Cell culture composition and use for primary culture of tumor cells

Abstract

The invention provides a cell culture composition and application for primary culture of tumor cells. In the use of the cell culture composition of the present invention in the primary culture of epithelial-derived tumor cells, the cell culture composition comprises a serum-free medium for keratinocytes and a serum-free additive for nerve cell culture, and the cells No serum was included in the culture composition. The cell culture composition of the present invention can successfully perform primary culture of epithelial-derived tumor cells in vitro, greatly improves the success rate of primary culture of epithelial-derived tumor cells, and can inhibit the growth of mesenchymal cells in cancer tissues. For the growth of fibroblasts and endothelial cells, for example, the application of the cell culture composition of the present invention can make the primary culture of epithelial-derived tumor cells successful within 10 days, and the success rate of culture is mostly above 80%; and basically can Stable passage to more than 8 generations can successfully establish a line.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cell culture composition for primary culture of tumor cells, its application, and a primary culture method. Background technique [0002] In the current tumor cell culture technology, the most commonly used technology for primary culture and line establishment of tumor cells is to use a basal medium supplemented with 10% fetal calf serum (FCS for short) to cultivate tumor cells. However, the current application of this tumor cell culture technology to cultivate primary tumor cells has a very low success rate. For example, the success rate of primary lung cancer and prostate cancer cells cultured with 10% FCS+RPMI1640 is lower than 10%, and the success rate of subculture and line establishment is even lower. Therefore, currently established cell lines are mostly used in tumor research. Since most of the established cell lines have been cultured and passaged in vitro fo...

AI Technical Summary

Problems solved by technology

However, the success rate of culturing primary cancer cells in serum-free or low-serum medium is still very low, most of which do not exceed 20%, and the culture time usually needs to be more than 1 month

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