Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Forensic medicine compound detection kit based on 55 Y chromosome SNP genetic markers

A genetic marker and Y chromosome technology, applied in the field of forensic medicine, has achieved good application prospects and promotion value, high system resolution ability, and accurate typing effects

Active Publication Date: 2021-01-26
SICHUAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in relevant literature that there are certain differences in mitochondrial SNPs between the northern and southern Han in China, but the difference in Y-SNP has not been reported yet, and about 80% of the Chinese Han population is distributed in the O-M175 haplogroup and its subgroups

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Forensic medicine compound detection kit based on 55 Y chromosome SNP genetic markers
  • Forensic medicine compound detection kit based on 55 Y chromosome SNP genetic markers
  • Forensic medicine compound detection kit based on 55 Y chromosome SNP genetic markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The preparation of embodiment 1 kit of the present invention

[0075] The composite detection kit for Han male haplogroup subgroup division based on Y chromosome SNP can include the following reagents packaged separately:

[0076] a) Multiplex amplification primer mix. It is obtained by mixing the amplification primers shown in Table 3 and synthesized by Invitrogen Company. The 55 pairs of synthetic amplification primers are prepared with ultrapure water to 100pM / μL, and then mixed according to the ratio in Table 7 to make a composite amplification Primer mix.

[0077] b) Compound amplification reaction mixture. In this embodiment, the PCR reaction mixture MultiplexPCR Mix from Qiagen Company was used.

[0078] c) Multiple single base extension reaction primer mix. It was obtained by mixing the single-base extension reaction primers shown in Table 4, and was synthesized by Invitrogen Company. The synthesized 55 single-base extension reaction primers were configured...

Embodiment 2

[0088] Example 2 Using the kit of the present invention to detect 1028 unrelated Han male individual specimens

[0089] Using the above-mentioned Han male haplogroup subgroup classification composite detection kit based on 55 Y chromosome SNPs, 1028 Chinese Han male unrelated individuals classified into O-M175 haplogroup were detected. The specific detection process is as follows:

[0090] a. Using the Chelex-100 method to extract DNA from the blood samples of 1028 unrelated Chinese Han male individuals as a template for multiplex amplification;

[0091] b. Using the DNA template in step a, use the composite amplification primer mixture and the composite amplification reaction mixture to perform composite PCR amplification on the sample in the following amplification system. System: multiplex amplification primer mixture 0.28 μL, multiplex amplification reaction mixture 2.5 μL, template DNA 1 μL, add ddH 2 0 to 5 μL; 95°C, 15 minutes; 94°C, 30 seconds, 66°C (falling PCR, eve...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of forensic medicines, and concretely relates to a forensic medicine composite examination kit for dividing a China south and north Han male group to O-M175 haplogroup subgroups based on 55 Y-chromosome SNP (Single Nucleotide Polymorphism) genetic markers. The invention aims to solve the technical problem so as to utilize the Y-chromosome SNP genetic markers for further subdividing positions of examined materials from the China south and north Han male group divided to the O-M175 haplogroup on a Y-chromosome evolution tree. The invention provides the technical scheme that the forensic medicine composite examination kit based on the 55 Y-chromosome SNP genetic markers comprises a multiplex amplification primer mixture and a multiple single base extension reaction primer mixture packed separately. The kit provided by the invention applies a multiplex amplification technology and a multiple single base extension technology, so that gene types of the 55 Y-chromosome SNP genetic markers of the biological examined materials can be obtained at the same time, and male samples from the China south and north Han male group divided to the O-M175 haplogroup can be correctly classified to the subgroups of the existing acknowledged O-M175 haplogroup.

Description

technical field [0001] The invention belongs to the technical field of forensic medicine, and in particular relates to a forensic medicine composite detection kit for dividing Han male populations in northern and southern China into O-M175 haplogroup subgroups based on 55 Y chromosome SNP genetic markers. Background technique [0002] Single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) refers to the DNA sequence polymorphism caused by the variation of a single base sequence in a specific part of the genome, which widely exists in the human genome. Most of the SNPs are biallelic markers, which have the characteristics of small amplified fragments, low mutation rate, stable genetics, and easy automatic typing. They are suitable for degraded DNA samples, phenotype prediction, and ethnic origin inference. [0003] The Y chromosome is a chromosome unique to males. Due to the different genetic patterns, the Y chromosome can be divided into pseudo-autosomal region...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 侯一平郎敏叶懿李介男宋凤谢明坤王正刘京罗海玻李英碧
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com