A synergistic pharmaceutical composition for treating diabetes

A composition, diabetes technology, used in drug combinations, medical raw materials derived from mollusks, metabolic diseases, etc.

Active Publication Date: 2019-09-13
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research report on the synergistic effect of total saponins of Anemarrhena and clam polysaccharide composition has not been seen yet

Method used

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  • A synergistic pharmaceutical composition for treating diabetes
  • A synergistic pharmaceutical composition for treating diabetes
  • A synergistic pharmaceutical composition for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Effect of the composition of total saponins of Anemarrhena and clam polysaccharide on cell viability and NO secretion in high glucose-induced HUVEC injury model

[0053] In this experiment, the method of simultaneous modeling and drug administration was adopted. The specific method is as follows:

[0054] When the cells grow to 90% confluence in the culture flask, pour off the medium and wash twice with PBS; digest the cells with 0.25% trypsin for about 1 min, pour off the trypsin, and add medium containing 10% serum to stop the digestion , discard the medium, add 4mL of new medium containing 10% FBS, gently pipette the cells; count the cells, dilute the cells to 3×10 4 cells / mL, inoculated in 96-well plate, inoculated 200 μL per well.

[0055] Place the 96-well plate after seeding the cells in 5% CO 2 . Culture in a constant temperature incubator at 37°C. When the cells grow to 90% confluence, replace with low-sugar DMEM medium containing 1% (v / v) FBS and ...

Embodiment 2

[0068] Example 2: Effect experiment of the composition of total saponins of Anemarrhena and clam polysaccharides on insulin secretion of RIN cells

[0069] Grouping and administration: cells in the logarithmic growth phase were digested with 0.25% trypsin and blown into a single cell suspension, counted on a hemocytometer, and RPM1640 culture medium was added to adjust the concentration of cells to 1×10 5 cells / mL or so. Add the adjusted concentration of cells into a 96-well culture plate, 200 μL per well, divide the cells into blank control group, positive control group and 1-13 sample administration group, set up 6 parallel wells in each group, and place the cells in 37 °C, 5% CO 2 cultured in a saturated humidity incubator.

[0070] After the cells were cultured for 24 hours, the original culture solution was sucked and discarded. The blank control group was added with new cell culture solution. 200 μL of cell culture solution of different sample liquids. After continui...

Embodiment 3

[0086] Example 3: Effect experiment of total saponins of Anemarrhena and clam polysaccharide composition on glucose consumption of palmitic acid-induced HepG2 cell insulin resistance model

[0087] Modeling and administration: When the cells are incubated in the culture flask until they are 80%-90% confluent, discard the medium and wash twice with PBS; digest the cells with 0.25% trypsin for about 1 min, pour out the trypsin, and add The medium containing 10% serum stopped the digestion, poured out the medium, added a new medium containing 10% FBS, gently blown the cells; counted the cells, and diluted the cells to 8×10 4 cells / mL, inoculated in a 96-well plate, inoculated 200 μL per well, and added 200 μL of D-H timosaponin nk's solution to each well around it.

[0088] When the cells in the 96-well plate reached 80% confluence, the induction solution was added for modeling. The specific operation method is to suck out the medium in each well, and then add different medium, ...

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Abstract

The invention discloses a synergistic medicine composition for treating diabetes. Rhizoma anemarrhenae total saponins and mactra veneriformis polysaccharide serve as main effective ingredients of the composition. It is found for the first time that compared with rhizoma anemarrhenae total saponins and mactra veneriformis polysaccharide, the rhizoma anemarrhenae total saponins and mactra veneriformis polysaccharide composition expresses good blood sugar reducing synergism in vitro and vivo.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a synergistic pharmaceutical composition for treating diabetes. The composition uses total timosaponins and clam polysaccharide as main active ingredients. Background technique [0002] Diabetes, known as diabetes in ancient times, means weight loss and polydipsia. Modern medicine has found that it is a common endocrine disease. It is caused by the absolute or relative lack of insulin in the human body, which causes the blood glucose concentration to rise, and then a large amount of sugar is released from the urine. Excreted, and symptoms such as polydipsia, polyuria, polyphagia, weight loss, dizziness, and fatigue appear. Further development then causes various serious acute and chronic complications throughout the body, threatening health. Diabetes is usually divided into type 1 diabetes and type 2 diabetes. Type II diabetes accounts for the majority o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8964A61P3/10A61K31/715A61K35/618
Inventor 吴皓杨小林
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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