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Mink canine distemper-parvovirus enteritis dual live vaccine and its preparation method and application

A technology for mink parvovirus and canine distemper virus, which is applied in the directions of antiviral agents, pharmaceutical formulations, and medical preparations containing active ingredients, etc., can solve the problems of increasing breeding costs, difficult transportation and storage, and sudden death of minks.

Active Publication Date: 2016-08-24
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the mink distemper and parvovirus enteritis vaccines that are popular in the domestic market are single vaccines that have not been freeze-dried, and it is often necessary to immunize mink twice or more, which not only increases the cost of breeding, but also increases the cost of farmers. workload, and cause severe stress response to the herd, and even lead to sudden death of individual mink
At the same time, wet seedlings also have the disadvantages of short shelf life, difficult transportation and storage, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Passage weakening of mink parvovirus MEVB

[0022] 1. Passage weakening of mink parvovirus MEVB

[0023] (1) The mink parvovirus vaccine strain MEVB was inoculated on cat kidney cells CRFK by synchronous inoculation for virus amplification and culture, and the cell lesion was observed every day. When the cell lesion reached about 80%, it could be frozen and thawed repeatedly. The virus was harvested using the method described above and labeled MEVB-F1 (passage 1).

[0024] (2) The first-generation virus MEVB-F1 was inoculated and harvested according to the method described above, and the virus was labeled as MEVB-F2 (second generation). Using the same method, the virus was passaged to passage 61, labeled as MEVB-F61.

[0025] (3) When the mink parvovirus MEVB was continuously passaged to the 10th generation (MEVB-F10), the virus was cloned and plaque-purified. The method is: dilute the virus to be purified 10 times to 10 -7 , take 10 -3 ~10 -7 0.1ml of v...

Embodiment 2

[0034] The preparation of embodiment 2 mink canine distemper-parvovirus enteritis dual live vaccine

[0035] 1. Optimizing the ratio of canine distemper virus and parvovirus in the dual vaccine

[0036] (1) Dilute the prepared attenuated strain MEVB-F61 to: 10 4.5 TCID 50 / ml, 10 5.0 TCID 50 / ml, 10 5.5 TCID 50 / ml and 10 6.0 TCID 50 / ml, hindlimb muscle inoculated negative mink, 1ml each, and a non-inoculated control group was set at the same time. 6 months after immunization, the minks of each group were challenged with parvovirus testing virulence, and inoculated with 10ml of 100 LD by intragastric administration 50 (half lethal dose). The body temperature, appetite, mental state and other physiological indicators and death conditions of minks attacked with poison were observed continuously for 14 days. The results showed that the protection rates of inoculation with different titers of virus were: 85%, 90%, 95% and 95%.

[0037] (2) Dilute the prepared attenuate...

Embodiment 3

[0046] Example 3 Safety Test of Mink Distemper-Parvovirus Enteritis Dual Live Vaccine

[0047] Get qualified mink canine distemper-parvovirus enteritis dual live vaccine 3 batches (prepared by the method of embodiment 2), hindlimb muscle inoculation antibody negative mink, set single dose (canine distemper virus titer ≥ 10 4.0 / head portion, mink parvovirus titer ≥ 10 5.5 / head portion), single-dose repetition and super-dose (10 times) immunization groups, and non-vaccinated minks were set as controls. After vaccination, the body temperature, appetite, mental state and other physiological indicators of the minks were observed for 14 consecutive days, and the lesions of the main organs of the vaccinated minks were observed by autopsy. The test results showed that after the single-dose, repeated single-dose and over-dose inoculation, all the physiological indexes of the mink were normal, without showing any clinical symptoms, and the main organs of the inoculated mink had no p...

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PUM

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Abstract

The invention discloses a mink canine distemper-parvovirus enteritis bigeminal live vaccine as well as a preparation method and application thereof. The bigeminal live vaccine comprises the effective constituents of a mink parvovirus attenuated vaccine strain MEVB-F61 and a mink canine distemper attenuated vaccine strain CDV3-CL, wherein the artificially-attenuated mink parvovirus attenuated vaccine strain MEVB-F61 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the mink parvovirus attenuated vaccine strain MEVB-F61 is CGMCC No.9560. Safety testing results show that no any adverse effect is realized when the mink is inoculated with the bigeminal live vaccine; an effect test result shows that after inoculation of the single-dose bigeminal live vaccine, the minks are prevented from attach of high toxicity for detecting canine distemper virus and parvovirus, so that minks are protected. The bigeminal live vaccine provided by the invention can simultaneously prevent two diseases of mink canine distemper and mink parvovirus enteritis, that two diseases are prevented through an injection of the bigeminal live vaccine is realized, and the bigeminal live vaccine has a wide application prospect.

Description

technical field [0001] The invention relates to a dual live vaccine and its preparation method and application, in particular to a mink distemper-parvoviral enteritis dual live vaccine and its preparation method and application. The invention belongs to the technical field of veterinary biological products. Background technique [0002] Canine distemper is an acute, febrile, highly contagious infectious disease caused by canine distemper virus belonging to the genus Morbillivirus in the Paramyxoviridae family. Other pneumonia, gastroenteritis, and occasional neurological symptoms are prone to secondary mixed infection and secondary infection of other bacteria and viruses, and the mortality rate can be as high as 80%. Virus-carrying animals are the most dangerous source of infection. Viruses are excreted through eye and nasal secretions, saliva, urine, and feces, and are mainly transmitted through the digestive tract and respiratory tract. It is one of the three major disease...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/23A61P31/14A61P31/20
Inventor 闫喜军胡博白雪赵建军张蕾张海玲柴秀丽刘昊
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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