical problems that have plagued microfluidic cell culture and analysis experiments for a long time

Method used

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  • Bubble removing device used for microfluidic channel
  • Bubble removing device used for microfluidic channel
  • Bubble removing device used for microfluidic channel

Examples

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Embodiment 1

[0040] In this embodiment, the air-removing device provided by the present invention is specifically applied in a microfluidic channel, and the effect of the air-removing device and whether it affects cells cultured in the microfluidic channel are tested.

[0041] For details, see figure 2 ,Will figure 1 The air-removing device with the structure shown is applied in the microfluidic channel, in the state of operation with cells and without cells, where A) is a 24-hour continuous in-situ observation of the cell culture medium flowing through the channel. It can be seen that in No air bubbles appeared during the whole process. B) is 24 hours of continuous in-situ observation of the cell culture medium flowing through the channel where the cells are cultured. It can be seen that no bubbles appear during the whole process, and the state of the cells remains good.

[0042] image 3 Shows the staining situation of the cells in the curved microfluidic channel equipped with the de...

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Abstract

The invention provides a bubble removing device used for a microfluidic channel. The bubble removing device comprises: a cavity positioned at the liquid inlet of a microfluidic channel for buffering a liquid and capturing bubbles; a bubble drainage bypass connected with an upper opening of the cavity for removing bubbles captured by the cavity. And the bubble drainage bypass consists of two bypass pipes and a bypass channel, which is parallel to the microfluidic channel, with the two bypass pipes respectively positioned at two ends of the bypass channel. Specifically, one bypass pipe is used for connecting the bypass channel and the cavity, and the other bypass pipe is used for removing bubbles. The bubble removing device provided in the invention has the advantages of simple structure, high integration degree, no need for support of an external device, and no influence on a normal liquid flow inside the microfluidic channel, thus being suitable for all fluid shear force cell experiments and protein, nucleic acid biochemistry, and immune microfluidic chip detection experiments with a need for no bubble interference in immune microfluidic chips.

Description

technical field [0001] The invention relates to a bubble-removing device for a microfluidic channel and its application. Background technique [0002] The micro-nano analysis system of cell biology is a new generation of cell culture and analysis system developed in recent years. It is an important platform for the study of cell adhesion, differentiation, proliferation behavior and intercellular interaction. Interactions provide unprecedented technological advantages. [0003] Polydimethylsiloxane (PDMS) is a polymer with excellent transparency, low cytotoxicity, and gas permeability. Microfluidic chips based on PDMS and glass slides have been widely used in cell micro-nano Analyze, for example, the differentiation of stem cells, the secretion of neuropeptides in nerve cells, the release and inhibition of single-cell calcium, etc. [0004] However, culturing cells on microfluidic chips faces many problems, including bacterial contamination, liquid leakage, pipe blockage, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
CPCC12M23/16C12M29/20
Inventor 蒋兴宇郑文富张伟王卓
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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