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Use of small-dose propofol in the preparation of products for preventing and treating post-traumatic stress disorder

A technology for post-traumatic stress and acute stress disorder, applied in the fields of application, function of food ingredients, anti-toxins, etc., can solve problems such as unseen propofol

Inactive Publication Date: 2018-08-10
THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there are no relevant reports on the use of propofol in the prevention and treatment of PTSD

Method used

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  • Use of small-dose propofol in the preparation of products for preventing and treating post-traumatic stress disorder
  • Use of small-dose propofol in the preparation of products for preventing and treating post-traumatic stress disorder
  • Use of small-dose propofol in the preparation of products for preventing and treating post-traumatic stress disorder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment one: the effect of propofol in TDS model rat

[0040] 1. Main method

[0041] 1. Experimental device:

[0042] Electric stimulation claustrophobic box (Med company, model: MED-VFC-NIR-M)

[0043] spontaneous activity box

[0044] elevated plus maze

[0045] Water maze test box

[0046] mouse ladder

[0047] Victor3 multifunctional microplate reader (Perkin Elmer, USA; model: 2104)

[0048] Electronic analytical balance (Germany Sartrious company)

[0049] Carbon dioxide incubator (Napco, USA; model: 5410)

[0050]Cell ultra-clean workbench (Beijing Changping Semiconductor Equipment Factory No. 1; model: JJT-1300)

[0051] Centrifuge (Hunan Xiangyi Laboratory Instrument Development Co., Ltd.; model: TDZ4-WS)

[0052] Inverted microscope (Chongqing Optical Instrument Factory; model: S2-D2 )

[0053] Constant temperature oscillator (Taicang Experimental Equipment Factory; model: THZ-C)

[0054] Electric constant temperature water bath (Beijing Changfe...

Embodiment 2

[0105] 6. The expression of P-CREB and BDNF was measured by western blotting in the hippocampal brain region of TDS rats. Compared with the control group, P-CREB in the hippocampal brain region of TDS rats (see Figure 1-10 , * indicates that compared with the blank control group, statistical P value Figure 1-11, * indicates that compared with the blank control group (statistical P value Figure 1-10 , # indicates that the statistical P value compared with the model group Figure 1-11 , # indicates that the statistical P value compared with the model group <0.05, ## indicates that the statistical P value compared with the model group <0.01) expression, indicating that propofol plays an important role in the plastic regulation of neuron synapses Brain-derived neurotrophic factor (BDNF) in the brain plays a role in alleviating stress-induced regeneration impairment of hippocampal neurons. Example two: the effect of propofol on the foot-shock model

[0106] 1. Main method

[01...

Embodiment 3

[0154] Example 3. Effect of Propofol on NO Content in Glioma Cells

[0155] (1) Main method

[0156] 1. Drug configuration

[0157] (1) Propofol: Propofol: 10mg / ml, that is, 0.01mg / ul, take 4ul of propofol and dilute to 200ul to be 0.0002mg / ul (A) (the following is the dose for one well).

[0158] (2) S-Nitrosoacetylpenicillamine (SNAP): Add 1.25ml of dimethyl sulfoxide to 25mg of SNAP to aid dissolution, and then add 1.25ml of water (A0.01mg / ul).

[0159] (3) 2.7-dichlorohydrofluorescein (DCFH-DA): a concentration of 0.1mmol / l is formulated, and the total volume in each well is 101ul. According to the formula mmol / L=mg / M, each The dose of DCFH-DA in the well is 0.0049216 mg, if these doses are obtained in 1 ul, the concentration of DCFH-DA should be 4.9216 mg / ml, so 5 mg of DCFH-DA was weighed and dissolved in methanol.

[0160] 2. Determination of Propofol on NO Production in Glioma Cells

[0161] After the cells were plated, change the medium (containing 2.0, 6.0, 8.0, an...

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Abstract

The invention discloses application of a small dose of propofol in preparing products for preventing and curing post-traumatic stress disorder (PTSD). Stress disorder and its related diseases include acute stress disorder (ASD) and PTSD. According to the test of injecting a small dose of propofol in the abdominal cavity of a PTSD pattern animal, the small dose of propofol has function of relieving fear, anxiety and learning and memory impairment of the PTSD pattern animal, and can decrease expressions of inducible nitric oxide synthase (iNOS) and neurons nitric oxide synthase (nNOS) which are caused by strong stress, increase expression of brain derived neurotrophic factor (BDNF) which plays an important role in neuroplasticity regulation and reduce release of nitric oxide (NO) of glioma cell of the pattern animal greatly. Accordingly, propofol with the advantages of quick action, quick metabolic clearance and no residues has good application prospect.

Description

technical field [0001] The present invention relates to the application of propofol in stress disorder and related diseases thereof, in particular to the application of low-dose propofol in the preparation of prevention, alleviation and / or treatment of post-traumatic stress disorder syndrome (Post-traumatic stress disorder, PTSD ) use in medicine. Background technique [0002] PTSD refers to a mental disorder that appears delayed and persists for a long time after an individual experiences or witnesses a major traumatic event. Associative stimulus. [0003] PTSD was first discovered in war veterans, but serious natural disasters, terrorist attacks, major traffic accidents, violent personal attacks, and major epidemics can all lead to the occurrence of PTSD. The epidemiological survey in 2010 showed that the incidence rate of PTSD in the United States was 7-8%, and the incidence rate in active servicemen of the US military was 14-16%. The incidence rate of PTSD in 260 orph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/05A61P39/00A61P25/00A23L33/00
CPCA23V2002/00A61K31/05A23V2200/322A23V2200/31
Inventor 王恒林刘怀霞张有志张黎明
Owner THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
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