Application of small dose of propofol in preparing products for preventing and curing post-traumatic stress disorder (PTSD)
A post-traumatic stress, low-dose technology, applied in the application, function of food ingredients, antidote, etc., can solve problems such as unseen propofol
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Embodiment 1
[0039] Embodiment one: the effect of propofol in TDS model rat
[0040] 1. Main method
[0041] 1. Experimental device:
[0042] Electric stimulation claustrophobic box (Med company, model: MED-VFC-NIR-M)
[0043] spontaneous activity box
[0044] elevated plus maze
[0045] Water maze test box
[0046] mouse ladder
[0047] Victor3 multifunctional microplate reader (Perkin Elmer, USA; model: 2104)
[0048] Electronic analytical balance (Germany Sartrious company)
[0049] Carbon dioxide incubator (Napco, USA; model: 5410)
[0050]Cell ultra-clean workbench (Beijing Changping Semiconductor Equipment Factory No. 1; model: JJT-1300)
[0051] Centrifuge (Hunan Xiangyi Laboratory Instrument Development Co., Ltd.; model: TDZ4-WS)
[0052] Inverted microscope (Chongqing Optical Instrument Factory; model: S2-D2 )
[0053] Constant temperature oscillator (Taicang Experimental Equipment Factory; model: THZ-C)
[0054] Electric constant temperature water bath (Beijing Changfe...
Embodiment 2
[0105] 6. The expression of P-CREB and BDNF was measured by western blotting in the hippocampal brain region of TDS rats. Compared with the control group, P-CREB in the hippocampal brain region of TDS rats (see Figure 1-10 , * means statistical P value Figure 1-11, * indicates that compared with the blank control group (statistical P value Figure 1-10 , # indicates that the statistical P value compared with the model group Figure 1-11 , # indicates that the statistical P value compared with the model group <0.05, ## indicates that the statistical P value compared with the model group <0.01) expression, indicating that propofol plays an important role in the plastic regulation of neuron synapses Brain-derived neurotrophic factor (BDNF) in the brain plays a role in alleviating stress-induced regeneration impairment of hippocampal neurons. Example two: the effect of propofol on the foot-shock model
[0106] 1. Main method
[0107] 1. Experimental setup
[0108] Electric stim...
Embodiment 3
[0154] Example 3. Effect of Propofol on NO Content in Glioma Cells
[0155] (1) Main method
[0156] 1. Drug configuration
[0157] (1) Propofol: Propofol: 10mg / ml, that is, 0.01mg / ul, take 4ul of propofol and dilute to 200ul to be 0.0002mg / ul (A) (the following is the dose for one well).
[0158] (2) S-Nitrosoacetylpenicillamine (SNAP): Add 1.25ml of dimethyl sulfoxide to 25mg of SNAP to aid dissolution, and then add 1.25ml of water (A0.01mg / ul).
[0159] (3) 2.7-dichlorohydrofluorescein (DCFH-DA): a concentration of 0.1mmol / l is formulated, and the total volume in each well is 101ul. According to the formula mmol / L=mg / M, each The dose of DCFH-DA in the well is 0.0049216 mg, if these doses are obtained in 1 ul, the concentration of DCFH-DA should be 4.9216 mg / ml, so 5 mg of DCFH-DA was weighed and dissolved in methanol.
[0160] 2. Determination of Propofol on NO Production in Glioma Cells
[0161] After the cells were plated, change the medium (containing 2.0, 6.0, 8.0, an...
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