A method for improving the germination rate of longan seeds stored at room temperature for a short period of time

A germination rate and seed technology is applied in the field of improving the germination rate of longan seeds stored in short-term room temperature, which can solve the problems of low seed germination rate, and achieve the effects of improving the germination rate, high application and economic value, and simple operation.

Active Publication Date: 2020-08-28
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can solve a series of problems caused by the low germination rate of seeds stored at room temperature to a certain extent.

Method used

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  • A method for improving the germination rate of longan seeds stored at room temperature for a short period of time
  • A method for improving the germination rate of longan seeds stored at room temperature for a short period of time
  • A method for improving the germination rate of longan seeds stored at room temperature for a short period of time

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Effect test

Embodiment 1

[0021] A method for improving the germination rate of longan seeds stored at room temperature, the specific steps are:

[0022] 1. Collection and cleaning of longan seeds

[0023] Take the freshly picked "Dongbi" longan fruit, remove the peel and pulp, put the seeds in clean water, remove deformed, germinated, small and light seeds, quickly wash to remove the pulp attached to the seeds, and drain;

[0024] 2. Preparation of Pyromorpha indica Fermented Liquid

[0025] Activate the Pyromorpha indica strain, then pick the aerial hyphae at the edge of the colony and inoculate it on a new PDA solid medium. Place it in an incubator at 28°C for dark culture for 5 days, use a puncher with a diameter of 5 mm to take the bacterial block into a 250 ml Erlenmeyer flask filled with 100 ml of sterilized PDA liquid medium, at a temperature of 28°C, Cultivate in a shaker with a rotation speed of 120 r / min for 2 days, collect the filtrate after filtering through a single layer of gauze, and ...

Embodiment 2

[0032] A method for improving the germination rate of longan seeds stored at room temperature, the specific steps are:

[0033] 1. Collection and cleaning of longan seeds

[0034] Take the freshly picked "Mid-Autumn No. 1" longan fruit, remove the peel and pulp, put the seeds in clean water, remove deformed, germinated, small, and light seeds, quickly wash and remove the pulp attached to the seeds, and drain ;

[0035] 2. Preparation of Pyromorpha indica Fermented Liquid

[0036] Activate the Pyromorpha indica strain, then pick the aerial hyphae at the edge of the colony and inoculate it on a new PDA solid medium. Place it in an incubator at 28°C for dark culture for 5 days, use a puncher with a diameter of 5 mm to take the bacterial block into a 250 ml Erlenmeyer flask filled with 100 ml of sterilized PDA liquid medium, at a temperature of 28°C, Cultivate in a shaker with a rotation speed of 120 r / min for 2 days, collect the filtrate after filtering through a single layer ...

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Abstract

The invention belongs to the technical field of seedling culture of longan, and particularly relates to a method for increasing germinating rate of longan seeds under condition of short-time room temperature storage. The method is characterized in that the longan seeds under the condition of short-time room temperature storage are soaked into a piriformospora indica fermenting liquid, and are fetched out for seeding; compared with the longan seeds which are not soaked into the piriformospora indica fermenting liquid, the germinating rate is obviously increased, and the seedling can well grow.The method has the advantages that the preparation of the piriformospora indica fermenting liquid is easy, the feasibility is strong, the operation is simple in the seed treatment process, and the higher application and economic value is realized.

Description

technical field [0001] The invention belongs to the technical field of longan seedling cultivation, and in particular relates to a method for improving the germination rate of longan seeds stored at room temperature for a short period of time. Background technique [0002] Seeds can be divided into normal seeds and recalcitrant seeds according to storage characteristics. Among them, the recalcitrant seeds still have high water content when they mature, are highly sensitive to dehydration, and are not resistant to drying and low temperature, so they are not resistant to storage and have a short life span. Longan seeds are recalcitrant seeds, and they only have a lifespan of 8-10 days under normal conditions. In order to improve the germination rate of stored seeds, low-temperature or ultra-low temperature storage at ~15°C is often used, but these storage methods require specific storage equipment or low-temperature environments. Due to the large number of longan seeds, this ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00C12N1/14C12R1/645
CPCA01C1/00C12N1/14
Inventor 程春振孙雪丽郝向阳项蕾蕾李丹赖钟雄林玉玲张梓浩刘彦英姚文武竞超
Owner FUJIAN AGRI & FORESTRY UNIV
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