Mycelium active substance of cicadae and its composition for protecting nerve cells

Abstract

The invention discloses cordyceps cicadae / cordyceps sobolifera mycelium active substance and a composition for protecting nerve cells. Under the noncytotoxic concentration of 40 mu / ml, the cordyceps cicadae / cordyceps sobolifera mycelium active substance can be used for protecting the neurotoxicity of MPP+ to the dopaminergic neuron, and also can be used for inhibiting the cell apoptosis caused by the pressure of the endoplasmic reticulum caused by tunicamycin, and therefore, the cordyceps cicadae / cordyceps sobolifera mycelium active substance has the potential of preventing neurodegenerative diseases.

Description

technical field

[0001] The invention relates to mycelium active substances, in particular to cicada flower mycelium active substances and a composition for protecting nerve cells. Background technique

[0002] Neurodegenerative Diseases:

[0003] Neurodegenerative diseases are symptoms in which neurons in the brain and central nervous system gradually lose function. The brain and spinal cord are composed of various neurons, each responsible for different functions, such as controlling movement, processing and receiving information, and making judgments. The hippocampus in the brain is mainly the hub responsible for learning and memory. Damage to nerve cells in the hippocampus can lead to dementia and learning defects. Neurodegenerative diseases can result from the deterioration of neurons or the loss of function of the myelin sheath that transmits messages over time. Because the nerve cells in the brain and spinal cord are not easy to regenerate, as long as they are damag...

Examples

Embodiment 1

[0034] (1) The source of the organism

[0035] The big cicada flower (Cordyceps cicadae) mycelium used in the present embodiment can be purchased from the big cicada flower (Cordyceps cicadae) mycelium BCRC MU30106 (deposit date: 2013) of the Food Industry Development Research Institute deposited in Taiwan, China On November 25th); the little cicada flower (Cordyceps sobolifera) mycelium system used can be purchased from the little cicada flower (Cordyceps sobolifera) mycelium BCRC37801 (deposit date: March 12, 2010), but the active substance of the cicadae mycelium described in the present invention is not limited to be obtained by this strain.

[0036] (2) Fermentation of organisms

[0037] The liquid culture of cicadae mycelium active substance comprises that cicadae mycelium is inoculated on the plate, uses potato dextrin medium (Potato Dextroso Agar, PDA) at suitable temperature such as 15-35 ℃ (preferably about 25 After culturing for 5 days to two weeks at ℃), the myce...

Embodiment 2

[0044] Analysis of the neurotoxic injury and protection of nerve cells by the active substance of the cicadae mycelium described in Example 1.

[0045] 1. Antagonize MPP + NG108-15 cell line toxicity

[0046] Cell lines of NG108-15 were laid at a density of 2×10 4 cells / ml-well 96-well flat-bottomed tissue culture dish; after 24 hours, each cell was properly pasted on it; finally, these cells were tested with a test sample (active substance of cicadae mycelium). The detection samples used dimethyl sulfoxide (DMSO) as a solvent, and were dissolved in DMSO at concentrations of 10, 20 and 40 mM, respectively. The concentration of DMSO in the medium (medium) was maintained at no more than 1 μl / ml to ensure that it would not affect the growth of NG108-15 cells; after the cells were treated with the test samples for 2 days, the concentrations of 20, 40, and 60 μM MPP were respectively + (1-methyl-4-phenylpyridine) treated NG108-15 cells; four days later, remove the medium and add...

technical field

[0001] The invention relates to mycelium active substances, in particular to cicada flower mycelium active substances and a composition for protecting nerve cells. Background technique

[0002] Neurodegenerative Diseases:

[0003] Neurodegenerative diseases are symptoms in which neurons in the brain and central nervous system gradually lose function. The brain and spinal cord are composed of various neurons, each responsible for different functions, such as controlling movement, processing and receiving information, and making judgments. The hippocampus in the brain is mainly the hub responsible for learning and memory. Damage to nerve cells in the hippocampus can lead to dementia and learning defects. Neurodegenerative diseases can result from the deterioration of neurons or the loss of function of the myelin sheath that transmits messages over time. Because the nerve cells in the brain and spinal cord are not easy to regenerate, as long as they are damag...

Examples

Embodiment 1

[0034] (1) The source of the organism

[0035] The big cicada flower (Cordyceps cicadae) mycelium used in the present embodiment can be purchased from the big cicada flower (Cordyceps cicadae) mycelium BCRC MU30106 (deposit date: 2013) of the Food Industry Development Research Institute deposited in Taiwan, China On November 25th); the little cicada flower (Cordyceps sobolifera) mycelium system used can be purchased from the little cicada flower (Cordyceps sobolifera) mycelium BCRC37801 (deposit date: March 12, 2010), but the active substance of the cicadae mycelium described in the present invention is not limited to be obtained by this strain.

[0036] (2) Fermentation of organisms

[0037] The liquid culture of cicadae mycelium active substance comprises that cicadae mycelium is inoculated on the plate, uses potato dextrin medium (Potato Dextroso Agar, PDA) at suitable temperature such as 15-35 ℃ (preferably about 25 After culturing for 5 days to two weeks at ℃), the myce...

Embodiment 2

[0044] Analysis of the neurotoxic injury and protection of nerve cells by the active substance of the cicadae mycelium described in Example 1.

[0045] 1. Antagonize MPP + NG108-15 cell line toxicity

[0046] Cell lines of NG108-15 were laid at a density of 2×10 4 cells / ml-well 96-well flat-bottomed tissue culture dish; after 24 hours, each cell was properly pasted on it; finally, these cells were tested with a test sample (active substance of cicadae mycelium). The detection samples used dimethyl sulfoxide (DMSO) as a solvent, and were dissolved in DMSO at concentrations of 10, 20 and 40 mM, respectively. The concentration of DMSO in the medium (medium) was maintained at no more than 1 μl / ml to ensure that it would not affect the growth of NG108-15 cells; after the cells were treated with the test samples for 2 days, the concentrations of 20, 40, and 60 μM MPP were respectively + (1-methyl-4-phenylpyridine) treated NG108-15 cells; four days later, remove the medium and add...

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