Synergistic medicine composition for treating diabetes

A composition, diabetes technology, used in drug combinations, medical raw materials derived from mollusks, metabolic diseases, etc.

Active Publication Date: 2016-03-16
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research report on the synergistic effect of total saponins of A

Method used

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  • Synergistic medicine composition for treating diabetes
  • Synergistic medicine composition for treating diabetes
  • Synergistic medicine composition for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: The effect experiment of the composition of the total saponins of Anemarrhena vulgaris and the polysaccharide of four-cornered clam on the cell viability and NO secretion of HUVEC injury model induced by high glucose

[0053] In this experiment, the method of modeling and drug delivery was used at the same time. The specific methods are as follows:

[0054] After incubating the cells in the culture flask to 90% confluence, discard the medium and wash twice with PBS; digest the cells with 0.25% trypsin for about 1 min, discard the trypsin, and add 10% serum-containing medium to terminate the digestion , Pour out the medium, add 4mL of new medium containing 10% FBS, gently pipette the cells; perform cell count, dilute the cells to 3×10 4 cells / mL, seeded in 96-well plate, seeded 200μL per well.

[0055] Place the 96-well plate after seeding the cells in 5% CO 2 , Cultivate in a constant temperature incubator at 37°C. When the cells grow to 90% confluence, change to a ...

Embodiment 2

[0068] Example 2: Experiment on the effect of the composition of the total saponins of Anemarrhena vulgaris and the polysaccharides of tetracornis clam on insulin secretion in RIN cells

[0069] Grouping and administration: cells in the logarithmic growth phase are digested with 0.25% trypsin and then pipetted into a single cell suspension, counted on a hemocytometer, add RPM1640 culture medium to adjust the cell concentration to 1×10 5 About cells / mL. Add the adjusted concentration of cells to a 96-well culture plate, 200μL per well, divide the cells into a blank control group, a positive control group and 1-13 sample administration groups. Each group has 6 parallel wells, and the cells are placed in 37 ℃、5%CO 2 Cultivate in an incubator with saturated humidity.

[0070] After cell culture for 24 hours, the original culture medium was aspirated, the blank control group was added with new cell culture medium, the positive control group was added with the culture medium containing 1...

Embodiment 3

[0086] Example 3: The effect of the composition of Zhimu total saponins and four-cornered clam polysaccharide on glucose consumption of palmitic acid-induced HepG2 cell insulin resistance model

[0087] Modeling and administration: When the cells in the culture flask grow to 80%-90% confluence, discard the culture medium and wash twice with PBS; digest the cells with 0.25% trypsin for about 1 min, discard the trypsin, and add Stop the digestion with the medium containing 10% serum, discard the medium, add new medium containing 10% FBS, and gently pipette the cells; perform the cell count and dilute the cells to 8×10 4 Cells / mL, seeded in a 96-well plate, seeded with 200μL per well, and added 200μL of D-H Anemarrhena nk's solution to each surrounding well.

[0088] When the cells in the 96-well plate grow to 80% confluence, add induction solution for modeling. The specific operation method is to aspirate the culture medium in each well, and then add different culture medium, liquid ...

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Abstract

The invention discloses a synergistic medicine composition for treating diabetes. Rhizoma anemarrhenae total saponins and mactra veneriformis polysaccharide serve as main effective ingredients of the composition. It is found for the first time that compared with rhizoma anemarrhenae total saponins and mactra veneriformis polysaccharide, the rhizoma anemarrhenae total saponins and mactra veneriformis polysaccharide composition expresses good blood sugar reducing synergism in vitro and vivo.

Description

Technical field [0001] The invention belongs to the field of traditional Chinese medicine, and specifically relates to a synergistic pharmaceutical composition for the treatment of diabetes. The composition uses the total saponins of Anemarrhena and the polysaccharide of four-cornered clam as main effective ingredients. Background technique [0002] Diabetes, the ancient name of diabetes, which means weight loss and polydipsia. Modern medicine has found that it is a common endocrine disease. It is caused by the absolute or relative lack of insulin in the human body. It is excreted in the middle, and symptoms such as polydipsia, polyuria, polyphagia, weight loss, dizziness, and fatigue appear. Further development will cause various serious acute and chronic complications throughout the body and threaten health. Diabetes is usually divided into type 1 diabetes and type 2 diabetes. Among diabetic patients, type II diabetes accounts for the majority. If diabetic patients are not w...

Claims

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Application Information

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IPC IPC(8): A61K36/8964A61P3/10A61K31/715A61K35/618
Inventor 吴皓杨小林
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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